| 质粒名称: | pADH140 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90988 |
| 存储实验室: | Aaron Hernday |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | Clontech |
| 空载体大小: | 2242bp |
| 完整质粒大小: | 9184bp |
| 载体类型: | CRISPR |
| 筛选标记: | Nourseothricin (NAT) |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | C. maltosa LEU2 1 of 2 |
| 插入大小: | 750bp |
| 插入基因名称2: | pENO1 |
| 插入大小: | 783bp |
| 插入基因名称3: | Cas9 |
| 插入大小: | 4113bp |
| 插入基因名称4: | NAT 1 of 2 |
| 插入大小: | 842bp |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pADH140 was a gift from Aaron Hernday (Addgene plasmid # 90988 ; http://n2t.net/addgene:90988 ; RRID:Addgene_90988) |
| 参考文献部分: | An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans. Nguyen N, Quail MMF, Hernday AD. mSphere. 2017 Apr 26;2(2):e00149-17. doi: 10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr. 10.1128/mSphereDirect.00149-17 PubMed 28497115 |
买家导航
pADH140载体质粒基本信息
pADH140质粒图谱载体图谱和pADH140载体序列质粒序列多克隆位点信息
pADH140质粒载体简介
pADH140质粒序列载体序列
LOCUS pADH140 9185 bp DNA circular SYN 14-APR-2025
DEFINITION NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT
CRISPR system. Use with pADH143-series gRNA expression constructs..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9185)
AUTHORS Nguyen N, Quail MMF, Hernday AD
TITLE An Efficient, Rapid, and Recyclable System for CRISPR-Mediated
Genome Editing in Candida albicans.
JOURNAL mSphere. 2017 Apr 26;2(2):e00149-17. doi:
10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr.
PUBMED 28497115
REFERENCE 2 (bases 1 to 9185)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1128/mSphereDirect.00149-17"; journalName: "mSphere"; date:
"2017-04-26- 26"; volume: "2"; issue: "2"; pages: "e00149-17"
COMMENT Sequence Label: pADH140
FEATURES Location/Qualifiers
source 1..9185
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 205..224
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(443..547)
/gene="bla"
/label=AmpR promoter
primer_bind 615..633
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
CDS 2201..6304
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 2201..6304
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 6317..6337
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6341..6361
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6365..6385
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6386..6451
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 6516..6763
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(6532..6550)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
promoter 6803..7146
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
promoter complement(join(7654..7660,7661..7678,7679..7684))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7699..7720
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7837..7854)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8008..8596)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8088..8107)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(8767..9185,1..373,374..442))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
61 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
121 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
181 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
241 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
301 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
361 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
421 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
481 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
541 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
601 cattaaccta taaaaatagg cgtatcacga ggccctttga cggcacggcc acgcgtttaa
661 accgccatgt cagttaaaac taaaactatt acaattttac caggtgatca cgtcggtacc
721 gaaattgtca atgaagctat caaagtttta gaagcaattg aagctgccac tccttaccaa
781 aaaatccatt tcgatttcaa acatcattta atcggtggtg ctgccattga tgccactggt
841 gtcccattgc cagatgacgc acttgaaagt gccaaaaaat ctgatgccgt cttattgggt
901 gctgttggtg gaccaaaatg ggggactggc gccgttcgtc cagaacaagg tttattaaag
961 attcgtaaag aattgaacct ttatgctaac atcagaccat gtaactttgc cagtgactcc
1021 ttattggaat tatccccatt gagaccagaa gtcgttaaag gtacaaactt gatcattgtt
1081 cgtgaattag ttggtggtat ttatttcggt gatcgtgaag aacaagaaga aagtgaagat
1141 aaacaaaccg catgggatac tgaaaaatac accgtcgatg aagtcaccag aatcacccgt
1201 atggctgcct tcatggcttt acaacataac ccaccattac caatctggtc attggataag
1261 gctaatgttt tagcttcttc tagattatgg agaagaactg tcgataaagt gatttctgaa
1321 gagttcccaa ctttgtctgt tcaacatcaa ttgattgact ccgccgccat gattttaatt
1381 caaaacccaa ccaaattgaa tggtataatc atcactacag ctaacaatta cacaaaaaaa
1441 attctctttt cgatgtagca actatcttca actaaaacat tttctccttc ggcccatgat
1501 tgtcctccgg gtcgacagca agccgttaca attgagatgg aaagcgacct accttcactc
1561 gataaggtgc ttaattgtac ttcatataaa tctggcccgg atctaaacaa atgagttcca
1621 ttaagccgtg ggttctcaat tagggttttt gtttttgatt tagaaaaaag agatcaagat
1681 ttgtttacag gtgatgcctt tttttagaac ttatgcgttg caaaagttga ctaacgattt
1741 ctataaggtg atccacacta attatacaaa cgtacaaaca gacatacttt tcctgcgttc
1801 acctgatgtt ggccagattt ctctcttcat tgcatagaac ataaccacac tagggcaaca
1861 gaaaaaaaaa aaaaagtgca tcgggaagtt gtgttccatt cattatatgt ctactactgc
1921 atatgagtag cccacccacc accaccatag taagtttttg tgtatgcgcg ccgtcaggtt
1981 atttcatttc tgaatttttc aaccacctta ctccctttat tgttgattga caattttgct
2041 cacagtaaga tcttttagac tccaattaat ataaaataag tctgattttc caattcctgt
2101 tttttctttt tttttctgtt tctatttctt tccttttctc cctttttttt aattcttcat
2161 tcaatcatca attgataatt caggaatatt acaacccggg atggataaaa agtatagtat
2221 tggtttagat attggtacta actctgtggg ttgggcagtt atcactgacg aatataaagt
2281 tccatcaaag aaatttaagg tgttaggtaa cactgacaga cactcaataa aaaagaatct
2341 tatcggtgct cttttgttcg actccggtga aactgccgag gctacacgtt taaaaagaac
2401 agcaagaaga agatataccc gtagaaaaaa tagaatatgt tatttacaag aaatcttttc
2461 taatgaaatg gctaaagttg atgattcctt tttccataga ttggaagagt catttttggt
2521 tgaagaagac aaaaagcatg agagacatcc aatctttggg aatatagttg atgaagtggc
2581 ttaccatgaa aaatatccta ccatttatca tttaagaaag aaattggtag attcaactga
2641 taaagctgac cttagattaa tctatttagc acttgcccat atgattaaat ttagaggtca
2701 ttttttgatt gaaggtgatt tgaacccaga taattctgac gtggataaat tatttattca
2761 attagtccaa acctacaacc aattatttga ggaaaatcca attaatgcta gtggtgtcga
2821 tgccaaagct atattatcag ccagattatc aaaatctaga cgtttggaaa atttgattgc
2881 ccaattgcca ggagaaaaaa agaatggatt atttggaaac ttgatcgcat tatcattggg
2941 tttgacacca aattttaaat ctaattttga tttagctgaa gatgctaaat tacaattatc
3001 aaaagacacc tatgacgacg atttggacaa tttacttgct caaattggtg atcaatatgc
3061 agatttgttc ttagctgcta aaaacttatc tgatgctatt ttgttgtctg atattttgag
3121 agtgaacaca gaaataacca aagctccatt atcagcatct atgatcaaac gttatgatga
3181 acaccatcag gatttgactt tattgaaagc tttggtgaga caacaattgc cagagaagta
3241 taaagaaatc tttttcgatc aatctaaaaa cgggtatgca ggttatattg atgggggtgc
3301 ctcccaagag gaattttaca aatttataaa acctatttta gaaaagatgg atgggactga
3361 ggaacttttg gtcaaattga acagagaaga tttgttacgt aaacagagaa cttttgataa
3421 tggtagtata cctcaccaaa ttcatttggg tgagttgcat gcaattttaa gaagacaaga
3481 agatttttat ccatttttaa aagataatag agaaaaaatc gagaaaattt taacctttag
3541 aattccatac tatgttgggc ctttggctag aggtaattca agatttgcct ggatgacacg
3601 taaatcagaa gaaactatta ccccttggaa ttttgaagag gttgttgata aaggagcatc
3661 agcacagagt tttattgaaa gaatgaccaa tttcgataaa aacttaccaa atgaaaaagt
3721 tttaccaaaa cattccttgt tatacgaata ttttactgtt tacaatgaac ttacaaaggt
3781 taaatatgtt actgaaggta tgcgtaagcc agccttttta tctggagaac agaaaaaggc
3841 aatagttgat ttattgttta aaacaaatag aaaagttact gttaaacaat taaaagaaga
3901 ttactttaag aaaattgaat gttttgattc agttgaaatc agtggtgttg aagacagatt
3961 taatgctagt ttaggaactt accatgattt acttaaaatt atcaaagata aagatttctt
4021 ggataacgaa gaaaatgaag acattttaga agacattgtt ttaaccttaa ctttattcga
4081 agatagagag atgattgaag aacgtttgaa gacttatgca catttgtttg acgataaagt
4141 gatgaaacag ttgaaaagaa gacgttatac tggatggggt agattgtctc gtaaattgat
4201 caatggaatt agagataaac aaagtggtaa aactatcttg gactttttga aatctgacgg
4261 atttgctaat agaaatttca tgcaattgat ccacgacgat agtttgacat ttaaagaaga
4321 catccaaaag gcccaagtga gtgggcaagg tgattcatta catgaacata ttgcaaattt
4381 agccggatct cctgctatta agaaagggat attacaaact gttaaagttg tggatgaatt
4441 agtgaaagta atgggaagac ataaacctga aaacattgtc attgagatgg caagagaaaa
4501 tcaaactaca caaaaaggac agaaaaatag tagagaacgt atgaaaagaa tagaagaggg
4561 tattaaagaa ttgggtagtc aaatattgaa agaacaccca gtggaaaata cccagttgca
4621 aaatgaaaaa ttatatcttt actaccttca aaatggacgt gatatgtatg ttgatcagga
4681 attagatata aatagacttt cagattatga tgtagatcat atagttccac aatctttctt
4741 gaaagatgat tccatagaca ataaagtatt aactagaagt gataaaaata gaggtaaaag
4801 tgataatgtc ccaagtgagg aagtcgtcaa aaagatgaaa aattactggc gtcaactttt
4861 gaatgctaaa ttaattactc aaagaaaatt tgataatttg actaaagcag aaagaggtgg
4921 gctttctgaa ttagataaag ccgggttcat taaaagacaa ttggtcgaaa ctagacaaat
4981 tactaaacat gttgcccaaa ttttagattc ccgtatgaac actaagtatg acgaaaatga
5041 taagttaata cgtgaggtta aagtcattac tttaaaatca aaacttgtct ctgatttcag
5101 aaaggatttc caattctata aagttagaga aattaataat tatcatcatg ctcatgatgc
5161 atatttgaat gctgtagttg gaactgcttt aatcaagaaa taccctaaat tagaatctga
5221 atttgtatat ggtgattaca aagtctatga tgttagaaag atgattgcta aatcagaaca
5281 agaaattggt aaagctacag ctaaatactt cttttactct aacattatga atttctttaa
5341 aacagaaatt actttggcaa acggtgaaat tagaaaaaga cctcttattg aaacaaatgg
5401 tgagactgga gagatagttt gggacaaagg gcgtgatttc gctactgtta gaaaagtttt
5461 atcaatgcca caagttaaca ttgtaaagaa aacagaggtt caaactggtg gtttctcaaa
5521 agaaagtatt ttgcctaaaa gaaatagtga taaattgatt gccagaaaaa aggattggga
5581 tccaaagaaa tatggtggtt tcgactcacc aaccgtagcc tattctgttt tggttgtggc
5641 aaaggttgaa aagggtaaaa gtaaaaagct taaatcagta aaagaacttt tgggtattac
5701 aataatggaa agaagttcct ttgaaaagaa ccctattgat tttttggaag ctaaaggtta
5761 taaggaagta aagaaggact taataatcaa attgcctaaa tattctttat ttgaattaga
5821 aaatgggaga aaaagaatgt tggcttctgc tggagaattg caaaagggta atgaattagc
5881 attgccttcc aaatatgtta acttcttgta tttagcttca cactatgaaa agttgaaagg
5941 gtcaccagaa gataacgagc aaaaacaatt atttgttgaa caacacaaac actacttaga
6001 tgagattata gaacaaatta gtgaattcag taaaagagtg atattagctg atgcaaattt
6061 agataaagtt ttgtcagcct ataacaaaca tagagataag ccaattagag aacaagcaga
6121 aaacattatt cacttattta cccttaccaa tttaggagca cctgctgctt tcaagtattt
6181 tgatacaaca attgatcgta aaagatatac ctcaacaaaa gaagtcttag acgccacctt
6241 aattcatcaa tcaatcactg gattgtatga gacaagaatt gatttgtctc aattgggtgg
6301 tgatgaaggg gctgatccta agaagaaaag aaaagttgat ccaaagaaaa agcgtaaggt
6361 ggatcctaag aaaaagagaa aggttgacta caaagaccat gacggtgatt ataaagatca
6421 tgacatcgac tacaaggatg acgatgacaa gtgataatga ctgcagagat ccatcgacct
6481 gccgccaagc taattccggg cgaatttctg tcgagtcatg taattagtta tgtcacgctt
6541 acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga
6601 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
6661 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
6721 cttgctttag aaggttttgg gacgctcgaa ggctttaatt tgcggccagc ttgcctcgtc
6781 cccgcctggt cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga
6841 cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca
6901 tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg
6961 ctcctcgctg cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc
7021 ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt
7081 tcatatactt ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa
7141 acaaccggat cctacaatta tgtctactac tttggatgat actgcttata gatacagaac
7201 ttctgttcca ggtgatgctg aagctattga agctttggat ggttctttca ctaccgatac
7261 tgtttttaga gttactgcta ctggtgatgg tttcactttg agagaagttc cagttgatcc
7321 accattgact aaggttttcc cagatgatga atccgatgat gaatccgatg ctggtgaaga
7381 tggtgatcca gattctagaa ctttcgttgc ttatggtgat gatggtgatt tggctggttt
7441 cgttgttgtt tcttattctg gttggaacag aagattgact gttgaagata ttgaagttgc
7501 tccagaacat agaggtcatg gtgttggtag agctttgatg ggtttggcta ctgaattcgc
7561 cagagaaaga ggtgctggtc atttgtggtt ggaagttacc aatgttaatc ggtgtttaaa
7621 ccccagcgcc tggcgggcgc tcacaattcc acacaacata cgagccggaa gcataaagtg
7681 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
7741 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
7801 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
7861 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
7921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
7981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
8041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
8101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
8161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
8221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
8281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
8341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
8401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
8461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
8521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
8581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
8641 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
8701 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
8761 tgacagttac caatgcttga tcagtgaggc acctatctca gcgatctgtc tatttcgttc
8821 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
8881 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
8941 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
9001 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
9061 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
9121 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
9181 aaaag
//
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