| 质粒名称: | pAAV-beta-arrestin2-HA-TEV219 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 104845 |
| 存储实验室: | Alice Ting |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 载体出品公司: | Clontech |
| 空载体大小: | 5774bp |
| 完整质粒大小: | 5774bp |
| 载体类型: | 哺乳细胞表达, AAV |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | beta-arrestin2-HA-TEV219 |
| 物种来源: | Synthetic |
| 启动子: | CMV |
| 克隆方法: | 未知 |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-beta-arrestin2-HA-TEV219 was a gift from Alice Ting (Addgene plasmid # 104845 ; http://n2t.net/addgene:104845 ; RRID:Addgene_104845) |
| 参考文献部分: | Time-gated detection of protein-protein interactions with transcriptional readout. Kim MW, Wang W, Sanchez MI, Coukos R, von Zastrow M, Ting AY. Elife. 2017 Nov 30;6. doi: 10.7554/eLife.30233. 10.7554/eLife.30233 |
买家导航
pAAV-beta-arrestin2-HA-TEV219载体质粒基本信息
pAAV-beta-arrestin2-HA-TEV219质粒图谱载体图谱和 pAAV-beta-arrestin2-HA-TEV219载体序列质粒序列多克隆位点信息
pAAV-beta-arrestin2-HA-TEV219质粒载体简介
pAAV-beta-arrestin2-HA-TEV219质粒序列载体序列
LOCUS pAAV-beta-arrestin2-HA-TEV219 5774 bp DNA circular SYN 15-DEC-2025
DEFINITION b2AR-SPARK protease component.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5774)
AUTHORS Kim MW, Wang W, Sanchez MI, Coukos R, von Zastrow M, Ting AY
TITLE Time-gated detection of protein-protein interactions with
transcriptional readout.
JOURNAL Elife. 2017 Nov 30;6. doi: 10.7554/eLife.30233.
PUBMED 29189201
REFERENCE 2 (bases 1 to 5774)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Elife.";
date: "2017-11-30"; volume: "6. doi"; pages: " 10.7554/eLife.30233"
COMMENT Sequence Label: pAAV-beta-arrestin2-HA-TEV219
FEATURES Location/Qualifiers
source 1..5774
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
enhancer 201..504
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 505..708
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 659..679
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 753..772
/label=T7
/note="T7 promoter, forward primer"
promoter 753..771
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 785..794
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2021..2047
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 2048..2074
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(2795..2812)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2801..3008
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 3037..3177
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3037..3166
/label=AAV2 ITR
rep_origin 3252..3707
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3339..3358)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3549..3570
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3724..3743)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3843..3865
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3903..3921)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3989..4093
/gene="bla"
/label=AmpR promoter
CDS join(4094..4162,4163..4954)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4312..4331)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5125..5713
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5614..5633
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgttgtaca tagtaatcaa ttacggggtc attagttcat
181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
301 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
721 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
781 gcttgccacc atgggtgaaa aacccgggac cagggtcttc aagaagtcga gccctaactg
841 caagctcacc gtgtacttgg gcaagcgtga ctttgtggat cacttggaca aagtggatcc
901 tgtcgatggt gtggtgcttg tggatcctga ctacttgaag gaccggaaag tgtttgtgac
961 cctcacctgt gccttccgct atggccgaga agacctggat gtactgggcc tgtctttccg
1021 caaagatctg ttcatcgcca cctaccaggc cttccccccc atgcccaacc cacctcggcc
1081 ccccacccgc ctacaggacc gactgctgaa gaagttgggc cagcacgccc accccttttt
1141 tttcacaata ccccagaatt tgccttgctc cgtcacactg cagccaggac cggaggacac
1201 agggaaggcc tgtggagtag actttgagat tcgagccttc tgtgccaaat ctatagaaga
1261 aaaaagccac aaaaggaact ccgtgcggct tatcatcaga aaggtacagt ttgctcctga
1321 gacacccggc ccccagccat cagctgaaac cacacgccac ttcctcatgt ctgaccggag
1381 gtccctgcac ctagaggctt ccctggacaa agagctgtac taccatgggg aacccctcaa
1441 tgtcaacgtc cacgtcacca acaattctgc caaaaccgtc aagaagatca gagtgtctgt
1501 gagacagtat gccgacattt gcctcttcag caccgcgcag tacaagtgtc ctgtggctca
1561 gcttgaacaa gatgaccagg tgtctcccag ttccacattc tgcaaggtgt acaccataac
1621 cccgctgctc agtgacaacc gagagaagcg tggccttgcc cttgatgggc aactcaagca
1681 cgaagacacc aacctggctt ccagcaccat tgtgaaggag ggagccaaca aggaggtgct
1741 gggaatccta gtatcctaca gggtcaaggt gaagctggtg gtgtctcgag gcggggatgt
1801 ctccgtggag ctacctttcg tcctaatgca ccccaagccc cacgaccaca tcacccttcc
1861 ccgaccccag tcagcccccc gggaaataga catccctgtg gataccaacc tcattgaatt
1921 cgataccaac tatgccacag acgacgacat cgtgtttgag gactttgcga ggcttcggct
1981 gaaggggatg aaggatgacg actgtgatga ccagttctgc tacccatacg atgtgccaga
2041 ttacgcctat ccctatgacg tccctgatta cgctggtgga agtggatcag gcagcggtgg
2101 atctggagaa agcttgttta agggaccacg tgattacaac ccgatatcga gcaccatttg
2161 tcatttgacg aatgaatctg atgggcacac aacatcgttg tatggtattg gatttggtcc
2221 cttcatcatt acaaacaagc acttgtttag aagaaataat ggaacactgt tggtccaatc
2281 actacatggt gtattcaagg tcaagaacac cacgactttg caacaacacc tcattgatgg
2341 gagggacatg ataattattc gcatgcctaa ggatttccca ccatttcctc aaaagctgaa
2401 atttagagag ccacaaaggg aagagcgcat atgtcttgtg acaaccaact tccaaactaa
2461 gagcatgtct agcatggtgt cagacactag ttgcacattc ccttcatctg atggcatatt
2521 ctggaagcat tggattcaaa ccaaggatgg gcagtgtggc agtccattag tatcaactag
2581 agatgggttc attgttggta tacactcagc atcgaatttc accaacacaa acaattattt
2641 cacaagcgtg ccgaaaaact tcatggaatt gttgacaaat caggaggcgc agcagtgggt
2701 tagtggttgg cgattaaatg ctgactcagt attgtggggt ggccataaag ttttcatggt
2761 gtaagaattc ctcgagctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
2821 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
2881 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
2941 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
3001 gctggggagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
3061 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
3121 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
3181 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
3241 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
3301 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
3361 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
3421 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
3481 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
3541 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
3601 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
3661 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
3721 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
3781 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
3841 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
3901 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
3961 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
4021 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
4081 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
4141 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
4201 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
4261 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
4321 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
4381 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
4441 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
4501 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
4561 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
4621 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
4681 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
4741 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
4801 tgagcgtgga agccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
4861 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
4921 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
4981 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
5041 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
5101 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
5161 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
5221 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
5281 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
5341 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
5401 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
5461 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
5521 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
5581 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
5641 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
5701 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
5761 ttttgctcac atgt
//
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