pAAVLINKv2-SCP1-Cre-UDeg3a-SRCAP-C-FLAG

LOCUS      pAAVLINKv2-SCP1-Cre-UDeg3a-SRCAP-C-FLAG               7234 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 17, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3938
                     /codon_start=2
                     /gene="SRCAP"
                     /gene_synonym="DEHMBA; DOMO1; EAF1; FLHS; SWR1"
                     /product="helicase SRCAP"
                     /label=SRCAP
                     /note="Snf2-related CBP activator protein;Swi2/Snf2-related
                     ATPase homolog, domino homolog 1; domino homolog 2"
                     /db_xref="GI:146219843"
                     /db_xref="CCDS:CCDS10689.2"
                     /db_xref="GeneID:10847"
                     /db_xref="HGNC:HGNC:16974"
                     /db_xref="MIM:611421"
                     /protein_id="NP_006653.2"
                     /translation="ERVPRPAPRPRPTPASAPAAIPALVPVPVSAPVPISAPNPITILP
                     VHILPSPPPPSQIPPCSSPACTPPPACTPPPAHTPPPAQTCLVTPSSPLLLGPPSVPIS
                     ASVTNLPLGLRPEAELCAQALASPESLELASVASSETSSLSLVPPKDLLPVAVEILPVS
                     EKNLSLTPSAPSLTLEAGSIPNGQEQEAPDSAEGTTLTVLPEGEELPLCVSESNGLELP
                     PSAASDEPLQEPLEADRTSEELTEAKTPTSSPEKPQELVTAEVAAPSTSSSATSSPEGP
                     SPARPPRRRTSADVEIRGQGTGRPGQPPGPKVLRKLPGRLVTVVEEKELVRRRRQQRGA
                     ASTLVPGVSETSASPGSPSVRSMSGPESSPPIGGPCEAAPSSSLPTPPQQPFIARRHIE
                     LGVTGGGSPENGDGALLAITPPAVKRRRGRPPKKNRSPADAGRGVDEAPSSTLKGKTNG
                     ADPVPGPETLIVADPVLEPQLIPGPQPLGPQPVHRPNPLLSPVEKRRRGRPPKARDLPI
                     PGTISSAGDGNSESRTQPPPHPSPLTPLPPLLVCPTATVANTVTTVTISTSPPKRKRGR
                     PPKNPPSPRPSQLPVLDRDSTSVLESCGLGRRRQPQGQGESEGSSSDEDGSRPLTRLAR
                     LRLEAEGMRGRKSGGSMVVAVIQDDLDLADSGPGGLELTPPVVSLTPKLRSTRLRPGSL
                     VPPLETEKLPRKRAGAPVGGSPGLAKRGRLQPPSPLGPEGSVEESEAEASGEEEEGDGT
                     PRRRPGPRRLVGTTNQGDQRILRSSAPPSLAGPAVSHRGRKAKT"
     misc_feature    1707..1712
                     /label=SDS
     misc_feature    1722..1727
                     /label=SDS
     misc_feature    1749..1754
                     /label=SDS
     misc_feature    1752..1757
                     /label=SDS
     misc_feature    1969..1974
                     /label=SDS
     misc_feature    2113..2118
                     /label=SDS
     CDS             complement(2202..2213)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    2403..2408
                     /label=SDS
     misc_feature    3035..3040
                     /label=SDS
     misc_feature    3784..3789
                     /label=SDS
     misc_feature    3787..3792
                     /label=SDS
     CDS             3942..4007
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4023..4454
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4167..4172
                     /label=SDS
     repeat_region   4497..4637
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4497..4626
                     /label=AAV2 ITR
     repeat_region   4498..4638
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4712..5167
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5449..5553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5554..6414
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6585..7173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggag cgagttccca
     1561 ggccagcacc taggcctcga cccactccag cttcagctcc ggctgcaatt cctgcccttg
     1621 ttcctgtccc agtttctgcc ccagtaccca tttcagcccc aaatccaata accattctcc
     1681 ctgtccatat cttgccttct cctccccctc cttcacagat tcctccttgt tcttctcctg
     1741 cctgcacccc tcctcctgcc tgtacccctc caccagctca tacaccgcct ccagcccaaa
     1801 cctgtcttgt aactccttcc tctcctctct tgcttggtcc accttctgtg cccatctctg
     1861 cctcagtcac taatctcccc ttgggcttga ggcctgaggc agagctgtgt gcccaggcat
     1921 tggcatctcc agagtccctg gagctggctt ctgtggccag ttcagaaacc tcctcacttt
     1981 ctcttgtgcc ccctaaagat ctgttgccag ttgctgtgga gatcctgcct gtgtcagaga
     2041 agaacctttc tctcacccct tctgcaccca gcctgacctt ggaggctggc agcatcccca
     2101 atggtcaaga gcaggaggca ccagattctg ctgaggggac cacccttaca gtgctgcctg
     2161 aaggtgagga gttgcccctg tgtgtgagtg agagcaatgg cctggagctc ccaccctcag
     2221 cagcatctga tgagccactt caggagccac tggaggctga caggacctcg gaagagctga
     2281 cagaggccaa gaccccaacc tccagcccag agaagccaca ggaactcgtt acagctgagg
     2341 ttgcagctcc atccacctca tcttcagcca cttcctcgcc tgagggtcct tcacctgccc
     2401 gacctcctcg gcgtcgcacc agtgctgatg tggaaattag gggtcaaggg actggtcggc
     2461 caggacaacc accaggcccc aaagtgcttc gaaagctgcc aggacggctg gtaactgtgg
     2521 tagaggaaaa ggaactggtg cggcggcggc ggcagcagcg gggagctgcc agcaccctag
     2581 tgcctggggt ctctgagact agtgccagcc cgggaagccc gtctgtccgc agcatgtcag
     2641 ggccagaatc ctcccctccc attggtgggc cctgtgaagc tgctccttca tcctcactgc
     2701 ccactccacc ccagcagccc ttcattgctc gccgtcacat tgagctgggg gtgactggtg
     2761 gtggcagccc cgagaatgga gacggagcac tgctcgccat caccccacct gctgtgaaac
     2821 gtcggagggg gaggcccccc aagaagaaca ggtctccagc agatgctggg agaggtgtgg
     2881 atgaggcacc ctcatccacc ttgaagggaa aaaccaatgg ggctgaccca gtccctgggc
     2941 ctgagaccct aattgttgca gatcctgtcc tggaaccaca gcttattcct gggccccagc
     3001 ctcttggacc ccagccagtt cacagaccca atcccctcct gtcacctgtg gagaaaagaa
     3061 ggcgaggacg accccctaaa gcacgagatt tgcccatccc tgggaccatt tcctctgcag
     3121 gggatggcaa ctccgaaagt cggacacagc cacccccaca cccatcaccc ctaaccccac
     3181 tcccaccact gctagtttgt cccactgcta ctgttgccaa cactgtcacc actgtcacca
     3241 tttcaacgtc cccacccaaa cggaagaggg gccgacctcc caagaatcct ccatcacctc
     3301 ggcccagcca gctccccgtc ttggaccgtg acagcacttc tgttctcgag agctgtggat
     3361 tggggaggcg acggcaaccc cagggccaag gggagagtga gggtagttcc tctgatgagg
     3421 atggaagccg ccccctcacc cgcctggccc gccttcggct tgaagcagaa ggaatgcgag
     3481 gacggaagag tggagggtcc atggtggtgg ctgtaattca ggatgacctg gacttagcag
     3541 atagcgggcc aggcgggttg gaattgacac cacctgtggt ctcactaacc ccaaaactgc
     3601 gctcgacccg gctgcgtcca gggtctctag tccccccact agagactgag aagttgcctc
     3661 gcaaacgagc aggggcccca gttggtggga gtcctgggct ggcaaagcgg ggccgcctac
     3721 agcccccaag tcccctgggg cctgagggtt cagtagagga gtctgaggct gaagcctcag
     3781 gtgaggagga ggaaggggat gggaccccac gccgacgtcc tggcccccgc cggcttgttg
     3841 ggaccaccaa ccaaggggac cagcgcatcc tgcgcagcag cgcccctccc tccctggctg
     3901 gccctgctgt tagtcacaga ggccgcaagg ccaagacgaa ggactacaaa gaccatgacg
     3961 gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtaa gaattctgat
     4021 gactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4081 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4141 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac
     4201 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4261 tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4321 ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     4381 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     4441 gggaggtttt ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga
     4501 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4561 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4621 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4681 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4741 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     4801 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4861 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4921 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4981 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5041 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     5101 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     5161 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     5221 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5281 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5341 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5401 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5461 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5521 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5581 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5641 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5701 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5761 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5821 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5881 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5941 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6001 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6061 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6121 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6181 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6241 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6301 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6361 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6421 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6481 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6541 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6601 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6661 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6721 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6781 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6841 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6901 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6961 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7021 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7081 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7141 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7201 ttcctggcct tttgctggcc ttttgctcac atgt
//