pYK472

LOCUS       pYK472               7128 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     880..946
                     /label=oYF0019
     primer_bind     880..899
                     /label=oSCL1613
     primer_bind     881..918
                     /label=oYK421_GNCR_F
     primer_bind     881..902
                     /label=oYK31-mCherry-NS3a-F
     primer_bind     910..952
                     /label=oYF005
     primer_bind     916..937
                     /label=oYK481_GNCR_F
     primer_bind     916..937
                     /label=oYK483_LCK1_F
     primer_bind     916..936
                     /label=oYK490_P2A_GNCR1_F
     CDS             919..1617
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     primer_bind     919..952
                     /label=oYF0011
     primer_bind     919..943
                     /label=oLZ146 GNCR1-N F
     primer_bind     919..943
                     /label=oSCL762
     primer_bind     1585..1597
                     /label=oYK03-02-F
     primer_bind     1585..1596
                     /label=oYK31-mCherry-NS3a-F
     primer_bind     complement(1596..1653)
                     /label=oYF0020
     primer_bind     complement(1596..1647)
                     /label=oYF006
     primer_bind     complement(1596..1618)
                     /label=oYF0012
     primer_bind     complement(1605..1624)
                     /label=oYK465_GNCR1_R
     primer_bind     1615..1637
                     /label=oSCL1129
     primer_bind     1615..1635
                     /label=oLZ147 NS3a F
     primer_bind     1615..1634
                     /label=oYF0033
     primer_bind     complement(1617..1643)
                     /label=oDJ171-4
     primer_bind     1617..1637
                     /label=oLZ048 NSP3-F
     primer_bind     1617..1634
                     /label=oYK419_GS_F
     primer_bind     1618..1695
                     /label=oDJ171-3
     primer_bind     1618..1647
                     /label=oSCL388
     primer_bind     1618..1638
                     /label=oYK451_NLS_F
     primer_bind     complement(1618..1637)
                     /label=oYK438_SV40_R
     primer_bind     1618..1633
                     /label=oYK28-mCherry-NS3a-F
     primer_bind     complement(1619..1667)
                     /label=oLZ145 DNCR2-GNCR1 R
     primer_bind     complement(1625..1643)
                     /label=Oscl18-4
     primer_bind     complement(1628..1648)
                     /label=oSCL1119
     primer_bind     complement(1628..1647)
                     /label=oSCL075
     primer_bind     complement(1628..1647)
                     /label=oSCL075a
     primer_bind     complement(1628..1647)
                     /label=oSCL075`b
     primer_bind     1630..1692
                     /label=oYK1253_mCherry_F
     primer_bind     complement(1631..1647)
                     /label=oYK472_NLS_R
     primer_bind     1632..1652
                     /label=oLZ146 GNCR1-N F
     primer_bind     complement(1632..1648)
                     /label=oYK92_L7Ae_R
     primer_bind     complement(1634..1662)
                     /label=oYK489_GNCR1_R
     misc_feature    1634..1652
                     /label=linker
     primer_bind     complement(1636..1691)
                     /label=oYK75_NLS_mcp_R
     primer_bind     1638..1690
                     /label=oYK03-02-F
     primer_bind     complement(1645..1668)
                     /label=oYK421_GNCR3_R
     primer_bind     1649..1686
                     /label=oYK421_mCherry_F
     misc_feature    1653
                     /label=linker
     misc_feature    1654..1668
                     /label=NSP3
     primer_bind     1669..1692
                     /label=oXQ135
     primer_bind     1669..1692
                     /label=oXQ174
     primer_bind     1669..1692
                     /label=omz_mCherry_F
     primer_bind     1669..1689
                     /label=oYK31-mCherry-NS3a-F
     CDS             1672..2376
                     /codon_start=1
                     /label=mCherry
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1672..1698
                     /label=omz_linker_mCherry_F
     primer_bind     1713..1731
                     
     primer_bind     1713..1731
                     /label=DDL7AeF
                     /note="KpnI"
     primer_bind     1713..1731
                     /label=L7Ae FLP
     primer_bind     complement(1984..1995)
                     /label=oSCL011
     primer_bind     complement(1984..1995)
                     /label=omz_Coh2_R
     primer_bind     complement(1984..1995)
                     /label=omz_Coh2_His_R
     primer_bind     2116..2125
                     /label=oYK92_L7Ae_R
     primer_bind     2117..2133
                     /label=mCherry_F测序
     primer_bind     2185..2198
                     /label=oYK451_NLS_F
     primer_bind     2329..2408
                     /label=oSCL1385
     primer_bind     complement(2356..2445)
                     /label=oYK316_mCherry_CAAX_R
     primer_bind     complement(2356..2379)
                     /label=oXQ136
     primer_bind     complement(2356..2379)
                     /label=oDJ171-4
     primer_bind     complement(2356..2376)
                     /label=oXQ161
     primer_bind     complement(2356..2376)
                     /label=omz_mCherry_His_R
     primer_bind     complement(2356..2376)
                     /label=oYK56-mCherry-R
     primer_bind     complement(2356..2376)
                     /label=oYK58-mCherry-R
     primer_bind     complement(2358..2387)
                     /label=omz_mCherry_linker_R
     primer_bind     complement(2364..2382)
                     /label=oYK431_NES2_R
     primer_bind     complement(2368..2388)
                     /label=oYK489_GNCR1_R
     primer_bind     2369..2407
                     /label=oYK472_NLS_F
     primer_bind     complement(2369..2388)
                     /label=pYK472_mCherry_R
     primer_bind     2377..2388
                     /label=oLZ147 NS3a F
     primer_bind     2389..2427
                     /label=oSCL1385
     primer_bind     2389..2405
                     /label=oSCL1613
     CDS             2398..2445
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     2398..2413
                     /label=oYK451_NLS_F
     primer_bind     2404..2462
                     /label=oSCL1384
     primer_bind     2437..2501
                     /label=oYK419_GS_F
     primer_bind     complement(2457..2504)
                     /label=oYK438_SV40_R
     primer_bind     2459..2504
                     /label=oSCL1129
     primer_bind     2459..2502
                     /label=oLZ147 NS3a F
     primer_bind     2459..2501
                     /label=oYF0033
     primer_bind     complement(2463..2485)
                     /label=oSCL1614
     CDS             2464..2484
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     2485..2521
                     /label=oYK451_NLS_F
     primer_bind     2485..2505
                     /label=oDJ171-3
     primer_bind     2485..2505
                     /label=oSCL388
     primer_bind     2485..2504
                     /label=oLZ048 NSP3-F
     primer_bind     2485..2500
                     /label=oYK28-mCherry-NS3a-F
     CDS             2485..2490
                     /label=EGFP
     primer_bind     complement(2486..2523)
                     /label=oLZ145 DNCR2-GNCR1 R
     primer_bind     2491..2534
                     /label=oYK472_NLS_F
     primer_bind     complement(2492..2505)
                     /label=Oscl18-4
     primer_bind     complement(2495..2523)
                     /label=oSCL075
     primer_bind     complement(2495..2523)
                     /label=oSCL075a
     primer_bind     complement(2495..2523)
                     /label=oSCL075`b
     primer_bind     complement(2495..2505)
                     /label=oSCL1119
     primer_bind     2504..2516
                     /label=oSCL1385
     CDS             2506..2553
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     2506..2535
                     /label=oSCL1385
     primer_bind     2512..2570
                     /label=oSCL1384
     primer_bind     2545..2609
                     /label=oYK419_GS_F
     primer_bind     complement(2565..2612)
                     /label=oYK438_SV40_R
     primer_bind     2567..2612
                     /label=oSCL1129
     primer_bind     2567..2610
                     /label=oLZ147 NS3a F
     primer_bind     2567..2609
                     /label=oYF0033
     primer_bind     complement(2571..2598)
                     /label=oSCL1614
     CDS             2572..2592
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2593..2622
                     /label=linker
     primer_bind     2593..2622
                     /label=oDJ171-3
     primer_bind     2593..2622
                     /label=oSCL388
     primer_bind     complement(2593..2618)
                     /label=oDJ171-4
     misc_feature    2593..2613
                     /label=linker
     primer_bind     2593..2613
                     /label=oYK451_NLS_F
     primer_bind     2593..2612
                     /label=oLZ048 NSP3-F
     primer_bind     2593..2608
                     /label=oYK28-mCherry-NS3a-F
     CDS             2593..2598
                     /label=EGFP
     primer_bind     complement(2594..2622)
                     /label=oLZ145 DNCR2-GNCR1 R
     primer_bind     complement(2600..2618)
                     /label=Oscl18-4
     primer_bind     complement(2603..2622)
                     /label=oSCL075
     primer_bind     complement(2603..2622)
                     /label=oSCL075a
     primer_bind     complement(2603..2622)
                     /label=oSCL075`b
     primer_bind     complement(2603..2622)
                     /label=oSCL1119
     primer_bind     complement(2606..2648)
                     /label=oYK472_NLS_R
     primer_bind     2607..2622
                     /label=oLZ146 GNCR1-N F
     primer_bind     complement(2607..2622)
                     /label=oYK92_L7Ae_R
     primer_bind     complement(2609..2622)
                     /label=oYK489_GNCR1_R
     primer_bind     complement(2611..2622)
                     /label=oYK75_NLS_mcp_R
     primer_bind     complement(2622..2646)
                     /label=oYK431_NES2_R
     primer_bind     complement(2626..2648)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(2649..2666)
                     /label=oXQ161
     terminator      2722..2949
                     /label=bGH PA
     misc_feature    2995..3423
                     /label=f1 origin
     misc_feature    3428..3771
                     /label=SV40 early promoter and origin
     misc_feature    3617..3694
                     /label=SV40 ORI
     CDS             3836..4627
                     /label=Kan/neoR
     misc_feature    4801..4931
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(4967..4987)
                     /label=M13-rev
     misc_binding    complement(4993..5015)
                     /label=LacO
     promoter        complement(5020..5049)
                     /label=lac
     misc_feature    complement(5314..5987)
                     /label=pUC origin
     CDS             complement(6135..6794)
                     /label=AmpR
     promoter        complement(7034..7062)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gctggctagg ccaccatgga catcgagaag ctgtgcaaga aggcggaaga ggaggccaag
      961 gaggcccagg agaaggccga cgagctgaga caacgacacc ccgattcgca ggcggcggaa
     1021 gacgcggagg atctcgcaaa tctggctgtc gccgccgtgc tgaccgcttg tctgctggcc
     1081 caggagcatc ccaacgccga tatcgctaag ctgtgcatca aggccgcgag cgaagctgcg
     1141 gaggccgcgt caaaagccgc cgagctggcc caacggcacc cagacagtca agcagccaga
     1201 gatgctatca aactcgcctc tcaagccgcc cgcgccgtga tcctggccat catgctcgcc
     1261 gcagagaatc ccaacgctga tattgcaaag ctatgtatta aggcggccag cgaagccgca
     1321 gaggctgcct cgaaagctgc ggaacttgca cagcggcacc ccgactccca ggccgcccga
     1381 gatgcaatta aactggccag tcaggccgct gaggcggtgg agagagccat ctggctggcc
     1441 gccgagaacc ccaatgccga tatcgccaaa aaatgcatca aagcggcctc cgaagcagcc
     1501 gaggaggcct ctaaagctgc cgaagaggct caaagacacc cagacagcca gaaggcacgc
     1561 gacgagatca aggaggcctc tcagaaggcc gaggaggtga aggagagatg caagagcgcc
     1621 agcggaagtg gcggaggagg cgacgtcgct agcatgctca agatggagat ggtgagcaag
     1681 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1741 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1801 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1861 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1921 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1981 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     2041 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     2101 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     2161 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     2221 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     2281 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     2341 cgccactcca ccggcggcat ggacgagctg tacaagggaa gcggcggagc caccatgaaa
     2401 aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagctgga cggcagcgga
     2461 agccccaaga agaagaggaa ggtagccagc ggaagtggcg gaggaaaaag gccggcggcc
     2521 acgaaaaagg ccggccaggc aaaaaagaaa aagctggacg gcagcggaag ccccaagaag
     2581 aagaggaagg tagccagcgg aagtggcgga ggaggcgacg tctaaggatc cactagtcca
     2641 gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg
     2701 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     2761 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     2821 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     2881 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     2941 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     3001 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3061 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3121 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3181 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3241 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3301 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3361 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3421 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3481 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3601 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3661 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     3721 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     3781 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     3841 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     3901 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     3961 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     4021 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4081 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4141 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4201 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4261 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4321 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     4381 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4441 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4501 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4561 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4621 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4681 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     4741 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     4801 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4861 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4921 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     4981 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5041 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5101 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5161 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5221 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6421 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6721 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//