| 质粒名称: | pTXB1-Tn5 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60240 |
| 存储实验室: | Rickard Sandberg |
|
载体骨架基本信息 |
|
| 载体名称: | pTXB1 |
| 载体出品公司: | New England Biolabs |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6706 bp |
| 完整质粒大小: | 8079 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | tnpA from pBam1,Tn5 transposase |
| 物种来源: | E. coli |
| 插入大小: | 1428 bp |
| 基因突变: | E54K, L372P |
| GenBank编号 | HQ908071.1 |
| 启动子 | T7 promoter |
| 标签/融合蛋白: | Mxe intein - Chitin-binding domain（插入端的C末端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Xba(未破坏) |
| 3' 克隆位点: | Lgu1/SapI）（克隆过程中被破坏） |
| 5' 测序引物 | T7 promoter primer |
| 3' 测序引物 | GATTGCCATGCCGGTCAAGG |
|
文献引用方法 |
|
| 材料和方法部分: | pTXB1-Tn5 was a gift from Rickard Sandberg (Addgene plasmid # 60240 ; http://n2t.net/addgene:60240 ; RRID:Addgene_60240) |
| 参考文献部分: | Tn5 transposase and tagmentation procedures for massively-scaled sequencing projects. Picelli S, Bjorklund AK, Reinius B, Sagasser S, Winberg G, Sandberg R. Genome Res. 2014 Jul 30. pii: gr.177881.114. 10.1101/gr.177881.114 PubMed 25079858 |
买家导航
pTXB1-Tn5载体 pTXB1Tn5质粒基本信息
pTXB1-Tn5质粒图谱 pTXB1Tn5载体图谱和pTXB1-Tn5载体序列 pTXB1Tn5质粒序列多克隆位点信息
pTXB1-Tn5质粒 pTXB1Tn5载体简介
pTXB1-Tn5质粒序列 pTXB1Tn5载体序列
LOCUS pTXB1-Tn5 8082 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and
Chitin-binding domain..
ACCESSION .
VERSION .
KEYWORDS pTXB1-Tn5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8082)
AUTHORS Picelli S, Bjorklund AK, Reinius B, Sagasser S, Winberg G, Sandberg
R
TITLE Tn5 transposase and tagmentation procedures for massively-scaled
sequencing projects.
JOURNAL Genome Res. 2014 Jul 30. pii: gr.177881.114.
PUBMED 25079858
REFERENCE 2 (bases 1 to 8082)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8082
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 126..148
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(142..333)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(890..1972)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 1934..1953
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(1973..2050)
/gene="lacI"
/label=lacI promoter
terminator 2203..2289
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2386..2472
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2569..2655
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2752..2838
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2935..3021
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 3156..3175
/label=T7
/note="T7 promoter, forward primer"
promoter 3156..3174
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 3175..3199
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 3243..4670
/codon_start=1
/product="transposase from the bacterial Tn5 transposon
(Reznikoff, 1993)"
/label=Tn5 transposase
/translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
KLDGFLAAKDLMAQGIKI"
CDS 4671..5264
/codon_start=1
/gene="Mycobacterium xenopi gyrA"
/product="modified GyrA intein (Southworth et al., 1999)"
/label=Mxe GyrA intein
/note="N-terminal cleavage of this intein can be induced by
thiols."
/translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
CDS 5295..5450
/codon_start=1
/gene="Bacillus circulans chiA"
/product="chitin binding domain from chitinase A1 (Watanabe
et al., 1994)"
/label=CBD
/translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
PALWQLQ"
primer_bind complement(5515..5533)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 5529..5576
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 5636..5740
/gene="bla"
/label=AmpR promoter
CDS 5741..6601
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5959..5978)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin complement(6643..7156)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6838..6859)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7050..7069
/label=F1ori-R
/note="F1 origin, reverse primer"
rep_origin 7267..7855
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7756..7775
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8009..8026
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(8048..8067)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
61 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
121 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
181 gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc
241 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
301 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
361 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
421 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
481 gcgggaccag agaaaaatca ctcagggtca atgccagccg aacgccagca agacgtagcc
541 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
601 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
661 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
721 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
781 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
841 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc
901 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
961 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca
1021 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
1081 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
1141 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
1201 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
1261 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
1321 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
1381 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
1441 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
1501 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
1561 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
1621 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
1681 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
1741 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
1801 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
1861 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
1921 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
1981 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
2041 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc
2101 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg ccggcatgcc
2161 gccctttcgt cttcaagaat taattcccaa ttccccaggc atcaaataaa acgaaaggct
2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
2281 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
2341 gcaggacgcc cgccataaac tgccaggaat taattcccca ggcatcaaat aaaacgaaag
2401 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
2461 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
2521 cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga
2581 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
2641 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
2701 tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa
2761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
2821 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
2881 gggtggcggg caggacgccc gccataaact gccaggaatt aattccccag gcatcaaata
2941 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
3001 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
3061 ggagggtggc gggcaggacg cccgccataa actgccagga attggggatc ggaattaatt
3121 cccggtttaa accggggatc tcgatcccgc gaaattaata cgactcacta taggggaatt
3181 gtgagcggat aacaattccc ctctagaaat aattttgtta actttaagaa ggagatatac
3241 atatgattac cagtgcactg catcgtgcgg cggattgggc gaaaagcgtg ttttctagtg
3301 ctgcgctggg tgatccgcgt cgtaccgcgc gtctggtgaa tgttgcggcg caactggcca
3361 aatatagcgg caaaagcatt accattagca gcgaaggcag caaagccatg caggaaggcg
3421 cgtatcgttt tattcgtaat ccgaacgtga gcgcggaagc gattcgtaaa gcgggtgcca
3481 tgcagaccgt gaaactggcc caggaatttc cggaactgct ggcaattgaa gataccacct
3541 ctctgagcta tcgtcatcag gtggcggaag aactgggcaa actgggtagc attcaggata
3601 aaagccgtgg ttggtgggtg catagcgtgc tgctgctgga agcgaccacc tttcgtaccg
3661 tgggcctgct gcatcaagaa tggtggatgc gtccggatga tccggcggat gcggatgaaa
3721 aagaaagcgg caaatggctg gccgctgctg caacttcgcg tctgagaatg ggcagcatga
3781 tgagcaacgt gattgcggtg tgcgatcgtg aagcggatat tcatgcgtat ctgcaagata
3841 aactggccca taacgaacgt tttgtggtgc gtagcaaaca tccgcgtaaa gatgtggaaa
3901 gcggcctgta tctgtatgat cacctgaaaa accagccgga actgggcggc tatcagatta
3961 gcattccgca gaaaggcgtg gtggataaac gtggcaaacg taaaaaccgt ccggcgcgta
4021 aagcgagcct gagcctgcgt agcggccgta ttaccctgaa acagggcaac attaccctga
4081 acgcggtgct ggccgaagaa attaatccgc cgaaaggcga aaccccgctg aaatggctgc
4141 tgctgaccag cgagccggtg gaaagtctgg cccaagcgct gcgtgtgatt gatatttata
4201 cccatcgttg gcgcattgaa gaatttcaca aagcgtggaa aacgggtgcg ggtgcggaac
4261 gtcagcgtat ggaagaaccg gataacctgg aacgtatggt gagcattctg agctttgtgg
4321 cggtgcgtct gctgcaactg cgtgaatctt ttactccgcc gcaagcactg cgtgcgcagg
4381 gcctgctgaa agaagcggaa cacgttgaaa gccagagcgc ggaaaccgtg ctgaccccgg
4441 atgaatgcca actgctgggc tatctggata aaggcaaacg caaacgcaaa gaaaaagcgg
4501 gcagcctgca atgggcgtat atggcgattg cgcgtctggg cggctttatg gatagcaaac
4561 gtaccggcat tgcgagctgg ggtgcgctgt gggaaggttg ggaagcgctg caaagcaaac
4621 tggatggctt tctggccgcg aaagacctga tggcgcaggg cattaaaatc tgcatcacgg
4681 gagatgcact agttgcccta cccgagggcg agtcggtacg catcgccgac atcgtgccgg
4741 gtgcgcggcc caacagtgac aacgccatcg acctgaaagt ccttgaccgg catggcaatc
4801 ccgtgctcgc cgaccggctg ttccactccg gcgagcatcc ggtgtacacg gtgcgtacgg
4861 tcgaaggtct gcgtgtgacg ggcaccgcga accacccgtt gttgtgtttg gtcgacgtcg
4921 ccggggtgcc gaccctgctg tggaagctga tcgacgaaat caagccgggc gattacgcgg
4981 tgattcaacg cagcgcattc agcgtcgact gtgcaggttt tgcccgcggg aaacccgaat
5041 ttgcgcccac aacctacaca gtcggcgtcc ctggactggt gcgtttcttg gaagcacacc
5101 accgagaccc ggacgcccaa gctatcgccg acgagctgac cgacgggcgg ttctactacg
5161 cgaaagtcgc cagtgtcacc gacgccggcg tgcagccggt gtatagcctt cgtgtcgaca
5221 cggcagacca cgcgtttatc acgaacgggt tcgtcagcca cgctactggc ctcaccggtc
5281 tgaactcagg cctcacgaca aatcctggtg tatccgcttg gcaggtcaac acagcttata
5341 ctgcgggaca attggtcaca tataacggca agacgtataa atgtttgcag ccccacacct
5401 ccttggcagg atgggaacca tccaacgttc ctgccttgtg gcagcttcaa tgactgcagg
5461 aaggggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
5521 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
5581 aaggaggaac tatatccgga taactacgtc aggtggcact tttcggggaa atgtgcgcgg
5641 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
5701 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
5761 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
5821 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
5881 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt tcccaatgat
5941 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
6001 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
6061 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
6121 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
6181 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
6241 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
6301 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
6361 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
6421 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
6481 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
6541 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
6601 actgtcagac caagtttact catatatact ttagattgat ttaccccggt tgataatcag
6661 aaaagcccca aaaacaggaa gattgtataa gcaaatattt aaattgtaaa cgttaatatt
6721 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
6781 atcggcaaaa tcccttataa atcaaaagaa tagcccgaga tagggttgag tgttgttcca
6841 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
6901 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg
6961 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
7021 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
7081 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
7141 ccgctacagg gcgcgtaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
7201 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
7261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
7321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
7381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
7441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
7501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
7561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
7621 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
7681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
7741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
7801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
7861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
7921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
7981 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag ctatggtgca
8041 ctctcagtac aatctgctct gatgccgcat agttaagcca gt
//
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