| 质粒名称: | pAAV-hSyn-GRAB_ATP1.0mut |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000134 |
| 产品用途: | Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | GRAB_ATP1.0mut |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo
Wu Z., He K., Chen Y., Li H., Pan S., Li B., Liu T., Xi F., Deng F., Wang H., Du J., Jing M., Li Y.
Neuron 110, 770-782.e5 (2022)
|
买家导航
pAAV-hSyn-GRAB_ATP1.0mut载体质粒基本信息
pAAV-hSyn-GRAB_ATP1.0mut质粒图谱载体图谱和pAAV-hSyn-GRAB_ATP1.0mut载体序列质粒序列多克隆位点信息
pAAV-hSyn-GRAB_ATP1.0mut质粒载体简介
pAAV-hSyn-GRAB_ATP1.0mut质粒序列载体序列
LOCUS pAAV-hSyn-GRAB_ATP1.0mut 6467 bp DNA circular SYN 21-APR-2023
DEFINITION Express the ATP-insensitive control sensor GRAB_ATP1.0mut in
neurons.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6467)
TITLE An ultrasensitive GRAB sensor for detecting extracellular ATP in
vitro and in vivo
REFERENCE 2 (bases 1 to 6467)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 27, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6467
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 131..574
/label=hSyn
regulatory 581..590
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
sig_peptide 587..649
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 650..674
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 674..2569
/codon_start=1
/label=GRAB_ATP1.0mut
/translation="TAEVLWPAVPNGTDAAFLAGPGSSWGNSTVASTAAVSSSFKCALT
KTGFQFYYLPAVYILVFIIGFLGNSVAIWMFVFHMKPWSGISVYMFNLALADFLYVLTL
PALIFYYFNKTDWIFGDAMCKLQRFIFHVNLYGSILFLTCISAHRYSGVVYPLKSLGRL
KKKNAICISVLVWLIVVVAISPILFYSGTGVRKNKTITCYDTTSDEYLRSYFIYSMCTT
VAMFCVPLVLILGCYGLIVRALIYPAPAGPRDTDALDLEEGGNVYIKADKQKNGIKANF
HIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFV
TAAGITLGMDELYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
EGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERT
IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAARWRGRSI
YLVIIVLTVFAVSYIPFHVMKTMALRARLDFQTPAMCAFNDRVYATYQVTRGLASLNSC
VDPILYFLAGDTFRRRLSRATRKASRRSEANLQSKSEDMTLNILPEFKQNGDTSL"
primer_bind 1119..1139
/label=WKG133-F
primer_bind 1672..1693
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1799..1820)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2060..2079)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 2594..3182
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2647..2667)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3065..3076)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3214..3690
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3413..3432)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3730..3870
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3730..3859
/label=AAV2 ITR
rep_origin 3945..4400
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4032..4051)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4242..4263
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4417..4436)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4536..4558
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4596..4614)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4682..4786
/gene="bla"
/label=AmpR promoter
CDS 4787..5647
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5005..5024)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5818..6406
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6307..6326
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtatgagg cggggtgggg gtgcctacct gacgaccgac
181 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
241 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
301 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
361 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
421 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
481 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
541 ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggt gccaccatgg agacagacac
601 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgaca caagtttgta
661 caaaaaagtt ggcaccgccg aggtgctgtg gccggctgtc cccaacggga cggacgctgc
721 cttcctggcc ggtccgggtt cgtcctgggg gaacagcacg gtcgcctcca ctgccgccgt
781 ctcctcgtcg ttcaaatgcg ccttgaccaa gacgggcttc cagttttact acctgccggc
841 tgtctacatc ttggtattca tcatcggctt cctgggcaac agcgtggcca tctggatgtt
901 cgtcttccac atgaagccct ggagcggcat ctccgtgtac atgttcaatt tggctctggc
961 cgacttcttg tacgtgctga ctctgccagc cctgatcttc tactacttca ataaaacaga
1021 ctggatcttc ggggatgcca tgtgtaaact gcagaggttc atctttcatg tgaacctcta
1081 tggcagcatc ttgtttctga catgcatcag tgcccaccgg tacagcggtg tggtgtaccc
1141 cctcaagtcc ctgggccggc tcaaaaagaa gaatgcgatc tgtatcagcg tgctggtgtg
1201 gctcattgtg gtggtggcga tctcccccat cctcttctac tcaggtaccg gggtccgcaa
1261 aaacaaaacc atcacctgtt acgacaccac ctcagacgag tacctgcgaa gttatttcat
1321 ctacagcatg tgcacgaccg tggccatgtt ctgtgtcccc ttggtgctga ttctgggctg
1381 ttacggatta attgtgagag ctttgattta ccccgcgccg gccgggccgc gcgacaccga
1441 cgcgctggac ctggaggagg gaggaaacgt ctatatcaag gccgacaagc agaagaacgg
1501 catcaaggcg aacttccaca tccgccacaa catcgaggac ggcggcgtgc agctcgccta
1561 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
1621 cctgagcgtg cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct
1681 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg
1741 taccggaggg agcatggtga gaaagggcga ggagctgttc accggggtgg tgcccatcct
1801 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg
1861 cgatgccacc gagggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
1921 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
1981 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga
2041 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
2101 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
2161 catcctgggg cacaagctgg agtacaacac cggagcagca gcacgctggc gcgggcggtc
2221 gatttacctg gtaatcattg tactgactgt ttttgctgtg tcttacatcc ctttccatgt
2281 gatgaaaacg atggctttga gggcccggct tgattttcag accccagcaa tgtgtgcttt
2341 caatgacagg gtttatgcca cgtatcaggt gacaagaggt ctagcaagtc tcaacagttg
2401 tgtggacccc attctctatt tcttggcggg agatactttc agaaggagac tctcccgagc
2461 cacaaggaaa gcttctagaa gaagtgaggc aaatttgcaa tccaagagtg aagacatgac
2521 cctcaatatt ttacctgagt tcaagcagaa tggagataca agcctgtaag aattcgatat
2581 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
2641 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
2701 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
2761 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
2821 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
2881 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
2941 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
3001 tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
3061 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
3121 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
3181 gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca
3241 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
3301 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
3361 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
3421 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
3481 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
3541 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
3601 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
3661 gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg
3721 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
3781 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
3841 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
3901 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
3961 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
4021 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
4081 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4141 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4201 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
4261 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
4321 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
4381 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
4441 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
4501 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
4561 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
4621 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
4681 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
4741 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
4801 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
4861 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
4921 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
4981 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
5041 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
5101 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5161 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
5221 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
5281 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
5341 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
5401 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
5461 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
5521 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
5581 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
5641 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
5701 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
5761 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
5821 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
5881 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
5941 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
6001 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
6061 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6121 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6181 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
6241 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
6301 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
6361 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
6421 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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