pYK155

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

买家导航

pYK155载体质粒基本信息

质粒名称: pYK155
出品公司: Wekwikgene
目录编号: 0000794
产品用途: Mammalian PhCMV-driven constitutive HNRNPA1-AG-NS3a(H1) expression vector (PhCMV-HNRNPA1-AG-NS3a(H1)-pA).
存储实验室: Anping ZENG Lab

载体骨架基本信息
载体名称: pCMV
表达系统: 哺乳动物表达
启动子: CMV
拷贝数: 高拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: Stbl3

插入基因信息
插入基因名称: HNRNPA1-AG-NS3a(H1)
物种来源: Synthetic
密码子优化: 哺乳细胞表达优化

文献引用方法
参考文献部分:
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)

pYK155质粒图谱载体图谱和pYK155载体序列质粒序列多克隆位点信息

pYK155载体图谱

pYK155质粒载体简介

pYK155质粒序列载体序列

LOCUS      pYK155              7940 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     880..931
                     /label=oDJ173-1
     primer_bind     881..933
                     /label=oYK211_HNRNPA1_F
     primer_bind     881..921
                     /label=oYK78_HNRNPA1_F
     primer_bind     881..909
                     /label=oYK30-NS3a_Head-F
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     gene            895..1866
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /label=HNRNPA1
                     /note="heterogeneous nuclear ribonucleoprotein A1"
                     /db_xref="GeneID:3178"
                     /db_xref="HGNC:HGNC:5031"
                     /db_xref="MIM:164017"
     exon            895..921
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /inference="alignment:same species:2.1.0"
     primer_bind     901..923
                     /label=oCW33
     CDS             907..1866
                     /codon_start=1
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /product="heterogeneous nuclear ribonucleoprotein A1isoform
                     b"
                     /label=HNRNPA1
                     /note="isoform b is encoded by transcript variant 2; 
                     heterogeneous nuclear ribonucleoprotein B2 protein; nuclear
                     ribonucleoprotein particle A1 protein; helix-destabilizing 
                     protein; single-strand DNA-binding protein UP1; 
                     heterogeneous nuclear ribonucleoprotein core protein A1; 
                     heterogeneous nuclear ribonucleoprotein A1B protein; hnRNP 
                     core protein A1-like 3; single-strand RNA-binding protein; 
                     putative heterogeneous nuclear ribonucleoprotein A1-like 3;
                     epididymis secretory sperm binding protein"
                     /db_xref="CCDS:CCDS44909.1"
                     /db_xref="GeneID:3178"
                     /db_xref="HGNC:HGNC:5031"
                     /db_xref="MIM:164017"
                     /protein_id="NP_112420.1"
                     /translation="MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVV
                     MRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTV
                     KKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIV
                     IQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNF
                     SGRGGFGGSRGGGGYGGSGDGYNGFGNDGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNF
                     GGRSSGPYGGGGQYFAKPRNQGGYGGSSSSSSYGSGRRF"
     misc_feature    907..909
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="N-acetylmethionine. 
                     /evidence=ECO:0007744|PubMed:22814378; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); acetylation site"
     misc_feature    910..912
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:20068231,
                      ECO:0007744|PubMed:23186163; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
     misc_feature    910..912
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="N-acetylserine, in Heterogeneous nuclear 
                     ribonucleoprotein A1, N-terminally processed. 
                     /evidence=ECO:0000269|Ref.9, ECO:0007744|PubMed:20068231, 
                     ECO:0007744|PubMed:22814378; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); acetylation site"
     misc_feature    913..915
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:19608861; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); acetylation site"
     misc_feature    916..1188
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="propagated from UniProtKB/Swiss-Prot 
                     (P09651.5);Region: Globular A domain"
     misc_feature    916..918
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:20068231,
                      ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163; 
                     propagated from UniProtKB/Swiss-Prot (P09651.5); 
                     phosphorylation site"
     exon            922..1038
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    922..924
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:18669648,
                      ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, 
                     ECO:0007744|PubMed:23186163; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
     misc_feature    970..972
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine./evidence=ECO:0000250|UniProtKB:P49312
                     ; propagated from UniProtKB/Swiss-Prot (P09651.5); 
                     phosphorylation site"
     exon            1039..1185
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /inference="alignment:same species:2.1.0"
     exon            1186..1396
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1189..1461
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="propagated from UniProtKB/Swiss-Prot 
                     (P09651.5);Region: Globular B domain"
     exon            1397..1489
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1480..1482
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine, by MKNK2. 
                     /evidence=ECO:0000269|PubMed:16111636; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
     misc_feature    1486..1488
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Asymmetric dimethylarginine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P09867; Dimethylated 
                     arginine, alternate. /evidence=ECO:0007744|PubMed:15782174;
                     Omega-N-methylarginine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P49312; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); methylation site"
     exon            1490..1582
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1501..1503
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:21406692;
                      propagated from UniProtKB/Swiss-Prot (P09651.5); 
                     phosphorylation site"
     misc_feature    1522..1524
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Asymmetric dimethylarginine, alternate. 
                     /evidence=ECO:0000269|Ref.9, ECO:0007744|PubMed:24129315; 
                     Dimethylated arginine, alternate. 
                     /evidence=ECO:0007744|PubMed:15782174; 
                     Omega-N-methylarginine, alternate. 
                     /evidence=ECO:0000269|Ref.9, ECO:0007744|PubMed:24129315; 
                     propagated from UniProtKB/Swiss-Prot (P09651.5); 
                     methylation site"
     misc_feature    1558..1626
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="propagated from UniProtKB/Swiss-Prot 
                     (P09651.5);Region: RNA-binding RGG-box"
     misc_feature    1558..1560
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Asymmetric dimethylarginine, alternate. 
                     /evidence=ECO:0007744|PubMed:24129315; 
                     Omega-N-methylarginine, alternate. 
                     /evidence=ECO:0007744|PubMed:24129315; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); methylation site"
     misc_feature    1579..1581
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Asymmetric dimethylarginine, alternate. 
                     /evidence=ECO:0000269|Ref.9, ECO:0007744|PubMed:24129315; 
                     Dimethylated arginine, alternate. 
                     /evidence=ECO:0007744|PubMed:15782174; 
                     Omega-N-methylarginine, alternate. 
                     /evidence=ECO:0000269|Ref.9, ECO:0007744|PubMed:24129315; 
                     propagated from UniProtKB/Swiss-Prot (P09651.5); 
                     methylation site"
     exon            1583..1657
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1600..1602
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Asymmetric dimethylarginine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P04256; 
                     Omega-N-methylarginine, alternate. 
                     /evidence=ECO:0007744|PubMed:24129315; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); methylation site"
     exon            1659..1813
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1708..1821
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="propagated from UniProtKB/Swiss-Prot 
                     (P09651.5);Region: Nuclear targeting sequence (M9)"
     misc_feature    1756..1758
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Omega-N-methylarginine. 
                     /evidence=ECO:0007744|PubMed:24129315; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); methylation site"
     misc_feature    1759..1761
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:23186163;
                      propagated from UniProtKB/Swiss-Prot (P09651.5); 
                     phosphorylation site"
     misc_feature    1798..1800
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:19608861; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); acetylation site"
     misc_feature    1804..1806
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Omega-N-methylarginine. 
                     /evidence=ECO:0007744|PubMed:24129315; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); methylation site"
     exon            1814..1866
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1831..1833
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:23186163;
                      propagated from UniProtKB/Swiss-Prot (P09651.5); 
                     phosphorylation site"
     misc_feature    1834..1836
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine, by MKNK2. 
                     /evidence=ECO:0000269|PubMed:16111636; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
     misc_feature    1837..1839
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine, by MKNK2. 
                     /evidence=ECO:0000269|PubMed:16111636; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
     misc_feature    1840..1842
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine, by MKNK2. 
                     /evidence=ECO:0000269|PubMed:16111636, 
                     ECO:0007744|PubMed:23186163; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
     primer_bind     complement(1841..1883)
                     /label=oDJ173-2
     misc_feature    1843..1845
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:21406692,
                      ECO:0007744|PubMed:23186163; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); phosphorylation site"
     primer_bind     complement(1844..1886)
                     /label=oYK78_HNRNPA1_R
     primer_bind     complement(1851..1866)
                     /label=oCW34
     misc_feature    1852..1854
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:18669648,
                      ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163; 
                     propagated from UniProtKB/Swiss-Prot (P09651.5); 
                     phosphorylation site"
     misc_feature    1858..1860
                     /gene="HNRNPA1"
                     /gene_synonym="ALS19; ALS20; hnRNP A1; hnRNP-A1; 
                     HNRPA1;HNRPA1L3; IBMPFD3; UP 1"
                     /note="Omega-N-methylarginine. 
                     /evidence=ECO:0007744|PubMed:24129315; propagated from 
                     UniProtKB/Swiss-Prot (P09651.5); methylation site"
     misc_feature    1867..2094
                     /label=Long_Linker
     primer_bind     1867..1886
                     /label=oYK78_AG_ANR1_F
     primer_bind     1867..1884
                     /label=oYK69_AG_ANR1_F
     CDS             1867
                     /codon_start=1
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /product="RNA-binding protein FUS isoform 1"
                     /label=FUS
                     /note="isoform 1 is encoded by transcript variant 1; 
                     heterogeneous nuclear ribonucleoprotein P2; fused in 
                     sarcoma; fusion gene in myxoid liposarcoma; 75 kDa 
                     DNA-pairing protein; RNA-binding protein FUS; oncogene TLS;
                     fus-like protein; oncogene FUS; translocated in liposarcoma
                     protein"
                     /db_xref="CCDS:CCDS10707.1"
                     /db_xref="GeneID:2521"
                     /db_xref="HGNC:HGNC:4010"
                     /db_xref="MIM:137070"
                     /protein_id="NP_004951.1"
                     /translation=""
     misc_feature    2095..2769
                     /label=AG
     primer_bind     2543..2553
                     /label=oSCL1104
     primer_bind     complement(2757..2777)
                     /label=oYK65-AG-R
     misc_feature    2770..2820
                     /label=Short_Linker
     primer_bind     complement(2796..2820)
                     /label=oYK33-35-PB1-AG-R
     primer_bind     2801..2841
                     /label=oYK153_NS3a_F
     CDS             2821..3408
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     2821..2856
                     /label=oSCL1082
     primer_bind     2821..2841
                     /label=oyk03-03-F
     primer_bind     2821..2841
                     /label=oYK04-ns3a-F
     primer_bind     2821..2839
                     /label=oYK25-NS3a-F
     primer_bind     2821..2838
                     /label=oYK30-NS3a_Head-F
     primer_bind     2838..2896
                     /label=oSCL1083
     primer_bind     2877..2935
                     /label=oSCL1084
     primer_bind     2914..2972
                     /label=oSCL1085
     primer_bind     2951..3009
                     /label=oSCL1086
     primer_bind     complement(2979..2998)
                     /label=oSCL1106
     primer_bind     2980..3018
                     /label=oSCL1104
     primer_bind     complement(3215..3273)
                     /label=oSCL1105
     primer_bind     complement(3255..3326)
                     /label=oSCL1087
     primer_bind     3257..3278
                     /label=oSCL1107
     primer_bind     3308..3326
                     /label=oSCL1088
     primer_bind     complement(3390..3437)
                     /label=oSCL1089
     primer_bind     complement(3393..3440)
                     /label=oYK30-NS3a_head-R
     primer_bind     complement(3409..3457)
                     /label=oYK03-03-R
     primer_bind     complement(3409..3428)
                     /label=oSCL1130
     primer_bind     complement(3417..3437)
                     /label=olyl382
     primer_bind     complement(3438..3460)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(3438..3457)
                     /label=oYK25-NS3a-R
     primer_bind     complement(3461..3481)
                     /label=oCW34
     primer_bind     complement(3462..3481)
                     /label=oSCL1089
     terminator      3534..3761
                     /label=bGH PA
     misc_feature    3807..4235
                     /label=f1 origin
     misc_feature    4240..4583
                     /label=SV40 early promoter and origin
     misc_feature    4429..4506
                     /label=SV40 ORI
     CDS             4648..5439
                     /label=Kan/neoR
     misc_feature    5613..5743
                     /label=SV40 early polyadenylation signal
     primer_bind     5717..5736
                     /label=olyl382
     primer_bind     complement(5779..5799)
                     /label=M13-rev
     misc_binding    complement(5805..5827)
                     /label=LacO
     promoter        complement(5832..5861)
                     /label=lac
     primer_bind     5976..5987
                     /label=oYK69_AG_ANR1_F
     misc_feature    complement(6126..6799)
                     /label=pUC origin
     CDS             complement(6947..7606)
                     /label=AmpR
     primer_bind     7263..7274
                     /label=oyk03-03-F
     primer_bind     7263..7274
                     /label=oYK04-ns3a-F
     primer_bind     7263..7274
                     /label=oYK153_NS3a_F
     promoter        complement(7846..7874)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgt ctaagtcaga gtctcctaaa gagcccgaac agctgaggaa gctcttcatt
      961 ggagggttga gctttgaaac aactgatgag agcctgagga gccattttga gcaatgggga
     1021 acgctcacgg actgtgtggt aatgagagat ccaaacacca agcgctccag gggctttggg
     1081 tttgtcacat atgccactgt ggaggaggtg gatgcagcta tgaatgcaag gccacacaag
     1141 gtggatggaa gagttgtgga accaaagaga gctgtctcca gagaagattc tcaaagacca
     1201 ggtgcccact taactgtgaa aaagatattt gttggtggca ttaaagaaga cactgaagaa
     1261 catcacctaa gagattattt tgaacagtat ggaaaaattg aagtgattga aatcatgact
     1321 gaccgaggca gtggcaagaa aaggggcttt gcctttgtaa cctttgacga ccatgactcc
     1381 gtggataaga ttgtcattca gaaataccat actgtgaatg gccacaactg tgaagttaga
     1441 aaagccctgt caaagcaaga gatggctagt gcttcatcca gccaaagagg tcgaagtggt
     1501 tctggaaact ttggtggtgg tcgtggaggt ggtttcggtg ggaatgacaa cttcggtcgt
     1561 ggaggaaact tcagtggtcg tggtggcttt ggtggcagcc gtggtggtgg tggatatggt
     1621 ggcagtgggg atggctataa tggatttggt aatgatggaa gcaattttgg aggtggtgga
     1681 agctacaatg attttgggaa ttacaacaat cagtcttcaa attttggacc catgaaggga
     1741 ggaaattttg gaggcagaag ctctggcccc tatggcggtg gaggccaata ctttgcaaaa
     1801 ccacgaaacc aaggtggcta tggcggttcc agcagcagca gtagctatgg cagtggcaga
     1861 agatttggtt ctgggagtgg cggtagtggt gctggaggca gcgcaggctc tggagcaggc
     1921 ggcagtgccg gaagtggcgc tggagggtct gccggatctg gagccggtgg ttctgccggc
     1981 tccggcgctg gtgggagcgc ttccggaagt agtcccggta attccgctga cggcggcgga
     2041 ggatcgggtg gtagtggtgg ttcaggagga ggatcgaccc aaggaggatc catggtgagc
     2101 gtgatcaagc ccgagatgaa gatcaagctg tgcatgaggg gcaccgtgaa cggccacaac
     2161 ttcgtgatcg agggcgaggg caagggcaac ccctacgagg gcacccagat cctggacctg
     2221 aacgtgaccg agggcgcccc cctgcccttc gcctacgaca tcctgaccac cgtgttccag
     2281 tacggcaaca gggccttcac caagtacccc gccgacatcc aggactactt caagcagacc
     2341 ttccccgagg gctaccactg ggagaggagc atgacctacg aggaccaggg catctgcacc
     2401 gccaccagca acatcagcat gaggggcgac tgcttcttct acgacatcag gttcgacggc
     2461 gtgaacttcc cccccaacgg ccccgtgatg cagaagaaga ccctgaagtg ggagcccagc
     2521 accgagaaga tgtacgtgag ggacggcgtg ctgaagggcg acgtgaacat ggccctgctg
     2581 ctggagggcg gcggccacta caggtgcgac ttcaagacca cctacaaggc caagaaggac
     2641 gtgaggctgc ccgactacca cttcgtggac cacaggatcg agatcctgaa gcacgacaag
     2701 gactacaaca aggtgaagct gtacgagaac gccgtggcca ggtacagcat gctgcccagc
     2761 caggccaagg gtaccggaac tgcagcagag aattcgggaa actcgagaac aaagcttgaa
     2821 aagaagaagg gcagcgtggt gatcgtgggc agaatcaacc tgagcggcga caccgcctac
     2881 agccagcaga ccagaggcct ggagggctgc caggagacca gccagaccgg cagagacaag
     2941 aaccaggtgg agggcgaggt gcaggtggtg agcaccgcca cccagagctt cctggccacc
     3001 agcatcaacg gcgtgctgtg gaccgtgtac cacggcgccg gcaccagaac catcgccagc
     3061 cccaagggcc ccgtgaccca gatgtacacg aacgtggaca aggacttagt gggctggcag
     3121 gccccccaag gcagcagaag cctgaccccc tgcacctgcg gcagcagcga cctgtacctg
     3181 gtgaccagac acgccgacgt gatccccgtg agaggaagag gcgacagcag aggcagcctg
     3241 ctgagcccca gacccatcag ctatctgaaa ggctcaagcg gcggccccct gttgtgtccc
     3301 gccggccatg ccgtgggcat cttcagagcc gccgtgagca ccagaggcgt ggccaaggcc
     3361 gtggacttca tccccgtgga gagcctggag accaccatga gaagccccgc tagctagaaa
     3421 ttctgcagat atccagcgga tccactagtc cagtgtggtg gaattctgca gatatccagc
     3481 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     3541 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3601 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3661 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3721 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3781 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     3841 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3901 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3961 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4021 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4081 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4141 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4201 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     4261 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4321 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4381 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4441 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4501 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4561 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     4621 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     4681 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4741 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4801 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     4861 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4921 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4981 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5041 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5101 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5161 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     5221 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     5281 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     5341 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     5401 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     5461 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     5521 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5581 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5641 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5701 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     5761 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5821 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5881 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5941 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6001 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6061 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6181 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7081 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7921 gaaaagtgcc acctgacgtc
//

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