| 质粒名称: | pET-hisPNK |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002245 |
| 产品用途: | active T4 Polynucleotide Kinase |
| 存储实验室: | Po-Min Chiang Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pET3c |
| 表达系统: | 细菌表达 |
| 启动子: | T7 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Neb stable |
|
插入基因信息 |
|
| 插入基因名称: | T4 PNK |
| 物种来源: |
T4 phage
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
USPPAR is a cost-effective, scalable, and highly sensitive single-cell RNA sequencing workflow compatible with diverse specimens.
Hsueh, Y.-W. & Chiang, P.-M.
PLOS Biol. 23, e3003537 (2025)
|
买家导航
pET-hisPNK载体质粒基本信息
pET-hisPNK质粒图谱载体图谱和pET-hisPNK载体序列质粒序列多克隆位点信息
pET-hisPNK质粒载体简介
pET-hisPNK质粒序列载体序列
LOCUS pET-hisPNK 5570 bp DNA circular SYN 17-APR-2026
DEFINITION Expresses his-tagged PNK in E. coli hosts.
ACCESSION .
VERSION .
KEYWORDS pET-hisPNK
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5570)
TITLE Bacterial expression plasmids for production of recombinant PNK
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5570)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..5570
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 10..38
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
terminator complement(404..451)
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind 447..465
/label=T7 Term
/note="T7 terminator, reverse primer"
CDS complement(1425..1436)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS complement(1449..1478)
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
RBS 1492..1514
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
primer_bind complement(1545..1564)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(1546..1564)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1648..1667)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS 3125..3316
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind complement(3310..3332)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3418..3557
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 3473..3492
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(3572..3589)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3743..4331)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3823..3842)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4502..5362)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5125..5144
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5363..5467)
/gene="bla"
/label=AmpR promoter
primer_bind 5535..5553
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccttaaa aatctcccga agcgacttgc
541 cagcattcaa caccgatacg acgccacatt tcaactactt gagttcggtc atcaatagct
601 aatttcacgt caaaatgcgg tgcaatgtgt ttccagaaaa tttcttcttt aactacatcg
661 tctttacggg tatcgccttg ttcgcgctga cattgcataa ctaatggaac gccagcaatg
721 tcctcaaccc atttacgggt catacgataa tatttcgttg ggtcttcttt agttccactt
781 tcacgacctg aaacgactac gatttgataa cccataagag catacatctt agacagttca
841 acaaccatag gattgataac atcggtatcg catttttcaa ggtcataagg accacgacca
901 ttcattttag ctagtgtacc atcaacatca aaaataactg cttttggttt accaggagtc
961 ccattatata ctggaagacc gagatactct cgcatgcttt tatacattga acgtaaaaca
1021 tcaattggta ctgctttagt tccgcgtttt gagttacgtt taaccaattc agtccaagga
1081 acatcaaaca ctttatgttc aactttccag ccgtattctt tggcaaaagt ttcccatgct
1141 aggcgacgtt caggattcag gttagtatct gaaatgatta ctcccttaac agaatcgcca
1201 ccgtacagaa tacttttagc tgtatcaaac tgcataccag ttacgatacc ttctttcttt
1261 ttggtatact tgtactcatc gcgttcttca tgcgccataa tagattggcg atagtcatca
1321 cgattgatat tataaaaccc gggattctta gcaataaatt cacgagccca agtactctta
1381 ccagaaccag gacagccaat agtcaaaata atctttttca tatgacgacc ttcgatatgg
1441 ccgctgctgt gatgatgatg atgatgatga tgatgatggc ccatatgtat atctccttct
1501 taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat agtgagtcgt
1561 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
1621 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
1681 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
1741 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
1801 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
1861 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
1921 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
1981 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
2041 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
2101 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
2161 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
2221 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
2281 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
2341 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
2401 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
2461 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
2521 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
2581 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
2641 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
2701 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
2761 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
2821 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
2881 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
2941 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
3001 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
3061 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc
3121 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
3181 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga
3241 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga
3301 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
3361 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
3421 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
3481 gagcagattg tactgagagt gcaccattgc ggtgtgaaat accgcacaga tgcgtaagga
3541 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
3601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
3661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
3721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
3781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
3841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
3901 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
3961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
4021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
4081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
4141 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
4201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
4261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
4321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
4381 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
4441 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
4501 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
4561 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
4621 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
4681 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
4741 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
4801 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
4861 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
4921 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
4981 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
5041 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
5101 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
5161 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
5221 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
5281 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
5341 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
5401 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
5461 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
5521 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
//
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