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pcDNA3.1+/C-Myc载体质粒基本信息
pcDNA3.1+/C-Myc质粒图谱载体图谱和pcDNA3.1+/C-Myc载体序列质粒序列多克隆位点信息
pcDNA3.1+/C-Myc质粒载体简介
pcDNA3.1+/C-Myc质粒序列载体序列
LOCUS pcDNA3.1+ C-Myc 5444 bp ds-DNA circular SYN 21-MAY-2015
DEFINITION Mammalian expression vector with a CMV promoter for expressing
C-terminally Myc-tagged proteins.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1+ C-Myc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5444)
AUTHORS GenScript
TITLE Direct Submission
JOURNAL Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5444
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 895..985
/note="MCS"
/note="multiple cloning site"
CDS 986..1015
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
polyA_signal 1041..1265
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1311..1739
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1753..2082
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1933..2068
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2149..2943
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3117..3238
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3689..4277)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4448..5308)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5309..5413)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccgaattc tgcagatatc cagcacagtg
961 gcggccgctc gagtctagag ggcccgagca gaaactcatc tctgaagagg atctgtgata
1021 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
1081 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
1141 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
1201 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
1261 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
1321 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
1381 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
1441 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
1501 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
1561 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
1621 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
1681 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
1741 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
1801 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
1861 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
1921 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
1981 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
2041 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
2101 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
2161 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
2221 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
2281 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
2341 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
2401 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
2461 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
2521 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
2581 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
2641 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
2701 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
2761 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
2821 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
2881 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
2941 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
3001 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
3061 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
3121 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
3181 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
3241 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
3301 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
3361 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
3421 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
3481 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
3541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
3601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
3661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
3721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
3781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
3841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
3901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
3961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
4021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
4081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
4141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
4201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
4261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
4321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
4381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
4441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
4501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
4561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
4621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
4681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
4741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
4801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
4861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
4921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
4981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
5041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
5101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
5161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
5221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
5281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
5341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
5401 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
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