| 质粒名称: | pSec61beta-mBaoJin |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001820 |
| 产品用途: | Express mBaoJin fused to endoplasmic reticulum in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pSec61beta |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Top10 |
|
插入基因信息 |
|
| 插入基因名称: | mBaoJin |
| 物种来源: | mammalian |
| 密码子优化: | 未优化 |
pSec61beta-mBaoJin
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSec61beta-mBaoJin载体质粒基本信息
pSec61beta-mBaoJin质粒图谱载体图谱和pSec61beta-mBaoJin载体序列质粒序列多克隆位点信息
pSec61beta-mBaoJin质粒载体简介
pSec61beta-mBaoJin质粒序列载体序列
LOCUS pSec61beta-mBaoJin 4939 bp DNA circular SYN 02-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS 1820-pSec61beta-mBaoJin
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4939)
AUTHORS Jingze Lu
TITLE Direct Submission
JOURNAL Exported Aug 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4939
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(565..596)
/label=Sec61-V-R
primer_bind 574..614
/label=BJ-Sec61-F
primer_bind 596..618
/label=0709-EB3-fmStayG-F
CDS 597..1298
/codon_start=1
/label=mBaoJin
/translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
EAHLKGMDELYK"
primer_bind complement(1277..1298)
/label=0709-EB3-fmStayG-R
primer_bind complement(1281..1315)
/label=BJ-Sec61-R
primer_bind 1294..1317
/label=Sec61-V-F
CDS 1314..1604
/codon_start=1
/label=sec61b
/translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
misc_feature 1606..1613
/label=Not1
polyA_signal 1727..1848
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1855..2310)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2337..2441
/gene="bla"
/label=AmpR promoter
promoter 2443..2800
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2651..2786
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2835..3629
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3861..3908
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4237..4825
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
601 tctcaaaggg agaggaggaa aacatggcta gtacaccatt taaatttcaa cttaaaggaa
661 ccatcaatgg caaatcgttt accgttgaag gcgaaggtga agggaactca catgaaggtt
721 ctcataaagg aaaatatgtt tgtacaagtg gaaaactacc gatgtcatgg gcagcacttg
781 ggacaacctt tggttatgga atgaaatatt ataccaaata tcctagtgga ctgaagaact
841 ggtttcgtga agtaatgccc ggaggcttta cctacgatcg tcatattcaa tataaaggcg
901 atgggagtat ccatgcaaaa caccaacact ttatgaaaaa tgggacttat cacaacattg
961 tagaatttac cggtcaggat tttaaagaaa atagtccagt cttaactgga gatatgaatg
1021 tctcattacc gaatgaagtc ccacaaatac ccagagatga tggagtagaa tgcccagtga
1081 ccttgcttta tcctttatta tcggataaat caaaatacgt cgaggctcac caatatacaa
1141 tctgcaagcc tcttcataat caaccagcac ctgatgtccc atatcactgg attcgtaaac
1201 aatacacaca aagcaaagat gatgccgagg aacgcgatca tatctgtcaa tcagagactc
1261 tcgaagcaca cttaaagggc atggacgagc tgtataagcc accggtcgcc accatgcctg
1321 gtccgacccc cagtggcact aacgtgggat cctcagggcg ctctcccagc aaagcagtgg
1381 ccgcccgggc ggcgggatcc actgtccggc agaggaaaaa tgccagctgt gggacaagga
1441 gtgcaggccg cacaacctcg gcaggcaccg gggggatgtg gcgattctac acagaagatt
1501 cacctgggct caaagttggc cctgttccag tattggttat gagtcttctg ttcatcgctt
1561 ctgtatttat gttgcacatt tggggcaagt acactcgttc gtagagcggc cgcgactcta
1621 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
1681 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
1741 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
1801 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
1861 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
1921 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
1981 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
2041 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
2101 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
2161 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
2221 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
2281 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
2341 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
2401 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
2461 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
2521 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
2581 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
2641 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
2701 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
2761 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
2821 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
2881 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
2941 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
3001 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
3061 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
3121 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
3181 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
3241 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
3301 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
3361 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
3421 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
3481 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
3541 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
3601 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
3661 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
3721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
3781 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
3841 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
3901 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
3961 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
4021 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
4081 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
4141 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
4201 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
4261 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
4321 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
4381 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
4441 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
4501 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
4561 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
4621 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
4681 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
4741 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
4801 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
4861 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
4921 ccgtattacc gccatgcat
//
