| 质粒名称: | pDJ687 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001160 |
| 产品用途: | Mammalian NFκB-responsive SEAP expression vector |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | NFkB-responsive |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NanoLuc |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Fluorescent protein-based ticker tapes for multiplexed recordings of transcriptional histories in single cells in culture and in vivo
Wang R., Jiang J., Li Z., Liu T., Wang Y., Xie M., Piatkevich K.D.
Preprint
|
pDJ687
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDJ687载体质粒基本信息
pDJ687质粒图谱载体图谱和pDJ687载体序列质粒序列多克隆位点信息
pDJ687质粒载体简介
pDJ687质粒序列载体序列
LOCUS pDJ687 6439 bp DNA circular SYN 10-MAR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6439)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Aug 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6439
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 78..99
/label=oYW269
/note="LA-NheI-F"
misc_feature 196..226
/label=SleepingBeauty-ITR_Lo
/label=nonstandard type: ITR
misc_feature 392..423
/label=SleepingBeauty-ITR_Li
/label=nonstandard type: ITR
primer_bind 451..498
/label=oYW336
primer_bind 460..476
/label=oYW181
/note="P_hCMV-F"
primer_bind complement(460..476)
/label=oYW179
/note="ITR PCR F"
misc_feature 477..1580
/label=EF-1a
/note="/vntifkey=21"
primer_bind 477..498
/label=oYW338
/note="LA-NruI-PEF1a PCR-F"
promoter 493..1671
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
primer_bind 496..510
/label=oYW43
/note="LA-MluI-hEF1a PCR F"
primer_bind 496..510
/label=oYW183
/note="P_hEF1a PCR F"
primer_bind 604..627
/label=oDJ223-1
primer_bind 605..627
/label=oYW355
/note="LA-NruI-PEF1a PCR-F"
primer_bind 605..624
/label=oDJ8-1
primer_bind complement(611..630)
/label=EF1a-R
primer_bind complement(671..690)
/label=oYW45
/note="hEF1a mutant TOP motif LA-R"
primer_bind 673..713
/label=oYW46
/note="hEF1a mutant TOP motif RA-F"
intron 724..1662
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 937..956
/label=oYW361
/note="PEF1a mid seq primer"
primer_bind 1076..1093
/label=oYW47
/note="hEF1a-Seq-F"
primer_bind 1438..1459
/label=oYW285
/note="PEF1a-F for pYW136/137"
primer_bind complement(1551..1560)
/label=oYW346
primer_bind 1619..1639
/label=pEF-F/EF-1aFwd
primer_bind complement(1640..1659)
/label=oYW184
/note="P_hEF1a PCR R"
primer_bind complement(1643..1697)
/label=oYW337
primer_bind complement(1643..1672)
/label=oYW348
/note="RA-PEF1a PCR R"
primer_bind complement(1643..1672)
/label=oYW354
/note="RA-PEF1a PCR R"
primer_bind complement(1643..1671)
/label=oYW339
/note="RA-NheI-PEF1a PCR R"
primer_bind 1643..1659
/label=oYW180
/note="Puro PCR R"
primer_bind 1670..1707
/label=oYW333
/note="LA-AgeI-PacI-EcoRV-IRES PCR-F"
misc_feature 1730..2303
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1776..1795)
/label=pIRES2-EGFP.P3
primer_bind complement(1825..1844)
/label=IRES-R
primer_bind 2301..2312
/label=oDJ199-3
primer_bind complement(2316..2361)
/label=oYW335
/note="RA-SalI-IRES PCR R"
primer_bind complement(2316..2350)
/label=oYW344
primer_bind complement(2316..2350)
/label=oYW345
primer_bind complement(2316..2335)
/label=oYW334
/note="IRES PCR R"
primer_bind 2336..2373
/label=oYW193
/note="LA-PuroR-F"
primer_bind 2351..2373
/label=oYW180
/note="Puro PCR R"
CDS 2353..2952
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2838..2891)
/label=oYS113
primer_bind complement(2937..2952)
/label=oYW194
/note="PuroR-R"
primer_bind complement(2995..3012)
/label=BGH/pcDNA3.1R
primer_bind complement(2995..3012)
/label=pcDNA3.1-R(BGH-R)
primer_bind 2998..3017
/label=oYW188
/note="bGH poly A"
polyA_signal 3001..3225
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(3005..3022)
/label=oYW190
/note="BGH poly A R"
primer_bind complement(3067..3085)
/label=oYW303
/note="PolyA tail-bGH pA"
primer_bind complement(3067..3085)
/label=oYW57
/note="bGH seq primer R"
primer_bind complement(3070..3097)
/label=oYW300
/note="PolyA tail-bGH pA"
primer_bind complement(3070..3093)
/label=oYW302
/note="bGH pA R"
primer_bind 3070..3089
/label=W2F0627-P099-D03
/note="Sequencing primer"
primer_bind 3128..3147
/label=BGH-polyA-R
primer_bind 3208..3254
/label=oYW203
/note="LA-P_CMV-F"
primer_bind complement(3208..3231)
/label=oYW202
/note="BGH polyA-R"
primer_bind complement(3208..3225)
/label=oYW56
/note="LA-BamHI-PPT-MluI-bGH pA PCR"
primer_bind complement(3208..3225)
/label=oYW53
/note="LA-BamHI-bGH pA"
primer_bind complement(3209..3226)
/label=oYW282
/note="Arm-PCR pYW134-R"
primer_bind 3226..3254
/label=oYW189
/note="LA-P_CMV-F"
primer_bind 3245..3262
/label=oYW90
/note="LA-AgeI-PBS-PCMV F"
primer_bind 3246..3271
/label=oYW355
/note="LA-NruI-PEF1a PCR-F"
primer_bind 3254..3270
/label=oYW326
primer_bind 3274..3316
/label=oDJ687-1
primer_bind 3274..3293
/label=oDJ223-1
primer_bind 3274..3292
/label=oYW278
/note="LA-NotI-P_GFAP-F"
primer_bind 3274..3292
/label=oYW181
/note="P_hCMV-F"
primer_bind 3294..3320
/label=oDJ656-5
primer_bind 3300..3320
/label=oDJ587-1
primer_bind 3301..3325
/label=oYW269
/note="LA-NheI-F"
misc_feature 3326..3335
/label=nfkb motif
misc_feature 3336..3345
/label=nfkb motif
misc_feature 3346..3355
/label=nfkb motif
misc_feature 3356..3365
/label=nfkb motif
promoter 3378..3409
/label=minP
/note="minimal TATA-box promoter with low basal activity"
misc_feature 3379..3409
/label=Minimal promoter
misc_feature 3385..3391
/label=TATATAA
primer_bind complement(3413..3434)
/label=oDJ587-2
primer_bind complement(3419..3454)
/label=oDJ687-2
primer_bind 3435..3467
/label=oQZ280
primer_bind 3435..3456
/label=oDJ684-3
primer_bind 3435..3452
/label=oQZ321
regulatory 3435..3443
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3441..3509
/codon_start=1
/label=Secretory (IgK)
/label=nonstandard type: signal
/translation="MTSETDTLLLWVLLLWVPGSTGD"
primer_bind 3441..3467
/label=oQZ331
CDS 3441..3443
/codon_start=1
/product="bright monomeric near-infrared fluorescent
protein engineered from a bacterial phytochrome, with an
emission maximum of 670 nm (Shcherbakova et al., 2016)"
/label=emiRFP670
/label=ORF frame 1
/translation="M"
primer_bind 3444..3465
/label=oQZ757
primer_bind complement(3511..3543)
/label=oQZ758
primer_bind 3511..3543
/label=oQZ759
CDS 3528..4043
/codon_start=1
/product="NanoLuc(R) luciferase"
/label=Nluc
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
primer_bind complement(3638..3661)
/label=oQZ826
primer_bind 3662..3686
/label=oQZ827
primer_bind complement(4022..4063)
/label=oDJ684-4
primer_bind complement(4022..4043)
/label=oQZ281
primer_bind complement(4022..4043)
/label=oDJ657-2
primer_bind complement(4025..4043)
/label=oQZ322
primer_bind complement(4063..4080)
/label=BGH/pcDNA3.1R
primer_bind complement(4063..4080)
/label=pcDNA3.1-R(BGH-R)
primer_bind 4066..4085
/label=oYW188
/note="bGH poly A"
polyA_signal 4069..4293
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(4073..4090)
/label=oYW190
/note="BGH poly A R"
primer_bind complement(4135..4153)
/label=oYW303
/note="PolyA tail-bGH pA"
primer_bind complement(4135..4153)
/label=oYW57
/note="bGH seq primer R"
primer_bind complement(4138..4165)
/label=oYW300
/note="PolyA tail-bGH pA"
primer_bind complement(4138..4161)
/label=oYW302
/note="bGH pA R"
primer_bind 4138..4157
/label=W2F0627-P099-D03
/note="Sequencing primer"
primer_bind 4196..4215
/label=BGH-polyA-R
primer_bind 4276..4294
/label=oYW203
/note="LA-P_CMV-F"
primer_bind complement(4276..4294)
/label=oYW202
/note="BGH polyA-R"
primer_bind complement(4276..4293)
/label=oYW56
/note="LA-BamHI-PPT-MluI-bGH pA PCR"
primer_bind complement(4276..4293)
/label=oYW53
/note="LA-BamHI-bGH pA"
primer_bind complement(4277..4294)
/label=oYW282
/note="Arm-PCR pYW134-R"
primer_bind 4304..4320
/label=oYW183
/note="P_hEF1a PCR F"
primer_bind 4305..4326
/label=oYW197
/note="ITR PCR F1"
primer_bind complement(4336..4351)
/label=oYW336
misc_feature complement(4377..4405)
/label=SleepingBeauty-ITR_Ri
/label=nonstandard type: ITR
misc_feature 4571..4602
/label=SleepingBeauty-ITR-Ro
/label=nonstandard type: ITR
primer_bind complement(4745..4764)
/label=oYW207
/note="pLL3.7 PCR R"
primer_bind complement(4746..4771)
/label=oYW301
/note="PolyA tail-pLL3.7 3'LTR"
rep_origin complement(4790..5378)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4929..4948)
/label=W5CF0703-P099-D09
/note="Sequencing primer"
CDS complement(5549..6409)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5566..5585)
/label=W4CF0701-P099-E08
/note="Sequencing primer"
primer_bind complement(6230..6249)
/label=W3CF0630-P099-D08
/note="Sequencing primer"
primer_bind complement(6286..6305)
/label=W3CF0630-P099-A08
/note="Sequencing primer"
primer_bind complement(6286..6305)
/label=W3CF0630-P099-F07
/note="Sequencing primer"
primer_bind 6380..6399
/label=oYW24
/note="Forward seq primer of AmpR"
promoter complement(join(6410..6439,1..75))
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
61 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc
121 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
181 attggatccc tatacagttg aagtcggaag tttacataca cttaagttgg agtcattaaa
241 actcgttttt caactactcc acaaatttct tgttaacaaa caatagtttt ggcaagtcag
301 ttaggacatc tactttgtgc atgacacaag tcatttttcc aacaattgtt tacagacaga
361 ttatttcact tataattcac tgtatcacaa ttccagtggg tcagaagttt acatacacta
421 agttcgactc ctctgcagaa tgcggcgatg tttcggtaag gggtccgcta gaattcgaaa
481 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
541 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
601 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
661 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
721 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
781 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
841 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
901 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
961 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
1021 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
1081 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
1141 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
1201 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
1261 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
1321 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
1381 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
1441 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
1501 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
1561 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
1621 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aaccggttta
1681 attaagatat ctgcatctag ggcggccaat tccgcccctc tcccccccgc ccctctccct
1741 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
1801 atatgtgatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
1861 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
1921 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
1981 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
2041 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
2101 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
2161 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
2221 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
2281 tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac cttccgtcga
2341 cgccaccatg ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
2401 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
2461 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
2521 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
2581 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
2641 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
2701 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
2761 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
2821 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
2881 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
2941 cccggtgcct gattcgaagg cctgtcgtgg gggatcaatt cgctcgctga tcagcctcga
3001 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
3061 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
3121 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
3181 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcctag gagaatctgc
3241 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtaaccagg
3301 gtaccgagct cttacgcgtg ctagcgggaa tttccgggaa tttccgggaa tttccgggaa
3361 tttccagatc tagactctag agggtatata atggaagctc gaattccagc ttggcattcc
3421 ggtactgttg gtaagccacc atgactagtg agacagacac actcctgcta tgggtactgc
3481 tgctctgggt tccaggttcc actggtgacg ctagtggtgg ttctggtatg gtcttcacac
3541 tcgaagattt cgttggggac tggcgacaga cagccggcta caacctggac caagtccttg
3601 aacagggagg tgtgtccagt ttgtttcaga atctcggggt gtccgtaact ccgatccaaa
3661 ggattgtcct gagcggtgaa aatgggctga agatcgacat ccatgtcatc atcccgtatg
3721 aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg gtgtaccctg
3781 tggatgatca tcactttaag gtgatcctgc actatggcac actggtaatc gacggggtta
3841 cgccgaacat gatcgactat ttcggacggc cgtatgaagg catcgccgtg ttcgacggca
3901 aaaagatcac tgtaacaggg accctgtgga acggcaacaa aattatcgac gagcgcctga
3961 tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc ggctggcggc
4021 tgtgcgaacg cattctggcg tgatctagag ctcgctgatc agcctcgact gtgccttcta
4081 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
4141 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
4201 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
4261 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
4321 cctactagct actcgggacc ccttaccgaa acatcgccgc attctgcaga ggagtcgagt
4381 gtatgtaaac ttctgaccca ctgggaatgt gatgaaagaa ataaaagctg aaatgaatca
4441 ttctctctac tattattctg atatttcaca ttcttaaaat aaagtggtga tcctaactga
4501 cctaagacag ggaattttta ctaggattaa atgtcaggaa ttgtgaaaaa gtgagtttaa
4561 atgtatttgg ctaaggtgta tgtaaacttc cgacttcaac tgtataggga tccgcttcct
4621 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
4681 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
4741 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
4801 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
4861 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
4921 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
4981 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
5041 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
5101 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
5161 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
5221 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
5281 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
5341 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
5401 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
5461 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
5521 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
5581 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
5641 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
5701 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
5761 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
5821 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
5881 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
5941 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
6001 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
6061 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
6121 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
6181 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
6241 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
6301 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
6361 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
6421 ttcaatatta ttgaagcat
//
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