pAAVLINK-ATR-B

LOCUS       pAAVLINK-ATR-B              7047 bp DNA     circular SYN 26-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..141
                     /label=AAV2 ITR
     protein_bind    156..189
                     /label=loxJTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          190..281
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             282..4203
                     /codon_start=1
                     /label=ATR
                     /translation="VVKIYDALIYMQVNSSFEDHILEDLCGMLSLPWIYSHSDDGCLKL
                     TTFAANLLTLSCRISDSYSPQAQSRCVFLLTLFPRRIFLEWRTAVYNWALQSSHEVIRA
                     SCVSGFFILLQQQNSCNRVPKILIDKVKDDSDIVKKEFASILGQLVCTLHGMFYLTSSL
                     TEPFSEHGHVDLFCRNLKATSQHECSSSQLKASVCKPFLFLLKKKIPSPVKLAFIDNLH
                     HLCKHLDFREDETDVKAVLGTLLNLMEDPDKDVRVAFSGNIKHILESLDSEDGFIKELF
                     VLRMKEAYTHAQISRNNELKDTLILTTGDIGRAAKGDLVPFALLHLLHCLLSKSASVSG
                     AAYTEIRALVAAKSVKLQSFFSQYKKPICQFLVESLHSSQMTALPNTPCQNADVRKQDV
                     AHQREMALNTLSEIANVFDFPDLNRFLTRTLQVLLPDLAAKASPAASALIRTLGKQLNV
                     NRREILINNFKYIFSHLVCSCSKDELERALHYLKNETEIELGSLLRQDFQGLHNELLLR
                     IGEHYQQVFNGLSILASFASSDDPYQGPRDIISPELMADYLQPKLLGILAFFNMQLLSS
                     SVGIEDKKMALNSLMSLMKLMGPKHVSSVRVKMMTTLRTGLRFKDDFPELCCRAWDCFV
                     RCLDHACLGSLLSHVIVALLPLIHIQPKETAAIFHYLIIENRDAVQDFLHEIYFLPDHP
                     ELKKIKAVLQEYRKETSESTDLQTTLQLSMKAIQHENVDVRIHALTSLKETLYKNQEKL
                     IKYATDSETVEPIISQLVTVLLKGCQDANSQARLLCGECLGELGAIDPGRLDFSTTETQ
                     GKDFTFVTGVEDSSFAYGLLMELTRAYLAYADNSRAQDSAAYAIQELLSIYDCREMETN
                     GPGHQLWRRFPEHVREILEPHLNTRYKSSQKSTDWSGVKKPIYLSKLGSNFAEWSASWA
                     GYLITKVRHDLASKIFTCCSIMMKHDFKVTIYLLPHILVYVLLGCNQEDQQEVYAEIMA
                     VLKHDDQHTINTQDIASDLCQLSTQTVFSMLDHLTQWARHKFQALKAEKCPHSKSNRNK
                     VDSMVSTVDYEDYQSVTRFLDLIPQDTLAVASFRSKAYTRAVMHFESFITEKKQNIQEH
                     LGFLQKLYAAMHEPDGVAGVSAIRKAEPSLKEQILEHESLGLLRDATACYDRAIQLEPD
                     QIIHYHGVVKSMLGLGQLSTVITQVNGVHANRSEWTDELNTYRVEAAWKLSQWDLVENY
                     LAADGKSTTWSVRLGQLLLSAKKRDITAFYDSLKLVRAEQIVPLSAASFERGSYQRGYE
                     YIVRLHMLCELEHSIKPLFQHSP"
     misc_feature    3223..3228
                     /label=SDS
     intron          4204..4244
                     /label=Splicing Donor
     misc_feature    4248..4281
                     /label=lox2272-JT1571
     repeat_region   4310..4450
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4310..4439
                     /label=AAV2 ITR
     rep_origin      4525..4980
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5262..5366
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5367..6227
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6398..6986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtataac ttcgtatagc atacattata
      181 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
      241 tattggtctt actgacatcc actttgcctt tctctccaca ggtggtgaaa atttatgatg
      301 ctttgattta tatgcaagta aacagttcat ttgaagatca tatcctggaa gatttatgtg
      361 gtatgctctc acttccatgg atttattccc attctgatga tggctgttta aagttgacca
      421 catttgccgc taatcttcta acattaagct gtaggatttc agatagctat tcaccacagg
      481 cacaatcacg atgtgtgttt cttctgactc tgtttccaag aagaatattc cttgagtgga
      541 gaacagcagt ttacaactgg gccctgcaga gctcccatga agtaatccgg gctagttgtg
      601 ttagtggatt ttttatctta ttgcagcagc agaattcttg taacagagtt cccaagattc
      661 ttatagataa agtcaaagat gattctgaca ttgtcaagaa agaatttgct tctatacttg
      721 gtcaacttgt ctgtactctt cacggcatgt tttatctgac aagttcttta acagaacctt
      781 tctctgaaca cggacatgtg gacctcttct gtaggaactt gaaagccact tctcaacatg
      841 aatgttcatc ttctcaacta aaagcttctg tctgcaagcc attccttttc ctactgaaaa
      901 aaaaaatacc tagtccagta aaacttgctt tcatagataa tctacatcat ctttgtaagc
      961 atcttgattt tagagaagat gaaacagatg taaaagcagt tcttggaact ttattaaatt
     1021 taatggaaga tccagacaaa gatgttagag tggcttttag tggaaatatc aagcacatat
     1081 tggaatcctt ggactctgaa gatggattta taaaggagct ttttgtctta agaatgaagg
     1141 aagcatatac acatgcccaa atatcaagaa ataatgagct gaaggatacc ttgattctta
     1201 caacagggga tattggaagg gccgcaaaag gagatttggt accatttgca ctcttacact
     1261 tattgcattg tttgttatcc aagtcagcat ctgtctctgg agcagcatac acagaaatta
     1321 gagctctggt tgcagctaaa agtgttaaac tgcaaagttt tttcagccag tataagaaac
     1381 ccatctgtca gtttttggta gaatcccttc actctagtca gatgacagca cttccgaata
     1441 ctccatgcca gaatgctgac gtgcgaaaac aagatgtggc tcaccagaga gaaatggctt
     1501 taaatacgtt gtctgaaatt gccaacgttt tcgactttcc tgatcttaat cgttttctta
     1561 ctaggacatt acaagttcta ctacctgatc ttgctgccaa agcaagccct gcagcttctg
     1621 ctctcattcg aactttagga aaacaattaa atgtcaatcg tagagagatt ttaataaaca
     1681 acttcaaata tattttttct catttggtct gttcttgttc caaagatgaa ttagaacgtg
     1741 cccttcatta tctgaagaat gaaacagaaa ttgaactggg gagcctgttg agacaagatt
     1801 tccaaggatt gcataatgaa ttattgctgc gtattggaga acactatcaa caggttttta
     1861 atggtttgtc aatacttgcc tcatttgcat ccagtgatga tccatatcag ggcccgagag
     1921 atatcatatc acctgaactg atggctgatt atttacaacc caaattgttg ggcattttgg
     1981 ctttttttaa catgcagtta ctgagctcta gtgttggcat tgaagataag aaaatggcct
     2041 tgaacagttt gatgtctttg atgaagttaa tgggacccaa acatgtcagt tctgtgaggg
     2101 tgaagatgat gaccacactg agaactggcc ttcgattcaa ggatgatttt cctgaattgt
     2161 gttgcagagc ttgggactgc tttgttcgct gcctggatca tgcttgtctg ggctcccttc
     2221 tcagtcatgt aatagtagct ttgttacctc ttatacacat ccagcctaaa gaaactgcag
     2281 ctatcttcca ctacctcata attgaaaaca gggatgctgt gcaagatttt cttcatgaaa
     2341 tatatttttt acctgatcat ccagaattaa aaaagataaa agccgttctc caggaataca
     2401 gaaaggagac ctctgagagc actgatcttc agacaactct tcagctctct atgaaggcca
     2461 ttcaacatga aaatgtcgat gttcgtattc atgctcttac aagcttgaag gaaaccttgt
     2521 ataaaaatca ggaaaaactg ataaagtatg caacagacag tgaaacagta gaacctatta
     2581 tctcacagtt ggtgacagtg cttttgaaag gttgccaaga tgcaaactct caagctcggt
     2641 tgctctgtgg ggaatgttta ggggaattgg gggcgataga tccaggtcga ttagatttct
     2701 caacaactga aactcaagga aaagatttta catttgtgac tggagtagaa gattcaagct
     2761 ttgcctatgg attattgatg gagctaacaa gagcttacct tgcgtatgct gataatagcc
     2821 gagctcaaga ttcagctgcc tatgccattc aggagttgct ttctatttat gactgtagag
     2881 agatggagac caacggccca ggtcaccaat tgtggaggag atttcctgag catgttcggg
     2941 aaatactaga acctcatcta aataccagat acaagagttc tcagaagtca accgattggt
     3001 ctggagtaaa gaagccaatt tacttaagta aattgggtag taactttgca gaatggtcag
     3061 catcttgggc aggttatctt attacaaagg ttcgacatga tcttgccagt aaaattttca
     3121 cctgctgtag cattatgatg aagcatgatt tcaaagtgac catctatctt cttccacata
     3181 ttctggtgta tgtcttactg ggttgtaatc aagaagatca gcaggaggtt tatgcagaaa
     3241 ttatggcagt tctaaagcat gacgatcagc ataccataaa tacccaagac attgcatctg
     3301 atctgtgtca actcagtaca cagactgtgt tctccatgct tgaccatctc acacagtggg
     3361 caaggcacaa atttcaggca ctgaaagctg agaaatgtcc acacagcaaa tcaaacagaa
     3421 ataaggtaga ctcaatggta tctactgtgg attatgaaga ctatcagagt gtaacccgtt
     3481 ttctagacct cataccccag gatactctgg cagtagcttc ctttcgctcc aaagcataca
     3541 cacgagctgt aatgcacttt gaatcattta ttacagaaaa gaagcaaaat attcaggaac
     3601 atcttggatt tttacagaaa ttgtatgctg ctatgcatga acctgatgga gtggccggag
     3661 tcagtgcaat tagaaaggca gaaccatctc taaaagaaca gatccttgaa catgaaagcc
     3721 ttggcttgct gagggatgcc actgcttgtt atgacagggc tattcagcta gaaccagacc
     3781 agatcattca ttatcatggt gtagtaaagt ccatgttagg tcttggtcag ctgtctactg
     3841 ttatcactca ggtgaatgga gtgcatgcta acaggtccga gtggacagat gaattaaaca
     3901 cgtacagagt ggaagcagct tggaaattgt cacagtggga tttggtggaa aactatttgg
     3961 cagcagatgg aaaatctaca acatggagtg tcagactggg acagctatta ttatcagcca
     4021 aaaaaagaga tatcacagct ttttatgact cactgaaact agtgagagca gaacaaattg
     4081 tacctctttc agctgcaagc tttgaaagag gctcctacca acgaggatat gaatatattg
     4141 tgagattgca catgttatgt gagttggagc atagcatcaa accacttttc cagcattctc
     4201 caggtaagta tcaaggttac aagacaggtt taaggagacc aataagctat tattcgtata
     4261 ggatacttta tacgaagtta tgaattccac gtgcggaccg agcggccgca ggaaccccta
     4321 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     4381 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4441 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4501 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4561 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
     4621 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4681 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4741 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4801 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
     4861 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
     4921 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     4981 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5041 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5101 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5161 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5221 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5281 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5341 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5401 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5461 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5521 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5581 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5641 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5701 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5761 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5821 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5881 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5941 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6001 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6061 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6121 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6181 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6241 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6301 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6361 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6541 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6781 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6841 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6901 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6961 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7021 ccttttgctg gccttttgct cacatgt
//