HPV-31neo

LOCUS       HPV-31neo               10477 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Neomycin selectable HPV31 genome.
ACCESSION   .
VERSION     .
KEYWORDS    HPV-31neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  TITLE     HPV-31 tyrosine 102 regulates interaction with E2 binding partners 
            and episomal maintenance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(2771..2791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             3284..4078
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8027..8218
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(8212..8234)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8320..8460
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8375..8394
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8475..8492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8646..9234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8726..8745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9405..10265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10028..10047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10266..10370)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10438..10456
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aagctttaat gcagttaaaa tgtcctcctt tattgattac atctaatata aatgcaggta
       61 aggatgacag atggccatac ctacatagca gactggtggt ttttacattt ccaaatccat
      121 ttccatttga caaaaacgga aatccagtat atgaattaag tgataaaaac tggaaatcct
      181 ttttctcaag gacgtggtgc agattaaatt tgcacgagga agaggacaaa gaaaacgatg
      241 gagactcttt ctcaacgttt aaatgtgtgt caggacaaaa tattagaaca ttatgaaaat
      301 gatagtaaac gactttgtga tcatatagac tattggaaac atattcgact tgaatgtgta
      361 ttaatgtata aagcaagaga aatgggaata cacagtatta accaccaggt ggtgccagcg
      421 ttgtcagtat caaaggccaa agccttacaa gctattgaac tacaaatgat gttggaaaca
      481 ttaaataaca ctgaatacaa aaatgaggac tggacaatgc agcaaacaag tcttgaactg
      541 tatttaactg cacctacagg gtgtttaaaa aaacatggat atactgtaga ggtgcaattt
      601 gatggtgatg tacacaacac catgcattat actaactgga aatttatata cctatgtata
      661 gatggccaat gtactgttgt ggaagggcaa gttaattgta agggcattta ttatgtacat
      721 gaaggacata taacatattt tgtaaatttt acagaagagg caaaaaaata tgggactggt
      781 aaaaaatggg aagtgcatgc gggtggtcag gtaattgttt ttcctgaatc tgtatttagc
      841 agtgacgaaa tatcctttgc tgggattgtt acaaagctac caacagccaa caacaccacc
      901 acatcgaatt ccaaaacctg cgccttgggc accagtgaag gtgtgcggcg ggcgacgacg
      961 tctactaagc gaccaagaac agagccagag cacagaaaca cccaccaccc caacaagttg
     1021 ttgcgaggcg actccgtgga cagtgtcaac tgtggggtta tcagtgcagc tgcatgcaca
     1081 aaccaaacaa gggctgtcag ttgtcctgca actacaccta taatacactt aaaaggtgat
     1141 gcaaatatat taaaatgttt aagatatagg ctgtcaaaat ataaacaatt gtatgaacaa
     1201 gtgtcatcta catggcattg gacatgtaca gatggaaaac ataaaaatgc tattgtaacc
     1261 ttaacatata taagtacatc acaaagagac gattttttaa atactgtaaa aatacctaac
     1321 acagtatcag tgtcaacagg atatatgact atttagccta atgattgaac taaatatttc
     1381 tacagtaagc attgtgctat gctttttgct ttgcttttgt gtgctactat ttgtgtgtct
     1441 tgtcatacgt ccacttgtgc tgtctgtgtc ggtatatgca acactactat tattaattgt
     1501 gattttatgg gttattgcaa cctctccatt acgttgtttt tgtatatatg ttgtgtttat
     1561 atatattcca ttatttgtaa ttcatacaca tgcatctttt ttaagtcaac agtaactttt
     1621 ttacttgtgt atactgttgt ttgtattggt attggtattg gtattggtat tggtattggt
     1681 ataataaact tttttacttt ttttttatta ttaccatgcg gtccaaacgc tctacaaaac
     1741 gcactaaacg tgcgtctgct acacaattat atcaaacatg taaagcagca ggtacttgtc
     1801 catcagacgt tatacctaaa atagaacata ctaccattgc agaccaaata ttaaggtatg
     1861 gtagtatggg tgtttttttt ggtgggttgg gtattgggtc cggctctggt actgggggtc
     1921 gcactggata tgtccctctt agtacacgtc cttctacagt atctgaggca agtataccta
     1981 ttagaccacc agttagcatt gaccctgtag gtcccttgga cccctctata gtaagtcttg
     2041 ttgaagaatc tggaattgtt gatgttggtg cccctgctcc tataccacac cctcctacaa
     2101 catctgggtt tgacattgct acaactgcag acacaacacc tgcaatttta gatgtaacaa
     2161 gtgttagcac acatgaaaat cctactttta ctgatccatc tgtattgcag cctcctacac
     2221 ctgcagaaac atcaggtcat ttactacttt catcatcatc tattagcaca cataattatg
     2281 aggaaatacc tatggataca tttattgttt ctactaataa tgaaaacata acaagtagca
     2341 cacccattcc aggggtgcgc cgtcctgcac gtttagggtt atatagtaag gctacacaac
     2401 aagtaaaagt tattgatcca acgtttctta gtgctccaaa acagctaatt acatatgaaa
     2461 accctgccta tgaaactgta aatgctgaag aatctttata cttttccaat acatcgcata
     2521 atatagcccc tgatcccgac tttctagata ttatagcatt acataggcct gcccttacct
     2581 cacgtaggaa cactgttaga tatagtagac taggtaataa acaaactttg cgcactcgta
     2641 gtggtgctac tattggtgca agggtgcatt attattatga tattagtagt attaatcctg
     2701 caggggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggctgaaag
     2761 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2821 agaagtatgc aaagcatgca tctcaattag tcagcaagca ggtgtggaaa gtccccaggc
     2881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtccca
     2941 ctagtggccc cccaccccag ccccaggctg actaattttt tttatttatg cagaggccca
     3001 ggccccctgg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3061 cttttgcaaa tcgcttctgc cttctccctc ctgtgagttt ggtaagtcac tgactgtcta
     3121 tgcctgggaa agggtgggca ggagatgggg cagtgcagga aaagtggcac tatgaaccct
     3181 gcagccctag tttaacaatt aatcataagc atagtataat acaactcact atagcaattg
     3241 tactaacctt cttctctttc ctctcctgac aggaggagcc atcatgattg aacaagatgg
     3301 cctacatgca ggttctccag ctgcctgggt tgagagactg tttggctatg actgggcaca
     3361 gcagaccatt ggttgctctg atgcagcagt tttcagactt tcagcccaag gcaggccagt
     3421 cctttttgta aagacagacc tcagtggggc tctcaatgag ctccaggatg aggctgccag
     3481 actctcctgg ttggcaacaa ctggggtccc ctgtgcagct gtccttgatg tggtcacaga
     3541 agctggaagg gactggctcc tactaggtga ggtgcctggg caggacctcc tttcctctca
     3601 cctagctcca gctgagaaag tgtcaatcat ggctgatgcc atgagaagac tccacaccct
     3661 tgacccagcc acctgcccct ttgaccacca ggccaagcac aggatagaga gggccagaac
     3721 caggatggag gctggcctgg tggaccaaga tgacttggat gaagaacacc agggcctggc
     3781 ccctgctgaa ctatttgcca ggctcaaggc atccatgcca gatggtgagg acctagtggt
     3841 gactcatggg gatgcctgcc ttcccaacat catggttgaa aatggaaggt tctctggctt
     3901 catagactgt ggcaggctgg gagtggctga caggtaccag gacattgccc tagcaaccag
     3961 ggacatagca gaagagctag ggggagagtg ggcagacagg ttcctagtgc tctatggcat
     4021 tgcagcccct gactcccaga gaattgcctt ctacagactt cttgatgagt tcttctaaag
     4081 ctagagcttt cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta
     4141 ctaaactggg ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat
     4201 ttattttcat tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg
     4261 tgggaggtca gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa
     4321 tacactatat cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg
     4381 caacagcccc tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt
     4441 gatttggagg ttaaagtttt gctatgctgt attttaaccg gtacaccagc aaaacgtaaa
     4501 aaaactaaaa agtaatggat gtgtatgtaa tacatgtgtc tgtatgtgta tgtgcttgtg
     4561 ctgtattgta tatgtgtgtg tttgtgtgtt atatatggta tatgtatgtt tatgtatgcg
     4621 tgtgtacttg tatatatgta tagtatgtta tgtgtgtatg tatgctatgt atgttaataa
     4681 atatgtgtat acctgtgtgt gttgtgtatg ttgtccttat atacacccta ttagtaacat
     4741 actattacta ttttataaac tattgttcct acttgttcct gctcctccca atagtcatgt
     4801 acttatttct gcctataatt taggtgtcac gccatagtaa aagttgtaca cccggtccgt
     4861 tttttgcaac taaagctact ccattttgat tttatgcagc cattttaaat ccctaaccgt
     4921 tttcggttgc attgtttaaa catgctagta caactatgct gatgcagtag ttctgcggtt
     4981 tttggtttcc tgaatactag tttttgccaa cattctggct tgtagtttcc tgcctaacac
     5041 accttgccaa catataatcc agtccaactt tgcaattata ctatgaatca tgtttgttta
     5101 aatacaactg tagttcaact atgtgtcatg cacatatatt atattatcct acacacctta
     5161 aactgctttt aggcacatat tttgtagatt atctatatcc ttgattgcag tgctggcttt
     5221 tgcacatgtt taaactgcca aggttgtgtc atgcattata aataagttgt atgttactca
     5281 tataattaat tgcatatagg tattacaccg ttttcggtta cagttttaca agcaattgtt
     5341 ctttttatac ttaataataa taatcttagt ataaaaaagt agggagtgac cgaaagtggt
     5401 gaaccgaaaa cggttggtat ataaagcaca tagtattttg tgcaaaccta cagacgccat
     5461 gttcaaaaat cctgcagaaa gacctcggaa attgcatgaa ctaagctcgg cattggaaat
     5521 accctacgat gaactaagat tgaattgtgt ctactgcaaa ggtcagttaa cagaaacaga
     5581 ggtattagat tttgcattta cagatttaac aatagtatat agggacgaca caccacacgg
     5641 agtgtgtaca aaatgtttaa gattttattc aaaagtaagt gaatttagat ggtatagata
     5701 tagtgtgtat ggaacaacat tagaaaaatt gacaaacaaa ggtatatgtg atttgttaat
     5761 taggtgtata acgtgtcaaa gaccgttgtg tccagaagaa aaacaaagac atttggataa
     5821 aaagaaacga ttccacaaca taggaggaag gtggacagga cgttgcatag catgttggag
     5881 aagacctcgt actgaaaccc aagtgtaaac atgcgtggag aaacacctac gttgcaagac
     5941 tatgtgttag atttgcaacc tgaggcaact gacctccact gttatgagca attacccgac
     6001 agctcagatg aggaggatgt catagacagt ccagctggac aagcagaacc ggacacatcc
     6061 aattacaata tcgttacctt ttgttgtcag tgtaagtcta cacttcgttt gtgtgtacag
     6121 agcacacaag tagatattcg catattgcaa gagctgttaa tgggctcatt tggaatcgtg
     6181 tgccccaact gttctactag actgtaacta caatggctga tccagcaggt acagatgggg
     6241 aggggacggg atgcaatggt tggttttatg tagaagcagt aattgacaga cagacagggg
     6301 acaacatttc agaggacgaa aatgaagaca gtagtgatac tggggaggat atggttgact
     6361 ttattgacaa ttgtaatgta tacaacaatc aggcagaagc agagacagca caggcattgt
     6421 ttcatgcaca ggaagcggag gaacatgcag aggctgtgca ggttctaaaa cgaaagtatg
     6481 taggtagtcc tttaagtgat attagtagtt gtgtggatta taatattagt ccacggttaa
     6541 aagctatatg catagaaaat aacagtaaaa cagcaaaacg aagactcttt gaacttccag
     6601 acagcgggta tggcaatact gaagtggaaa cgcagcagat ggtacaggta gaggagcaac
     6661 aaacaacatt aagttgtaat ggtagtgacg ggacacatag tgaacgagag aatgaaactc
     6721 caacacgtaa tatattgcaa gtgttaaaaa ctagcaatgg taaagctgct atgttaggta
     6781 aatttaaaga attatatggt gtaagtttta tggaactaat taggccattt caaagcaata
     6841 aaagcacatg tactgattgg tgtgtagctg cgtttggagt tacaggtaca gttgcagaag
     6901 gatttaaaac cctattgcaa ccatattgtt tgtattgcca tttacaaagt ttagcatgtt
     6961 cctggggcat ggttatgtta atgcttgtga gatttaaatg tgcaaaaaat agaataacaa
     7021 ttgaaaaatt attagaaaaa ttattgtgta tatctacaaa ttgtatgtta attcagccac
     7081 ccaaattacg tagcacagct gcagcattat attggtacag aacaggaatg tcaaacatta
     7141 gcgatgtata tggtgaaaca ccagaatgga tagaaagaca aacagtatta cagcatagtt
     7201 ttaatgacac aacatttgat ttgtcccaaa tggtacaatg ggcatatgac aatgatgtta
     7261 tggatgatag tgaaattgcc tataaatatg cacaattagc tgacagtgat agtaatgcat
     7321 gtgcattttt aaaaagtaat tcgcaggcaa aaatagttaa agattgtgga acaatgtgta
     7381 gacattataa acgagcagaa aaacgacaaa tgtccatggg acagtggatt aaaagtagat
     7441 gtgacaaagt tagtgacgaa ggtgactgga gggacatagt aaagttttta agatatcaac
     7501 aaatagaatt tgtgtcattt ttatctgcat taaagctgtt tttaaaagga gtgccaaaga
     7561 aaaactgtat tttaatacat ggtgcaccta atacaggtaa atcatatttt ggaatgagcc
     7621 ttattagctt tttacaagga tgtataatat catatgcaaa ttcaaaaagt catttttggt
     7681 tacaaccact ggctgatgct aaaataggca tgttagatga tgctacaacg ccatgttggc
     7741 attatataga caattaccta cgaaatgcac tagatggcaa ccctgtatct atagatgtaa
     7801 agcataaagc ttaaaattct catgtttgac agcttatcat cgctggcatt gaccctgagt
     7861 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
     7921 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
     7981 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
     8041 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     8101 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     8161 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     8221 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     8281 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     8341 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8401 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8461 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8521 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8581 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8641 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8701 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8761 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8821 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8881 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8941 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9001 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9061 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     9121 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9181 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9241 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9301 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9361 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9421 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9481 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9541 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9601 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9661 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9721 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9781 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9841 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9901 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9961 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10021 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10081 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10141 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10201 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10261 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10321 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10381 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    10441 aggcgtatca cgaggccctt tcgtcttcaa gaatttt
//