| 质粒名称: | AID-BE3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 100803 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3402 bp |
| 完整质粒大小: | 8439 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | activation induced cytidine deaminase |
| 物种来源: | H、 智人；化脓性链球菌 |
| 插入大小: | 5037 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 吉布森克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 |
| 3' 测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | AID-BE3 was a gift from David Liu (Addgene plasmid # 100803 ; http://n2t.net/addgene:100803 ; RRID:Addgene_100803) |
| 参考文献部分: | Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. aao4774 [pii] PubMed 28875174 |
买家导航
AID-BE3载体 AIDBE3质粒基本信息
AID-BE3质粒图谱 AIDBE3载体图谱和AID-BE3载体序列 AIDBE3质粒序列多克隆位点信息
AID-BE3质粒 AIDBE3载体简介
AID-BE3质粒序列 AIDBE3载体序列
LOCUS AID-BE3 8439 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses AID-BE3 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS AID-BE3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8439)
AUTHORS Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW,
Kim YB, Badran AH, Liu DR
TITLE Improved base excision repair inhibition and bacteriophage Mu Gam
protein yields C:G-to-T:A base editors with higher efficiency and
product purity.
JOURNAL Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774.
eCollection 2017 Aug.
PUBMED 28875174
REFERENCE 2 (bases 1 to 8439)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8439
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 403..412
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 409..1002
/codon_start=1
/gene="AICDA"
/product="human activation-induced cytidine deaminase
(Dickerson et al., 2003)"
/label=AID
/translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
CDS 1051..5151
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5164..5412
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5425..5445
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5454..5471
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5494..5511)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5500..5724
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5795..5811)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5795..5811)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5808..5830)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5819..5835
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5843..5873)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5888..5909
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6026..6043)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6197..6785)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6277..6296)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6956..7816)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7579..7598
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7817..7921)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8000..8019)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8191..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggatagcctc
421 ttgatgaata gacgcaagtt cctgtatcag tttaaaaacg tgagatgggc aaaaggccga
481 cgagagacat atctgtgcta tgtcgttaag cgcagagatt cagccaccag tttctctctc
541 gacttcggct acctgcggaa caagaatggt tgccatgttg agctcctgtt cctgaggtat
601 atcagcgact gggatttgga cccagggcgg tgctataggg tgacatggtt tacctcctgg
661 tcaccttgtt atgactgcgc gcggcatgtt gccgattttc tgagagggaa ccctaacctg
721 tctctgagga tcttcaccgc gcgactgtac ttctgtgagg accggaaagc cgaacccgag
781 ggactgagac gcctccacag agcgggtgtg cagattgcca taatgacctt taaggactac
841 ttctactgct ggaacacctt cgtcgaaaat cacgagcgga ctttcaaggc ttgggaagga
901 ttgcatgaaa acagcgtcag gctttccagg cagcttcgcc gcattcttct cccgttgtac
961 gaggttgatg acctcagaga tgcctttaga acactgggac tgagcggcag cgagactccc
1021 gggacctcag agtccgccac acccgaaagt gataaaaagt attctattgg tttagccatc
1081 ggcactaatt ccgttggatg ggctgtcata accgatgaat acaaagtacc ttcaaagaaa
1141 tttaaggtgt tggggaacac agaccgtcat tcgattaaaa agaatcttat cggtgccctc
1201 ctattcgata gtggcgaaac ggcagaggcg actcgcctga aacgaaccgc tcggagaagg
1261 tatacacgtc gcaagaaccg aatatgttac ttacaagaaa tttttagcaa tgagatggcc
1321 aaagttgacg attctttctt tcaccgtttg gaagagtcct tccttgtcga agaggacaag
1381 aaacatgaac ggcaccccat ctttggaaac atagtagatg aggtggcata tcatgaaaag
1441 tacccaacga tttatcacct cagaaaaaag ctagttgact caactgataa agcggacctg
1501 aggttaatct acttggctct tgcccatatg ataaagttcc gtgggcactt tctcattgag
1561 ggtgatctaa atccggacaa ctcggatgtc gacaaactgt tcatccagtt agtacaaacc
1621 tataatcagt tgtttgaaga gaaccctata aatgcaagtg gcgtggatgc gaaggctatt
1681 cttagcgccc gcctctctaa atcccgacgg ctagaaaacc tgatcgcaca attacccgga
1741 gagaagaaaa atgggttgtt cggtaacctt atagcgctct cactaggcct gacaccaaat
1801 tttaagtcga acttcgactt agctgaagat gccaaattgc agcttagtaa ggacacgtac
1861 gatgacgatc tcgacaatct actggcacaa attggagatc agtatgcgga cttatttttg
1921 gctgccaaaa accttagcga tgcaatcctc ctatctgaca tactgagagt taatactgag
1981 attaccaagg cgccgttatc cgcttcaatg atcaaaaggt acgatgaaca tcaccaagac
2041 ttgacacttc tcaaggccct agtccgtcag caactgcctg agaaatataa ggaaatattc
2101 tttgatcagt cgaaaaacgg gtacgcaggt tatattgacg gcggagcgag tcaagaggaa
2161 ttctacaagt ttatcaaacc catattagag aagatggatg ggacggaaga gttgcttgta
2221 aaactcaatc gcgaagatct actgcgaaag cagcggactt tcgacaacgg tagcattcca
2281 catcaaatcc acttaggcga attgcatgct atacttagaa ggcaggagga tttttatccg
2341 ttcctcaaag acaatcgtga aaagattgag aaaatcctaa cctttcgcat accttactat
2401 gtgggacccc tggcccgagg gaactctcgg ttcgcatgga tgacaagaaa gtccgaagaa
2461 acgattactc catggaattt tgaggaagtt gtcgataaag gtgcgtcagc tcaatcgttc
2521 atcgagagga tgaccaactt tgacaagaat ttaccgaacg aaaaagtatt gcctaagcac
2581 agtttacttt acgagtattt cacagtgtac aatgaactca cgaaagttaa gtatgtcact
2641 gagggcatgc gtaaacccgc ctttctaagc ggagaacaga agaaagcaat agtagatctg
2701 ttattcaaga ccaaccgcaa agtgacagtt aagcaattga aagaggacta ctttaagaaa
2761 attgaatgct tcgattctgt cgagatctcc ggggtagaag atcgatttaa tgcgtcactt
2821 ggtacgtatc atgacctcct aaagataatt aaagataagg acttcctgga taacgaagag
2881 aatgaagata tcttagaaga tatagtgttg actcttaccc tctttgaaga tcgggaaatg
2941 attgaggaaa gactaaaaac atacgctcac ctgttcgacg ataaggttat gaaacagtta
3001 aagaggcgtc gctatacggg ctggggacga ttgtcgcgga aacttatcaa cgggataaga
3061 gacaagcaaa gtggtaaaac tattctcgat tttctaaaga gcgacggctt cgccaatagg
3121 aactttatgc agctgatcca tgatgactct ttaaccttca aagaggatat acaaaaggca
3181 caggtttccg gacaagggga ctcattgcac gaacatattg cgaatcttgc tggttcgcca
3241 gccatcaaaa agggcatact ccagacagtc aaagtagtgg atgagctagt taaggtcatg
3301 ggacgtcaca aaccggaaaa cattgtaatc gagatggcac gcgaaaatca aacgactcag
3361 aaggggcaaa aaaacagtcg agagcggatg aagagaatag aagagggtat taaagaactg
3421 ggcagccaga tcttaaagga gcatcctgtg gaaaataccc aattgcagaa cgagaaactt
3481 tacctctatt acctacaaaa tggaagggac atgtatgttg atcaggaact ggacataaac
3541 cgtttatctg attacgacgt cgatcacatt gtaccccaat cctttttgaa ggacgattca
3601 atcgacaata aagtgcttac acgctcggat aagaaccgag ggaaaagtga caatgttcca
3661 agcgaggaag tcgtaaagaa aatgaagaac tattggcggc agctcctaaa tgcgaaactg
3721 ataacgcaaa gaaagttcga taacttaact aaagctgaga ggggtggctt gtctgaactt
3781 gacaaggccg gatttattaa acgtcagctc gtggaaaccc gccaaatcac aaagcatgtt
3841 gcacagatac tagattcccg aatgaatacg aaatacgacg agaacgataa gctgattcgg
3901 gaagtcaaag taatcacttt aaagtcaaaa ttggtgtcgg acttcagaaa ggattttcaa
3961 ttctataaag ttagggagat aaataactac caccatgcgc acgacgctta tcttaatgcc
4021 gtcgtaggga ccgcactcat taagaaatac ccgaagctag aaagtgagtt tgtgtatggt
4081 gattacaaag tttatgacgt ccgtaagatg atcgcgaaaa gcgaacagga gataggcaag
4141 gctacagcca aatacttctt ttattctaac attatgaatt tctttaagac ggaaatcact
4201 ctggcaaacg gagagatacg caaacgacct ttaattgaaa ccaatgggga gacaggtgaa
4261 atcgtatggg ataagggccg ggacttcgcg acggtgagaa aagttttgtc catgccccaa
4321 gtcaacatag taaagaaaac tgaggtgcag accggagggt tttcaaagga atcgattctt
4381 ccaaaaagga atagtgataa gctcatcgct cgtaaaaagg actgggaccc gaaaaagtac
4441 ggtggcttcg atagccctac agttgcctat tctgtcctag tagtggcaaa agttgagaag
4501 ggaaaatcca agaaactgaa gtcagtcaaa gaattattgg ggataacgat tatggagcgc
4561 tcgtcttttg aaaagaaccc catcgacttc cttgaggcga aaggttacaa ggaagtaaaa
4621 aaggatctca taattaaact accaaagtat agtctgtttg agttagaaaa tggccgaaaa
4681 cggatgttgg ctagcgccgg agagcttcaa aaggggaacg aactcgcact accgtctaaa
4741 tacgtgaatt tcctgtattt agcgtcccat tacgagaagt tgaaaggttc acctgaagat
4801 aacgaacaga agcaactttt tgttgagcag cacaaacatt atctcgacga aatcatagag
4861 caaatttcgg aattcagtaa gagagtcatc ctagctgatg ccaatctgga caaagtatta
4921 agcgcataca acaagcacag ggataaaccc atacgtgagc aggcggaaaa tattatccat
4981 ttgtttactc ttaccaacct cggcgctcca gccgcattca agtattttga cacaacgata
5041 gatcgcaaac gatacacttc taccaaggag gtgctagacg cgacactgat tcaccaatcc
5101 atcacgggat tatatgaaac tcggatagat ttgtcacagc ttgggggtga ctctggtggt
5161 tctactaatc tgtcagatat tattgaaaag gagaccggta agcaactggt tatccaggaa
5221 tccatcctca tgctcccaga ggaggtggaa gaagtcattg ggaacaagcc ggaaagcgat
5281 atactcgtgc acaccgccta cgacgagagc accgacgaga atgtcatgct tctgactagc
5341 gacgcccctg aatacaagcc ttgggctctg gtcatacagg atagcaacgg tgagaacaag
5401 attaagatgc tctctggtgg ttctcccaag aagaagagga aagtctaacc ggtcatcatc
5461 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
5521 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
5581 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
5641 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
5701 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
5761 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
5821 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
5881 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
5941 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
6001 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
6061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
6121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
6181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
6241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
6301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
6361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
6421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
6481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
6541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
6601 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
6661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
6721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
6781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
6841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
6901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
6961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
7021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
7081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
7141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
7201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
7261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
7321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
7381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
7441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
7501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
7561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
7621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
7681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
7741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
7801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
7861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
7921 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
7981 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
8041 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
8101 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
8161 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
8221 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
8281 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
8341 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
8401 tacggtaaac tgcccacttg gcagtacatc aagtgtatc
//
