pUBC-SYT1wt-mScarlet3

LOCUS       pUBC-SYT1wt-mScarlet3         6444 bp DNA     circular SYN 26-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pUBC-SYT1wt-mScarlet3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Ruizhao Wang
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      55..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             61..1326
                     /codon_start=1
                     /label=SYT1wt
                     /translation="MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKE
                     KFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINM
                     KDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGI
                     IQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGG
                     KTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSL
                     RYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYY
                     NESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPR
                     RPIAQWHTLQVEEEVDAMLAVKK"
     misc_feature    1162..1164
                     /label=Ile
     transit_peptide 1327..1401
                     /codon_start=1
                     /label=Linker25
                     /translation="GGSGGTGGSGGTGGSGGTGGSGGTG"
     CDS             1402..1428
                     /codon_start=1
                     /label=HA tag
                     /translation="YPYDVPDYA"
     CDS             1432..2118
                     /codon_start=1
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     regulatory      1432..1435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1830..1849
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2273..2328
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2689..2705)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2713..2729
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2737..2767)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     2742..2763
                     /label=lac-pro-F
     protein_bind    2782..2803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2862..3057
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2887..3057
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2908..3043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3063..3197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3436..4024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3440..3459)
                     /label=pUBC-primer-1
     CDS             complement(4195..5055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4226..4247)
                     /label=Amp-F
     primer_bind     4969..4989
                     /label=Amp-R-seq
     promoter        complement(5056..5159)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5232..5631
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(5359..5381)
                     /label=hUBC-pro-R
     primer_bind     5827..5850
                     /label=pUBC-seq-2
     promoter        5918..6444
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6281..6300
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gaagcttggg ctgcaggtcg agctcaagct tcgaattctg cagtcgacgg taccgccacc
       61 atggtgagcg agagtcacca tgaggccctg gcagccccgc ctgtcaccac tgtcgcgact
      121 gttctgccaa gcaatgccac agagccagcc agtcctggag aaggaaagga agatgcattt
      181 tctaagctga aggagaagtt tatgaatgag ttgcataaaa ttccattgcc accgtgggcc
      241 ttaattgcaa tagccatagt cgcagtcctt ttagtcctga cctgctgctt ttgtatctgt
      301 aagaaatgtt tgttcaaaaa gaaaaacaag aagaagggaa aggaaaaagg agggaagaat
      361 gccattaaca tgaaagatgt aaaagactta gggaagacga tgaaagatca ggccctcaag
      421 gatgatgatg ctgaaactgg attgacagat ggagaagaaa aagaagaacc caaagaagag
      481 gagaaactgg gaaaacttca gtattcactg gattatgatt tccaaaataa ccagctgctg
      541 gtagggatca ttcaggctgc cgaactgccc gccttggaca tggggggcac atctgatcct
      601 tacgtgaaag tgtttctgct acctgataag aagaagaaat ttgagacaaa agtccaccga
      661 aaaaccctta atcctgtctt caatgagcaa tttactttca aggtaccata ctcggaattg
      721 ggtggcaaaa ccctagtgat ggctgtatat gattttgatc gtttctctaa gcatgacatc
      781 attggagaat ttaaagtccc tatgaacaca gtggattttg gccatgtaac tgaggaatgg
      841 cgtgacctgc aaagtgctga gaaggaagag caagagaaat tgggtgatat ctgcttctcc
      901 cttcgctacg tacctactgc tggtaagctg actgttgtca ttctggaggc aaagaacctg
      961 aagaagatgg atgtgggtgg cttatccgat ccttatgtga agattcatct gatgcagaat
     1021 ggtaagaggc tgaagaagaa aaagacaaca attaaaaaga acacacttaa cccctactac
     1081 aatgagtcat tcagctttga agtacctttt gaacaaatcc agaaagtgca ggtggtggta
     1141 actgttttgg actatgacaa gattggcaag aacgatgcca tcggcaaagt ctttgtgggc
     1201 tacaacagca ccggcgcgga gctgcgacac tggtcagaca tgctggccaa ccccaggcga
     1261 cctattgccc agtggcacac cctgcaggta gaggaggaag ttgatgccat gctggccgtc
     1321 aagaagggcg gaagcggcgg gaccggcggc agcggaggga ccggggggag cggcggcacc
     1381 ggaggaagcg ggggaaccgg ttatccatac gacgtgcctg attatgcagg catggatagc
     1441 accgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
     1501 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1561 gccaagctga gggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
     1621 cagttcatgt acggctccag ggccttcacg aagcaccccg ccgacatccc cgactactgg
     1681 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     1741 gccgtgtccg tggcccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
     1801 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg
     1861 gaagcatcca ccgagcggtt gtaccccgag gacgtcgtgc tgaagggcga cattaagatg
     1921 gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac ctacagggcc
     1981 aagaagcccg tgcagatgcc cggcgccttc aacatcgacc gcaagttgga catcacatcc
     2041 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgtggcccg ccactccacc
     2101 ggcggctccg gtggctcctg aagcggccgc actcctcagg tgcaggctgc ctatcagaag
     2161 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     2221 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     2281 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     2341 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     2401 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     2461 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     2521 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     2581 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     2641 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     2701 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2761 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2821 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     2881 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     2941 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3001 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3061 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3121 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3181 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3241 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3841 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4021 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4081 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4141 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4201 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4261 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4321 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4381 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4441 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4501 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4561 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4621 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4681 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4741 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4801 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4861 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4921 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4981 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5041 atgttgaata ctcatactct tcctttttca tattattgaa gcatttatca gggttattgt
     5101 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5161 acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagta acccgtgtcg
     5221 gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     5281 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     5341 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     5401 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     5461 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     5521 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     5581 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     5641 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     5701 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     5761 tgggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     5821 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     5881 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     5941 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     6001 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     6061 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     6121 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     6181 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     6241 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     6301 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     6361 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     6421 ccgtttttgg cttttttgtt agac
//