| 质粒名称: | pmiRFP2-P2A-HO1-N1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000238 |
| 产品用途: | Expresses miRFP2 and HO1 in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | miRFP2, HO1 |
| 物种来源: | Synthetic, Homo sapiens |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rapid directed molecular evolution of fluorescent proteins in mammalian cells
Babakhanova S., Jung E.E., Namikawa K., Zhang H., Wang Y., Subach O.M., Korzhenevskiy D.A., Rakitina T.V., Xiao X., Wang W., Shi J., Drobizhev M., Park D., Eisenhard L., Tang H., Köster R.W., Subach F.V., Boyden E.S., Piatkevich K.D.
Protein Sci. 31, 728–751 (2022)
|
pmiRFP2-P2A-HO1-N1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pmiRFP2-P2A-HO1-N1载体质粒基本信息
pmiRFP2-P2A-HO1-N1质粒图谱载体图谱和pmiRFP2-P2A-HO1-N1载体序列质粒序列多克隆位点信息
pmiRFP2-P2A-HO1-N1质粒载体简介
pmiRFP2-P2A-HO1-N1质粒序列载体序列
LOCUS pmiRFP2-P2A-HO1-N1 5671 bp DNA circular SYN 27-APR-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5671)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Jul 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5671
/lab_host="Escherichia coli"
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-Fw
misc_feature 591..595
/label=MCS
/note="multiple cloning site"
misc_feature 597..602
/label=Kozak
CDS 603..1541
/codon_start=1
/label=miRFP2
/translation="MVAGHASGSPDFGTASPSDCEHEEIHLAGSIQPHGTLLVVSEPDH
RIIQASANAAEFLNLGSVLGIPLAEIDGDLLIKILPHLDLTAEGMPVAVRCRIGNPSTE
YDGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
YDRVMVYRFDERGHGEVYSEIHVTGLESYFGNRYPSSLVPQMARRLYVRQRVRVLVDVT
YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPTHLQFLKNMGVRATLVVSLVVGGKLWGLV
ICHHYLPRFIHFELRAICVLLAEAIATRITAL"
misc_feature 651..653
/label=Feature 3
unsure 742
/label=Val
unsure 982..983
/label=Ser
unsure 1065..1067
/label=Val
CDS 1203..1205
/label=Thr
unsure 1233
/label=Val
unsure 1267
/label=Thr
CDS 1359..1361
/label=Cys
unsure 1381
/label=Asp
unsure 1404..1406
/label=Ala
primer_bind complement(1542..1588)
/label=P2A-Rv
CDS 1557..1613
/codon_start=1
/label=P2A
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1614..2336
/codon_start=1
/label=HO1
/translation="MSVNLASQLREGTKKSHSMAENVGFVKCFLKGVVEKNSYRKLVGN
LYFVYSAMEEEMAKFKDHPILSHIYFPELNRKQSLEQDLQFYYGSNWRQEVKISAAGQA
YVDRVRQVAATAPELLVAHSYTRYLGDLSGGQILKKIAQNAMNLHDGGTAFYEFADIDD
EKAFKNTYRQAMNDLPIDQATAERIVDEANDAFAMNMKMFNELEGNLIKAIGIMVFNSL
TRRRSQGSTEVGLATSEG"
polyA_signal 2459..2580
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2587..3042)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3069..3173
/gene="bla"
/label=AmpR promoter
promoter 3175..3532
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3383..3518
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3567..4361
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4593..4640
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4969..5557
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
601 ccatggtcgc aggacacgca agtggaagcc ccgactttgg aaccgccagt ccgagcgact
661 gtgagcacga agaaatccat ctggccggat ccatccagcc acacggaaca ctgctggtgg
721 tctccgagcc tgaccataga ataattcagg catctgccaa cgccgctgaa ttcctgaatc
781 tgggaagtgt gctgggaatc cccctggctg agatcgacgg ggatctactg atcaagattc
841 tgccccacct ggaccttacc gcagaaggaa tgccagtggc cgtcaggtgc cgcattggaa
901 acccctccac agagtacgac ggactgatgc atcgaccacc tgaaggagga ctgatcattg
961 agctggaacg ggctgggcca tccattgatc tgagcggaac actggctccc gcactggagc
1021 gaattcgaac tgcaggatcc ctgagggctc tgtgcgacga tactgtactg ctgttccagc
1081 agtgtaccgg ctacgaccga gtgatggtct atcggttcga tgaacggggg cacggagagg
1141 tgtattcaga aattcatgtg actggactgg agagctactt tggcaacagg tatcctagct
1201 ccctcgtgcc acagatggcc cggcggctgt acgtgagaca gagggtgcgc gtcctggtag
1261 atgtcaccta tcagcctgtg ccactggagc caagactgag tcccctgact ggcagggacc
1321 tggatatgtc agggtgtttt ctgcgctcta tgagtcctac tcacctgcag ttcctgaaga
1381 acatgggcgt gcgggcaaca ctggtggtca gcctggtggt cggaggcaaa ctgtgggggc
1441 tggtgatctg ccaccattat ctgccacgct tcatccattt tgagctgcga gcaatttgtg
1501 tgctgctggc cgaagctatc gcaacccgca ttacagccct ggaattcgga tccggagcta
1561 ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga cctatgagtg
1621 tcaacttagc ttcccagttg cgggaaggga cgaaaaaatc ccactccatg gcggagaacg
1681 tcggctttgt caaatgcttc ctcaagggcg ttgtcgagaa aaattcctac cgtaagctgg
1741 ttggcaatct ctactttgtc tacagtgcca tggaagagga aatggcaaaa tttaaggacc
1801 atcccatcct cagccacatt tacttccccg aactcaaccg caaacaaagc ctagagcaag
1861 acctgcaatt ctattacggc tccaactggc ggcaagaagt gaaaatttct gccgctggcc
1921 aagcctatgt ggaccgagtc cggcaagtgg ccgctacggc ccctgaattg ttggtggccc
1981 attcctacac ccgttacctg ggggatcttt ccggcggtca aattctcaag aaaattgccc
2041 aaaatgccat gaatctccac gatggtggca cagctttcta tgaatttgcc gacattgatg
2101 acgaaaaggc ttttaaaaat acctaccgtc aagctatgaa tgatctgccc attgaccaag
2161 ccaccgccga acggattgtg gatgaagcca atgacgcctt tgccatgaac atgaaaatgt
2221 tcaacgaact tgaaggcaac ctgatcaagg cgatcggcat tatggtgttc aacagcctca
2281 cccgtcgccg cagtcaaggc agcaccgaag ttggcctcgc cacctccgaa ggctaaagcg
2341 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
2401 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
2461 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
2521 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
2581 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
2641 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
2701 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
2761 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
2821 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
2881 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
2941 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
3001 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
3061 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
3121 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
3181 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
3241 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
3301 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
3361 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
3421 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
3481 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
3541 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
3601 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
3661 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
3721 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
3781 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
3841 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
3901 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
3961 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
4021 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
4081 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
4141 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
4201 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
4261 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
4321 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
4381 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
4441 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
4501 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
4561 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
4621 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
4681 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
4741 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
4801 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
4861 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
4921 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
4981 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
5041 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
5101 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
5161 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
5221 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
5281 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
5341 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
5401 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
5461 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
5521 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
5581 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
5641 ttctgtggat aaccgtatta ccgccatgca t
//
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