| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-EP400-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001750 |
| 产品用途: | 3' AAVLINK plasmid for EP400 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EP400 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-EP400-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-EP400-C-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-EP400-C-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-EP400-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-EP400-C-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-EP400-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-EP400-C-FLAG 7278 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7278)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7278
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3991
/codon_start=1
/label=EP400
/translation="VPFAKPLPTFAKPTAEPGQDNPEWLISEDWALLQAVKQLLELPLN
LTIVSPAHTPNWDLVSDVVNSCSRIYRSSKQCRNRYENVIIPREEGKSKNNRPLRTSQI
YAQDENATHTQLYTSHFDLMKMTAGKRSPPIKPLLGMNPFQKNPKHASVLAESGINYDK
PLPPIQVASLRAERIAKEKKALADQQKAQQPAVAQPPPPQPQPPPPPQQPPPPLPQPQA
AGSQPPAGPPAVQPQPQPQPQTQPQPVQAPAKAQPAITTGGSAAVLAGTIKTSVTGTSM
PTGAVSGNVIVNTIAGVPAATFQSINKRLASPVAPGALTTPGGSAPAQVVHTQPPPRAV
GSPATATPDLVSMATTQGVRAVTSVTASAVVTTNLTPVQTPARSLVPQVSQATGVQLPG
KTITPAHFQLLRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTTTTSQVQVPQIQGQAQS
PAQIKAVGKLTPEHLIKMQKQKLQMPPQPPPPQAQSAPPQPTAQVQVQTSQPPQQQSPQ
LTTVTAPRPGALLTGTTVANLQVARLTRVPTSQLQAQGQMQTQAPQPAQVALAKPPVVS
VPAAVVSSPGVTTLPMNVAGISVAIGQPQKAAGQTVVAQPVHMQQLLKLKQQAVQQQKA
IQPQAAQGPAAVQQKITTQQITTPGAQQKVAYAAQPALKTQFLTTPISQAQKLAGAQQV
QTQIQVAKLPQVVQQQTPVASIQQVASASQQASPQTVALTQATAAGQQVQMIPAVTATA
QVVQQKLIQQQVVTTASAPLQTPGAPNPAQVPASSDSPSQQPKLQMRVPAVRLKTPTKP
PCQ"
misc_feature 1812..1817
/label=SDS
CDS 3998..4063
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4067..4498
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4211..4216
/label=SDS
repeat_region 4541..4681
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4541..4670
/label=AAV2 ITR
rep_origin 4756..5211
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5493..5597
/gene="bla"
/label=AmpR promoter
CDS 5598..6458
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6629..7217
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgc cattcgccaa
1561 gcccctgcca acttttgcca aacccacagc tgagcctggt caagacaacc ccgagtggct
1621 catcagtgag gactgggcgc tgctgcaggc tgtaaagcag ttactggagc tgcctttgaa
1681 cctcacaatc gtgtcacctg ctcacacacc taattgggat cttgtcagtg acgttgttaa
1741 ctcctgtagc cgaatctacc gctcttccaa acagtgccgg aatcgctacg agaatgtcat
1801 cattccacga gaggagggga agagtaaaaa caaccgtcct ctccgtacga gccagatcta
1861 tgcccaggat gagaatgcca cacacaccca gctgtacacg agccactttg acttaatgaa
1921 aatgactgct ggcaagagga gtcccccaat caaacctctg cttggcatga atccctttca
1981 gaagaacccc aagcacgcgt ctgtgttggc agaaagtgga atcaactatg acaagccgct
2041 gcctcccatc caggtggcat ctctccgtgc agagcgaatc gcaaaagaga aaaaggctct
2101 ggctgatcag cagaaggcac agcagccggc cgtggcccag ccacccccgc cccagccgca
2161 gcccccacca cccccgcagc agccaccgcc accgctgcca caaccacagg cagcgggcag
2221 ccagccgcca gcagggccac cagctgtcca gccccaaccc cagccacagc cccagaccca
2281 gccacagcct gtgcaggccc cagcgaaggc gcagcccgca atcacgacgg ggggcagtgc
2341 agccgtactg gcaggaacca ttaaaacatc agttactggg acgagcatgc ccactggtgc
2401 cgtgagtgga aatgtgatcg tgaacaccat cgcaggggtc ccagctgcca ccttccagtc
2461 catcaacaag cgcctggcgt cgccagtggc tcctggggcc ttgactacgc cgggaggctc
2521 tgctcccgcc caggtggtgc acacccagcc cccgccacgg gcagtcggct ccccagccac
2581 ggcgacccct gacctggtgt ccatggcaac gactcagggt gttcgagcgg tcacttctgt
2641 gacagcctcg gccgtggtca ctaccaacct gaccccagtg cagaccccgg cacggtcttt
2701 ggtgccccaa gtgtcccaag ccacaggagt tcagctccct ggaaaaacca tcacacctgc
2761 acatttccag cttctcaggc agcagcagca gcagcagcaa caacagcagc agcagcagca
2821 gcagcagcag cagcagcagc agcagcaaca gcagcagcag caacagacga cgacgacctc
2881 tcaggtgcaa gttccacaga tccagggcca ggcccagtcc ccagcacaga tcaaagctgt
2941 gggcaagctg acgccggaac acctcatcaa aatgcagaag cagaaactgc agatgccccc
3001 gcagccccca ccgccacagg cccagtctgc gcccccgcag ccaacagccc aagtgcaagt
3061 gcagacctcg cagccgccgc agcagcagag cccccagctc acgacggtca cggccccaag
3121 gcctggtgcc ctgctgacgg gcaccaccgt ggccaacctc caggtggccc ggctcacccg
3181 ggttcccact tctcagctgc aggcgcaagg gcagatgcag acccaggcac cccagccagc
3241 ccaggtggcc ttggcgaagc ctccggtggt gtccgtcccg gcagctgtgg tctcctcacc
3301 gggagtcacc accctgccca tgaacgtcgc ggggatcagc gtggcgatcg gtcagccaca
3361 gaaggcagca ggacagaccg tggtggccca gcccgtgcac atgcagcagc tgctgaagct
3421 gaagcagcag gccgtccagc agcagaaggc catccagccc caggctgcac agggcccggc
3481 agccgtccag cagaagatca ccacacagca gatcaccacc cctggcgcgc agcagaaggt
3541 tgcctacgcc gcgcagccgg cccttaagac ccagtttctt accacaccca tctcccaggc
3601 ccagaaactg gccggggccc agcaagtgca gacccagatc caggttgcaa aacttcctca
3661 agttgttcaa cagcaaacac ccgtggccag catccagcaa gttgcctctg cttcccagca
3721 ggcttctcca cagactgtgg cgctcacgca ggcgacggcg gccgggcagc aggtgcagat
3781 gatccctgca gtgaccgcga ctgcccaggt ggttcagcag aaactcattc agcagcaggt
3841 ggtgaccacg gcgtcggccc cgctccagac tccaggcgct cccaacccag cccaggtgcc
3901 cgccagctcc gacagcccaa gccagcagcc caagttacag atgagggtcc ctgctgtcag
3961 gctaaagaca cctactaagc ctccgtgcca gtgtacagac tacaaagacc atgacggtga
4021 ttataaagat catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa
4081 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
4141 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
4201 ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc
4261 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
4321 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga
4381 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
4441 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
4501 atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag
4561 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
4621 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
4681 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
4741 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
4801 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
4861 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
4921 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
4981 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
5041 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
5101 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
5161 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
5221 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
5281 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
5341 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
5401 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
5461 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
5521 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
5581 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
5641 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
5701 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
5761 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
5821 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
5881 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
5941 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
6001 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
6061 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
6121 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
6181 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
6241 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
6301 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
6361 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
6421 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
6481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
6541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
6601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
6661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
6721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
6781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
6841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
6901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
6961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
7021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
7081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
7141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
7201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
7261 ggccttttgc tcacatgt
//
