| 质粒名称: | pSCF82 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002798 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for Type-N TMP-inducible insulin secretion (PhCMV-SP-ecDHFRY100I-FCS-mINS-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pcDNA3.1
|
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
Stbl3
|
|
插入基因信息 |
|
| 插入基因名称: | ecDHFRY100I /mINS |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pSCF82
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSCF82载体质粒基本信息
pSCF82质粒图谱载体图谱和pSCF82载体序列质粒序列多克隆位点信息
pSCF82质粒载体简介
pSCF82质粒序列载体序列
LOCUS pSCF82 4713 bp DNA circular SYN 15-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4713)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4713
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
primer_bind 115..136
/label=oSCL1014
enhancer 154..533
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 534..737
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 688..708
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 748..806
/label=oSCL2204
primer_bind 749..812
/label=oSCL1108
primer_bind 750..776
/label=oSCL1017
primer_bind 755..806
/label=olyl539
primer_bind 763..802
/label=oSCF84
misc_feature 763..768
/label=NheI
primer_bind 765..806
/label=oSCL1968
primer_bind 765..798
/label=olyl396
primer_bind 779..800
/label=olyl612
misc_feature 779..783
/label=New Feature
misc_feature 779..783
/label=New Feature
primer_bind 789..812
/label=oSCL084
misc_feature 791..859
/label=Secretory (IgK)
/label=nonstandard type: signal
primer_bind complement(857..897)
/label=olyl540
primer_bind complement(857..882)
/label=olyl500
primer_bind 866..893
/label=olyl339
primer_bind 866..893
/label=olyl345
primer_bind 866..893
/label=olyl347
primer_bind complement(876..910)
/label=olyl547
CDS 878..1354
/codon_start=1
/label=ecDHFR(Y100I)
/translation="MISLIAALAVDHVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
primer_bind 878..901
/label=olyl541
misc_feature 881..883
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
primer_bind 894..933
/label=olyl548
primer_bind complement(916..937)
/label=OGT61-mDHFR-R
primer_bind 922..946
/label=OGT61-mDHFR-2-F
primer_bind complement(1145..1183)
/label=OLYL551
primer_bind complement(1147..1177)
/label=olyl549
primer_bind 1159..1196
/label=olyl550
primer_bind complement(1165..1185)
/label=OGT62-mDHFR-R
primer_bind 1170..1190
/label=OGT62-mDHFR-2-F
primer_bind 1177..1204
/label=OLYL552
primer_bind complement(1330..1360)
/label=olyl542
primer_bind complement(1343..1384)
/label=oSCF153
primer_bind complement(1348..1360)
/label=OLYL595
primer_bind 1361..1406
/label=oSCF154
CDS 1385..1642
/label=mINS
primer_bind complement(1625..1669)
/label=oSCF155
primer_bind complement(1625..1656)
/label=oSCL029
primer_bind complement(1625..1643)
/label=oSCL789
primer_bind complement(1625..1643)
/label=oSCL1111
primer_bind complement(1625..1643)
/label=oSCL1315
primer_bind complement(1625..1643)
/label=oSCL1909
primer_bind complement(1625..1643)
/label=oSCL1970
misc_feature 1669..1674
/label=EcoRI
misc_feature 1708..1713
/label=New Feature
misc_feature 1708..1713
/label=New Feature
terminator 1742..1969
/label=bGH PA
primer_bind complement(1951..1991)
/label=oSCL1014
primer_bind complement(1951..1973)
/label=oSCL1018
repeat_region 1976..2116
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 1976..2105
/label=AAV2 ITR
rep_origin 2191..2646
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2278..2297)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2488..2509
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2663..2682)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2782..2804
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(2842..2860)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 2928..3032
/gene="bla"
/label=AmpR promoter
CDS 3033..3893
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3251..3270)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4064..4652
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4553..4572
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat
181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
721 tctatataag cagagctctc tggctaacta ccggccaagc tggctagcgt ttaaacttaa
781 gcttgccacc atgactagtg agacagacac actcctgcta tgggtactgc tgctctgggt
841 tccaggttcc actggtgacg ctagtggtgg ttctggtatg atcagcctga tcgccgccct
901 ggccgtggac cacgtgatcg gcatggagaa cgcgatgccc tggaacctgc ccgccgacct
961 ggcctggttc aagagaaaca ccctgaacaa gcccgtgatc atgggcagac acacctggga
1021 gagcatcggc agacccctgc ccggcagaaa gaacatcatc ctgagcagcc agcccagcac
1081 cgacgacaga gtgacctggg tgaagagcgt ggacgaggcc atcgccgcct gcggcgacgt
1141 gcccgagatc atggtgatcg gcggcggcag agtgatcgag cagttcctgc ccaaggccca
1201 gaagctgtac ctgacccaca tcgacgccga ggtggagggc gacacccact tccccgacta
1261 cgagcccgac gactgggaga gcgtgttcag cgagttccac gacgccgacg cccagaacag
1321 ccacagctac tgcttcgaga tcctggagag aagagctagc tccgcacgaa accgtcagaa
1381 gaggtttgtc aaacagcacc tttgtggtcc tcacctggtg gaggctctgt acctggtgtg
1441 tggggaacgt ggtttcttct acacacccaa gtcccgtcgt aaaagggagg acccgcaagt
1501 gccacaactg gagctgggtg gaggcccgga ggccggggat cttcagacct tggcactgga
1561 ggttgcccgg cagaagcgtg gcattgtgga tcagtgctgc accagcatct gctccctcta
1621 ccaactggag aactactgca actaaggatc cactagtcca gtgtggtgga attctgcaga
1681 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc
1741 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
1801 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
1861 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
1921 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggg gccgcaggaa
1981 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
2041 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
2101 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
2161 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
2221 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
2281 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
2341 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
2401 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
2461 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
2521 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
2581 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
2641 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
2701 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
2761 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
2821 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
2881 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
2941 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
3001 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
3061 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
3121 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
3181 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
3241 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
3301 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
3361 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
3421 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
3481 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
3541 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
3601 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
3661 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
3721 ctgataaatc tggagccggt gagcgtggaa gccgcggtat cattgcagca ctggggccag
3781 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
3841 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
3901 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
3961 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
4021 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
4081 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
4141 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
4201 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
4261 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
4321 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
4381 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
4441 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
4501 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
4561 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
4621 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
4681 tcctggcctt ttgctggcct tttgctcaca tgt
//
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