pGT69

LOCUS      pGT69              6460 bp DNA     circular SYN 14-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6460
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     213..241
                     /label=OGT82-1-F
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     896..939
                     /label=OGT69-NLUC-F
     primer_bind     911..948
                     /label=OGT38-Nluc-F
     primer_bind     917..945
                     /label=OGT140-nluc-F
     primer_bind     920..943
                     /label=oSCL084
     primer_bind     921..946
                     /label=OGT71-NLUC-F
     misc_feature    922..990
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
     primer_bind     complement(988..1008)
                     /label=OGT150-1-R
     primer_bind     complement(990..1014)
                     /label=OGT84-1-R
     primer_bind     992..1008
                     /label=OGT150-2-F
     primer_bind     1009..1033
                     /label=OGT150-3-F
     primer_bind     complement(1009..1023)
                     /label=OGT150-2-R
     misc_feature    1012..1521
                     /label=NLuc (nano Luciferase)
                     /label=nonstandard type:  reporter
     primer_bind     1012..1036
                     /label=OGT84-3-F
     misc_feature    1042..1044
                     /label=cyt-STOP (TGG->TGA)
     primer_bind     complement(1505..1556)
                     /label=OGT69-NLUC-R
     primer_bind     complement(1505..1524)
                     /label=OGT140-nluc-R
     primer_bind     complement(1505..1521)
                     /label=OGT71-NLUC-R
     primer_bind     complement(1505..1521)
                     /label=OGT84-3-R
     primer_bind     complement(1511..1538)
                     /label=oSCL090
     primer_bind     complement(1511..1522)
                     /label=oSCL085
     primer_bind     1534..1560
                     /label=OGT150-2-F
     primer_bind     complement(1537..1557)
                     /label=OGT150-1-R
     misc_feature    1543..2021
                     /label=ecDHFR(N18T/A19V)
     primer_bind     complement(1543..1562)
                     /label=OGT84-1-R
     primer_bind     complement(1997..2019)
                     /label=OGT150-2-R
     terminator      2057..2284
                     /label=bGH PA
     misc_feature    2952..3029
                     /label=SV40 ORI
     CDS             3171..3962
                     /label=Kan/neoR
     primer_bind     4236..4260
                     /label=OGT140-nluc-R
     primer_bind     complement(4302..4322)
                     /label=M13-rev
     misc_binding    complement(4328..4350)
                     /label=LacO
     promoter        complement(4355..4384)
                     /label=lac
     CDS             complement(5467..6126)
                     /label=AmpR
     promoter        complement(6366..6394)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagctgccac catgactagt gagacagaca cactcctgct atgggtactg
      961 ctgctctggg ttccaggttc cactggtgac gctagtggtg gttctggtat ggtcttcaca
     1021 ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt
     1081 gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa
     1141 aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat
     1201 gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct
     1261 gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt
     1321 acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc
     1381 aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg
     1441 atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg
     1501 ctgtgcgaac gcattctggc gggaggcggc agcggagaat tcatgatcag cctgatcgcc
     1561 gccctggccg tggaccacgt gatcggcatg gagaccgtga tgccctggaa cctgcccgcc
     1621 gacctggcct ggttcaagag aaacaccctg aacaagcccg tgatcatggg cagacacacc
     1681 tgggagagca tcggcagacc cctgcccggc agaaagaaca tcatcctgag cagccagccc
     1741 agcaccgacg acagagtgac ctgggtgaag agcgtggacg aggccatcgc cgcctgcggc
     1801 gacgtgcccg agatcatggt gatcggcggc ggcagagtgt acgagcagtt cctgcccaag
     1861 gcccagaagc tgtacctgac ccacatcgac gccgaggtgg agggcgacac ccacttcccc
     1921 gactacgagc ccgacgactg ggagagcgtg ttcagcgagt tccacgacgc cgacgcccag
     1981 aacagccaca gctactgctt cgagatcctg gagagaagat agtctagagg gcccgtttaa
     2041 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2101 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2161 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2221 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     2281 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     2341 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2401 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2461 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2521 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2581 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2641 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     2701 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     2761 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2821 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2881 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     2941 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3001 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     3061 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     3121 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3181 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3241 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3301 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     3361 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3421 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3481 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3541 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3601 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3661 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     3721 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     3781 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     3841 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     3901 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     3961 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     4021 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4081 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     4141 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4201 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4261 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     4321 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4381 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4441 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4501 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4561 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4621 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4681 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4741 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4801 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4861 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4921 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4981 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5041 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5101 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5161 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5221 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5281 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5341 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5401 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5461 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5521 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5581 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5641 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5701 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5761 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5821 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5881 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5941 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6001 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6061 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6121 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6181 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6241 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6301 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6361 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6421 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//