| 质粒名称: | pBADHisB-Sumo-mBaoJin |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000274 |
| 产品用途: | Expresses Sumo-mBaoJin in bacteria |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 表达系统: | 细菌表达 |
| 启动子: | pBAD |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Sumo-mBaoJin |
| 物种来源: | Synthetic, synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Lesnov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D., Subach F.V.
Nat. Methods 2024 214 21, 657–665 (2024)
|
买家导航
pBADHisB-Sumo-mBaoJin载体质粒基本信息
pBADHisB-Sumo-mBaoJin质粒图谱载体图谱和pBADHisB-Sumo-mBaoJin载体序列质粒序列多克隆位点信息
pBADHisB-Sumo-mBaoJin质粒载体简介
pBADHisB-Sumo-mBaoJin质粒序列载体序列
LOCUS pBADHisB-Sumo-mBaoJin 5073 bp DNA circular SYN 17-NOV-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5073)
AUTHORS Vax
TITLE Direct Submission
JOURNAL Exported Apr 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT ORIGDB|GenBank
FEATURES Location/Qualifiers
source 1..5073
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 163..189
/label=ARABAD-F
CDS 331..348
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 352..384
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 388..411
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/label=Xpress(TM) tag
/translation="DLYDDDDK"
CDS 424..717
/codon_start=1
/label=Sumo(with deleted BglII site)
/label=Sumo(with\deleted\BglII\site)
/note="/vntifkey=21"
/translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
CDS 724..1428
/codon_start=1
/label=mBaoJin
/label=mStayGold
/note="/vntifkey=21"
/translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
EAHLKGMDELYK"
misc_feature 740..745
/label=SDS
terminator 1644..1730
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 1691..1713
/label=RRNBT1-R
promoter 1868..1959
/gene="bla"
/label=AmpR promoter
CDS 1960..2820
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
misc_feature 2821..3761
/label=ColE1
/note="ColE1"
rep_origin 2991..3579
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 3765..3905
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(4169..5047)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc
361 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccgagc
421 tcgatgtcgg actcagaagt caatcaagaa gctaagccag aggtcaagcc agaagtcaag
481 cctgagactc acatcaattt aaaggtgtcc gatggatctt cagagatttt cttcaagatc
541 aaaaagacca ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa
601 atggactcct taagattctt gtacgacggt attagaatcc aagctgatca gacccctgaa
661 gatttggaca tggaggataa cgatattatt gaggctcaca gagaacagat tggtggaaga
721 tctatggtct caaagggaga ggaggaaaac atggctagta caccatttaa atttcaactt
781 aaaggaacca tcaatggcaa atcgtttacc gttgaaggcg aaggtgaagg gaactcacat
841 gaaggttctc ataaaggaaa atatgtttgt acaagtggaa aactaccgat gtcatgggca
901 gcacttggga caacctttgg ttatggaatg aaatattata ccaaatatcc tagtggactg
961 aagaactggt ttcgtgaagt aatgcccgga ggctttacct acgatcgtca tattcaatat
1021 aaaggcgatg ggagtatcca tgcaaaacac caacacttta tgaaaaatgg gacttatcac
1081 aacattgtag aatttaccgg tcaggatttt aaagaaaata gtccagtctt aactggagat
1141 atgaatgtct cattaccgaa tgaagtccca caaataccca gagatgatgg agtagaatgc
1201 ccagtgacct tgctttatcc tttattatcg gataaatcaa aatacgtcga ggctcaccaa
1261 tatacaatct gcaagcctct tcataatcaa ccagcacctg atgtcccata tcactggatt
1321 cgtaaacaat acacacaaag caaagatgat gccgaggaac gcgatcatat ctgtcaatca
1381 gagactctcg aagcacactt aaagggcatg gacgagctgt ataagtagga attcgaagct
1441 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
1501 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
1561 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
1621 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
1681 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
1741 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
1801 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
1861 aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
1921 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
1981 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
2041 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
2101 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
2161 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
2221 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
2281 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
2341 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
2401 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
2461 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
2521 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
2581 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
2641 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
2701 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
2761 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
2821 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
2881 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
2941 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
3001 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
3061 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
3121 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
3181 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
3241 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
3301 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
3361 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
3421 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
3481 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
3541 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
3601 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
3661 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
3721 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
3781 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
3841 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
3901 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
3961 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
4021 cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg
4081 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
4141 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
4201 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
4261 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
4321 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
4381 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
4441 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
4501 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
4561 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
4621 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
4681 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
4741 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
4801 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
4861 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
4921 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
4981 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
5041 cagccatact tttcatactc ccgccattca gag
//
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