| 质粒名称: | pBADHisB-NMT |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000575 |
| 产品用途: | Expresses NMT in bacterial cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 表达系统: | 细菌表达 |
| 启动子: | araBAD |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | NMT |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Development of a genetically encoded NMT indicator for detection of mercury ions based on the green fluorescent protein mNeonGreen and metallothionein II from rat liver
Subach O.M., Piatkevich K.D., Subach F.V.
Heliyon 10, e32814 (2024)
|
买家导航
pBADHisB-NMT载体质粒基本信息
pBADHisB-NMT质粒图谱载体图谱和pBADHisB-NMT载体序列质粒序列多克隆位点信息
pBADHisB-NMT质粒载体简介
pBADHisB-NMT质粒序列载体序列
LOCUS pBADHisB-NMT 4896 bp DNA circular SYN 24-JUN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4896)
AUTHORS 1
TITLE Direct Submission
JOURNAL Exported Apr 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT ORIGDB|GenBank
FEATURES Location/Qualifiers
source 1..4896
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
misc_feature 305..310
/label=SDS
misc_feature 319..351
/label=His-tag
/note="/vntifkey=21"
CDS 331..348
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 352..384
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 388..411
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/label=Xpress(TM) tag
/translation="DLYDDDDK"
misc_feature 424
/label=Sumo(with deleted BglII site)
/label=Sumo(with\deleted\BglII\site)
/note="/vntifkey=21"
CDS 424
/codon_start=1
/gene="S. cerevisiae SMT3 (truncated)"
/product="cleavable ubiquitin-like protein tag"
/label=SUMO
/translation=""
misc_feature 430..1251
/label=NMT
/note="/vntifkey=21"
misc_feature 446..451
/label=SDS
terminator 1467..1553
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1645..1672
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1691..1782
/gene="bla"
/label=AmpR promoter
CDS 1783..2643
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
misc_feature 2644..3584
/label=ColE1
/note="ColE1"
rep_origin 2814..3402
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 3588..3728
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3992..4870)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc
361 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccgagc
421 tcgagatcta tggtctcaaa gggagaggag gaaaacatgg ctagtctgcc tgctacccac
481 gaactgcata tttttggctc cataaatggc atcgatttcg acatggtagg ccaaggcacc
541 ggtaatccaa acgacggcta tgaggagctg aacctgaagt ccaccatggg cgatctgcag
601 ttcagtccct ggatcctggt gccgcacatt gggtacgggt ttcaccagta tcttccctat
661 ccagacggga tgtcaccatt tcaggctgca atggtcgacg ggtccggata ccaagtgcac
721 cgcactatgc agtttgagga cggtgcctca ctgaccgtga attacagata cacctacgga
781 ggatcacata tcaaagggga agctcaggtg gagggcactg ggttcccagc tgacggccct
841 gtgatgacaa attccctgac agccgatact cttaaaagca gctgttcctg ctgccccgtg
901 ggctgtgtga agtgctccca gggctgcatc tgcaaagagg cttcggacaa gtgcagctgc
961 tgcgcccttg ttagtgctga ctggtgcgta tccaagaaga cttgccccaa tgataagact
1021 atcgtctcca catttaagtg ggcttttatc accgacaacg gcaaacgcta ccggtctacc
1081 gctcgcacca catacacatt cgccaagcca atggccgcaa attacctcaa aaatcagccg
1141 atgtatgtct ttcggaaaac agagctgaaa cattttaaga ccgaactgaa ttttaaagag
1201 tggcaaaagg cctttacgga cgtgatgggc atggacgagc tgtataagta ggaattcgaa
1261 gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg
1321 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga
1381 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca
1441 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
1501 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
1561 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat
1621 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
1681 tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
1741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
1801 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
1861 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
1921 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
1981 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
2041 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
2101 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
2161 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
2221 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
2281 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
2341 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
2401 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
2461 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
2521 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
2581 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
2641 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
2701 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
2761 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
2821 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
2881 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
2941 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
3001 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
3061 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
3121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
3181 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
3241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
3301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
3361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
3421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
3481 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
3541 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
3601 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
3661 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
3721 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
3781 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
3841 caccgtcatc accgaaacgc gcgaggcagc agatcaattc gcgcgcgaag gcgaagcggc
3901 atgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg
3961 tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca
4021 ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata
4081 cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca
4141 tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta
4201 agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa
4261 catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact
4321 gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca
4381 tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt
4441 ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt
4501 catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc
4561 agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac
4621 gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa
4681 attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac
4741 ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg
4801 ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg
4861 cttcagccat acttttcata ctcccgccat tcagag
//
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