| 质粒名称: | pAAVLINK-EFS-HA-5'-NSD1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001926 |
| 产品用途: | 5' AAVLINK plasmid for NSD1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NSD1 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EFS-HA-5'-NSD1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EFS-HA-5'-NSD1载体质粒基本信息
pAAVLINK-EFS-HA-5'-NSD1质粒图谱载体图谱和pAAVLINK-EFS-HA-5'-NSD1载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-5'-NSD1质粒载体简介
pAAVLINK-EFS-HA-5'-NSD1质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-5'-NSD1 7247 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7247)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7247
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 145..356
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 371..397
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 404..4387
/codon_start=1
/label=NSD1
/translation="DQTCELPRRNCLLPFSNPVNLDAPEDKDSPFGNGQSNFSEPLNGC
TMQLSTVSGTSQNAYGQDSPSCYIPLRRLQDLASMINVEYLNGSADGSESFQDPEKSDS
RAQTPIVCTSLSPGGPTALAMKQEPSCNNSPELQVKVTKTIKNGFLHFENFTCVDDADV
DSEMDPEQPVTEDESIEEIFEETQTNATCNYETKSENGVKVAMGSEQDSTPESRHGAVK
SPFLPLAPQTETQKNKQRNEVDGSNEKAALLPAPFSLGDTNITIEEQLNSINLSFQDDP
DSSTSTLGNMLELPGTSSSSTSQELPFCQPKKKSTPLKYEVGDLIWAKFKRRPWWPCRI
CSDPLINTHSKMKVSNRRPYRQYYVEAFGDPSERAWVAGKAIVMFEGRHQFEELPVLRR
RGKQKEKGYRHKVPQKILSKWEASVGLAEQYDVPKGSKNRKCIPGSIKLDSEEDMPFED
CTNDPESEHDLLLNGCLKSLAFDSEHSADEKEKPCAKSRARKSSDNPKRTSVKKGHIQF
EAHKDERRGKIPENLGLNFISGDISDTQASNELSRIANSLTGSNTAPGSFLFSSCGKNT
AKKEFETSNGDSLLGLPEGALISKCSREKNKPQRSLVCGSKVKLCYIGAGDEEKRSDSI
SICTTSDDGSSDLDPIEHSSESDNSVLEIPDAFDRTENMLSMQKNEKIKYSRFAATNTR
VKAKQKPLISNSHTDHLMGCTKSAEPGTETSQVNLSDLKASTLVHKPQSDFTNDALSPK
FNLSSSISSENSLIKGGAANQALLHSKSKQPKFRSIKCKHKENPVMAEPPVINEECSLK
CCSSDTKGSPLASISKSGKVDGLKLLNNMHEKTRDSSDIETAVVKHVLSELKELSYRSL
GEDVSDSGTSKPSKPLLFSSASSQNHIPIEPDYKFSTLLMMLKDMHDSKTKEQRLMTAQ
NLVSYRSPGRGDCSTNSPVGVSKVLVSGGSTHNSEKKGDGTQNSANPSPSGGDSALSGE
LSASLPGLLSDKRDLPASGKSRSDCVTRRNCGRSKPSSKLRDAFSAQMVKNTVNRKALK
TERKRKLNQLPSVTLDAVLQGDRERGGSLRGGAEDPSKEDPLQIMGHLTSEDGDHFSDV
HFDSKVKQSDPGKISEKGLSFENGKGPELDSVMNSENDELNGVNQVVPKKRWQRLNQRR
TKPRKRMNRFKEKENSECAFRVLLPSDPVQEGRDEFPEHRTPSASILEEPLTEQNHADC
LDSAGPRLNVCDKSSASIGDMEKEPGIPSLTPQAELPEPAVRSEKKRLRKPSKWLLEYT
EEYDQIFAPKKKQKKVQEQVHKVSSRCEEESLLARGRSSAQNKQ"
regulatory 1403..1412
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 3274..3279
/label=SDS
misc_feature 3990..3995
/label=SDS
intron 4388..4428
/label=Splicing Donor
misc_feature 4432..4465
/label=lox2272-JT1571
repeat_region 4510..4650
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4510..4639
/label=AAV2 ITR
repeat_region 4511..4651
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4725..5180
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5462..5566
/gene="bla"
/label=AmpR promoter
CDS 5567..6427
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6598..7186
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cgccaccatg tacccttatg acgtacctga ctatgctgga tctgatcaga cctgtgaact
421 acccagaaga aattgtctgc tgcccttttc caatccagtg aatttagatg cccctgaaga
481 caaggacagc cctttcggta atggtcaatc caatttttct gagccactta atgggtgtac
541 tatgcagtta tcgactgtca gtggaacatc ccaaaatgct tatggacaag attctccatc
601 ttgttacatt ccactgcgga gactacagga tttggcctcc atgatcaatg tagagtattt
661 aaatgggtct gctgatggat cagaatcctt tcaagaccct gaaaaaagtg attcaagagc
721 tcagacgcca attgtttgca cttccttgag tcctggtggt cctacagcac ttgctatgaa
781 acaggaaccc tcttgtaata actcccctga actccaggta aaagtaacaa agactatcaa
841 gaatggcttt ctgcactttg agaattttac ttgtgtggac gatgcagatg tagattctga
901 aatggaccca gaacagccag tcacagagga tgagagtata gaggagatct ttgaggaaac
961 tcagaccaat gccacctgca attatgagac taaatcagag aatggtgtaa aagtggccat
1021 gggaagtgaa caagacagca caccagagag tagacacggt gcagtcaaat cgccattctt
1081 gccattagct cctcagactg aaacacagaa aaataagcaa agaaatgaag tggacggcag
1141 caatgaaaaa gcagcccttc tcccagcccc cttttcacta ggagacacaa acattacaat
1201 agaagagcaa ttaaactcaa taaatttatc ttttcaggat gatccagatt ccagtaccag
1261 tacattagga aacatgctag aattacctgg aacttcatca tcatctactt cacaggaatt
1321 gccattttgt caacctaaga aaaagtctac gccactgaag tatgaagttg gagatctcat
1381 ctgggcaaaa ttcaagagac gcccatggtg gccctgcagg atttgttctg atccgttgat
1441 taacacacat tcaaaaatga aagtttccaa ccggaggccc tatcggcagt actacgtgga
1501 ggcttttgga gatccttctg agagagcctg ggtggctgga aaagcaatcg tcatgtttga
1561 aggcagacat caattcgaag agctacctgt ccttaggaga agagggaaac agaaagaaaa
1621 aggatatagg cataaggttc ctcagaaaat tttgagtaaa tgggaagcca gtgttggact
1681 tgcagaacag tatgatgttc ccaaggggtc aaagaaccga aaatgtattc ctggttcaat
1741 caagttggac agtgaagaag atatgccatt tgaagactgc acaaatgatc ctgagtcaga
1801 acatgacctg ttgcttaatg gctgtttgaa atcactggct tttgattctg aacattctgc
1861 agatgagaag gaaaagcctt gcgctaaatc tcgagccaga aagagctctg ataatccaaa
1921 aaggactagt gtgaaaaagg gccacataca atttgaagca cataaagatg aacggagggg
1981 aaagattcca gagaaccttg gcctaaactt tatctctggg gatatatctg atacgcaggc
2041 ctctaatgaa ctttccagga tagcaaatag cctcacaggg tccaacactg ccccaggaag
2101 ttttctgttt tcttcctgtg gaaaaaacac tgcaaagaaa gaatttgaga cttcaaatgg
2161 tgactcttta ttgggcttgc ctgagggtgc tttgatctca aagtgttctc gagagaagaa
2221 taaaccccaa cgaagcctgg tgtgtggttc aaaagtgaag ctctgctata ttggagcagg
2281 tgatgaggaa aagcgaagtg attccattag tatctgtacc acttctgatg atggaagcag
2341 tgacctggat cccatagaac acagctcaga gtctgataac agtgtccttg aaattccaga
2401 tgctttcgat agaacagaga acatgttatc tatgcagaaa aatgaaaaga taaagtattc
2461 taggtttgct gccacaaaca ctagggtaaa agcaaaacag aagcctctca ttagtaactc
2521 acatacagac cacttaatgg gttgtactaa gagtgcagag cctggaaccg agacgtctca
2581 ggttaatctc tctgatctga aggcatctac tcttgttcac aaaccccagt cagattttac
2641 aaatgatgct ctctctccaa aattcaacct gtcatcaagc atatccagtg agaactcgtt
2701 aataaagggt ggggcagcaa atcaagctct attacattcg aaaagcaaac agcccaagtt
2761 ccgaagtata aagtgcaaac acaaagaaaa tccagttatg gcagaacccc cagttataaa
2821 tgaggagtgc agtttgaaat gctgctcttc tgataccaaa ggctctcctt tggccagcat
2881 ttctaaaagt gggaaagtgg atggtctaaa actactgaac aatatgcatg agaaaaccag
2941 ggattcaagt gacatagaaa cagcagtggt gaaacatgtt ttatccgagt tgaaggaact
3001 ctcttacaga tccttaggtg aggatgtcag tgactctgga acatcaaagc catcaaaacc
3061 attacttttc tcttctgctt ctagtcagaa tcacatacct attgaaccag actacaaatt
3121 cagtacattg ctaatgatgt tgaaagatat gcatgatagt aagacgaagg agcagcggtt
3181 gatgactgct caaaacctgg tctcttaccg gagtcctggt cgtggggact gttctactaa
3241 tagtcctgta ggagtctcta aggttttggt ttcaggaggc tccacacaca attcagagaa
3301 aaagggagat ggcactcaga actccgccaa tcctagccct agtgggggtg actctgcatt
3361 atctggcgag ttgtctgctt ccctacctgg cttactgtcc gacaagagag acctccctgc
3421 ttctggtaaa agtcgttcag actgtgttac taggcgcaac tgtggacgat caaagccttc
3481 atccaaattg cgagatgctt tttcagccca aatggtaaag aacacagtga accgtaaagc
3541 cttaaagacc gagcgcaaaa gaaaactgaa tcagcttcca agtgtgactc ttgatgctgt
3601 actgcaggga gaccgagaac gtggaggttc attgagaggt ggggcagaag atcctagtaa
3661 agaggatccc cttcagataa tgggccactt aacaagtgaa gatggtgacc atttttctga
3721 tgtgcatttc gatagcaagg ttaagcaatc tgatcctggt aaaatttctg aaaaaggact
3781 ctcttttgaa aacggaaaag gcccagagct ggactctgta atgaacagtg agaatgatga
3841 actcaatggt gtaaatcaag tggtgcctaa aaagcggtgg cagcgtttaa accaaaggcg
3901 cactaaacct cgtaagcgca tgaacagatt taaagagaaa gaaaactctg agtgtgcctt
3961 tagggtctta cttcctagtg accctgtgca ggaggggcgg gatgagtttc cagagcatag
4021 aactccttca gcaagcatac ttgaggaacc actgacagag caaaatcatg ctgactgctt
4081 agattcagct gggccacggt taaatgtttg tgataaatcc agtgccagca ttggtgacat
4141 ggaaaaggag ccaggaattc ccagtttgac accacaggct gagctccctg aaccagctgt
4201 gcggtcagag aagaaacgcc ttaggaagcc aagcaagtgg cttttggaat atacagaaga
4261 atatgatcag atatttgctc ctaagaaaaa acaaaagaag gtacaggagc aggtgcacaa
4321 ggtaagttcc cgctgtgaag aggaaagcct tctagcccga ggtcgatcta gtgctcagaa
4381 caagcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg
4441 tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg
4501 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
4561 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
4621 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
4681 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
4741 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
4801 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
4861 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4921 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4981 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
5041 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
5101 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
5161 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
5221 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
5281 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
5341 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
5401 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
5461 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
5521 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
5581 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
5641 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
5701 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
5761 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
5821 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
5881 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5941 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
6001 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
6061 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
6121 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
6181 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
6241 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
6301 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
6361 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
6421 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
6481 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
6541 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
6601 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
6661 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
6721 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
6781 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
6841 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6901 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6961 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
7021 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
7081 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
7141 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
7201 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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