pCDH-PCaMKII-Mt-mScarlet-A-CREB

LOCUS       pCDH-PCaMKII-Mt-mScarlet-A-CREB               7520 bp DNA     circular SYN 02-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    2490-pCDH-PCaMKII-Mt-mScarlet-A-CREB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..44
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        59..285
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             286..466
                     /label=5' LTR
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    513..638
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1131..1364
                     /label=HIV-1 RRE
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
     misc_feature    1860..1976
                     /label=HIV-1 cPPT
                     /note="central polypurine tract from pol gene of human 
                     immunodeficiency virus type 1"
     promoter        1989..2359
                     /label=alpha-CamKII Promotor Truncated
     intron          2366..2467
                     /label=Mouse Insulin Intron 1-2
     primer_bind     2466..2509
                     /label=MA_F1 EcoRI
     regulatory      2492..2501
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2498..2677
                     /codon_start=1
                     /label=Mito-advanced Sequence
                     /translation="MAQRLLLRRFLASVISRKPSQGQWPPLTSRALQTPQCSPGGLTVT
                     PNPARTIYTTRISLT"
     CDS             2693..3388
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2826..2844)
                     /label=mCherry-R
     primer_bind     3094..3113
                     /label=mCherry-F
     primer_bind     complement(3368..3412)
                     /label=RedA XhoI R2
     CDS             3410..3595
                     /codon_start=1
                     /label=A-CREB
                     /translation="DPDLEQRAEELARENEELEKEAEELEQELAELENRVAVLENQNKT
                     LIEELKALKDLYCHKSD"
     iDNA            3561..3581
                     /label=shCREB?
     misc_feature    3634..4222
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     primer_bind     complement(3687..3707)
                     /label=WPRE-R
     CDS             complement(4105..4116)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4296..4529
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4601..4722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4762..4897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4930..4946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4954..4970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4978..5008)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5023..5044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5332..5920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6091..6951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6952..7056)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctgacgcgtg
       61 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
      121 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
      181 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
      241 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
      301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      361 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      481 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      541 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      601 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      661 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      721 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
      781 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
      841 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
      901 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
      961 ccaccgcaca gcaagcggcc actgatcttc agacctggag gaggagatat gagggacaat
     1021 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1081 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     1141 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1201 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1261 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1321 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1381 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1441 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1501 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1561 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1621 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1681 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1741 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1801 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     1861 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     1921 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     1981 atactagtac ttgtggacta agtttgttca catccccttc tccaaccccc tcagtacatc
     2041 accctgggag aacaaggtcc acttgcttct gggcccacac agtcctgcag tattgtgtat
     2101 ataaggccag ggcaacggag gagcaggttt tgaagtgaaa ggcaggcagg tgttggggag
     2161 gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat
     2221 gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct
     2281 cgtcaatcaa gctggttctc catttgcact caggagcacg ggcaggcgag tggcccctag
     2341 ttctgggagc agagtatcag atatcgtact cttctcagtg ggcctggctc cccagctaag
     2401 acctcaggga cttgaggtag gatatagcct cctctcttac gtgaaacttt tgctatcctc
     2461 aacccaggat atctctagtt gatcagaatt cgccaccatg gctcagcgac ttcttctgag
     2521 gaggttcctg gcctctgtca tctccaggaa gccctctcag ggtcagtggc cacccctcac
     2581 ttccagagcc ctccagaccc cacaatgcag tcctggtggc ctgactgtaa cacccaaccc
     2641 agcccggaca atatacacca cgaggatctc cttgacacct aggccaccgg ttatggtgag
     2701 caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat
     2761 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2821 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc
     2881 ccctcagttc atgtacggct ccagggcctt caccaagcac cccgccgaca tccccgacta
     2941 ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3001 cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt
     3061 gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg
     3121 ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa
     3181 gatggccctg cgcctgaagg acggaggccg ctacctggcg gacttcaaga ccacctacaa
     3241 ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtt gaccgcaagt tggacatcac
     3301 ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc
     3361 caccggcggc atggacgagc tgtacaagct cgagtctaga gcggccgcag atcctgacct
     3421 ggaacaacgt gctgaggaac tggcccgtga aaacgaagag ctggaaaaag aggccgaaga
     3481 gctggagcag gaactggcag aacttgagaa cagagtggca gtgcttgaaa accaaaacaa
     3541 aacattgatt gaggagctaa aagcacttaa ggacctttac tgccacaagt cagattaagt
     3601 ttaaactgta catagagctg aatctaagtc gacaatcaac ctctggatta caaaatttgt
     3661 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3721 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3781 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3841 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3901 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3961 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4021 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4081 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4141 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4201 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     4261 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     4321 aagataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     4381 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4441 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4501 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     4561 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     4621 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4681 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     4741 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4801 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4861 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt
     4921 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4981 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5041 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5101 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5161 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5221 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5281 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5341 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5401 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5461 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5521 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5581 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5641 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5701 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5761 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5821 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5881 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5941 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6001 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6061 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6121 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6181 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6241 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6301 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6361 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6421 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6481 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6541 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6601 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6661 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6721 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6781 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6841 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6901 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6961 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7021 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7081 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7141 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     7201 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     7261 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     7321 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7381 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     7441 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     7501 aacgccaggg ttttccagtc
//