| 质粒名称: | pCytERM-tdTomato |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000748 |
| 产品用途: | Expresses CytERM-tsTomato in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: |
tdTomato
|
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pCytERM-tdTomato
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCytERM-tdTomato载体质粒基本信息
pCytERM-tdTomato质粒图谱载体图谱和pCytERM-tdTomato载体序列质粒序列多克隆位点信息
pCytERM-tdTomato质粒载体简介
pCytERM-tdTomato质粒序列载体序列
LOCUS pCytERM-tdTomato 5523 bp DNA circular SYN 22-OCT-2024
DEFINITION TSN20241013-0571-00010_748_20241014-BAN4_G02_G02_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5523)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5523
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 424..727
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 728..931
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 983..1069
/codon_start=1
/label=cytochrome p450
/translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
regulatory 1115..1124
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1121..2551
/codon_start=1
/label=tdTomato
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
polyA_signal 2674..2795
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2802..3257)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3284..3388
/gene="bla"
/label=AmpR promoter
promoter 3390..3747
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3598..3733
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3782..4576
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4808..4855
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin join(5184..5523,1..249)
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
61 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
301 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
361 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
421 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
481 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
541 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
601 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
661 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
721 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
781 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
841 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
901 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
961 ctaccggact cagatctcca ccatggatcc tgtggtggtg ctggggctct gtctctcctg
1021 tttgcttctc ctttcactct ggaaacagag ctatggggga gggaagcttc gaattctgca
1081 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagta agggcgagga
1141 agtgatcaaa gagttcatgc ggtttaaggt gagaatggaa ggaagcatga acggccacga
1201 gttcgaaatt gagggagaag gagagggacg gccctacgag ggcacccaga cagccaagct
1261 gaaagtgaca aagggcgggc ctctgccatt cgcttgggac atcctgagcc cacagtttat
1321 gtacggctcc aaggcctatg tgaaacatcc agctgacatt cccgattata agaaactgag
1381 cttccccgag gggtttaagt gggaaagagt gatgaacttc gaggacggag gcctggtgac
1441 tgtgacccag gacagctccc tgcaggatgg gaccctgatc tacaaggtga aaatgagagg
1501 gacaaatttt ccccctgatg gacctgtgat gcagaagaaa actatgggat gggaggcctc
1561 caccgaaagg ctgtatccac gcgacggggt gctgaaagga gaaatccacc aggctctgaa
1621 gctgaaagat gggggacatt acctggtgga gttcaagaca atctacatgg ccaagaaacc
1681 tgtgcagctg ccaggctact attacgtgga cacaaaactg gatatcactt cacacaacga
1741 ggactacact attgtggagc agtatgaacg gagcgagggg agacaccatc tgttcctggg
1801 ccatgggact ggaagtaccg gctcagggtc tagtggaacc gcctcaagcg aggataacaa
1861 tatggctgtg atcaaagagt tcatgaggtt taaggtgcgc atggagggca gcatgaatgg
1921 gcacgaattt gagattgaag gagagggcga agggaggcct tacgagggca cacagactgc
1981 caagctgaaa gtgaccaagg gaggaccact gcctttcgct tgggatatcc tgtctcctca
2041 gtttatgtac ggaagtaagg cctatgtcaa gcatcccgct gacattcctg attacaagaa
2101 actgtctttc ccagagggct ttaagtggga gagagtgatg aattttgaag atggaggcct
2161 ggtgaccgtg acacaggact cctctctgca ggatggcact ctgatctaca aagtcaaaat
2221 gcgcggcacc aattttccac ccgatgggcc cgtgatgcag aagaaaacaa tggggtggga
2281 ggccagcact gaacggctgt atcctagaga cggagtgctg aagggcgaaa tccaccaggc
2341 cctgaagctg aaagacggcg gccactacct ggtggagttc aaaaccatct acatggccaa
2401 gaaaccagtg cagctgcccg gctattacta tgtggacacc aagctggata tcacatccca
2461 caatgaagac tacaccattg tggaacagta tgagaggtct gaaggacgcc accatctgtt
2521 tctgtacggc atggatgagc tgtataagta aagcggccgc gactctagat cataatcagc
2581 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
2641 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
2701 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
2761 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat
2821 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
2881 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
2941 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
3001 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
3061 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
3121 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
3181 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
3241 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
3301 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
3361 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
3421 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
3481 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
3541 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
3601 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
3661 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
3721 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
3781 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
3841 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
3901 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
3961 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
4021 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
4081 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
4141 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
4201 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
4261 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
4321 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
4381 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
4441 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
4501 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
4561 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
4621 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
4681 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
4741 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
4801 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
4861 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
4921 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
4981 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
5041 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
5101 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
5161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
5221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
5281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
5341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
5401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
5461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
5521 cag
//
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