pLX303

LOCUS       pLX303                9335 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLX303
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26. ():.
  PUBMED    21706014
REFERENCE   2  (bases 1 to 9335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        119..149
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             203..862
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(269..288)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1204..1509
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1388..1407
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1550..1674)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    1717..2305
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1770..1790)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2188..2199)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2377..2610
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2688..2809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2725..2744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2779..2798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2849..2984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2911..2930
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(3004..3023)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3005..3023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3033..3050)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3033..3049)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3042..3064)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3191..3646
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3278..3297)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3488..3509
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3672..3776
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3777..4637
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3995..4014)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4808..5396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5297..5316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5550..5567
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5684..5705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5720..5750
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5758..5774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5763..5785
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5782..5798
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5782..5798
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5817..5837
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5819..5837
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5865..6091
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6092..6272
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6319..6444
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6937..7170
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7355..7399
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7574..8074
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7993..8011
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8086..8481
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8541..8658
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8775..9078
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9079..9282
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9233..9253
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    9305..94
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
       61 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
      121 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
      181 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      241 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      301 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      361 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      421 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      481 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      541 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      601 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      661 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      721 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      781 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      841 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
      901 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
      961 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     1021 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     1081 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     1141 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     1201 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     1261 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     1321 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     1381 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     1441 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     1501 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     1561 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     1621 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtttagta
     1681 atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     1741 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     1801 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     1861 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     1921 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     1981 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2041 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     2101 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     2161 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2221 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     2281 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     2341 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     2401 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     2461 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2521 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2581 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     2641 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     2701 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2761 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     2821 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     2881 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2941 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     3001 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     3061 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3121 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3181 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3241 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3301 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3361 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3421 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3481 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3541 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3601 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     3661 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3721 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3781 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3841 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3901 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3961 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4021 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4081 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4141 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4201 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4261 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4321 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4381 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4441 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4501 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4561 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4621 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4681 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4741 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4801 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4861 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4921 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4981 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5041 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5101 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5161 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5221 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5281 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5341 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5401 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5461 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5521 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5581 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5641 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     5701 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     5761 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     5821 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     5881 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     5941 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     6001 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     6061 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     6121 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6181 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6241 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6301 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     6361 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     6421 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     6481 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     6541 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     6601 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     6661 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     6721 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     6781 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     6841 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     6901 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     6961 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     7021 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     7081 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     7141 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     7201 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     7261 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     7321 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     7381 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     7441 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     7501 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     7561 cccgagggga cccttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     7621 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     7681 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     7741 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     7801 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     7861 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     7921 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     7981 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     8041 cctcgttgac cgaatcaccg acctctctcc ccagggggta ccaccatggc caagcctttg
     8101 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     8161 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     8221 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     8281 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     8341 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     8401 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     8461 tctggttatg tgtgggaggg cctgcagctg cagtagtaag aattctagat cttgagacaa
     8521 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     8581 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     8641 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     8701 ctcgaggggg cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     8761 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     8821 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     8881 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     8941 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     9001 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     9061 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     9121 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     9181 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     9241 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg
     9301 atcaacaagt ttgtacaaaa aagctgaacg agaaa
//