| 质粒名称: | pAAVLINK-EF1a-HA-5'-IQSEC2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001356 |
| 产品用途: | 5' AAVLINK plasmid for IQSEC2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | IQSEC2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-IQSEC2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-IQSEC2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-IQSEC2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-IQSEC2质粒载体简介
pAAVLINK-EF1a-HA-5'-IQSEC2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-IQSEC2 5773 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5773)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5773
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..2913
/codon_start=1
/label=IQSEC2
/translation="MEAGSGPPGGPGSESPNRAVEYLLELNNIIESQQQLLETQRRRIE
ELEGQLDQLTQENRDLREESQLHRGELHRDPHGARDSPGRESQYQNLRETQFHHRELRE
SQFHQAARDVGYPNREGAYQNREAVYRDKERDASYPLQDTTGYTARERDVAQCHLHHEN
PALGRERGGREAGPAHPGREKEAGYSAAVGVGPRPPRERGQLSRGASRSSSPGAGGGHS
TSTSTSPATTLQRKSDGENSRTVSVEGDAPGSDLSTAVDSPGSQPPYRLSQLPPSSSHM
GGPPAGVGLPWAQRARLQPASVALRKQEEEEIKRSKALSDSYELSTDLQDKKVEMLERK
YGGSFLSRRAARTIQTAFRQYRMNKNFERLRSSASESRMSRRIILSNMRMQFSFEEYEK
AQNPAYFEGKPASLDEGAMAGARSHRLERGLPYGGSCGGGIDGGGSSVTTSGEFSNDIT
ELEDSFSKQVKSLAESIDEALNCHPSGPMSEEPGSAQLEKRESKEQQEDSSATSFSDLP
LYLDDTVPQQSPERLPSTEPPPQGRPEFWAPAPLPPVPPPVPSGTREDGSREEGTRRGP
GCLECRDFRLRAAHLPLLTIEPPSDSSVDLSDRSDRGSVHRQLVYEADGCSPHGTLKHK
GPPGRAPIPHRHYPAPEGPAPAPPGPLPPAPNSGTGPSGVAGGRRLGKCEAAGENSDGG
DNESLESSSNSNETINCSSGSSSRDSL"
misc_feature 1158..1163
/label=SDS
misc_feature 1671..1676
/label=SDS
misc_feature 1674..1679
/label=SDS
misc_feature 1677..1682
/label=SDS
misc_feature 2540..2545
/label=SDS
intron 2914..2954
/label=Splicing Donor
misc_feature 2958..2991
/label=lox2272-JT1571
repeat_region 3036..3176
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3036..3165
/label=AAV2 ITR
rep_origin 3251..3706
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3988..4092
/gene="bla"
/label=AmpR promoter
CDS 4093..4953
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5124..5712
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggaggcgggg tcggggcccc cgggcggccc
781 gggatccgag agcccaaatc gggccgtgga gtacctgctg gagctgaaca acatcatcga
841 gagccagcag cagctgctgg aaacccagcg gcggcgcatc gaggagctgg agggccagct
901 ggaccagctc acccaggaga accgcgacct gcgagaggag agccagctgc accgcgggga
961 gctgcaccgg gacccccacg gcgcgcggga tagcccgggc cgcgagagcc agtaccagaa
1021 cctgcgcgag acccagttcc accaccgcga gctgcgggag agccagttcc accaggcggc
1081 ccgggacgtg ggctacccga accgggaagg cgcctaccag aatcgggagg ctgtgtatcg
1141 ggacaaggag cgggacgcct cctacccgct ccaggacact accggttaca cagcccgcga
1201 gcgtgacgtg gcccagtgcc acctgcacca cgagaaccca gccctgggtc gcgagcgtgg
1261 cgggcgggag gccgggccgg cgcacccggg ccgcgagaag gaagcgggct attcggcggc
1321 ggtgggcgtg gggccgcggc caccgcggga gcggggccag ctgagccgtg gcgcatccag
1381 gagctccagt cccggcgccg gcggaggcca cagcaccagt accagcacca gcccggccac
1441 gaccctccag agaaaatctg atggtgagaa ttccagaaca gtcagtgtgg agggtgatgc
1501 cccaggcagt gacctgagca cagcggttga tagtcctggg agccaacccc cctaccggct
1561 gagccagctg cccccctcca gcagccacat ggggggcccc cctgctggag tgggccttcc
1621 ctgggctcag cgggcacgcc tccagccagc cagtgtcgcc ctgaggaagc aggaggagga
1681 ggagataaag cgctccaagg ccctatcgga cagctatgaa ctctccacag acctgcagga
1741 caagaaggtg gaaatgctgg agaggaagta tgggggctcc ttcctgagcc gcagggctgc
1801 caggaccatc cagacagcct tccgccagta ccgcatgaac aagaactttg agcggctacg
1861 cagctcagcc tcagagagcc gcatgtcccg ccgcatcatc ctttccaaca tgcggatgca
1921 gttctccttt gaggagtatg agaaggcaca gaaccccgcg tacttcgagg gcaagcctgc
1981 ctcgctggac gagggtgcca tggctggtgc ccggagccac cggcttgaac gggggctccc
2041 atatggaggc tcctgtggtg ggggcatcga tggtggtgga agctccgtca ccacatctgg
2101 agagttttct aatgacatca cagaacttga ggactccttc tccaaacagg taaagtctct
2161 ggctgaatcc atcgacgaag ccctgaactg ccacccgtca gggcccatgt ctgaggagcc
2221 agggtcagcc cagctggaga agcgggagtc aaaggaacag caagaggaca gctcagccac
2281 atccttcagt gatcttcccc tctacctgga tgacacagtt ccccaacaat cccctgagcg
2341 actgcccagc acagaacccc caccccaggg ccggcccgag ttctgggcgc cagcccctct
2401 cccgccagtt cctccaccag tgccatcagg aacccgggaa gacggtagcc gtgaggaagg
2461 cactcgcagg ggtcccgggt gcttggagtg ccgggatttc cggctgcggg ctgcccacct
2521 tcccctgctt accattgagc ctcctagtga cagctccgtg gacctgagtg accgctcaga
2581 tcgcggctct gtccaccgcc agctggtgta tgaggctgat ggctgcagcc cccatgggac
2641 cctgaagcac aaggggccac caggcagggc cccgatccca caccgccact acccagcccc
2701 tgaaggccca gccccagccc caccagggcc cctgccacca gcccccaaca gtggcactgg
2761 gcccagtggt gtggctgggg gtcggaggtt ggggaagtgc gaggcagcag gcgagaactc
2821 tgatggtgga gataacgaga gccttgagag ctccagcaat tccaatgaga ccatcaactg
2881 cagctccggc tcctcttctc gggacagtct aaggtaagta tcaaggttac aagacaggtt
2941 taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta ttaagttatg
3001 gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
3061 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
3121 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
3181 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
3241 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
3301 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
3361 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
3421 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
3481 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
3541 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
3601 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
3661 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
3721 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
3781 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
3841 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
3901 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
3961 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
4021 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
4081 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
4141 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
4201 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
4261 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
4321 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
4381 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
4441 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
4501 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
4561 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
4621 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
4681 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
4741 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
4801 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
4861 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
4921 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
4981 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
5041 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
5101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
5161 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
5221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
5281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
5341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
5401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
5461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
5521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
5581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
5641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
5701 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
5761 tttgctcaca tgt
//
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