| 质粒名称: | pISCIsp3-reRNA(crtYe) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002472 |
| 产品用途: | For gene editing in Corynebacterium glutamicum |
| 存储实验室: | 杨晟Sheng Yang Lab（CEMPS,CAS） |
|
载体骨架基本信息 |
|
| 载体名称: | pXMJ19 |
| 表达系统: | 细菌表达 |
| 启动子: |
PlacM, PJ23119
|
| 拷贝数: | 低拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | DH5alpha, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | ISClsp3, reRNA |
| 物种来源: | Clostridiales bacterium AM23 16LB |
| 密码子优化: | 大肠杆菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
One-Step Multi-fragment Assembly and Targeted Genomic Integration in Corynebacterium glutamicum
ACS Synthetic Biology，，2026/03/13/
Xiaoyu Wang, Siqi Yang, Xiaojie Zhou, Jiao Zhu, Fenghui Qian, Yu Jiang, Sheng Yang
|
买家导航
pISCIsp3-reRNA(crtYe)载体质粒基本信息
pISCIsp3-reRNA(crtYe)质粒图谱载体图谱和pISCIsp3-reRNA(crtYe)载体序列质粒序列多克隆位点信息
载体图谱.png)
pISCIsp3-reRNA(crtYe)质粒载体简介
pISCIsp3-reRNA(crtYe)质粒序列载体序列
LOCUS pISCIsp3-reRNA(crtYe) 7443 bp DNA circular SYN 20-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7443)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7443
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 4584..5747
/strain="ATCC 13032"
/mol_type="genomic DNA"
/db_xref="taxon:196627"
/organism="Corynebacterium glutamicum ATCC 13032"
source 6068..6087
/strain="DSM 20300 = ATCC 13032"
/mol_type="genomic DNA"
/label=IS fingerprint type: 4-5
/note="IS fingerprint type: 4-5"
/db_xref="taxon:196627"
/type_material="type strain of Corynebacterium glutamicum"
/organism="Corynebacterium glutamicum ATCC 13032"
source 6274..6308
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(860..1912)
/codon_start=1
/gene="''pBL1"
/label=''pBL1
/translation="EVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAG
IKTAYSSMVKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDEL
KAFEDSMFSRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGS
ATKTASKGSYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRAL
GIDYIDADVRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLD
CVDKAKDVAAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFA
GNEQTILRTH"
CDS complement(1913..3330)
/codon_start=1
/gene="pBL1'"
/label=pBL1'
/translation="IRGRSLVPLSDFWHRRTPVNCVVPGVADFCETSRAIP*LR*KHHE
TLGKHP*NTH*GSRAASSSRACIWAVIWSLACESVS*VACSMHSNVTSFTGSRWAKRTS
GLDIVFLVVGGGELF*PLGKRGDHELLEVFTVLHACALHVLT*CQRNACGDHDGLSLCL
RF*CFDGGLCLRLLRL*CLLSFL*LLF*LCLGCHALRL**LSCDMSCACVANIVLQLIH
YVQCSCY*SYILIFT*SACSACTCSHVVLMCKTCTCRLLGVQGAEPPCPCGVWGLPRRY
RQ*APGHLVADTPPRYRTRNPPMSMQIFNIEYG*AGTHSQARRPPNTTKN**SLDKTNP
RASYQLICTGAT*PEGVSIDNHSTS*DNGHNTRTNSHCATPHNIGSR*H*VTLK*K*TP
LRNRRSMRVLRSLALGRGVGKTSCTRSPIVRLWRGAIGGAGTKLLRCPGRLTVLRSLRV
CKTLTLAGGHLWLN"
CDS 1927
/codon_start=1
/gene="muC-T"
/label=muC-T
/translation=""
CDS 3697..4491
/codon_start=1
/transl_table=11
/gene="aph(3')-IIa"
/product="Aph(3')-IIa"
/label=aph
/label=aph(3')-IIa
/note="aminoglycoside phosphotransferase"
/db_xref="GI:29164938"
/protein_id="AAO65197.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 4490..4548
/label=plac-cpf1-F
misc_feature 4526..4583
/label=plac
CDS 4584..5747
/codon_start=1
/label=ISClsp3
/translation="MRKLLKSFKTEINPTEEQKTKIRKTIGTCRFIYNFYLAHNKKLHE
DGKKFMSSNKFRIWLNNEYLPQHPEYSWIKEAYSKAVTQSVNNGQTAFTRFFNHESAFP
NFKKKGKSDVKMYFVKNNPKDCHCERHRIKIPSLGWVRIKEKGYIPTTKDGYVIKSGSV
SIKADRYYVSVLVEVSDNKTINHFDEGIGIDLGLKDFAIVSNGRTYQNINKSAKLKKLE
RQLIREQRCLSRKYENLKKGDATQRANIQKQKLKVQKLHHRIDNIRTDYINKTIAEMVK
IKPSYITIEDLNVKGMMKNRHLSKAVASQKFYEFRAKLLAKCNESGIELRVVDRWYPSS
KTCHCCGSIKKDLKLSDRIFKCRCGYIEDRDFNAALNLRDAETYSIA"
CDS complement(5773..5995)
/codon_start=2
/gene="'rrnBTEM"
/label='rrnBTEM
/translation="NKTKGSVERLGLSFYLLFVGERSPE*DKSAGSGFERCEATARRVA
GRTPAINCQASN*AEGHPDGWPFCVSTNS"
exon 6000..6067
/label=HDV
primer_bind complement(6048..6104)
/label=1477n-crtYen20-R
primer_bind 6053..6111
/label=1476n-crtYen20-10-F
gene 6068..6087
/gene="crtYe"
/locus_tag="cg0719"
/label=crtYe
CDS 6068..6087
/codon_start=1
/transl_table=11
/gene="crtYe"
/locus_tag="cg0719"
/product="C50 CAROTENOID EPSILON CYCLASE"
/label=crtYe
/db_xref="GOA:Q6M7E2"
/db_xref="InterPro:IPR017825"
/db_xref="UniProtKB/TrEMBL:Q6M7E2"
/protein_id="CAF19329.1"
/translation="FIYLGT"
misc_feature 6068..6087
/label=crtYe-AGGAG
misc_feature 6068..6087
/label=del
misc_feature 6071..6087
/label=crtYe-ngg
misc_feature 6088..6273
/label=cs
misc_feature complement(6274..6308)
/label=pJ23119
CDS complement(6426..7376)
/codon_start=1
/gene="repA101"
/product="repA101 protein"
/label=repA101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
CDS 6512
/codon_start=1
/gene="ts"
/label=ts
/translation=""
ORIGIN
1 ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa
61 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
121 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
181 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
241 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
301 accctcgcaa gctcggttgc ggccgcaatc gggcaaatcg ctgaatattc cttttgtctc
361 cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg
421 ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt catgcaagga
481 aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt atggcgggtc
541 tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct gattttccag
601 tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc acccagtaag
661 gcagcggtat catcaaggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt acgctccggg acgccaacaa
781 caagacccat catagtttgc ccccgcgaca ttgaccataa attcatcgca caaaatatcg
841 aacggggttt atgccgcttt tagtgggtgc gaagaatagt ctgctcatta cccgcgaaca
901 ccgccgcatt cagatcacgc ttagtagcgt ccccatgagt aggcagaacc gcgtccaagt
961 ccacatcatc cataacgatc atgcacgggg tggaatccac acccagactt gccagcacct
1021 cattagcgac acgttgcgca gcggccacgt ccttagcctt atccacgcaa tcgagaacgt
1081 actgcctaac cgcgaaatca gactgaatca gtttccaatc atcgggcttc accaaagcaa
1141 cagcaacgcg ggttgattcg acccgttccg gtgcttccag accggcgagc ttgtacagtt
1201 cttcttccat ttcacgacgt acatcagcgt ctatgtaatc aatgcccaaa gcacgcttag
1261 ccccacgtga ccaggacgaa cgcaggtttt tagaaccaac ctcatactca cgccaccgag
1321 ccaccaaaac agcgtccata tcctcgccgg cgtcgctttg atcggccaac atatccaaca
1381 tctgaaacgg cgtgtacgac cccttagacg cggttttagt agcggagcca gtcagttcct
1441 gagacatgcc cttagcgagg taggttgcca ttttcgcagc gtctccaccc caggtagaca
1501 cctgatcaag tttgaccccg tgctcacgca gtggcgcgtc cataccggcc ttaaccacac
1561 cagcagacca gcgggaaaac atggaatcct caaacgcctt gagttcatcg tcagacagtg
1621 gacgatccaa gaacaacagc atgttgcggt gcaagtgcca accgttcgcc caagagtctg
1681 tgacctcata gtcactatag gtgtgctcca ccccgtaccg tgcacgttct ttcttccact
1741 gagatgtttt caccatcgaa gagtacgcag tcttaatacc cgcttcaacc tgcgcaaatg
1801 actgtgagcg gttgtgtcga acagtgccca caaacatcat gagcgcgcca cccgccgcca
1861 agtgattctt agtagcaata gccagctcaa tgcggcgttc gcccatgact tccaattcag
1921 ccagagatga cccccagcga gagtgagagt tttgcagacc ctcaaactgc gaagcaccgt
1981 tagacgacca ggacaccgca acagcttcgt ccctgcgcca cctatggcac cccgccagag
2041 ccttactatt ggtgatcttg tacatgacgt tttgcctacg ccacgcccta gcgcgagtga
2101 ccttagaacc ctcattgacc tgcggttcct tagaggtgtt cacttctatt tcagtgttac
2161 tcagtgttac ctagacccga tgttgtgcgg ggttgcgcag tgcgagtttg tgcgggtgtt
2221 gtgcccgttg tcttagctag tgctatggtt gtcaattgaa accccttcgg gttatgtggc
2281 ccccgtgcat atgagttggt agctcgcacg ggggtttgtc ttgtctaggg actattaatt
2341 tttagtggtg tttggtggcc gcctagcttg gctatgcgtg ccagcttacc cgtactcaat
2401 gttaaagatt tgcatcgaca tgggagggtt acgtgtccga tacctagggg gggtatccgc
2461 gactaggtgc cccggtgctc actgtctgta ccggcggggc aagccccaca ccccgcatgg
2521 acagggtggc tccgccccct gcacccccag caatctgcat gtacatgttt tacacattag
2581 cacgacatga ctgcatgtgc atgcactgca tgcagactag gtaaatatga gtatgtacga
2641 ctagtaacag gagcactgca cataatgaat gagttgcagg acaatgtttg ctacgcatgc
2701 gcatgacata tcgcaggaaa gctactagag tcttaaagca tggcaaccaa ggcacagcta
2761 gaacagcaac tacaagaagc tcaacaggca ctacaggcgc agcaagcgca ggcacaagcc
2821 accatcgaag cactagaagc gcaggcaaag gctaagcccg tcgtggtcac cgcacgcgtt
2881 cctttggcac tacgtgagga catgaagcgc gcaggcatgc agaacggtga aaacctccaa
2941 gagttcatga tcgccgcgtt taccgagcgg ctagaaaagc tcaccaccac cgacaacgag
3001 gaaaacaatg tctaacccac tagttctctt tgcccaccgt gacccggtaa atgacgtgac
3061 gttcgagtgc attgagcacg ccacctacga cacactttca cacgctaaag accagatcac
3121 cgcccaaatg caagccctag acgaagaagc cgccctactg ccctaatggg tgtttcatgg
3181 gtgtttccct agtgtttcat ggtgttttca cctaagctag ggaattgcgc gagaagtctc
3241 gcaaaaatca gcaacccccg gaaccacaca gttcacgggg gttcttctat gccagaaatc
3301 agaaagggga accagtgaac gaccccgaat ggctggatga tcctccttgg gccacgccca
3361 cccggcgatc atcgaggcgg tcaccggaag cggaacacgt agaaagccag tccgcagaaa
3421 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
3481 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
3541 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
3601 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
3661 agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
3721 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
3781 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
3841 gtcaagaccg acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg
3901 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
3961 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
4021 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
4081 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
4141 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
4201 gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccat
4261 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
4321 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
4381 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
4441 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
4501 tggggttcgc ggaatcatga cctgagctgt ttacaattaa tcatcgtgtg gtaccatgtg
4561 tggaattgga aaggacttga acgatgcgca aactgctgaa aagcttcaaa accgaaatca
4621 acccgaccga agaacagaaa accaaaatcc gcaaaaccat cggcacctgc cgcttcatct
4681 acaacttcta cctggcccac aacaaaaaac tgcacgaaga tggcaaaaaa ttcatgagca
4741 gcaacaaatt ccgcatctgg ctgaacaacg aatacctgcc gcagcacccg gaatacagct
4801 ggatcaaaga agcctacagc aaagccgtga cacagagcgt gaacaacggt cagaccgcct
4861 tcacccgctt cttcaaccac gaaagcgcct tcccgaactt caaaaagaaa ggcaaaagcg
4921 atgtgaaaat gtacttcgtg aaaaacaacc cgaaagattg ccactgcgaa cgccaccgca
4981 tcaaaatccc gagcctgggc tgggtgcgca tcaaagaaaa aggctacatc ccgaccacca
5041 aagatggcta cgtgatcaaa agcggcagcg tgagcatcaa agccgatcgc tactacgtga
5101 gcgtgctggt ggaagtgagc gataacaaaa ccatcaacca cttcgatgaa ggcatcggca
5161 tcgatctggg cctgaaagat ttcgccatcg tgagcaacgg ccgcacctat cagaatatca
5221 acaaaagcgc caaactgaaa aaactggaac gtcagctgat ccgcgaacag cgctgcctga
5281 gccgcaaata cgaaaacctg aaaaaaggcg atgccacaca gcgcgccaac attcagaaac
5341 agaaactgaa agtgcagaaa ctgcaccacc gcatcgataa catccgcacc gattacatca
5401 acaaaacaat tgccgaaatg gtgaaaatca aaccgagcta catcaccatc gaagatctga
5461 acgtgaaagg catgatgaaa aaccgccacc tgtccaaagc cgtggcgagc caaaaattct
5521 acgaattccg cgccaaactg ctggccaaat gcaacgaaag cggcatcgaa ctgcgcgtgg
5581 tggatcgctg gtacccgagc agcaaaacct gccactgctg cggcagcatc aaaaaagatc
5641 tgaaactgag cgatcgcatc ttcaaatgcc gctgcggcta catcgaagat cgcgatttca
5701 acgccgccct gaacctgcgc gatgccgaaa cctacagcat cgcctaagtc gacctgcagg
5761 catgcaagct taagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
5821 ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
5881 ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
5941 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttaaatg
6001 tcccattcgc catgccgaag catgttgccc agccggcgcc agcgaggagg ctgggaccat
6061 gccggcccct ttatttatct aggtactctg ccactcaaaa tgtgattaaa tatacccaca
6121 tcgagataat gtaagttctc gcgaacatta cttactgctt cattgtgtat gtttttgatt
6181 gaccgtagtc atatctactc acaagttcat gtacactcca cagtcgtaaa ttcccgaaat
6241 agccttcggt acatacttac atttgcgttt taaggatcca ttatacctag gactgagcta
6301 gctgtcaatc tttcagcctg gcggtgtaat gcaccgccaa cttgaaagtt aacaggtgaa
6361 cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca agagccataa
6421 gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc
6481 gtgagccatg agaacgaacc attgagatca tgcttacttt gcatgtcact caaaaatttt
6541 gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt
6601 ccgttacgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt
6661 atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa
6721 atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg
6781 tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag
6841 ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg
6901 tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg
6961 ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga
7021 taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag
7081 tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc
7141 gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct actgatgttc
7201 atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt
7261 tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt
7321 aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat
7381 ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa
7441 tta
//
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