V5-LOV-Turbo-ERM

LOCUS      V5-LOV-Turbo-ERM       9465 bp    DNA     circular SYN 14-APR-2025
DEFINITION  expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Lee SY, Cheah JS, Zhao B, Xu C, Roh H, Kim CK, Cho KF, Udeshi ND, 
            Carr SA, Ting AY
  TITLE     Engineered allostery in light-regulated LOV-Turbo enables precise 
            spatiotemporal control of proximity labeling in living cells.
  JOURNAL   Nat Methods. 2023 May 15. doi: 10.1038/s41592-023-01880-5.
  PUBMED    37188954
REFERENCE   2  (bases 1 to 9465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 May 15. doi: 10.1038/s41592-023-01880-5."
COMMENT     Sequence Label: V5-LOV-Turbo-ERM
FEATURES             Location/Qualifiers
     source          1..9465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1752..1869
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1963..1981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1999..2017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2035..2053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2107..2125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2143..2161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2206..2230
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2308..2317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2317..2358
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3775..4062
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="PGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGR
                     TTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     misc_feature    4076..4664
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4129..4149)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4547..4558)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4684..5194
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5113..5131
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5204..5947
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             5957..6013
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6014..6610
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6507..6527
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6709..6942
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7014..7135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7051..7070)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7105..7124
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7175..7310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7310..7381
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7382..7450,7451..8242)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7600..7619)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8413..9001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8902..8921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9155..9172
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9289..9310
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9325..9330,9331..9348,9349..9355)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9363..9379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9368..9390
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9387..9403
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9387..9403
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9422..9442
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9424..9442
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta
     1741 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1801 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1861 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg
     1921 ttttactccc tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag
     1981 aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta
     2041 tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca
     2101 gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag
     2161 aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact ttccgtacca
     2281 cttcctaccc tcgtaaatag agctagcgcc accatgggca agcccatccc caaccccctg
     2341 ctgggcctgg acagcaccgg taccaaagac aacacagtgc ctctgaagct gatcgccctc
     2401 ctggccaatg gagagttcca cagcggcgag cagctgggcg aaaccctggg catgagcaga
     2461 gccgctatca acaagcacat ccagaccctt cgcgactggg gcgtcgacgt gttcagcgtg
     2521 cccggcaagg gctacagcct gcctgagcct atccccctgc tgaacgccaa gcagattcag
     2581 ggccagctgg aatttagagc caccaccctg gaaagaatcg agaagtcttt tgtgatcacc
     2641 gaccccagac tgcctgataa tcctatcatc ttcgtgtctg atagcttcct gcagctcacc
     2701 gagtattctc gggaagagat cctcggaaga aattgcagat tcctgcaggg cccagagaca
     2761 gatagagcca cagtgcggaa gatcagagat gccatcgaca accagacaga ggtgaccgtg
     2821 cagctgatca actacaccaa gtccggcaaa aagttctgga acgttttcca tctccaacct
     2881 atgagagact acaagggcga cgtgcagtac ttcatcggag ttcagctgga cggcaccgag
     2941 agactgcacg gcgccgcaga gcgggaagct gtgtgcctgg tcaagaagac cgccttccag
     3001 atcgccgtgg ctgtgctgcc cgtggtggac agcacaaacc agtacctgct ggacagaatc
     3061 ggcgagctga agtccggcga cgcttgtatc gccgaatacc agcaggccgg cagaggcagc
     3121 agaggccgga aatggttcag ccccttcggc gccaacctgt acctgagcat gttttggcgg
     3181 ctgaaaagag gccctgccgc cacaggcctg ggccccgtga taggaatcgt gatggccgag
     3241 gctctgagga agctgggtgt ggacaaagtg cgggtgaagt ggcctaacga cctgtacctg
     3301 caggacagaa agctggccgg catcctggtg gaactggctg gcatcaccgg cgacgccgct
     3361 cagattgtga tcggcgctgg catcaatgtg gccatgagac gcgtggaaga gagcgtggtc
     3421 aaccagggct ggatcacact gcaagaggcc ggtgtgaacc tggataggaa caccctggcc
     3481 gccaccctga tcagagagct gagagccgct ctggaattgt ttgagcagga gggcctggcc
     3541 ccttacctgc cacggtggga gaaactggat aacttcatca acaggcctgt gaagctgatc
     3601 atcggcgata aggaaatcct gggaatcagc cggggcatcg acaagcaggg agccctgctg
     3661 ctggaacaag atggagtgat taagccttgg atgggcggag aaatcagcct gcggtccgcc
     3721 gagaagggat ccggtggagg cgggtctgga ggcgggggta gtaacagccg cgtgcctggt
     3781 ccgaccccca gtggcactaa cgtgggatcc tcagggcgct ctcccagcaa agcagtggcc
     3841 gcccgggcgg cgggatccac tgtccggcag aggaaaaatg ccagctgtgg gacaaggagt
     3901 gcaggccgca caacctcggc aggcaccggg gggatgtggc gattctacac agaagattca
     3961 cctgggctca aagttggccc tgttccagta ttggttatga gtcttctgtt catcgcttct
     4021 gtatttatgt tgcacatttg gggcaagtac actcgttcgt agtaaggccg aattcaatca
     4081 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4141 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4201 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4261 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4321 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4381 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4441 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     4501 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4561 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4621 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgtct gcagatatca
     4681 tccggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     4741 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     4801 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     4861 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     4921 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     4981 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     5041 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     5101 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     5161 cctcgttgac cgaatcaccg acctctctcc ccagcaattc accatgtcta gactggacaa
     5221 gagcaaagtc ataaactctg ctctggaatt actcaatgga gtcggtatcg aaggcctgac
     5281 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     5341 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     5401 ctcctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata
     5461 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     5521 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     5581 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     5641 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     5701 gcccccactt ctgaaacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     5761 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     5821 cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     5881 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     5941 ccccggggga agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga
     6001 gaaccctgga cctaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6061 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6121 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6181 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     6241 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6301 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6361 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     6421 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     6481 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     6541 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6601 cggtgcctaa ctgtttcgcc tcggcgtccg gactagaggt acctttaaga ccaatgactt
     6661 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6721 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     6781 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6841 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6901 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6961 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7141 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7201 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7261 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tttcaaatat
     7321 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7381 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7441 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7501 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7561 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7621 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7681 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7741 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7801 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7861 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7921 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7981 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8041 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8101 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8161 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8221 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8281 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8341 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8401 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8461 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8521 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8581 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8641 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8701 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8761 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8821 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8881 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8941 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9001 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9061 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9181 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9241 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9301 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9361 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     9421 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//