pCytERM-mNeptune2

LOCUS       pCytERM-mNeptune2          4827 bp DNA     circular SYN 22-OCT-2024
DEFINITION  TSN20241013-0571-00010_749_20241014-BAN4_H02_H02_i20.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        40..397
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      248..383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             432..1226
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    1458..1505
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1834..2422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        2597..2900
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2901..3104
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3156..3242
                     /codon_start=1
                     /label=cytochrome p450
                     /translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
     regulatory      3288..3297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3294..4028
                     /codon_start=1
                     /product="far-red fluorescent protein with spectra similar 
                     to those of mNeptune but improved brightness"
                     /label=mNeptune2
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTG
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     polyA_signal    4151..4272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4279..4734)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        join(4761..4827,1..38)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
       61 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      121 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
      181 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
      241 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
      301 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
      361 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
      421 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
      481 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
      541 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
      601 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
      661 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
      721 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
      781 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
      841 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
      901 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
      961 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     1021 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     1081 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     1141 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     1201 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     1261 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1321 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1381 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     1441 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     1501 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     1561 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     1621 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     1681 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1741 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1801 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1861 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1921 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1981 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2041 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2101 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2161 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2221 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2281 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2341 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2401 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2461 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2521 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     2581 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2641 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2701 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2761 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2821 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2881 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2941 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     3001 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     3061 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
     3121 agatccgcta gcgctaccgg actcagatct ccaccatgga tcctgtggtg gtgctggggc
     3181 tctgtctctc ctgtttgctt ctcctttcac tctggaaaca gagctatggg ggagggaagc
     3241 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtgt
     3301 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     3361 tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     3421 agaccggcag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     3481 ctacctgctt catgtacggc agcaagacct tcatcaacca cacccagggc atccccgatt
     3541 tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     3601 ggggcgtgct taccgttacc caggacacca gcctccagga cggctgcttg atctacaacg
     3661 tcaagctcag aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
     3721 gctgggaggc cagtaccgag accctgtacc ccgctgacgg cggcctggaa ggcagatgcg
     3781 acatggccct gaagctcgtg ggcgggggcc acctgcactg caacctgaag accacataca
     3841 gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactttgtg gaccgcagac
     3901 tggaaagaat caaggaggcc gacaatgaga cctacgtcga gcagcacgag gtggctgtgg
     3961 ccagatactg cgacctccct agcaaactgg ggcacaaact taatggcatg gacgagctgt
     4021 acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     4081 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     4141 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4261 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     4321 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     4381 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     4441 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     4501 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     4561 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     4621 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     4681 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     4741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4801 atatgtatcc gctcatgaga caataac
//