| 质粒名称: | pAAVLINK-EF1alpha-5'-COL4A5 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001895 |
| 产品用途: | 5' AAVLINK plasmid for COL4A5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL4A5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-5'-COL4A5
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-5'-COL4A5载体质粒基本信息
pAAVLINK-EF1alpha-5'-COL4A5质粒图谱载体图谱和pAAVLINK-EF1alpha-5'-COL4A5载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-5'-COL4A5质粒载体简介
pAAVLINK-EF1alpha-5'-COL4A5质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-5'-COL4A5 6686 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6686)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6686
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..3826
/codon_start=1
/gene="COL4A5"
/gene_synonym="ASLN; ATS; ATS1; CA54"
/product="collagen alpha-5(IV) chain isoform 2 precursor"
/label=COL4A5
/note="isoform 2 precursor is encoded by transcript
variant 2; collagen of basement membrane, alpha-5 chain;
collagen IV, alpha-5 polypeptide; collagen alpha-5(IV)
chain; dA149D17.3; collagen, type IV, alpha 5; dA24A23.1"
/db_xref="CCDS:CCDS35366.1"
/db_xref="GeneID:1287"
/db_xref="HGNC:HGNC:2207"
/db_xref="MIM:303630"
/protein_id="NP_203699.1"
/translation="MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKG
EKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTP
GLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIM
SSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNF
QGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPP
GGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPG
PPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPP
GFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGP
PGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEK
GQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPG
TPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKG
IQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGI
PGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGF
ALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGP
PGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAP
GPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQ
PGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGD
PGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQ"
misc_feature 992..997
/label=SDS
misc_feature 1244..1249
/label=SDS
misc_feature 1325..1330
/label=SDS
misc_feature 1514..1519
/label=SDS
misc_feature 1733..1738
/label=SDS
misc_feature 1895..1900
/label=SDS
misc_feature 1904..1909
/label=SDS
misc_feature 1973..1978
/label=SDS
misc_feature 2018..2023
/label=SDS
misc_feature 2045..2050
/label=SDS
misc_feature 2924..2929
/label=SDS
misc_feature 3146..3151
/label=SDS
misc_feature 3260..3265
/label=SDS
misc_feature 3563..3568
/label=SDS
misc_feature 3776..3781
/label=SDS
intron 3827..3867
/label=Splicing Donor
misc_feature 3871..3904
/label=lox2272-JT1571
repeat_region 3949..4089
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3949..4078
/label=AAV2 ITR
repeat_region 3950..4090
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4164..4619
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4901..5005
/gene="bla"
/label=AmpR promoter
CDS 5006..5866
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6037..6625
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgaaact
721 gcgtggagtc agcctggctg ccggcttgtt cttactggcc ctgagtcttt gggggcagcc
781 tgcagaggct gcggcttgct atgggtgttc tccaggatca aagtgtgact gcagtggcat
841 aaaaggggaa aagggagaga gagggtttcc aggtttggaa ggacacccag gattgcctgg
901 atttccaggt ccagaagggc ctccggggcc tcggggacaa aagggtgatg atggaattcc
961 agggccacca ggaccaaaag gaatcagagg tcctcctgga cttcctggat ttccagggac
1021 accaggtctt cctggaatgc caggccacga tggggcccca ggacctcaag gtattcccgg
1081 atgcaatgga accaagggag aacgtggatt tccaggcagt cccggttttc ctggtttaca
1141 gggtcctcca ggaccccctg ggatcccagg tatgaagggt gaaccaggta gtataattat
1201 gtcatcactg ccaggaccaa agggtaatcc aggatatcca ggtcctcctg gaatacaagg
1261 cctacctggt cccactggta taccagggcc aattggtccc ccaggaccac caggtttgat
1321 gggccctcct ggtccaccag gacttccagg acctaagggg aatatgggct taaatttcca
1381 gggacccaaa ggtgaaaaag gtgagcaagg tcttcagggc ccacctgggc cacctgggca
1441 gatcagtgaa cagaaaagac caattgatgt agagtttcag aaaggagatc agggacttcc
1501 tggtgaccga gggcctcctg gacctccagg gatacgtggt cctccaggtc ccccaggtgg
1561 tgagaaaggt gagaagggtg agcaaggaga gccaggcaaa agaggtaaac caggcaaaga
1621 tggagaaaat ggccaaccag gaattcctgg tttgcctggt gatcctggtt accctggtga
1681 acccggaagg gatggtgaaa agggccaaaa aggtgacact ggcccacctg gacctcctgg
1741 acttgtaatt cctagacctg ggactggtat aactatagga gaaaaaggaa acattgggtt
1801 gcctgggttg cctggagaaa aaggagagcg aggatttcct ggaatacagg gtccacctgg
1861 ccttcctgga cctccagggg ctgcagttat gggtcctcct ggccctcctg gatttcctgg
1921 agaaaggggt cagaaaggtg atgaaggacc acctggaatt tccattcctg gacctcctgg
1981 acttgacgga cagcctgggg ctcctgggct tccagggcct cctggccctg ctggccctca
2041 cattcctcct agtgatgaga tatgtgaacc aggccctcca ggccccccag gatctccagg
2101 tgataaagga ctccaaggag aacaaggagt gaaaggtgac aaaggtgaca cttgcttcaa
2161 ctgcattgga actggtattt cagggcctcc aggtcaacct ggtttgccag gtctcccagg
2221 tcctccagga tctcttggtt tccctggaca gaaaggggaa aaaggacaag ctggtgcaac
2281 tggtcccaaa ggattaccag gcattccagg agctccaggt gctccaggct ttcctggatc
2341 taaaggtgaa cctggtgata tcctcacttt tccaggaatg aagggtgaca aaggagagtt
2401 gggttcccct ggagctccag ggcttcctgg tttacctggc actcctggac aggatggatt
2461 gccagggctt cctggcccga aaggagagcc tggtggaatt acttttaagg gtgaaagagg
2521 tccccctggg aacccaggtt taccaggcct cccagggaat atagggccta tgggtccccc
2581 tggtttcggc cctccaggcc cagtaggtga aaaaggcata caaggtgtgg caggaaatcc
2641 aggccagcca ggaataccag gtcctaaagg ggatccaggt cagactataa cccagccggg
2701 gaagcctggc ttgcctggta acccaggcag agatggtgat gtaggtcttc caggtgaccc
2761 tggacttcca gggcaaccag gcttgccagg gatacctggt agcaaaggag aaccaggtat
2821 ccctggaatt gggcttcctg gaccacctgg tcccaaaggc tttcctggaa ttccaggacc
2881 tccaggagca cctgggacac ctggaagaat tggtctagaa ggccctcctg ggccacccgg
2941 ctttccagga ccaaagggtg aaccaggatt tgcattacct gggccacctg ggccaccagg
3001 acttccaggt ttcaaaggag cacttggtcc aaaaggtgat cgtggtttcc caggacctcc
3061 gggtcctcca ggacgcactg gcttagatgg gctccctgga ccaaaaggtg atgttggacc
3121 aaatggacaa cctggaccaa tgggacctcc tgggctgcca ggaataggtg ttcagggacc
3181 accaggacca ccagggattc ctgggccaat aggtcaacct ggtttacatg gaataccagg
3241 agagaagggg gatccaggac ctcctggact tgatgttcca ggacccccag gtgaaagagg
3301 cagtccaggg atccccggag cacctggtcc tataggacct ccaggatcac cagggcttcc
3361 aggaaaagca ggtgcctctg gatttccagg taccaaaggt gaaatgggta tgatgggacc
3421 tccaggccca ccaggacctt tgggaattcc tggcaggagt ggtgtacctg gtcttaaagg
3481 tgatgatggc ttgcagggtc agccaggact tcctggccct acaggagaaa aaggtagtaa
3541 aggagagcct ggccttccag gccctcctgg accaatggat ccaaatcttc tgggctcaaa
3601 aggagagaag ggggaacctg gcttaccagg tatacctgga gtttcagggc caaaaggtta
3661 tcagggtttg cctggagacc cagggcaacc tggactgagt ggacaacctg gattaccagg
3721 accaccaggt cccaaaggta accctggtct ccctggacag ccaggtctta taggacctcc
3781 tggacttaaa ggaaccatcg gtgatatggg ttttccaggg cctcaggtaa gtatcaaggt
3841 tacaagacag gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag
3901 ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
3961 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
4021 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
4081 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
4141 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
4201 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
4261 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
4321 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
4381 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
4441 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
4501 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
4561 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
4621 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
4681 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
4741 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
4801 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
4861 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
4921 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
4981 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
5041 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
5101 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
5161 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
5221 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
5281 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
5341 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
5401 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
5461 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
5521 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
5581 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
5641 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
5701 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
5761 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
5821 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
5881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
5941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
6001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
6061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
6121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
6181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
6241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
6301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
6361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
6421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
6481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
6541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
6601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
6661 cttttgctgg ccttttgctc acatgt
//
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pAAVLINK-EF1alpha-5'-COL4A5质粒载体应用举例
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