AAV-PCaMKII-Mt-mScarlet

LOCUS       AAV-PCaMKII-Mt-mScarlet               5982 bp DNA     circular SYN 02-APR-2026
DEFINITION  Forebrain principle neuron specific expression of GCaMP6f Ca sensor 
            and physically separate nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    2480-AAV-PCaMKII-Mt-mScarlet
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5982)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
FEATURES             Location/Qualifiers
     source          1..5982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        164..1452
                     /label=mouse aCaMKII promoter full length
     primer_bind     164..190
                     /label=new F2
     primer_bind     1309..1326
                     /label=Primer 2
     primer_bind     1335..1350
                     /label=Primer 1
     primer_bind     1372..1390
                     /label=AAV-aCaMKKII_F
     regulatory      1474..1483
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1480..1659
                     /codon_start=1
                     /label=Mito-advanced Sequence
                     /translation="MAQRLLLRRFLASVISRKPSQGQWPPLTSRALQTPQCSPGGLTVT
                     PNPARTIYTTRISLT"
     primer_bind     1647..1692
                     /label=mNeoGreen_F_AgeI
     CDS             1675..2370
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     2076..2095
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2351..2396)
                     /label=mNeoGreen_SpeI_R
     misc_feature    2401..2989
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2454..2474)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2872..2883)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3022..3133
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3245..3385
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3460..3915
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3757..3778
                     /label=F1ori-F
     primer_bind     complement(3932..3951)
                     /label=pRS-marker
     primer_bind     4051..4073
                     /label=pGEX 3'
     primer_bind     complement(4111..4129)
                     /label=pBRforEco
     primer_bind     complement(4171..4188)
                     /label=pQE promoter
     promoter        4197..4301
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4302..5162
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4520..4539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5333..5921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5822..5841
                     /label=pBR322ori-F
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaat taacattatg gccttaggtc
      181 acttcatctc catggggttc ttcttctgat tttctagaaa atgagatggg ggtgcagaga
      241 gcttcctcag tgacctgccc agggtcacat cagaaatgtc agagctagaa cttgaactca
      301 gattactaat cttaaattcc atgccttggg ggcatgcaag tacgatatac agaaggagtg
      361 aactcattag ggcagatgac caatgagttt aggaaagaag agtccagggc agggtacatc
      421 tacaccaccc gcccagccct gggtgagtcc agccacgttc acctcattat agttgcctct
      481 ctccagtcct accttgacgg gaagcacaag cagaaactgg gacaggagcc ccaggagacc
      541 aaatcttcat ggtccctctg ggaggatggg tggggagagc tgtggcagag gcctcaggag
      601 gggccctgct gctcagtggt gacagatagg ggtgagaaag cagacagagt cattccgtca
      661 gcattctggg tctgtttggt acttcttctc acgctaaggt ggcggtgtga tatgcacaat
      721 ggctaaaaag cagggagagc tggaaagaaa caaggacaga gacagaggcc aagtcaacca
      781 gaccaattcc cagaggaagc aaagaaacca ttacagagac tacaaggggg aagggaagga
      841 gagatgaatt agcttcccct gtaaacctta gaacccagct gttgccaggg caacggggca
      901 atacctgtct cttcagagga gatgaagttg ccagggtaac tacatcctgt ctttctcaag
      961 gaccatccca gaatgtggca cccactagcc gttaccatag caactgcctc tttgccccac
     1021 ttaatcccat cccgtctgtt aaaagggccc tatagttgga ggtgggggag gtaggaagag
     1081 cgatgatcac ttgtggacta agtttgttcg catccccttc tccaaccccc tcagtacatc
     1141 accctggggg aacagggtcc acttgctcct gggcccacac agtcctgcag tattgtgtat
     1201 ataaggccag ggcaaagagg agcaggtttt aaagtgaaag gcaggcaggt gttggggagg
     1261 cagttaccgg ggcaacggga acagggcgtt tcggaggtgg ttgccatggg gacctggatg
     1321 ctgacgaagg ctcgcgaggc tgtgagcagc cacagtgccc tgctcagaag ccccaagctc
     1381 gtcagtcaag ccggttctcc gtttgcactc aggagcacgg gcaggcgagt ggcccctagt
     1441 tctgggggca gcgggggatc ctgatcccct agggccacca tggctcagcg acttcttctg
     1501 aggaggttcc tggcctctgt catctccagg aagccctctc agggtcagtg gccacccctc
     1561 acttccagag ccctccagac cccacaatgc agtcctggtg gcctgactgt aacacccaac
     1621 ccagcccgga caatatacac cacgaggatc tccttgacac ctaggccacc ggttatggtg
     1681 agcaagggcg aggcagtgat caaggagttc atgcggttca aggtgcacat ggagggctcc
     1741 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1801 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttctcctg ggacatcctg
     1861 tcccctcagt tcatgtacgg ctccagggcc ttcaccaagc accccgccga catccccgac
     1921 tactataagc agtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1981 ggcggcgccg tgaccgtgac ccaggacacc tccctggagg acggcaccct gatctacaag
     2041 gtgaagctcc gcggcaccaa cttccctcct gacggccccg taatgcagaa gaagacaatg
     2101 ggctgggaag cgtccaccga gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt
     2161 aagatggccc tgcgcctgaa ggacggaggc cgctacctgg cggacttcaa gaccacctac
     2221 aaggccaaga agcccgtgca gatgcccggc gcctacaacg ttgaccgcaa gttggacatc
     2281 acctcccaca acgaggacta caccgtggtg gaacagtacg aacgctccga gggccgccac
     2341 tccaccggcg gcatggacga gctgtacaag taaactagtt gagatatcaa gcttatcgat
     2401 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2461 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2521 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2581 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2641 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2701 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2761 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2821 gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2881 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     2941 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga
     3001 gcgctgctcg agagatctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3061 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3121 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3181 aggacagcaa gggggaggat tgggaagaca atagcaggca tgcacgtgcg gaccgagcgg
     3241 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     3301 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     3361 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     3421 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     3481 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3541 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3601 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3661 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3721 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3781 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     3841 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3901 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     3961 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4021 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4081 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     4141 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     4201 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4261 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4321 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4381 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4441 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4501 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4561 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4621 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4681 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4741 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4801 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4861 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4921 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4981 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5041 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5101 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5161 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5221 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5281 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5341 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5401 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5461 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5521 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5581 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5641 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5701 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5761 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5821 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5881 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5941 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//