| 质粒名称: | pQCas-8array |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001009 |
| 产品用途: | Integrating cargo gene into bacteria chromosome |
| 存储实验室: | 杨晟Sheng Yang Lab（CEMPS,CAS） |
|
载体骨架基本信息 |
|
| 载体名称: | pCDFDuet-1 |
| 表达系统: | 细菌表达 |
| 启动子: | tT7 |
| 拷贝数: | 中拷贝 |
| 载体抗性: | 大观霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | All |
|
插入基因信息 |
|
| 插入基因名称: | crRNA-array1 |
| 物种来源: | Vibrio cholera |
| 密码子优化: | 未达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multicopy chromosomal integration using CRISPR-associated transposases.
Zhang Y., Sun X., Wang Q., Xu J., Dong F., Yang S., Yang J., Zhang Z., Qian Y., Chen J., Zhang J., Liu Y., Tao R., Jiang Y., Yang J., Yang S.
ACS Synth. Biol. 9, 1998–2008 (2020)
|
买家导航
pQCas-8array载体质粒基本信息
pQCas-8array质粒图谱载体图谱和pQCas-8array载体序列质粒序列多克隆位点信息
pQCas-8array质粒载体简介
pQCas-8array质粒序列载体序列
LOCUS pQCas-8array 8930 bp ds-DNA circular SYN 23-FEB-2020
DEFINITION Bacterial vector with a CloDF13 (CDF) origin for the co-expression
of two genes.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8930)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
JOURNAL Exported Tuesday, Feb 25, 2020 from SnapGene Viewer 5.0.7
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8930
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
primer_bind complement(126..147)
/label=Primer 1
CDS 886..2808
/codon_start=1
/label=Cas8
/translation="MQTLKELIASNPDDLTTELKRAFRPLTPHIAIDGNELDALTILVN
LTDKTDDQKDLLDRAKCKQKLRDEKWWASCINCVNYRQSHNPKFPDIRSEGVIRTQALG
ELPSFLLSSSKIPPYHWSYSHDSKYVNKSAFLTNEFCWDGEISCLGELLKDADHPLWNT
LKKLGCSQKTCKAMAKQLADITLTTINVTLAPNYLTQISLPDSDTSYISLSPVASLSMQ
SHFHQRLQDENRHSAITRFSRTTNMGVTAMTCGGAFRMLKSGAKFSSPPHHRLNSKRSW
LTSEHVQSLKQYQRLNKSLIPENSRIALRRKYKIELQNMVRSWFAMQDHTLDSNILIQH
LNHDLSYLGATKRFAYDPAMTKLFTELLKRELSNSINNGEQHTNGSFLVLPNIRVCGAT
ALSSPVTVGIPSLTAFFGFVHAFERNINRTTSSFRVESFAICVHQLHVEKRGLTAEFVE
KGDGTISAPATRDDWQCDVVFSLILNTNFAQHIDQDTLVTSLPKRLARGSAKIAIDDFK
HINSFSTLETAIESLPIEAGRWLSLYAQSNNNLSDLLAAMTEDHQLMASCVGYHLLEEP
KDKPNSLRGYKHAIAECIIGLINSITFSSETDPNTIFWSLKNYQNYLVVQPRSINDETT
DKSSL"
CDS 2780..3838
/codon_start=1
/label=Cas7
/translation="MKLPTNLAYERSIDPSDVCFFVVWPDDRKTPLTYNSRTLLGQMEA
ASLAYDVSGQPIKSATAEALAQGNPHQVDFCHVPYGASHIECSFSVSFSSELRQPYKCN
SSKVKQTLVQLVELYETKIGWTELATRYLMNICNGKWLWKNTRKAYCWNIVLTPWPWNG
EKVGFEDIRTNYTSRQDFKNNKNWSAIVEMIKTAFSSTDGLAIFEVRATLHLPTNAMVR
PSQVFTEKESGSKSKSKTQNSRVFQSTTIDGERSPILGAFKTGAAIATIDDWYPEATEP
LRVGRFGVHREDVTCYRHPSTGKDFFSILQQAEHYIEVLSANKTPAQETINDMHFLMAN
LIKGGMFQHKGD"
misc_feature 3844..4440
/label=Cas6
primer_bind complement(4456..4474)
/label=T7 Terminator Primer
terminator 4470..4517
/label=T7 terminator
CDS complement(4688..5479)
/codon_start=1
/label=SmR
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
promoter complement(5480..5571)
/label=AmpR promoter
rep_origin complement(5619..6422)
/direction=LEFT
/label=CloDF13 ori
misc_feature 6301..6365
/label=插入序列
primer_bind complement(6559..6577)
/label=QcasR
CDS complement(6632..7714)
/codon_start=1
/label=lacI
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(7715..7792)
/label=lacI promoter
primer_bind 7795..7813
/label=ACYCDuetUP1 Primer
promoter 7838..7856
/label=T7 promoter
protein_bind 7857..7881
/label=lac operator
primer_bind 7875..7899
/label=KpnIcasF
RBS 7912..7917
misc_feature 7923..8442
/label=crRNA-array1
misc_feature 8443..8499
/label=MCS-1
primer_bind 8520..8539
/label=DuetUP2 Primer
primer_bind complement(8520..8539)
/label=DuetDOWN1 Primer
promoter 8545..8563
/label=T7 promoter
protein_bind 8564..8588
/label=lac operator
RBS 8617..8622
CDS join(8631..8930,1..885)
/codon_start=1
/label=TniQ
/translation="MFLQRPKPYSDESLESFFIRVANKNGYGDVHRFLEATKRFLQDID
HNGYQTFPTDITRINPYSAKNSSSARTASFLKLAQLTFNEPPELLGLAINRTNMKYSPS
TSAVVRGAEVFPRSLLRTHSIPCCPLCLRENGYASYLWHFQGYEYCHSHNVPLITTCSC
GKEFDYRVSGLKGICCKCKEPITLTSRENGHEAACTVSNWLAGHESKPLPNLPKSYRWG
LVHWWMGIKDSEFDHFSFVQFFSNWPRSFHSIIEDEVEFNLEHAVVSTSELRLKDLLGR
LFFGSIRLPERNLQHNIILGELLCYLENRLWQDKGLIANLKMNALEATVMLNCSLDQIA
SMVEQRILKPNRKSKPNSPLDVTDYLFHFGDIFCLWLAEFQSDEFNRSFYVSRW"
primer_bind complement(8652..8676)
/label=QCAS300R
ORIGIN
1 tactcgccgt caactagcgc ggttgttaga ggtgcagaag tctttcctcg cagtttacta
61 cggacgcact ccatcccctg ctgtcctttg tgtctgcgag aaaatggcta cgcctcctac
121 ctttggcact ttcaggggta cgaatactgc cacagccata acgtaccttt aattaccact
181 tgtagctgtg gtaaggagtt tgactaccga gtatctgggt taaagggcat ttgctgcaaa
241 tgcaaggagc ctatcacctt aaccagcagg gagaacggtc atgaggcagc gtgtactgtt
301 tcaaactggc ttgctggcca tgaatctaaa cctctgccaa atcttcctaa aagctaccga
361 tggggtttag ttcattggtg gatgggtatt aaagatagcg agttcgatca cttttcgttc
421 gttcaatttt tctcaaactg gccaaggtca ttccactcga taatcgaaga tgaagtagag
481 ttcaaccttg agcatgctgt tgtcagcacg tctgaattac gactaaaaga tcttcttggt
541 cgattgtttt tcggttcaat tcggttacct gagcggaatc ttcaacacaa tatcatcctt
601 ggtgagcttc tctgctattt agaaaatcgt ttatggcaag acaagggatt aatcgccaac
661 ctcaaaatga acgcgttaga ggcgactgta atgttaaatt gtagcctcga tcagattgca
721 tcaatggttg aacaacgcat cttgaagcca aatcgaaaaa gcaagcccaa cagccctctt
781 gatgttaccg attatctatt tcatttcggc gatattttct gtctttggtt agctgagttc
841 caaagcgatg agtttaaccg ttcgttttat gtgtcgaggt ggtaaatgca aactctgaaa
901 gaactaatcg catccaatcc tgacgactta acaactgaac ttaagagagc atttcgtcca
961 ctcacaccgc atattgcaat tgatggtaat gaacttgacg cactgacgat attagtcaat
1021 ttaaccgata agactgatga tcagaaagac ctgctcgatc gagccaaatg caagcaaaaa
1081 cttcgagatg aaaaatggtg ggctagctgc ataaattgcg ttaattacag acaaagccat
1141 aacccaaaat tcccggatat acgttctgaa ggcgtgattc gaacccaagc cctgggtgaa
1201 ttaccgagtt tcctactctc gtcctcaaaa atcccaccat accattggtc atatagccat
1261 gattcaaaat acgtcaacaa aagcgcattc ctcaccaatg agttttgttg ggatggtgag
1321 atctcatgtt tgggtgagct tcttaaagat gcagatcacc cactttggaa tactttgaaa
1381 aagttaggtt gttctcaaaa aacttgcaaa gcaatggcaa aacaactagc tgatattact
1441 ctcacgacta tcaatgtcac actcgcacca aattacctga ctcaaatatc tctccccgat
1501 agtgatacat cctacatttc actctctcct gttgcatcgc tatcgatgca aagccacttt
1561 catcagaggc tccaagatga aaatcggcat agcgcgataa cgcggtttag ccgaactacc
1621 aacatggggg tcactgcgat gacatgtggc ggcgcattta gaatgttaaa gtctggcgct
1681 aagttttcca gcccccctca tcaccgatta aacagtaaac gaagttggtt gacgtcagag
1741 catgttcagt cattaaaaca gtaccagcgc ctcaacaaaa gcctcatacc tgaaaactct
1801 cggattgcac tccgtagaaa atacaaaatc gagcttcaaa atatggtcag atcttggttt
1861 gcaatgcaag accataccct tgattcgaac atacttatcc aacatttgaa tcatgaccta
1921 tcttacttag gagccacaaa acgttttgca tacgatccag cgatgaccaa gctctttact
1981 gagcttttga aacgagagtt atcaaattca atcaataatg gtgagcaaca cactaatgga
2041 tcgtttttag tcctaccgaa tatcagggtt tgtggcgcaa cagctttaag ctccccggta
2101 acggtgggga ttccatcact tacagctttc tttggcttcg ttcacgcatt tgaacggaat
2161 ataaatcgca ccacctcatc gtttcgtgtt gaatcctttg cgatatgcgt ccatcaacta
2221 catgtcgaga agcgaggttt gacagcagag tttgtggaaa aaggcgacgg gactatatcc
2281 gctcccgcga cccgggatga ctggcagtgt gatgtcgtat ttagccttat tttgaacacc
2341 aactttgctc aacatattga ccaagatacg ttagttacat cactaccaaa gcgattggct
2401 cggggttcag caaaaattgc gattgatgac tttaaacata tcaactcatt ctcgacatta
2461 gaaacagcga tcgaatctct gccaatagaa gctggtaggt ggttatcact ttacgcacag
2521 tcaaacaata atctaagtga tctattagca gccatgacag aggaccatca gctcatggca
2581 agctgcgtcg gttaccactt gttagaagag cccaaagata aaccaaactc cctcagaggt
2641 tacaaacacg ctatcgccga gtgcatcatt ggactcatta actcaatcac ctttagctca
2701 gagactgatc ccaacacaat cttttggtcg ctaaagaact atcaaaacta cctagtggta
2761 cagccaagga gtatcaacga tgaaactacc gacaaatcta gcctatgagc gctctatcga
2821 cccatcagat gtctgttttt ttgtcgtctg gcccgatgat agaaaaacac ctttaaccta
2881 caattctcgt actctgctcg ggcaaatgga agcggcatca ttagcctatg atgtctcagg
2941 tcaaccaata aaaagtgcca ccgctgaggc gttagctcaa gggaaccctc atcaagttga
3001 tttctgccac gttccatacg gtgcgagtca tattgaatgc agtttctccg tctcgttttc
3061 ttctgaacta cgtcaaccat ataagtgtaa ctcaagcaaa gttaaacaaa cgctagtgca
3121 attagtcgag ctctacgaaa cgaaaatcgg ctggactgag ctagcaaccc gatatttgat
3181 gaacatttgc aacggtaaat ggctgtggaa aaatacccgt aaagcctatt gctggaacat
3241 tgtacttaca ccttggcctt ggaacgggga aaaggttgga tttgaagata ttcgtactaa
3301 ctacacctca cggcaagact ttaaaaataa taaaaattgg tctgctatag ttgaaatgat
3361 caaaaccgca ttttctagta ctgatgggct ggcgatattt gaagtcaggg ccaccttgca
3421 cttgccaacg aatgctatgg tgcggccaag ccaagttttc acagaaaaag aaagtggcag
3481 taaaagtaaa tctaaaactc aaaacagtcg agtttttcag agtacaacta ttgatggtga
3541 acgatcgcca atactagggg cctttaaaac gggagcagct attgcaacca ttgacgactg
3601 gtatcctgaa gccactgagc cactaagggt cggacggttt ggggttcatc gcgaagatgt
3661 cacttgctac cgtcatccgt ctaccggaaa agattttttc tcgatattac aacaagcaga
3721 gcactatatt gaagtgttga gcgccaacaa aactcccgct caagaaacta tcaacgacat
3781 gcacttttta atggctaacc tgattaaggg tgggatgttc cagcataaag gagactgact
3841 gtgaaatggt attataagac aatcaccttt ctgccagagt tgtgcaacaa cgagtcactg
3901 gctgcaaagt gtctccgcgt tctgcatgga tttaactatc agtatgagac acgaaatatc
3961 ggcgtttcat ttccgctttg gtgtgatgca acggttggaa aaaagatttc atttgtcagc
4021 aagaacaaga tagaactcga cttactactt aaacaacact atttcgtcca aatggaacaa
4081 cttcaatact ttcatatatc caacactgtt ctcgtcccag aagattgtac atacgtttcc
4141 tttagacgct gtcaatctat agataagctc acagcagcag ggctggcaag gaaaatcaga
4201 cgcctggaga aacgtgctct atctagaggc gagcaatttg acccatcatc ttttgctcaa
4261 aaagagcata ctgcaatagc gcactaccac tcacttgggg agtccagcaa acagacgaac
4321 cgcaactttc gactcaatat caggatgctc tcggagcaac cccgtgaggg gaactcgatt
4381 tttagtagct atggcttatc aaattcagaa aactcgtttc agcctgtacc cttaatctga
4441 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
4501 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
4561 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc
4621 ctgaaccgac gaccgggtca tcgtggccgg atcttgcggc ccctcggctt gaacgaattg
4681 ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
4741 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
4801 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
4861 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
4921 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
4981 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
5041 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
5101 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
5161 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
5221 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
5281 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
5341 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
5401 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
5461 cgatcaccgc ttccctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
5521 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa atagctagct
5581 cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt
5641 tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc
5701 gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt
5761 ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac
5821 ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg
5881 ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc
5941 gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg
6001 ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca
6061 cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg
6121 gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt
6181 ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt
6241 taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg
6301 aaggttaagt cagttgggga actgcttaac cttcgatcaa accacctccc caggtggttt
6361 tttcgtttac agggcaaaag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
6421 tcttttctac tgaaccgctc tagatttcag tgcaatttat ctcttcaaat gtagcacctg
6481 aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgcccc gcgcccaccg
6541 gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg
6601 agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
6661 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
6721 gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg
6781 cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat
6841 cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc
6901 ccactaccga gatgtccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc
6961 ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca
7021 tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg
7081 gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga
7141 cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct
7201 ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt
7261 cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat
7321 cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt
7381 gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg
7441 cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg
7501 ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca
7561 cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg
7621 cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat
7681 actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt
7741 ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct
7801 cgacgctctc ccttatgcga ctcctgcatt aggaaattaa tacgactcac tataggggaa
7861 ttgtgagcgg ataacaattc ccctgtagaa ataattttgt ttaactttaa taaggagata
7921 taccatgggt gaactgccga gtaggtagct gataacgcct ggcttggcga tgctcgcctg
7981 atcgacaagt gaactgccga gtaggtagct gataacgaga atattgtggc aaaagcgcat
8041 aaggtgctgt gaactgccga gtaggtagct gataacgtca gagtggcgta tccgatgaat
8101 caccacaggt gaactgccga gtaggtagct gataacgcag aagtgcacgg cgtgtgcggc
8161 ggcgagcagt gaactgccga gtaggtagct gataactcga tccgtgggtg tttatgttct
8221 cagcaggagt gaactgccga gtaggtagct gataacttac ttacggcgtt gcgcattctc
8281 attgcaccgt gaactgccga gtaggtagct gataacgcat tactaagctg gcgcgtgaag
8341 ctgtagaagt gaactgccga gtaggtagct gataacgggg cgtaaaaaga aggggcgctg
8401 attaacgcgt gaactgccga gtaggtagct gataacggat ccgaattcga gctcggcgcg
8461 cctgcaggtc gacaagcttg cggccgcata atgcttaagt cgaacagaaa gtaatcgtat
8521 tgtacacggc cgcataatcg aaattaatac gactcactat aggggaattg tgagcggata
8581 acaattcccc atcttagtat attagttaag tataagaagg agatatacat atgtttttgc
8641 aaagacctaa accttacagc gatgaaagtt tagaaagttt ctttatccga gtggctaaca
8701 aaaatggcta cggtgatgtc catcgcttcc tagaagccac taaacgattc cttcaagaca
8761 ttgaccataa tggctatcaa acctttccga ctgatataac tcggataaac ccatactcag
8821 ctaaaaacag ttccagcgca cgaactgcgt cattcctgaa acttgcacaa ttgacattta
8881 atgaaccgcc agagctactt gggttggcaa ttaacagaac aaacatgaaa
//
