pAAVLINKv1-pCALM1-Cre-3'-SHANK3-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-SHANK3-FLAG               8041 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..4754
                     /codon_start=1
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;SPANK-2"
                     /product="SH3 and multiple ankyrin repeat domains protein3"
                     /label=SHANK3
                     /note="shank postsynaptic density protein; proline 
                     richsynapse associated protein 2; shank3 postsynaptic 
                     density protein"
                     /db_xref="GeneID:85358"
                     /db_xref="HGNC:HGNC:14294"
                     /db_xref="MIM:606230"
                     /protein_id="NP_001358973.1"
                     /translation="VGHKQVVALIRQGGNRLVMKVVSVTRKPEEDGARRRAPPPPKRAP
                     STTLTLRSKSMTAELEELASIRRRKGEKLDEMLAAAAEPTLRPDIADADSRAATVKQRP
                     TSRRITPAEISSLFERQGLPGPEKLPGSLRKGIPRTKSVGEDEKLASLLEGRFPRSTSM
                     QDPVREGRGIPPPPQTAPPPPPAPYYFDSGPPPAFSPPPPPGRAYDTVRSSFKPGLEAR
                     LGAGAAGLYEPGAALGPLPYPERQKRARSMIILQDSAPESGDAPRPPPAATPPERPKRR
                     PRPPGPDSPYANLGAFSASLFAPSKPQRRKSPLVKQLQVEDAQERAALAVGSPGPGGGS
                     FAREPSPTHRGPRPGGLDYGAGDGPGLAFGGPGPAKDRRLEERRRSTVFLSVGAIEGSA
                     PGADLPSLQPSRSIDERLLGTGPTAGRDLLLPSPVSALKPLVSGPSLGPSGSTFIHPLT
                     GKPLDPSSPLALALAARERALASQAPSRSPTPVHSPDADRPGPLFVDVQARDPERGSLA
                     SPAFSPRSPAWIPVPARREAEKVPREERKSPEDKKSMILSVLDTSLQRPAGLIVVHATS
                     NGQEPSRLGGAEEERPGTPELAPAPMQSAAVAEPLPSPRAQPPGGTPADAGPGQGSSEE
                     EPELVFAVNLPPAQLSSSDEETREELARIGLVPPPEEFANGVLLATPLAGPGPSPTTVP
                     SPASGKPSSEPPPAPESAADSGVEEADTRSSSDPHLETTSTISTVSSMSTLSSESGELT
                     DTHTSFADGHTFLLEKPPVPPKPKLKSPLGKGPVTFRDPLLKQSSDSELMAQQHHAASA
                     GLASAAGPARPRYLFQRRSKLWGDPVESRGLPGPEDDKPTVISELSSRLQQLNKDTRSL
                     GEEPVGGLGSLLDPAKKSPIAAARLFSSLGELSSISAQRSPGGPGGGASYSVRPSGRYP
                     VARRAPSPVKPASLERVEGLGAGAGGAGRPFGLTPPTILKSSSLSIPHEPKEVRFVVRS
                     VSARSRSPSPSPLPSPASGPGPGAPGPRRPFQQKPLQLWSKFDVGDWLESIHLGEHRDR
                     FEDHEIEGAHLPALTKDDFVELGVTRVGHRMNIERALRQLDGS"
     misc_feature    2729..2734
                     /label=SDS
     misc_feature    3634..3639
                     /label=SDS
     misc_feature    3751..3756
                     /label=SDS
     misc_feature    4423..4428
                     /label=SDS
     CDS             4761..4826
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4830..5261
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4974..4979
                     /label=SDS
     repeat_region   5304..5444
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    5304..5433
                     /label=AAV2 ITR
     repeat_region   5305..5445
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      5519..5974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6256..6360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6361..7221
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7392..7980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtcggacac aagcaggtgg
     1501 tggctctgat tcgccagggt ggcaaccgcc tcgtcatgaa ggttgtgtct gtgacaagga
     1561 agccagaaga ggacggggct cggcgcagag ccccaccgcc ccccaagagg gcccccagca
     1621 ccacactgac cctgcgctcc aagtccatga cagctgagct cgaggaactt gcctccattc
     1681 ggagaagaaa aggggagaag ctggacgaga tgctggcagc cgccgcagag ccaacgctgc
     1741 ggccagacat cgcagacgca gactccagag ccgccaccgt caaacagagg cccaccagtc
     1801 ggaggatcac acccgccgag attagctcat tgtttgaacg ccagggcctc ccaggcccag
     1861 agaagctgcc gggctccttg cggaagggga ttccacggac caagtctgta ggggaggacg
     1921 agaagctggc gtccctgctg gaagggcgct tcccgcggag cacctcgatg caagacccgg
     1981 tgcgcgaggg tcgcggcatc ccgcccccgc cgcagaccgc gccgcctccc ccgcccgcgc
     2041 cctactactt cgactcgggg ccgcccccgg ccttctcgcc gccgcccccg ccgggccgcg
     2101 cctacgacac ggtgcgctcc agcttcaagc ccggcctgga ggcgcgcctg ggcgcgggcg
     2161 ctgccggcct gtacgagccg ggcgcggccc tcggcccgct gccgtatccc gagcggcaga
     2221 agcgcgcgcg ctccatgatc atcctgcagg actcggcgcc cgagtcgggc gacgcccctc
     2281 gacccccgcc cgcggccacc ccgcccgagc gacccaagcg ccggccgcgg ccgcccggcc
     2341 ccgacagccc ctacgccaac ctgggcgcct tcagcgccag cctcttcgct ccgtccaagc
     2401 cgcagcgccg caagagcccc ctggtgaagc agctgcaggt ggaggacgcg caggagcgcg
     2461 cggccctggc cgtgggcagc cccggtcccg gcggcggcag cttcgcccgc gagccctccc
     2521 cgacccaccg cggtccgcgc ccgggtggcc tcgactacgg cgcgggcgat ggcccggggc
     2581 tcgcgttcgg cggcccgggc ccggccaagg accggcggct ggaggagcgg cgccgctcca
     2641 ctgtgttcct gtccgtgggg gccatcgagg gcagcgcccc cggcgcggat ctgccatccc
     2701 tacagccctc ccgctccatc gacgagcgcc tcctggggac cggccccacc gccggccgcg
     2761 acctgctgct gccctccccg gtgtctgccc tgaagccgtt ggtcagcggc ccgagcctgg
     2821 ggccctcggg ttccaccttc atccacccac tcaccggcaa acccctggac cccagctcac
     2881 ccctggccct tgccctggct gcccgagagc gagctctggc ctcccaggcg ccctcccggt
     2941 cccccacacc cgtgcacagt cccgacgccg accgccccgg acccctgttt gtggatgtac
     3001 aggcccggga cccagagcga gggtccctgg cttccccggc tttctcccca cggagcccag
     3061 cctggattcc tgtgcctgct cgcagggagg cagagaaggt cccccgggag gagcggaagt
     3121 cacccgagga caagaagtcc atgatcctca gcgtcctgga cacatccctg cagcggccag
     3181 ctggcctcat cgttgtgcac gccaccagca acgggcagga gcccagcagg ctgggggggg
     3241 ccgaagagga gcgcccgggc accccggagt tggccccggc ccccatgcag tcagcggctg
     3301 tggcagagcc cctgcccagc ccccgggccc agccccctgg tggcaccccg gcagacgccg
     3361 ggccaggcca gggcagctca gaggaagagc cagagctggt gtttgctgtg aacctgccac
     3421 ctgcccagct gtcgtccagc gatgaggaga ccagggagga gctggcccga attgggttgg
     3481 tgccaccccc tgaagagttt gccaacgggg tcctgctggc caccccactc gctggcccgg
     3541 gcccctcgcc caccacggtg cccagcccgg cctcagggaa gcccagcagt gagccacccc
     3601 ctgcccctga gtctgcagcc gactctgggg tggaggaggc tgacacacgc agctccagcg
     3661 acccccacct ggagaccaca agcaccatct ccacggtgtc cagcatgtcc accttgagct
     3721 cggagagcgg ggaactcact gacacccaca cctccttcgc tgacggacac acttttctac
     3781 tcgagaagcc accagtgcct cccaagccca agctcaagtc cccgctgggg aaggggccgg
     3841 tgaccttcag ggacccgctg ctgaagcagt cctcggacag cgagctcatg gcccagcagc
     3901 accacgccgc ctctgccggg ctggcctctg ccgccgggcc tgcccgccct cgctacctct
     3961 tccagagaag gtccaagcta tggggggacc ccgtggagag ccgggggctc cctgggcctg
     4021 aagacgacaa accaactgtg atcagtgagc tcagctcccg cctgcagcag ctgaacaagg
     4081 acacgcgttc cctgggggag gaaccagttg gtggcctggg cagcctgctg gaccctgcca
     4141 agaagtcgcc catcgcagca gctcggctct tcagcagcct cggtgagctg agctccattt
     4201 cagcgcagcg cagccccggg ggcccgggcg gcggggcctc gtactcggtg aggcccagtg
     4261 gccgctaccc cgtggcgaga cgcgccccga gcccggtgaa gcccgcgtcg ctggagcggg
     4321 tggaggggct gggggcgggc gcggggggcg cagggcggcc cttcggcctc acgcccccca
     4381 ccatcctcaa gtcgtccagc ctctccatcc cgcacgagcc caaggaggtg cgcttcgtgg
     4441 tgcgcagcgt gagcgcgcgc agtcgctccc cctcgccgtc gccgctgccc tcgcccgcgt
     4501 ccggccccgg ccccggcgcc cccggcccac gccgaccctt ccagcagaag ccgctgcagc
     4561 tctggagcaa gttcgacgtg ggcgactggc tggagagcat ccacctaggc gagcaccgcg
     4621 accgcttcga ggaccatgag atagaaggcg cgcacctacc cgcgcttacc aaggacgact
     4681 tcgtggagct gggcgtcacg cgcgtgggcc accgcatgaa catcgagcgc gcgctcaggc
     4741 agctggacgg cagctgtaca gactacaaag accatgacgg tgattataaa gatcatgaca
     4801 tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat
     4861 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4921 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4981 gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5041 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg
     5101 tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg
     5161 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     5221 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga gaccggtgtg
     5281 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     5341 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     5401 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     5461 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     5521 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5581 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5641 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5701 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     5761 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5821 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     5881 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5941 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     6001 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     6061 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     6121 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     6181 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6241 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6301 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6361 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6421 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6481 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6541 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6601 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6661 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6721 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6781 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6841 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6901 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6961 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7021 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7081 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7141 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7201 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7261 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7321 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7381 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7441 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7501 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7561 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7621 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7681 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7741 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7801 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7861 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7921 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7981 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8041 t
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