pAAVLINKv1-pCALM1-Cre-3'-ATP7A-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-ATP7A-FLAG                6642 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3355
                     /codon_start=1
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /product="copper-transporting ATPase 1 isoform 1"
                     /EC_number="7.2.2.8"
                     /label=ATP7A
                     /note="isoform 1 is encoded by transcript variant 1;
                     Menkes disease-associated protein; copper pump 1; 
                     copper-transporting ATPase 1; Cu++-transporting P-type 
                     ATPase; ATPase, Cu++ transporting, alpha polypeptide"
                     /db_xref="CCDS:CCDS35339.1"
                     /db_xref="GeneID:538"
                     /db_xref="HGNC:HGNC:869"
                     /db_xref="MIM:300011"
                     /protein_id="NP_000043.4"
                     /translation="EAMPVAKKPGSTVIAGSINQNGSLLICATHVGADTTLSQIVKLVE
                     EAQTSKAPIQQFADKLSGYFVPFIVFVSIATLLVWIVIGFLNFEIVETYFPGYNRSISR
                     TETIIRFAFQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVK
                     VVVFDKTGTITHGTPVVNQVKVLTESNRISHHKILAIVGTAESNSEHPLGTAITKYCKQ
                     ELDTETLGTCIDFQVVPGCGISCKVTNIEGLLHKNNWNIEDNNIKNASLVQIDASNEQS
                     STSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINNDVNDFMTEHERKGRTAVLV
                     AVDDELCGLIAIADTVKPEAELAIHILKSMGLEVVLMTGDNSKTARSIASQVGITKVFA
                     EVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGTGTDVAIEAADVVLI
                     RNDLLDVVASIDLSRKTVKRIRINFVFALIYNLVGIPIAAGVFMPIGLVLQPWMGSAAM
                     AASSVSVVLSSLFLKLYRKPTYESYELPARSQIGQKSPSEISVHVGIDDTSRNSPKLGL
                     LDRIVNYSRASINSLLSDKRSLNSVVTSEPDKHSLLVGDFREDDDTAL"
     misc_feature    2727..2732
                     /label=SDS
     CDS             3362..3427
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3431..3862
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3575..3580
                     /label=SDS
     repeat_region   3905..4045
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3905..4034
                     /label=AAV2 ITR
     repeat_region   3906..4046
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4120..4575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4857..4961
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4962..5822
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5993..6581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggaggcaa tgcctgtggc
     1501 taagaaacct ggcagcacag tgattgctgg ttccattaac cagaacgggt cactgcttat
     1561 ctgcgcaaca catgttggag cagacacaac cctttctcaa attgtcaaac ttgtggaaga
     1621 ggcacaaaca tcaaaggctc ctatccagca gtttgcagac aaactcagtg gctattttgt
     1681 tccttttatt gtttttgttt ccattgccac cctcttggta tggattgtaa ttggatttct
     1741 gaattttgaa attgtggaaa cctactttcc tggctacaat agaagtatct cccgaacaga
     1801 aacgataata cgatttgctt tccaagcctc tatcacagtt ctgtgtattg catgtccctg
     1861 ttcactggga ctggccactc caactgctgt gatggtgggt acaggagtag gtgctcaaaa
     1921 tggcatacta ataaaaggtg gagagccatt ggagatggct cataaggtaa aggtagtggt
     1981 atttgataag actggaacca ttactcacgg aaccccagtg gtgaatcaag taaaggttct
     2041 aactgaaagt aacagaatat cacaccataa aatcttggcc attgtgggaa ctgctgaaag
     2101 taacagtgaa caccctctag gaacagccat aaccaaatat tgcaaacagg agctggacac
     2161 tgaaaccttg ggtacctgca tagatttcca ggttgtgcca ggctgtggta ttagctgtaa
     2221 agtcaccaat attgaaggct tgctacataa gaataactgg aatatagagg acaataatat
     2281 taaaaatgca tccctggttc aaattgatgc cagtaatgaa cagtcatcaa cttcgtcttc
     2341 catgattatt gatgcccaga tctcaaatgc tcttaatgct cagcagtata aagtcctcat
     2401 tggtaaccgg gagtggatga ttagaaatgg tcttgtcatt aataacgatg taaatgattt
     2461 catgactgaa catgagagaa aaggtcggac tgctgtatta gtagcagttg atgatgagct
     2521 gtgtggcttg atagccattg cagacacagt gaagcctgaa gcagaactgg ctatccatat
     2581 tctgaaatct atgggcttag aagtagttct gatgactgga gacaacagta aaacagctag
     2641 atctattgct tctcaggttg gcattactaa ggtgtttgct gaagttctac cttctcacaa
     2701 ggttgctaaa gtgaagcaac ttcaagagga ggggaaacgg gtagcaatgg tgggagatgg
     2761 aatcaatgac tccccagctc tggcaatggc taatgtggga attgctattg gcacaggcac
     2821 agatgtagcc attgaagcag ctgatgtggt tttgataagg aatgatcttc tggatgtagt
     2881 ggcaagtatt gacttatcaa gaaagacagt caagaggatt cggataaatt ttgtctttgc
     2941 tctaatttat aatctggttg gaattcccat agctgctgga gtttttatgc ccattggttt
     3001 ggttttgcag ccctggatgg gatctgcagc aatggctgct tcatctgttt ctgtagtact
     3061 ttcttctctc ttccttaaac tttacaggaa accaacttac gagagttatg aactgcctgc
     3121 ccggagccag ataggacaga agagtccttc agaaatcagc gttcatgttg gaatagatga
     3181 tacctcaagg aattctccta aactgggttt gctggaccgg attgttaatt atagcagagc
     3241 ctctataaac tcactactgt ctgataaacg ctccctaaac agtgttgtta ccagtgaacc
     3301 tgacaagcac tcactcctgg tgggagactt cagggaagat gatgacactg cattatgtac
     3361 agactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga
     3421 tgacaagtaa ctcgagataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3481 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3541 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt
     3601 cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3661 ttgggcactg acaattccgt ggtgtttatt tgtgaaattt gtgatgctat tgctttattt
     3721 gtaaccatct agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3781 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3841 ggagatgtgg gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg
     3901 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     3961 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     4021 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     4081 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     4141 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
     4201 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4261 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4321 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4381 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4441 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     4501 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4561 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4621 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4681 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4741 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     4801 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     4861 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4921 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4981 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5041 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5101 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5161 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5221 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5281 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5341 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5401 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5461 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5521 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5581 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5641 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5701 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5761 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5821 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5881 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5941 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6001 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6061 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6121 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6181 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6241 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6301 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6361 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6421 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6481 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6541 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6601 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//