pAAVLINK-3'-ZNF292-FLAG-bGHpA

LOCUS       pAAVLINK-3'-ZNF292-FLAG-bGHpA                7566 bp DNA     circular SYN 20-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     protein_bind    145..178
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          179..270
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             271..4506
                     /codon_start=1
                     /label=ZNF292
                     /translation="GGNGENAVFPSQVNVANNFSSTNAQQSAPEKVKKDRGRGPNGKER
                     KPKHNKRAKWPAIIRDGKFICSRCYRAFTNPRSLGGHLSKRSYCKPLDGAEIAQELLQS
                     NGQPSLLASMILSTNAVNLQQPQQSTFNPEACFKDPSFLQLLAENRSPAFLPNTFPRSG
                     VTNFNTSVSQEGSEIIKQALETAGIPSTFEGAEMLSHVSTGCVSDASQVNATVMPNPTV
                     PPLLHTVCHPNTLLTNQNRTSNSKTSSIEECSSLPVFPTNDLLLKTVENGLCSSSFPNS
                     GGPSQNFTSNSSRVSVISGPQNTRSSHLNKKGNSASKRRKKVAPPLIAPNASQNLVTSD
                     LTTMGLIAKSVEIPTTNLHSNVIPTCEPQSLVENLTQKLNNVNNQLFMTDVKENFKTSL
                     ESHTVLAPLTLKTENGDSQMMALNSCTTSINSDLQISEDNVIQNFEKTLEIIKTAMNSQ
                     ILEVKSGSQGAGETSQNAQINYNIQLPSVNTVQNNKLPDSSPFSSFISVMPTKSNIPQS
                     EVSHKEDQIQEILEGLQKLKLENDLSTPASQCVLINTSVTLTPTPVKSTADITVIQPVS
                     EMINIQFNDKVNKPFVCQNQGCNYSAMTKDALFKHYGKIHQYTPEMILEIKKNQLKFAP
                     FKCVVPTCTKTFTRNSNLRAHCQLVHHFTTEEMVKLKIKRPYGRKSQSENVPASRSTQV
                     KKQLAMTEENKKESQPALELRAETQNTHSNVAVIPEKQLVEKKSPDKTESSLQVITVTS
                     EQCNTNALTNTQTKGRKIRRHKKEKEEKKRKKPVSQSLEFPTRYSPYRPYRCVHQGCFA
                     AFTIQQNLILHYQAVHKSDLPAFSAEVEEESEAGKESEETETKQTLKEFRCQVSDCSRI
                     FQAITGLIQHYMKLHEMTPEEIESMTASVDVGKFPCDQLECKSSFTTYLNYVVHLEADH
                     GIGLRASKTEEDGVYKCDCEGCDRIYATRSNLLRHIFNKHNDKHKAHLIRPRRLTPGQE
                     NMSSKANQEKSKSKHRGTKHSRCGKEGIKMPKTKRKKKNNLENKNAKIVQIEENKPYSL
                     KRGKHVYSIKARNDALSECTSRFVTQYPCMIKGCTSVVTSESNIIRHYKCHKLSKAFTS
                     QHRNLLIVFKRCCNSQVKETSEQEGAKNDVKDSDTCVSESNDNSRTTATVSQKEVEKNE
                     KDEMDELTELFITKLINEDSTSVETQANTSSNVSNDFQEDNLCQSERQKASNLKRVNKE
                     KNVSQNKKRKVEKAEPASAAELSSVRKEEETAVAIQTIEEHPASFDWSSFKPMGFEVSF
                     LKFLEESAVKQKKNTDKDHPNTGNKKGSHSNSRKNIDKTAVTSGNHVCPCKESETFVQF
                     ANPSQLQCSDNVKIVLDKNLKDCTELVLKQLQEMKPTVSLKKLEVHSNDPDMSVMKDIS
                     IGKATGRGQY"
     CDS             complement(655..666)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    1775..1780
                     /label=SDS
     misc_feature    1835..1840
                     /label=SDS
     misc_feature    2584..2589
                     /label=SDS
     misc_feature    2603..2608
                     /label=SDS
     CDS             4513..4578
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4582..4806
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4829..4969
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4829..4958
                     /label=AAV2 ITR
     rep_origin      5044..5499
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5781..5885
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5886..6746
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6917..7505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
      181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
      241 ctgacatcca ctttgccttt ctctccacag gggggtaatg gtgaaaatgc agtttttcct
      301 tcacaagtga atgttgcaaa taacttcagt agcaccaatg cccaacagtc tgcacctgaa
      361 aaagttaaaa aagaccgtgg gcggggccca aatgggaagg aaagaaaacc taagcacaac
      421 aaaagggcta aatggcctgc aattatcaga gatgggaaat ttatctgtag caggtgttac
      481 agggctttta ctaatcccag atcactgggt gggcacttat ccaagcgatc ttactgtaaa
      541 ccactggatg gagccgaaat tgctcaagaa cttctacaga gtaatggaca gccttctctt
      601 cttgccagca tgattctctc cacaaatgca gtaaatttgc agcagccaca acaatctacc
      661 ttcaatccag aagcatgttt taaagatcca tcatttctac agcttcttgc tgaaaatcgc
      721 tcgccagcat ttttaccaaa tacatttcct cgatctggtg tgactaactt taataccagt
      781 gtcagtcaag aaggtagtga aattattaaa caggctttgg aaactgctgg cattcccagt
      841 acatttgagg gtgccgaaat gctttctcat gtttcaacag gttgtgtctc tgatgcatca
      901 caagtaaatg caacggtgat gccaaatcca actgtaccac ccctgttgca cactgtatgc
      961 catccaaaca ccttgctgac caaccagaat aggacgtcaa actccaaaac ttcctccatt
     1021 gaggaatgta gcagcttgcc tgtttttcca acgaatgact tactactgaa gactgttgaa
     1081 aatggtttgt gctctagttc atttcctaat tctggtgggc catcacaaaa ttttaccagt
     1141 aacagttctc gtgtttctgt tataagtggt cctcagaaca caagatccag tcatttaaat
     1201 aaaaagggaa acagtgcttc taagagaaga aagaaagttg ctcctccact aattgcacct
     1261 aacgcttccc aaaacttggt aacaagtgac ttaacaacaa tgggactcat agcaaagagt
     1321 gttgaaatcc caactactaa ccttcattca aatgtaattc caacttgtga acctcagagt
     1381 ttggtggaaa atctaacaca gaaattaaat aatgttaaca atcagttatt tatgactgat
     1441 gtaaaagaga atttcaaaac cagtcttgag tcccatacag tgttagcccc tttaacatta
     1501 aaaactgaaa atggtgattc ccaaatgatg gctttgaatt catgcacaac ttcaataaat
     1561 tctgatttgc agatttctga agacaatgtt atacaaaact ttgaaaagac tcttgaaatt
     1621 attaaaactg ctatgaattc tcaaatactt gaggtaaaaa gtggatctca gggtgctggt
     1681 gaaacttcac aaaatgctca aataaattat aacattcagc ttccttcagt aaacactgtg
     1741 caaaataaca aattacccga ttcttctccg ttttcctcct ttataagtgt catgccaaca
     1801 aaaagtaaca ttcctcagtc tgaagtatca cataaggagg atcaaataca ggaaatttta
     1861 gaaggcttac agaaattaaa attagaaaat gacctatcca ctccagcatc ccaatgtgta
     1921 ctgataaata catcagtgac actgactccc acgcctgtta aatcaactgc agatatcaca
     1981 gttattcagc cagtttctga aatgataaac attcaattta atgacaaagt taataaaccc
     2041 tttgtgtgtc aaaaccaagg ctgtaactac agtgctatga caaaggatgc actatttaag
     2101 cactatggta aaattcatca atacactcca gaaatgattc ttgaaattaa gaagaatcaa
     2161 ttgaaatttg ctccctttaa atgtgtagta cctacatgta caaaaacatt tacaagaaat
     2221 tctaacctcc gggcacactg tcagttggtg catcatttta caactgaaga aatggtaaag
     2281 ttaaaaatta aaaggcctta tggaagaaaa tctcagagtg aaaatgtgcc ggcctcacga
     2341 agtacacaag tgaaaaaaca gctagctatg acagaggaaa ataaaaagga atctcagcct
     2401 gctttagaat tgagagcaga gacccaaaat acccacagta atgtagcagt gatcccagaa
     2461 aaacaacttg tagaaaaaaa aagtcctgac aaaacagaaa gttctttaca agtgattaca
     2521 gttacttcag aacaatgtaa tacaaatgca ctcacaaaca cacaaaccaa aggacggaag
     2581 attaggaggc ataaaaaaga aaaggaggag aaaaaacgaa agaagccagt ttcccaatcc
     2641 cttgagtttc caacaagata cagtccttac agaccttatc gatgtgttca ccagggatgc
     2701 tttgctgcct ttacgataca gcaaaacttg attctccatt accaggctgt acacaaatca
     2761 gatctacctg cattttcagc agaggtcgaa gaggaaagtg aagctggtaa agaaagtgaa
     2821 gaaactgaaa ctaaacaaac tttgaaagaa tttcgatgtc aggtaagtga ctgttctcga
     2881 attttccaag caattactgg cctaatacaa cactacatga aacttcatga aatgactcct
     2941 gaagaaattg aaagtatgac tgcttcagtg gatgttggga agtttccatg tgaccagtta
     3001 gagtgtaaat cttcatttac tacatatttg aactatgttg ttcatctaga ggcagaccac
     3061 gggattggac taagggcaag taaaacagaa gaagatggtg tatacaaatg tgattgtgaa
     3121 ggctgtgacc gtatatatgc aacccggtcg aatctcctcc gacacatttt taataagcat
     3181 aatgacaaac ataaggctca tttgattcgt ccaagaagat taacaccagg ccaggaaaat
     3241 atgtcaagca aggcaaacca agaaaaatca aagtctaaac atcgggggac caagcacagc
     3301 agatgtggaa aggaaggaat aaaaatgccc aagaccaaac gaaagaaaaa aaataattta
     3361 gaaaacaaga atgcaaagat tgtgcagatt gaagaaaata agccttattc tctgaaacgt
     3421 gggaagcatg tatattctat aaaggctaga aatgatgccc tgtctgagtg tacaagcaga
     3481 tttgtaaccc agtatccatg tatgataaag ggatgtactt cagttgttac aagtgaaagc
     3541 aatataatta gacattataa gtgccataaa ttatctaagg catttacatc acaacaccga
     3601 aatcttctta ttgtattcaa acggtgttgc aactcacaag taaaggaaac gtctgagcaa
     3661 gaaggtgcta agaatgatgt gaaagattct gacacgtgtg tatcagagag caatgataat
     3721 tcaagaacaa cagctacagt ttcacaaaag gaagttgaaa aaaatgaaaa agatgaaatg
     3781 gatgaactaa cagaattgtt tattacaaaa ttaataaatg aagatagcac aagtgtagag
     3841 acccaagcta atacttcttc aaatgtaagt aatgattttc aggaagataa cctctgccag
     3901 tcagaaagac aaaaagcaag taatttgaag agagttaata aggaaaaaaa tgtctcacaa
     3961 aataaaaaaa ggaaagttga aaaagctgaa ccagcatcag cagctgagtt aagtagcgtg
     4021 cgtaaagaag aagaaactgc tgttgccatt caaaccattg aggagcatcc tgcatctttt
     4081 gactggagct cttttaagcc aatgggattt gaagtatcat ttctgaagtt tcttgaggag
     4141 tctgcagtga agcagaagaa aaatactgac aaagaccatc cgaatactgg aaacaaaaaa
     4201 ggatcccatt caaattcaag aaaaaatatt gataagactg ctgtgactag tggaaatcat
     4261 gtatgtcctt gtaaagaaag cgaaacgttt gtacagtttg ccaatccatc acagcttcag
     4321 tgcagtgata atgtaaaaat tgttttagac aagaatctta aagattgcac tgagcttgtc
     4381 ttaaagcaac ttcaggaaat gaaacctacc gtcagtctga aaaaacttga agtacattca
     4441 aatgatccag atatgtctgt tatgaaagat atcagtatag gtaaagccac aggcagaggt
     4501 cagtactgta cagactacaa agaccatgac ggtgattata aagatcatga catcgactac
     4561 aaggatgacg atgacaagta actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4621 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4681 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4741 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4801 ctatggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     4861 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     4921 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     4981 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     5041 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5101 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5161 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5221 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     5281 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5341 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     5401 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     5461 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     5521 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5581 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5641 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5701 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5881 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5941 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6001 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6061 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6421 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6781 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6841 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7561 acatgt
//