| 质粒名称: | pU6-EMX1-PegRNA-H1-Nicking sgRNA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002211 |
| 产品用途: | Simultaneously express pegRNA and nicking sgRNA targeting the EMX1 gene |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pU6 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | U6, H1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EMX1-pegRNA-nicking-sgRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pU6-EMX1-PegRNA-H1-Nicking sgRNA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pU6-EMX1-PegRNA-H1-Nicking sgRNA载体质粒基本信息
pU6-EMX1-PegRNA-H1-Nicking sgRNA质粒图谱载体图谱和pU6-EMX1-PegRNA-H1-Nicking sgRNA载体序列质粒序列多克隆位点信息
pU6-EMX1-PegRNA-H1-Nicking sgRNA质粒载体简介
pU6-EMX1-PegRNA-H1-Nicking sgRNA质粒序列载体序列
LOCUS pU6-EMX1-PegRNA-H1-Nicking sgRNA 5312 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5312)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5312
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 72..320
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 72..92
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 243..262
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
primer_bind 318..343
/label=OL2774
misc_feature 321..340
/label=EMX1 spacer
misc_RNA 341..416
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature 417..438
/label=RTT
primer_bind complement(431..469)
/label=OL2775
misc_feature 439..451
/label=PBS
primer_bind 462..478
/label=OL2772
misc_feature 464..674
/label=H1 promoter
primer_bind 594..613
/label=H1
/note="Human H1 promoter, forward primer"
primer_bind complement(666..707)
/label=OL2776
primer_bind complement(666..683)
/label=OL2773
misc_feature 684..703
/label=EMX1 nickingRNA
misc_RNA 704..779
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature 831..948
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 997..1507
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1426..1444
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1529..2128
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1529..1548)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2025..2045
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 2275..2396
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2286..2305)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2340..2359
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2644..2661)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2815..3403)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2895..2914)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3574..4434)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4197..4216
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4435..4539)
/gene="bla"
/label=AmpR promoter
rep_origin 4566..5021
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4653..4672)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4863..4884
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5152..5200
/note="synthetic polyadenylation signal"
misc_feature 5214..5305
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5254..5273
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg
61 cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
121 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
181 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
241 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
301 tgtggaaagg acgaaacacc gagtccgagc agaagaagaa gttttagagc tagaaatagc
361 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgttg
421 atgtgatggg agccgttctt cttctgctcg gtttttttga acgctgacgt catcaacccg
481 ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg
541 gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat
601 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc
661 tgtatgagga ccacgaaaca ccgctcccca ttggcctgct tcggttttag agctagaaat
721 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
781 ttttttaaag aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
841 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
901 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
961 agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca
1021 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
1081 cggcgccgac cctgggactc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
1141 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
1201 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
1261 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
1321 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
1381 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
1441 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
1501 tccccagggg gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
1561 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
1621 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
1681 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
1741 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
1801 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
1861 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
1921 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
1981 cgagcgcgcc ggggtgcccg ccttcctgga aacctccgcg ccccgcaacc tccccttcta
2041 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
2101 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
2161 aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt
2221 agatcttagc cactttctag agtcggggcg gccggccgct tcgagcagac atgataagat
2281 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
2341 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
2401 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
2461 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg
2521 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
2581 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
2641 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
2701 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
2761 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
2821 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
2881 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
2941 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
3001 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
3061 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
3121 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
3181 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
3241 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
3301 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
3361 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
3421 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
3481 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
3541 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
3601 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
3661 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc
3721 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
3781 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
3841 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
3901 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
3961 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
4021 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
4081 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
4141 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
4201 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
4261 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
4321 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
4381 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
4441 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
4501 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
4561 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
4621 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
4681 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
4741 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
4801 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
4861 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
4921 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
4981 atttaacgcg aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct
5041 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct
5101 accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata
5161 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
5221 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
5281 agtgcaggtg ccagaacatt tctctatcga ta
//
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