CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Pur

LOCUS       CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-...                9693 bp ds-DNA     circular SYN 12-JUL-2021
DEFINITION  Expression vector for encoding a human codon-optimized 
            dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV 
            promoter and U6-driven crRNAs cloning site..
ACCESSION   .
VERSION     .
KEYWORDS    CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Xu C, Zhou Y, Xiao Q, He B, Geng G, Wang Z, Cao B, Dong X, Bai W, 
            Wang Y, Wang X, Zhou D, Yuan T, Huo X, Lai J, Yang H
  TITLE     Programmable RNA editing with compact CRISPR-Cas13 systems from 
            uncultivated microbes.
  JOURNAL   Nat Methods. 2021 May;18(5):499-506. doi: 
            10.1038/s41592-021-01124-4. Epub 2021 May 3.
  PUBMED    33941935
REFERENCE   2  (bases 1 to 9693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9693
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   25..87
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        396..681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        683..960
                     /label=chicken beta-actin promoter
     intron          961..1188
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1209..1229
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3552..3572
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4800..4934
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4837..4856)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4891..4910
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4941..5181
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     4941..4961
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5112..5131
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        5331..5634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5635..5838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5789..5809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5867..5876
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5873..6580
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6018..6036)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             6590..6646
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6647..7243
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7140..7160
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7250..7474
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7831..7865
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        7896..8000
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8001..8861
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8219..8238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9032..9620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9521..9540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctctcgag ttaaccctag aaagataatc atattgtgac gtacgttaaa
       61 gataatcatg cgtaaaattg acgcatgtgt tttatcgatc tgtatatcga ggtttattta
      121 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      181 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      241 ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac
      301 tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat acataataga
      361 aacaaattat acttattaat taatctagag gtacccgtta cataacttac ggtaaatggc
      421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg
      481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      601 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      661 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      721 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      781 atgggggcgg gggggggggg ggggcgcgcg ccgggggggg gggggggggg sggggggsgg
      841 ggsggggsga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      901 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      961 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
     1021 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     1081 ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta
     1141 ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg
     1201 ccaccatgcc taagaaaaag cgtaaggtcg cccaggtgag caagcagacc tccaagaaga
     1261 gggagctgag catcgacgag taccagggcg cccggaagtg gtgcttcacc attgccttca
     1321 acaaggccct ggtgaaccgg gacaagaacg acggcctgtt cgtggaaagc ctgctgagac
     1381 acgagaagta cagcaagcac gactggtacg acgaagatac ccgggccctg atcaagtgca
     1441 gcacccaggc cgccaacgcc aaggctgaag ccctggcgaa ctacttcagt gcttaccggc
     1501 atagccctgg ctgcctgacc ttcaccgccg aggacgaact gcggaccatc atggagagag
     1561 cctatgagcg ggccatcttc gagtgcagaa gaagagagac agaggtgatc atcgagtttc
     1621 ccagcctgtt cgagggcgac cggatcacca ccgccggcgt ggtgtttttc gtgagctttt
     1681 tcgtggaaag aagagtgctg gatcggctgt atggagccgt gtccggcctg aagaagaatg
     1741 agggacagta caagctgacc cggaaggccc tgagcatgta ctgcctgaag gacagcagat
     1801 tcaccaaggc ctgggataag cgggtgctgc tgttcagaga catcctggcc cagctgggaa
     1861 gaatccccgc cgaggcctac gagtactacc acggcgagca gggtgataag aagagagcta
     1921 acgacaatga gggcacaaat cccaagcggc acaaggacaa gttcatcgaa tttgcactgc
     1981 actacctgga agcccagcac agcgagatct gcttcggcag acgccacatc gtgcgggaag
     2041 aggccggcgc cggcgatgag cacaagaagc accggaccaa gggaaaggtg gtggtggact
     2101 tcagcaagaa ggacgaggac cagagctact atatctccaa gaacaacgtg atcgtgcgga
     2161 tcgacaagaa cgccggccct agaagctacc ggatgggcct gaacgagctg aagtacctcg
     2221 tgctgctgag cctgcagggg aagggcgacg atgccatcgc caagctgtac agatacagac
     2281 agcacgtgga gaacatcctg gatgtggtga aggtgaccga taaggataac cacgtgttcc
     2341 tgccccgctt cgtgctggag cagcacggca tcggcagaaa ggccttcaag cagcggatcg
     2401 atggacgggt gaagcacgtg cggggcgtgt gggagaagaa gaaggccgcc accaatgaaa
     2461 tgaccctgca cgagaaggcc agagacatcc tgcagtacgt gaacgaaaac tgcacccggt
     2521 ccttcaaccc tggcgaatac aacagactgc tggtgtgcct ggtgggcaag gacgtggaga
     2581 actttcaggc cggcctgaag cggctgcagc tggccgaaag gatcgatggc cgggtgtact
     2641 ccatcttcgc ccagaccagc accatcaatg agatgcacca ggtggtgtgc gaccagatcc
     2701 tgaaccggct gtgcagaatc ggcgaccaga agctgtacga ttacgtggga ctgggcaaga
     2761 aggacgaaat cgactacaag cagaaggtgg cctggttcaa ggagcacatc agcatccgga
     2821 gaggattcct gagaaagaag ttctggtacg atagcaagaa gggattcgca aagctggtgg
     2881 aggaacacct ggagtccggc ggcggccagc gcgacgtggg cctggacaag aagtactacc
     2941 acatcgacgc catcggcaga ttcgagggcg ccaaccccgc cctgtacgag accctggcca
     3001 gagatcggct gtgcctcatg atggcccagt acttcctggg cagcgtgaga aaggaactgg
     3061 gcaacaagat tgtgtggagc aacgacagca tcgaactgcc tgtggaaggc tctgtgggaa
     3121 atgagaagag catcgtgttc tccgtgtctg actacggcaa gctgtacgtg ctggacgatg
     3181 ccgaattcct gggccggatc tgcgaatact tcatgcccca cgaaaagggc aagatccggt
     3241 accacacagt gtacgaaaag ggctttagag catacaacga cctgcagaag aagtgcgtgg
     3301 aggccgtgct ggctttcgaa gagaaggtgg tgaaggccaa gaagatgagc gagaaggaag
     3361 gcgcccacta catcgacttc cgggagatcc tggcccagac catgtgcaag gaggccgaaa
     3421 agaccgcagt gaacaaggtg gcggctgcct tcttcgctgc gcacctgaag ttcgtgattg
     3481 acgagttcgg cctgttcagc gacgtgatga agaagtacgg catcgagaag gaatggaagt
     3541 tccctgtcaa gcccaagaag aagcggaagg tgggtggagg cggaggttct gggggaggag
     3601 gtagtggcgg tggtggttca ggaggcggcg gaagccagct gcatttaccg caggttttag
     3661 ctgacgctgt ctcacgcctg gtcataggta agtttggtga cctgaccgac aacttctcct
     3721 cccctcacgc tcgcagaata ggtctggctg gagtcgtcat gacaacaggc acagatgtta
     3781 aagatgccaa ggtgatatgt gtttctacag gagcaaaatg tattaatggt gaatacctaa
     3841 gtgatcgtgg ccttgcatta aatgactgcc atgcagaaat agtatctcgg agatccttgc
     3901 tcagatttct ttatacacaa cttgagcttt acttaaataa cgaggatgat caaaaaagat
     3961 ccatctttca gaaatcagag cgaggggggt ttaggctgaa ggagaatata cagtttcatc
     4021 tgtacatcag cacctctccc tgtggagatg ccagaatctt ctcaccacat gaggcaatcc
     4081 tggaagaacc agcagataga cacccaaatc gtaaagcaag aggacagcta cggaccaaaa
     4141 tagaggctgg tcaggggacg attccagtgc gcaacaatgc gagcatccaa acgtgggacg
     4201 gggtgctgca aggggagcgg ctgctcacca tgtcctgcag tgacaagatt gcacgctgga
     4261 acgtggtggg catccaggga tcactgctca gcattttcgt ggagcccatt tacttctcga
     4321 gcatcatcct gggcagcctt taccacgggg accacctttc cagggccatg taccagcgga
     4381 tctccaacat agaggacctg ccacctctct acaccctcaa caagcctttg ctcacaggca
     4441 tcagcaatgc agaagcacgg cagccaggga aggcccccat attcagtgtc aactggacgg
     4501 taggcgactc cgctattgag gtcatcaacg ccacgactgg gaagggagag ctgggccgcg
     4561 cgtcccgcct gtgtaagcac gcgttgtact gtcgctggat gcgtgtgcac ggcaaggttc
     4621 cctcccactt actacgctcc aagattacca agcccaacgt gtaccatgag acaaagctgg
     4681 cggcaaagga gtaccaggcc gccaaggcgc gtctgttcac agccttcatc aaggcggggc
     4741 tgggggcctg ggtggagaag cccaccgagc aggaccagtt ctcactcacg taaaagctta
     4801 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4861 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4921 atcatgtctg gatcactagt gagggcctat ttcccatgat tccttcatat ttgcatatac
     4981 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
     5041 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     5101 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     5161 tatatatctt gtggaaagga cgaaacaccg gggtcttcga tattcaagcg tcggaagacc
     5221 tgctggagca gcccccgatt tgtggggtga ttacagcttt ttttacgcgt tagttattaa
     5281 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     5341 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     5401 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     5461 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     5521 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     5581 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     5641 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     5701 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     5761 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     5821 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca ccatggtgag
     5881 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     5941 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     6001 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     6061 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     6121 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     6181 cttcgatgac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     6241 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     6301 gaaaaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     6361 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     6421 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     6481 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     6541 gggccgccac tccaccggcg gcatggacga gctgtacaag ggtagtgggg caacaaactt
     6601 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgaccg agtacaagcc
     6661 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     6721 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6781 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6841 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6901 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6961 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     7021 caccgtcgga gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     7081 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     7141 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7201 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacttaagc tgtgccttct
     7261 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7321 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7381 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaaaacaat
     7441 agcaggcatg ctggggatgc ggtgggctct atgggtcgac ggcgcgccct agacgaagca
     7501 gctccagcct acacaatcgc tcaagacgtg taatgctttt attatatatt agtcacgata
     7561 tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa
     7621 tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt
     7681 gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct
     7741 cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
     7801 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
     7861 tagggttaag gatccgcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7921 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7981 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8041 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8101 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8161 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8221 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8281 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8341 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8401 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8461 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8521 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8581 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8641 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8701 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8761 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8821 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8881 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8941 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9001 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9061 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9661 gctggccttt tgctcacatg ttctttcctg cgt
//