pDJ750

LOCUS       pDJ750               9251 bp DNA     circular SYN 10-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Aug 5, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     78..99
                     /label=oYW269
                     /note="LA-NheI-F"
     misc_feature    196..226
                     /label=SleepingBeauty-ITR_Lo
                     /label=nonstandard type:  ITR
     misc_feature    392..423
                     /label=SleepingBeauty-ITR_Li
                     /label=nonstandard type:  ITR
     primer_bind     complement(456..476)
                     /label=oDJ744-8
     primer_bind     457..505
                     /label=oDJ744-1
     primer_bind     complement(460..476)
                     /label=oYW179
                     /note="ITR PCR F"
     enhancer        511..814
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        815..1018
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1015..1039
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1099..1129
                     /label=oDJ585-1
     CDS             1104..1127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(1131..1150)
                     /label=INKA-mGreenLantern-R
     primer_bind     1131..1146
                     /label=oDJ587-5
     primer_bind     complement(1350..1368)
                     /label=PAK4-R
     primer_bind     complement(2181..2221)
                     /label=oDJ749-2
     primer_bind     complement(2181..2216)
                     /label=oDJ746-2
     primer_bind     complement(2181..2215)
                     /label=oDJ656-6
     primer_bind     complement(2181..2202)
                     /label=XbaI-PAK4-R
     primer_bind     complement(2181..2202)
                     /label=HindIII-PAK4-R
     primer_bind     complement(2181..2202)
                     /label=HindIII-PAK4-Ra
     primer_bind     complement(2181..2202)
                     /label=oDJ656-2
     primer_bind     complement(2181..2202)
                     /label=HindIII-PAK4-R`b
     primer_bind     2182..2223
                     /label=oDJ749-3
     primer_bind     complement(2182..2201)
                     /label=oDJ585-2
     primer_bind     complement(2182..2201)
                     /label=oDJ744-2
     primer_bind     complement(2182..2201)
                     /label=oDJ580-2
     primer_bind     2192..2221
                     /label=oDJ656-3
     primer_bind     2200..2218
                     /label=oDJ744-7
     primer_bind     2205..2221
                     /label=oDJ653-3
     primer_bind     complement(2215..2232)
                     /label=BGH/pcDNA3.1R
     primer_bind     complement(2215..2232)
                     /label=pcDNA3.1-R(BGH-R)
     terminator      2218..2445
                     /label=bGH PA
     primer_bind     2218..2237
                     /label=oYW188
                     /note="bGH poly A"
     primer_bind     complement(2225..2242)
                     /label=oYW190
                     /note="BGH poly A R"
     primer_bind     complement(2287..2305)
                     /label=oYW303
                     /note="PolyA tail-bGH pA"
     primer_bind     complement(2287..2305)
                     /label=oYW57
                     /note="bGH seq primer R"
     primer_bind     complement(2290..2317)
                     /label=oYW300
                     /note="PolyA tail-bGH pA"
     primer_bind     complement(2290..2313)
                     /label=oYW302
                     /note="bGH pA R"
     primer_bind     2290..2309
                     /label=W2F0627-P099-D03
                     /note="Sequencing primer"
     primer_bind     2348..2367
                     /label=BGH-polyA-R
     primer_bind     complement(2428..2446)
                     /label=oYW202
                     /note="BGH polyA-R"
     primer_bind     complement(2428..2445)
                     /label=oYW56
                     /note="LA-BamHI-PPT-MluI-bGH pA PCR"
     primer_bind     complement(2428..2445)
                     /label=oYW53
                     /note="LA-BamHI-bGH pA"
     primer_bind     complement(2429..2446)
                     /label=oYW282
                     /note="Arm-PCR pYW134-R"
     primer_bind     complement(2444..2471)
                     /label=oDJ749-4
     primer_bind     complement(2445..2479)
                     /label=oDJ653-4
     primer_bind     complement(2445..2466)
                     /label=oDJ656-4
     primer_bind     complement(2445..2466)
                     /label=oDJ750-2
     primer_bind     2450..2492
                     /label=oDJ750-1
     primer_bind     2470..2492
                     /label=oDJ584-1
     primer_bind     2470..2492
                     /label=oDJ656-1
     misc_feature    2501..2601
                     /label=4x STAT binding
     misc_feature    2501..2515
                     /label=STAT3 binding motif
     misc_feature    2525..2544
                     /label=STAT3 binding motif
     misc_feature    2550..2569
                     /label=STAT3 binding motif
     misc_feature    2553..2561
                     /label=TTCNNNgAA
     misc_feature    2575..2594
                     /label=STAT3 binding motif
     misc_feature    2602..2646
                     /label=P_CMVmin
     promoter        2607..2645
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2690..2708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2711..2730)
                     /label=oDJ584-2
     primer_bind     2711..2730
                     /label=oDJ584-3
     primer_bind     2716..2757
                     /label=tet-Chilada-F
     primer_bind     2737..2758
                     /label=cAMP-mGL-F
     primer_bind     2737..2757
                     /label=XbaI-mGL-F
     regulatory      2737..2746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2743..3450
                     /codon_start=1
                     /label=mChilada
                     /translation="MVSKGEEDNMAIIKEYMRFKVHMEGSVNGHEFEIEGEGEGRPFEG
                     TQTAKLKVTKGGPLPFAWHILPPQFQYGSKAYVKHPADIPDYFKLSFPEGFTWEREMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGNTASTERMYPEDGALK
                     GETKWRLKLKDGGHYEAEVKTTYKAKKPVQLPGAYNVDRKLKITYHNEDYTIVEQYERA
                     EARHSTGGMDELYK"
     primer_bind     2747..2763
                     /label=oYS113
     primer_bind     complement(3435..3476)
                     /label=INKA-mGreenLantern-R
     primer_bind     3450..3472
                     /label=oDJ587-5
     CDS             3463..3576
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             3583..4524
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(3676..3694)
                     /label=PAK4-R
     primer_bind     complement(4507..4548)
                     /label=oDJ749-2
     primer_bind     complement(4507..4543)
                     /label=oDJ746-2
     primer_bind     complement(4507..4542)
                     /label=oDJ656-6
     primer_bind     complement(4507..4537)
                     /label=oDJ656-2
     primer_bind     complement(4507..4531)
                     /label=XbaI-PAK4-R
     primer_bind     complement(4507..4528)
                     /label=HindIII-PAK4-R
     primer_bind     complement(4507..4528)
                     /label=HindIII-PAK4-Ra
     primer_bind     complement(4507..4528)
                     /label=HindIII-PAK4-R`b
     primer_bind     4508..4550
                     /label=oDJ749-3
     primer_bind     complement(4508..4527)
                     /label=oDJ585-2
     primer_bind     complement(4508..4527)
                     /label=oDJ744-2
     primer_bind     complement(4508..4527)
                     /label=oDJ580-2
     primer_bind     4518..4548
                     /label=oDJ656-3
     primer_bind     4527..4548
                     /label=oDJ653-3
     primer_bind     4528..4545
                     /label=oDJ744-7
     primer_bind     complement(4542..4559)
                     /label=BGH/pcDNA3.1R
     primer_bind     complement(4542..4559)
                     /label=pcDNA3.1-R(BGH-R)
     terminator      4545..4772
                     /label=bGH PA
     primer_bind     4545..4564
                     /label=oYW188
                     /note="bGH poly A"
     primer_bind     complement(4552..4569)
                     /label=oYW190
                     /note="BGH poly A R"
     primer_bind     complement(4614..4632)
                     /label=oYW303
                     /note="PolyA tail-bGH pA"
     primer_bind     complement(4614..4632)
                     /label=oYW57
                     /note="bGH seq primer R"
     primer_bind     complement(4617..4644)
                     /label=oYW300
                     /note="PolyA tail-bGH pA"
     primer_bind     complement(4617..4640)
                     /label=oYW302
                     /note="bGH pA R"
     primer_bind     4617..4636
                     /label=W2F0627-P099-D03
                     /note="Sequencing primer"
     primer_bind     4675..4694
                     /label=BGH-polyA-R
     primer_bind     complement(4755..4773)
                     /label=oYW202
                     /note="BGH polyA-R"
     primer_bind     complement(4755..4772)
                     /label=oYW56
                     /note="LA-BamHI-PPT-MluI-bGH pA PCR"
     primer_bind     complement(4755..4772)
                     /label=oYW53
                     /note="LA-BamHI-bGH pA"
     primer_bind     complement(4756..4773)
                     /label=oYW282
                     /note="Arm-PCR pYW134-R"
     primer_bind     complement(4771..4793)
                     /label=oDJ749-4
     primer_bind     complement(4772..4815)
                     /label=oDJ750-2
     primer_bind     complement(4772..4795)
                     /label=oDJ656-4
     primer_bind     complement(4772..4794)
                     /label=oDJ653-4
     primer_bind     4777..4793
                     /label=oDJ750-1
     misc_feature    4808..4894
                     /label=CRE regulatory seq
     misc_feature    4808
                     /label=nfkb motif
     promoter        4901..5047
                     /label=P_TAL
     primer_bind     5034..5077
                     /label=cAMP-mGL-F
     primer_bind     5056..5076
                     /label=tet-Chilada-F
     primer_bind     5056..5076
                     /label=XbaI-mGL-F
     regulatory      5056..5065
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5062..5778
                     /codon_start=1
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     5066..5082
                     /label=oYS113
     primer_bind     complement(5763..5804)
                     /label=INKA-mGreenLantern-R
     primer_bind     5778..5800
                     /label=oDJ587-5
     CDS             5791..5904
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             5911..6852
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(6004..6022)
                     /label=PAK4-R
     primer_bind     complement(6835..6881)
                     /label=oDJ749-2
     primer_bind     complement(6835..6876)
                     /label=oDJ746-2
     primer_bind     complement(6835..6875)
                     /label=oDJ656-6
     primer_bind     complement(6835..6870)
                     /label=oDJ656-2
     primer_bind     complement(6835..6862)
                     /label=XbaI-PAK4-R
     primer_bind     complement(6835..6855)
                     /label=HindIII-PAK4-R
     primer_bind     complement(6835..6855)
                     /label=HindIII-PAK4-Ra
     primer_bind     complement(6835..6855)
                     /label=HindIII-PAK4-R`b
     primer_bind     6836..6883
                     /label=oDJ749-3
     primer_bind     complement(6836..6856)
                     /label=oDJ744-2
     primer_bind     complement(6836..6855)
                     /label=oDJ585-2
     primer_bind     complement(6836..6855)
                     /label=oDJ580-2
     primer_bind     6846..6881
                     /label=oDJ656-3
     primer_bind     6856..6878
                     /label=oDJ744-7
     primer_bind     6861..6881
                     /label=oDJ653-3
     primer_bind     complement(6875..6892)
                     /label=BGH/pcDNA3.1R
     primer_bind     complement(6875..6892)
                     /label=pcDNA3.1-R(BGH-R)
     primer_bind     6878..6897
                     /label=oYW188
                     /note="bGH poly A"
     polyA_signal    6881..7105
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6885..6902)
                     /label=oYW190
                     /note="BGH poly A R"
     primer_bind     complement(6947..6965)
                     /label=oYW303
                     /note="PolyA tail-bGH pA"
     primer_bind     complement(6947..6965)
                     /label=oYW57
                     /note="bGH seq primer R"
     primer_bind     complement(6950..6977)
                     /label=oYW300
                     /note="PolyA tail-bGH pA"
     primer_bind     complement(6950..6973)
                     /label=oYW302
                     /note="bGH pA R"
     primer_bind     6950..6969
                     /label=W2F0627-P099-D03
                     /note="Sequencing primer"
     primer_bind     7008..7027
                     /label=BGH-polyA-R
     primer_bind     complement(7088..7106)
                     /label=oYW202
                     /note="BGH polyA-R"
     primer_bind     complement(7088..7105)
                     /label=oYW56
                     /note="LA-BamHI-PPT-MluI-bGH pA PCR"
     primer_bind     complement(7088..7105)
                     /label=oYW53
                     /note="LA-BamHI-bGH pA"
     primer_bind     complement(7089..7106)
                     /label=oYW282
                     /note="Arm-PCR pYW134-R"
     primer_bind     complement(7104..7126)
                     /label=oDJ749-4
     primer_bind     complement(7105..7127)
                     /label=oDJ653-4
     primer_bind     complement(7105..7127)
                     /label=oDJ656-4
     primer_bind     complement(7105..7127)
                     /label=oDJ750-2
     primer_bind     7110..7126
                     /label=oDJ750-1
     primer_bind     7117..7138
                     /label=oYW197
                     /note="ITR PCR F1"
     misc_feature    complement(7189..7217)
                     /label=SleepingBeauty-ITR_Ri
                     /label=nonstandard type:  ITR
     misc_feature    7383..7414
                     /label=SleepingBeauty-ITR-Ro
                     /label=nonstandard type:  ITR
     primer_bind     complement(7557..7576)
                     /label=oYW207
                     /note="pLL3.7 PCR R"
     primer_bind     complement(7558..7583)
                     /label=oYW301
                     /note="PolyA tail-pLL3.7 3'LTR"
     rep_origin      complement(7602..8190)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7741..7760)
                     /label=W5CF0703-P099-D09
                     /note="Sequencing primer"
     CDS             complement(8361..9221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8378..8397)
                     /label=W4CF0701-P099-E08
                     /note="Sequencing primer"
     primer_bind     8556..8567
                     /label=oDJ587-3
     primer_bind     complement(9042..9061)
                     /label=W3CF0630-P099-D08
                     /note="Sequencing primer"
     primer_bind     complement(9098..9117)
                     /label=W3CF0630-P099-A08
                     /note="Sequencing primer"
     primer_bind     complement(9098..9117)
                     /label=W3CF0630-P099-F07
                     /note="Sequencing primer"
     primer_bind     9192..9211
                     /label=oYW24
                     /note="Forward seq primer of AmpR"
     promoter        complement(join(9222..9251,1..75))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
       61 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc
      121 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
      181 attggatccc tatacagttg aagtcggaag tttacataca cttaagttgg agtcattaaa
      241 actcgttttt caactactcc acaaatttct tgttaacaaa caatagtttt ggcaagtcag
      301 ttaggacatc tactttgtgc atgacacaag tcatttttcc aacaattgtt tacagacaga
      361 ttatttcact tataattcac tgtatcacaa ttccagtggg tcagaagttt acatacacta
      421 agttcgactc ctctgcagaa tgcggcgatg tttcggtaag gggtccgcta gaattcgatc
      481 cagtttggtt aattaagctt gggagttccg cgttacataa cttacggtaa atggcccgcc
      541 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      601 aacgccaata gggactttcc attgacgtca atgggtggag catttacggt aaactgccca
      661 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      721 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      781 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      841 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      901 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      961 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     1021 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     1081 acaccgactc tagaggatcc atggactaca aggacgacga tgataagtcc ggatccgaag
     1141 cagaggactg gacagcagcc ctactgaaca ggggtcgcag tcgccagccc ctggtactag
     1201 gggacaattg ctttgctgac ttggtgcaca actggatgga gctgcctgag gaattccccg
     1261 ccgcccctgc tgttcctggg ccccctggcc cccgctcacc acagcgggag ccacagcgag
     1321 tatcccatga gcagttccgg gctgccctgc agctggtggt ggacccaggc gacccccgct
     1381 cctacctgga caacttcatc aagattggcg agggctccac gggcatcgtg tgcatcgcca
     1441 ccgtgcgcag ctcgggcaag ctggtggccg tcaagaagat ggacctgcgc aagcagcaga
     1501 ggcgcgagct gctcttcaac gaggtggtaa tcatgaggga ctaccagcac gagaatgtgg
     1561 tggagatgta caacagctac ctggtggggg acgagctctg ggtggtcatg gagttcctgg
     1621 aaggaggcgc cctcaccgac atcgtcaccc acaccaggat gaacgaggag cagatcgcag
     1681 ccgtgtgcct tgcagtgctg caggccctgt cggtgctcca cgcccagggc gtcatccacc
     1741 gggacatcaa gagcgactcg atcctgctga cccatgatgg cagggtgaag ctgtcagact
     1801 ttgggttctg cgcccaggtg agcaaggaag tgccccgaag gaagtcgctg gtcggcacgc
     1861 cctactggat ggccccagag ctcatctccc gccttcccta cgggccagag gtagacatct
     1921 ggtcgctggg gataatggtg attgagatgg tggacggaga gcccccctac ttcaacgagc
     1981 cacccctcaa agccatgaag atgattcggg acaacctgcc accccgactg aagaacctgc
     2041 acaaggtgtc gccatccctg aagggcttcc tggaccgcct gctggtgcga gaccctgccc
     2101 agcgggccac ggcagccgag ctgctgaagc acccattcct ggccaaggca gggccgcctg
     2161 ccagcatcgt gcccctcatg cgccagaacc gcaccagatg aacccgctga tcagcctcga
     2221 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2281 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     2341 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     2401 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     2461 gaaccatcga tgctctagga agatctccac gcgtctcgag atttcccgta aatcgtcgag
     2521 tggacatttc ccgtaaatcg tcgagtggac atttcccgta aatcgtcgag tggacatttc
     2581 ccgtaaatcg tcgagctgca gtcgagggta ggcgtgtacg gtgggaggtc tatataagca
     2641 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     2701 actataggga gacccaagct gttcgaagcg gaattcgcca ccatggtgag caagggcgag
     2761 gaggacaaca tggctatcat caaggaatac atgcgtttca aggttcatat ggaaggttct
     2821 gttaacggtc atgaattcga aatcgaaggt gaaggtgaag gtcgtccttt cgaaggtacc
     2881 cagaccgcta agctgaaggt taccaagggt ggtcctctgc ctttcgcttg gcatatcctg
     2941 cctcctcagt tccagtacgg ttctaaggct tacgttaagc atcctgctga catccctgac
     3001 tacttcaagc tgtctttccc tgaaggtttc acctgggaac gtgaaatgaa cttcgaagac
     3061 ggtggtgttg ttaccgttac ccaggactct tctctgcagg acggtgaatt catctacaag
     3121 gttaagctgc gtggtaccaa cttcccttct gacggtcctg ttatgcagaa gaagaccatg
     3181 ggtaacaccg cttctaccga acgtatgtac cctgaagacg gtgctctgaa gggtgaaacc
     3241 aagtggcgtc tgaagctgaa ggacggtggt cattacgaag ctgaagttaa gaccacctac
     3301 aaggctaaga agcctgttca gctgcctggt gcttacaacg ttgaccgtaa gctgaagatc
     3361 acctaccata acgaagacta caccatcgtt gaacagtacg aacgtgctga agctcgtcat
     3421 tctaccggtg gcatggacga gctgtacaag tcaggtggat ccgaagcaga ggactggaca
     3481 gcagccctac tgaacagggg tcgcagtcgc cagcccctgg tactagggga caattgcttt
     3541 gctgacttgg tgcacaactg gatggagctg cctgaggaat tccccgccgc ccctgctgtt
     3601 cctgggcccc ctggcccccg ctcaccacag cgggagccac agcgagtatc ccatgagcag
     3661 ttccgggctg ccctgcagct ggtggtggac ccaggcgacc cccgctccta cctggacaac
     3721 ttcatcaaga ttggcgaggg ctccacgggc atcgtgtgca tcgccaccgt gcgcagctcg
     3781 ggcaagctgg tggccgtcaa gaagatggac ctgcgcaagc agcagaggcg cgagctgctc
     3841 ttcaacgagg tggtaatcat gagggactac cagcacgaga atgtggtgga gatgtacaac
     3901 agctacctgg tgggggacga gctctgggtg gtcatggagt tcctggaagg aggcgccctc
     3961 accgacatcg tcacccacac caggatgaac gaggagcaga tcgcagccgt gtgccttgca
     4021 gtgctgcagg ccctgtcggt gctccacgcc cagggcgtca tccaccggga catcaagagc
     4081 gactcgatcc tgctgaccca tgatggcagg gtgaagctgt cagactttgg gttctgcgcc
     4141 caggtgagca aggaagtgcc ccgaaggaag tcgctggtcg gcacgcccta ctggatggcc
     4201 ccagagctca tctcccgcct tccctacggg ccagaggtag acatctggtc gctggggata
     4261 atggtgattg agatggtgga cggagagccc ccctacttca acgagccacc cctcaaagcc
     4321 atgaagatga ttcgggacaa cctgccaccc cgactgaaga acctgcacaa ggtgtcgcca
     4381 tccctgaagg gcttcctgga ccgcctgctg gtgcgagacc ctgcccagcg ggccacggca
     4441 gccgagctgc tgaagcaccc attcctggcc aaggcagggc cgcctgccag catcgtgccc
     4501 ctcatgcgcc agaaccgcac cagatgaagc tcgctgatca gcctcgactg tgccttctag
     4561 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4621 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4681 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4741 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccaggacgcg
     4801 tgctagcgca ccagacagtg acgtcagctg ccagatccca tggccgtcat actgtgacgt
     4861 ctttcagaca ccccattgac gtcaatggga gaacagatct gccgccccga ctgcatctgc
     4921 gtgttcgaat tcgccaatga caagacgctg ggcggggttt gtgtcatcat agaactaaag
     4981 acatgcaaat atatttcttc cggggacacc gccagcaaac gcgagcaacg ggccacgggg
     5041 atgaagcaga agcttgccac catggtgagc aagggcgagg agctgttcac cggcgtggtg
     5101 cctatcctgg tggagctgga cggcgacgtg aacggccaca agttcagcgt gaggggcgag
     5161 ggcgagggcg acgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag
     5221 ctgcctgtgc cttggcctac cctggtgacc accctgggct acggcgtggc ctgcttcgcc
     5281 aggtaccctg accacatgaa gcagcacgac ttcttcaaga gcgccatgcc tgagggctac
     5341 gtgcaggaga ggaccatcag cttcaaggac gacggcacct acaagaccag ggccgaggtg
     5401 aagttcgagg gcgacaccct ggtgaacagg atcgtgctga agggcatcga cttcaaggag
     5461 gacggcaaca tcctgggcca caagctggag tacaacttca acagccacaa ggtgtacatc
     5521 accgccgaca agcagaagaa cggcatcaag gccaacttca agaccaggca caacgtggag
     5581 gacggcggcg tgcagctggc cgaccactac cagcagaaca cccctatcgg cgacggccct
     5641 gtgctgctgc ctgacaacca ctacctgagc caccagagca agctgagcaa ggaccctaac
     5701 gagaagaggg accacatggt gctgaaggag agggtgaccg ccgccggcat cacccacgac
     5761 atggacgagc tgtacaagtc aggtggatcc gaagcagagg actggacagc agccctactg
     5821 aacaggggtc gcagtcgcca gcccctggta ctaggggaca attgctttgc tgacttggtg
     5881 cacaactgga tggagctgcc tgaggaattc cccgccgccc ctgctgttcc tgggccccct
     5941 ggcccccgct caccacagcg ggagccacag cgagtatccc atgagcagtt ccgggctgcc
     6001 ctgcagctgg tggtggaccc aggcgacccc cgctcctacc tggacaactt catcaagatt
     6061 ggcgagggct ccacgggcat cgtgtgcatc gccaccgtgc gcagctcggg caagctggtg
     6121 gccgtcaaga agatggacct gcgcaagcag cagaggcgcg agctgctctt caacgaggtg
     6181 gtaatcatga gggactacca gcacgagaat gtggtggaga tgtacaacag ctacctggtg
     6241 ggggacgagc tctgggtggt catggagttc ctggaaggag gcgccctcac cgacatcgtc
     6301 acccacacca ggatgaacga ggagcagatc gcagccgtgt gccttgcagt gctgcaggcc
     6361 ctgtcggtgc tccacgccca gggcgtcatc caccgggaca tcaagagcga ctcgatcctg
     6421 ctgacccatg atggcagggt gaagctgtca gactttgggt tctgcgccca ggtgagcaag
     6481 gaagtgcccc gaaggaagtc gctggtcggc acgccctact ggatggcccc agagctcatc
     6541 tcccgccttc cctacgggcc agaggtagac atctggtcgc tggggataat ggtgattgag
     6601 atggtggacg gagagccccc ctacttcaac gagccacccc tcaaagccat gaagatgatt
     6661 cgggacaacc tgccaccccg actgaagaac ctgcacaagg tgtcgccatc cctgaagggc
     6721 ttcctggacc gcctgctggt gcgagaccct gcccagcggg ccacggcagc cgagctgctg
     6781 aagcacccat tcctggccaa ggcagggccg cctgccagca tcgtgcccct catgcgccag
     6841 aaccgcacca gatgatctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     6901 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6961 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     7021 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     7081 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg ggcctactag
     7141 ctactcggga ccccttaccg aaacatcgcc gcattctgca gaggagtcga gtgtatgtaa
     7201 acttctgacc cactgggaat gtgatgaaag aaataaaagc tgaaatgaat cattctctct
     7261 actattattc tgatatttca cattcttaaa ataaagtggt gatcctaact gacctaagac
     7321 agggaatttt tactaggatt aaatgtcagg aattgtgaaa aagtgagttt aaatgtattt
     7381 ggctaaggtg tatgtaaact tccgacttca actgtatagg gatccgcttc ctcgctcact
     7441 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7501 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7561 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7621 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7681 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7741 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7801 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7861 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7921 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7981 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8041 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8101 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8161 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8221 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8281 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8341 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8401 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8461 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8521 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8581 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8641 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8701 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8761 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8821 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8881 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8941 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9001 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9061 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9121 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9181 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9241 tattgaagca t
//