| 质粒名称: | pAAV-hSyn-GRAB_DA2h |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000114 |
| 产品用途: | Expresses the DA sensor GRAB_DA2h in neurons |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | GRAB_DA2h |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Next-generation GRAB sensors for monitoring dopaminergic activity in vivo
Sun F., Zhou J., Dai B., Qian T., Zeng J., Li X., Zhuo Y., Zhang Y., Wang Y., Qian C., Tan K., Feng J., Dong H., Lin D., Cui G., Li Y.
Nat. Methods 2020 1711 17, 1156–1166 (2020)
|
买家导航
pAAV-hSyn-GRAB_DA2h载体质粒基本信息
pAAV-hSyn-GRAB_DA2h质粒图谱载体图谱和pAAV-hSyn-GRAB_DA2h载体序列质粒序列多克隆位点信息
pAAV-hSyn-GRAB_DA2h质粒载体简介
pAAV-hSyn-GRAB_DA2h质粒序列载体序列
LOCUS pAAV-hSyn-GRAB_DA2h 6341 bp DNA circular SYN 21-APR-2023
DEFINITION Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in
neurons.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6341)
AUTHORS Sun F, Zhou J, Dai B, Qian T, Zeng J, Li X, Zhuo Y, Zhang Y, Wang Y,
Qian C, Tan K, Feng J, Dong H, Lin D, Cui G, Li Y.
TITLE Next-generation GRAB sensors for monitoring dopaminergic activity in
vivo.
JOURNAL Nature Methods (2020)
REFERENCE 2 (bases 1 to 6341)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nature
Methods (2020)"
FEATURES Location/Qualifiers
source 1..6341
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 131..574
/label=hSyn
regulatory 581..590
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
sig_peptide 587..649
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 650..674
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 674..2443
/codon_start=1
/label=GRAB_DA2h
/translation="TTGDPLNLSWYDDDLERQNWSRPFNGSDGKADRPHYNYYATLLTL
LIAVIVFGNVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVYLEVVGEWKF
SRIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISIVWV
LSFTISCPLLFGLNNADQNECIIANPAFVVYSSIVSFYVPFIVMLLVYIKIYIVLRRRR
KRVNTKRSSRAFRAHLRAPLKGNCTHPEDMKLGGTVYIKADKQKNGIKANFHIRHNIED
GGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLG
MDELYKGGTGGKSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAAMSRRKLSQQKEKKA
TQMLAIVLGVFIICWLPFFITHILNIHCDCNIPPVLYSAFTWLGYVNSAVNPIIYTTFN
IEFRKAFLKILHC"
primer_bind 1213..1234
/label=WKG114-F
primer_bind 1648..1669
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1778..1799)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2039..2058)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 2468..3056
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2521..2541)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2939..2950)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3088..3564
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3287..3306)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3604..3744
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3604..3733
/label=AAV2 ITR
rep_origin 3819..4274
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3906..3925)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4116..4137
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4291..4310)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4410..4432
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4470..4488)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4556..4660
/gene="bla"
/label=AmpR promoter
CDS 4661..5521
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4879..4898)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5692..6280
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6181..6200
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtatgagg cggggtgggg gtgcctacct gacgaccgac
181 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
241 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
301 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
361 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
421 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
481 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
541 ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggt gccaccatgg agacagacac
601 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgaca caagtttgta
661 caaaaaagtt ggcaccaccg gtgatccact gaatctgtcc tggtatgatg atgatctgga
721 gaggcagaac tggagccggc ccttcaacgg gtcagacggg aaggcggaca gaccccacta
781 caactactat gccacactgc tcaccctgct catcgctgtc atcgtcttcg gcaacgtgct
841 ggtgtgcatg gctgtgtccc gcgagaaggc gctgcagacc accaccaact acctgatcgt
901 cagcctcgca gtggccgacc tcctcgtcgc cacactggtc atgccctggg ttgtctacct
961 ggaggtggta ggtgagtgga aattcagcag gattcactgt gacatcttcg tcactctgga
1021 cgtcatgatg tgcacggcga gcatcctgaa cttgtgtgcc atcagcatcg acaggtacac
1081 agctgtggcc atgcccatgc tgtacaatac gcgctacagc tccaagcgcc gggtcaccgt
1141 catgatctcc atcgtctggg tcctgtcctt caccatctcc tgcccactcc tcttcggact
1201 caataacgca gaccagaacg agtgcatcat tgccaacccg gccttcgtgg tctactcctc
1261 catcgtctcc ttctacgtgc ccttcattgt catgctgctg gtctacatca agatctacat
1321 tgtcctccgc agacgccgca agcgagtcaa caccaaacgc agcagccgag ctttcagggc
1381 ccacctgagg gctccactaa agggcaactg tactcacccc gaggacatga aactcggagg
1441 aactgtctat atcaaggccg acaagcagaa gaacggcatc aaggcgaact tccacatccg
1501 ccacaacatc gaggacggcg gcgtgcagct cgcctaccac taccagcaga acacccccat
1561 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccaaactttc
1621 gaaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
1681 gatcactctc ggcatggacg agctgtacaa gggcggtacc ggagggaaat ccatggtgag
1741 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
1801 aaacggccac aagttcagcg tgtccggcga gggtgagggc gatgccacct acggcaagct
1861 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
1921 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
1981 cttcttcaag tccgccatgc ccgaaggcta catccaggag cgcaccatct tcttcaagga
2041 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
2101 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
2161 gtacaacact ggagcagcag caatgagccg taggaagctc tcccagcaga aggagaagaa
2221 agccactcag atgctcgcca ttgttctcgg cgtgttcatc atctgctggc tgcccttctt
2281 catcacacac atcctgaaca tacactgtga ctgcaacatc ccgcctgtcc tgtacagcgc
2341 cttcacgtgg ctgggctatg tcaacagcgc cgtgaacccc atcatctaca ccaccttcaa
2401 cattgagttc cgcaaggcct tcctgaagat cctccactgc tgagaattcg atatcaagct
2461 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
2521 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
2581 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
2641 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
2701 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
2761 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
2821 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
2881 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
2941 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
3001 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
3061 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
3121 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
3181 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
3241 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
3301 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
3361 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
3421 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
3481 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
3541 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
3601 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
3661 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
3721 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
3781 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
3841 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
3901 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
3961 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
4021 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
4081 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
4141 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
4201 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
4261 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
4321 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
4381 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
4441 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
4501 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
4561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
4621 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
4681 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
4741 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
4801 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
4861 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
4921 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
4981 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
5041 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
5101 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
5161 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
5221 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
5281 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
5341 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
5401 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
5461 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
5521 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
5581 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
5641 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
5701 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
5761 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
5821 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
5881 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
5941 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
6001 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
6061 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
6121 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
6181 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
6241 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
6301 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
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