| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RERE-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001376 |
| 产品用途: | 3' AAVLINK plasmid for RERE expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RERE |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RERE-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RERE-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RERE-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RERE-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RERE-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RERE-FLAG 6280 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6280)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6280
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 1545..1550
/label=SDS
CDS 1547..3015
/codon_start=1
/label=RERE
/translation="EAA*RGGRPAHSPPSRSRRRLWTTLRSLRAPLPHQGARPRSPLWW
TPPVTPASQLGSTNTWTGATTRVPGQTCTSCLWPGPSWPRRGRRPLRRPSARLSRKPER
SESGRRRRRRSGSGSESGSARQSGRLRRPAQRMKVASVTHSSVVLATCGHPSSHHQPPL
LLCPPTSGPTHLPFGL*ASTPGPTSCRPPTATTPSTCPLTPRTPCWPTTCLASTTSTPP
SASGSSGSGRSESGRSESGSCGRG*SRASR*SPQSWTPCTQPPTPWSTLPGTAPSPSPR
PPGPTLLLLSTRA*TPWRGRDWPWRAPSCGPR*ATLTDWQPSVSTQSAWHR*PAIPWPD
CRCST*LRTITSTLTFTPTSTSTSRTPSTKVQQAPFTRWSTP*LPVPTWLASPTRLALS
PTLCLDSPHTSTRCFATQFSAPPTPVTCLGPSHPPCQQPTSCRPCMPSRPSCRDWPWSS
SGCMDTPTCMVATYQVRKIITVD*RKKVTSS"
misc_feature 1598..1603
/label=SDS
misc_feature 1601..1606
/label=SDS
misc_feature 1808..1813
/label=SDS
CDS 3022..3087
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3091..3522
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3235..3240
/label=SDS
repeat_region 3565..3683
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3565..3672
/label=AAV2 ITR
rep_origin 3758..4213
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4495..4599
/gene="bla"
/label=AmpR promoter
CDS 4600..5460
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5631..6219
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggagg cagcatagcg
1561 ggggggtcgt cctgcccact ccccaccgtc cagatcaagg aggaggctct ggacgacgct
1621 gaggagcctg agagcccccc tcccccacca aggagcccgt ccccggagcc cactgtggtg
1681 gacaccccca gtcacgccag ccagtcagct aggttctaca aacacctgga ccggggctac
1741 aactcgtgtg cccggacaga cctgtacttc atgcctctgg ccgggtccaa gctggccaag
1801 aagagggagg aggccattga gaaggccaag cgcgaggctg agcagaaagc ccgagaggag
1861 cgagagcggg agaaggagaa ggagaaggag cgggagcggg agcgagagcg ggagcgcgag
1921 gcagagcggg cggctaaggc gtccagctca gcgcatgaag gtcgcctcag tgacccacag
1981 ctcagtggtc ctggccacat gcggccatcc ttcgagccac caccaaccac cattgctgct
2041 gtgcccccct acatcgggcc cgacacacct gcccttcgga ctctgagcga gtacgcccgg
2101 ccccacgtca tgtcgcccac caaccgcaac caccccttct acatgcccct taaccccacg
2161 gaccccctgc tggcctacca catgcctggc ctctacaacg tcgaccccac catccgcgag
2221 cgggagctcc gggagcggga gatccgagag cgggagatcc gagagcggga gctgcgggag
2281 aggatgaagc cgggcttcga ggtgaagccc ccagagctgg accccctgca cccagccgcc
2341 aaccccatgg agcactttgc ccggcacagc gccctcacca tccccccgac cgccgggccc
2401 cacccttttg cttctttcca cccgggcctg aaccccttgg agagggagag actggccctg
2461 gcgggccccc agctgcggcc cgagatgagc taccctgaca gactggcagc cgagcgtatc
2521 cacgcagagc gcatggcatc gctgaccagc gatcccctgg cccgactgca gatgttcaac
2581 gtgactccgc accatcacca gcactctcac attcactccc acctccacct ccaccagcag
2641 gaccccctcc accaaggttc agcaggcccc gttcacccgc tggtcgaccc cctgactgcc
2701 ggtccccacc tggctcgctt cccctacccg cctggcactc tccccaaccc tctgcttgga
2761 cagcccccac acgagcacga gatgcttcgc cacccagttt tcggcacccc ctacccccgt
2821 gacctgcctg gggccatccc accccccatg tcagcagccc accagctgca ggccatgcat
2881 gcccagtcgg ccgagctgca gagactggcc atggagcagc agtggctgca tggacacccc
2941 cacatgcatg gtggccacct accaagtcag gaagattatt acagtcgact gaagaaagaa
3001 ggtgacaagc agttatgtac agactacaaa gaccatgacg gtgattataa agatcatgac
3061 atcgactaca aggatgacga tgacaagtaa ctcgagataa tcaacctctg gattacaaaa
3121 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
3181 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
3241 tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct
3301 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgtttatt tgtgaaattt
3361 gtgatgctat tgctttattt gtaaccatct agctttattt gtgaaatttg tgatgctatt
3421 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
3481 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagatctgg agaccggtgt
3541 ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
3601 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccggcct cagtgagcga
3661 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
3721 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
3781 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
3841 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
3901 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
3961 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
4021 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
4081 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
4141 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
4201 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
4261 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
4321 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
4381 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
4441 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
4501 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
4561 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
4621 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
4681 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
4741 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
4801 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
4861 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
4921 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
4981 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
5041 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
5101 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
5161 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
5221 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
5281 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
5341 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
5401 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
5461 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
5521 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
5581 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
5641 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
5701 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
5761 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
5821 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
5881 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
5941 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
6001 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
6061 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
6121 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
6181 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
6241 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
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