| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IGF1R-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001646 |
| 产品用途: | 3' AAVLINK plasmid for IGF1R expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
IGF1R
|
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IGF1R-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IGF1R-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IGF1R-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IGF1R-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IGF1R-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IGF1R-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IGF1R-FLAG 6845 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6845)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6845
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3558
/codon_start=1
/label=IGF1R
/translation="GLAAPAPKLKPRSRPRRRRLNTAKSLRISCTTPSSCPDLKGSGEM
SCKWPTPPCPAEAGTPRPQTPTTSPTRKSWRQSTLSLRAEWITRRELSFLTFGLSHCTA
SISTAATTRLRSWAAAPPTSSLQGLCPQKEQMTFLGQ*PGSQGLKTPSF*SGRNLRIPM
D*F*CMK*NTDHKLRISENVCPDRNTGSMEGPS*TG*TRGTTQPGFRPHLSLGMGRGQI
LCSSMSRPKQDMKTSSI*SSLCPSLSC*SWEGW*LCCTSSIEREITAGWGMECCMPL*T
RSTSALLMCTFLMSGRWLGRRSP*AGNLGRGRLGWSMKELPRVW*KMNLKPEWPLKQ*T
RPQACVRGLSFSTKLL**RSSIVTMWCDCWVWCPKASQHWSSWN**HGAISKVISGL*G
QKWRIIQS*HLQA*AR*FRWPERLQTAWHTSTPISSSTETLLPGIAW*PKISQSKSEIL
V*REISMRQTITGKEGKGCCPCAGCLLSPSRMESSPLTRTSGPSGSSSGRSPHWPSSPT
RACPTSKSFASSWRAAFWTSQTTVLTCCLN*CACAGSITPR*GLPSWRSSAASKRRWSL
ASGRSPSTTARRTSCPSRRSWTWSQRTWRASPWTPRPPRPPCHCPTDTQDTRPRTAPAL
GCWSSAPASTRDSLTPT*TGAARTSGPCRCPSLRP"
misc_feature 1595..1600
/label=SDS
misc_feature 1598..1603
/label=SDS
misc_feature 2961..2966
/label=SDS
misc_feature 3383..3388
/label=SDS
CDS 3565..3630
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3634..4065
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3778..3783
/label=SDS
repeat_region 4108..4248
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4108..4237
/label=AAV2 ITR
rep_origin 4323..4778
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5060..5164
/gene="bla"
/label=AmpR promoter
CDS 5165..6025
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6196..6784
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggcc ttgctgcgcc
1561 tgccccaaaa ctgaagccga gaagcaggcc gagaaggagg aggctgaata ccgcaaagtc
1621 tttgagaatt tcctgcacaa ctccatcttc gtgcccagac ctgaaaggaa gcggagagat
1681 gtcatgcaag tggccaacac caccatgtcc agccgaagca ggaacaccac ggccgcagac
1741 acctacaaca tcaccgaccc ggaagagctg gagacagagt accctttctt tgagagcaga
1801 gtggataaca aggagagaac tgtcatttct aaccttcggc ctttcacatt gtaccgcatc
1861 gatatccaca gctgcaacca cgaggctgag aagctgggct gcagcgcctc caacttcgtc
1921 tttgcaagga ctatgcccgc agaaggagca gatgacattc ctgggccagt gacctgggag
1981 ccaaggcctg aaaactccat ctttttaaag tggccggaac ctgagaatcc caatggattg
2041 attctaatgt atgaaataaa atacggatca caagttgagg atcagcgaga atgtgtgtcc
2101 agacaggaat acaggaagta tggaggggcc aagctaaacc ggctaaaccc ggggaactac
2161 acagcccgga ttcaggccac atctctctct gggaatgggt cgtggacaga tcctgtgttc
2221 ttctatgtcc aggccaaaac aggatatgaa aacttcatcc atctgatcat cgctctgccc
2281 gtcgctgtcc tgttgatcgt gggagggttg gtgattatgc tgtacgtctt ccatagaaag
2341 agaaataaca gcaggctggg gaatggagtg ctgtatgcct ctgtgaaccc ggagtacttc
2401 agcgctgctg atgtgtacgt tcctgatgag tgggaggtgg ctcgggagaa gatcaccatg
2461 agccgggaac ttgggcaggg gtcgtttggg atggtctatg aaggagttgc caagggtgtg
2521 gtgaaagatg aacctgaaac cagagtggcc attaaaacag tgaacgaggc cgcaagcatg
2581 cgtgagagga ttgagtttct caacgaagct tctgtgatga aggagttcaa ttgtcaccat
2641 gtggtgcgat tgctgggtgt ggtgtcccaa ggccagccaa cactggtcat catggaactg
2701 atgacacggg gcgatctcaa aagttatctc cggtctctga ggccagaaat ggagaataat
2761 ccagtcctag cacctccaag cctgagcaag atgattcaga tggccggaga gattgcagac
2821 ggcatggcat acctcaacgc caataagttc gtccacagag accttgctgc ccggaattgc
2881 atggtagccg aagatttcac agtcaaaatc ggagattttg gtatgacgcg agatatctat
2941 gagacagact attaccggaa aggagggaaa gggctgctgc ccgtgcgctg gatgtctcct
3001 gagtccctca aggatggagt cttcaccact tactcggacg tctggtcctt cggggtcgtc
3061 ctctgggaga tcgccacact ggccgagcag ccctaccagg gcttgtccaa cgagcaagtc
3121 cttcgcttcg tcatggaggg cggccttctg gacaagccag acaactgtcc tgacatgctg
3181 tttgaactga tgcgcatgtg ctggcagtat aaccccaaga tgaggccttc cttcctggag
3241 atcatcagca gcatcaaaga ggagatggag cctggcttcc gggaggtctc cttctactac
3301 agcgaggaga acaagctgcc cgagccggag gagctggacc tggagccaga gaacatggag
3361 agcgtccccc tggacccctc ggcctcctcg tcctccctgc cactgcccga cagacactca
3421 ggacacaagg ccgagaacgg ccccggccct ggggtgctgg tcctccgcgc cagcttcgac
3481 gagagacagc cttacgccca catgaacggg ggccgcaaga acgagcgggc cttgccgctg
3541 ccccagtctt cgacctgctg tacagactac aaagaccatg acggtgatta taaagatcat
3601 gacatcgact acaaggatga cgatgacaag taactcgaga taatcaacct ctggattaca
3661 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
3721 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
3781 ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc
3841 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa
3901 tttgtgatgc tattgcttta tttgtaacca tctagcttta tttgtgaaat ttgtgatgct
3961 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
4021 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagatc tggagaccgg
4081 tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
4141 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
4201 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
4261 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
4321 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
4381 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
4441 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
4501 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
4561 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
4621 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
4681 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
4741 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
4801 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
4861 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
4921 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
4981 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
5041 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
5101 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
5161 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
5221 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
5281 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
5341 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
5401 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
5461 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
5521 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
5581 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
5641 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
5701 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
5761 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
5821 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
5881 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
5941 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
6001 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
6061 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
6121 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
6181 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
6241 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
6301 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
6361 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
6421 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
6481 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
6541 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
6601 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
6661 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
6721 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
6781 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
6841 catgt
//
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