| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-ADGRL1-HA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001627 |
| 产品用途: | 3' AAVLINK plasmid for ADGRL1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ADGRL1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-ADGRL1-HA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-ADGRL1-HA载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-ADGRL1-HA质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-ADGRL1-HA载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-ADGRL1-HA质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-ADGRL1-HA质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-ADGRL1-HA 7288 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7288)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7288
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3971
/codon_start=1
/label=ADGRL1
/translation="VHTATMLLDVLEEGAFLLADNVREPARFLAAKENVVLEVTVLNTE
GQVQELVFPQEEYPRKNSIQLSAKTIKQNSRNGVVKVVFILYNNLGLFLSTENATVKLA
GEAGPGGPGGASLVVNSQVIAASINKESSRVFLMDPVIFTVAHLEDKNHFNANCSFWNY
SERSMLGYWSTQGCRLVESNKTHTTCACSHLTNFAVLMAHREIYQGRINELLLSVITWV
GIVISLVCLAICISTFCFLRGLQTDRNTIHKNLCINLFLAELLFLVGIDKTQYEIACPI
FAGLLHYFFLAAFSWLCLEGVHLYLLLVEVFESEYSRTKYYYLGGYCFPALVVGIAAAI
DYRSYGTEKACWLRVDNYFIWSFIGPVSFVIVVNLVFLMVTLHKMIRSSSVLKPDSSRL
DNIKSWALGAIALLFLLGLTWAFGLLFINKESVVMAYLFTTFNAFQGVFIFVFHCALQK
KVHKEYSKCLRHSYCCIRSPPGGTHGSLKTSAMRSNTRYYTGTQSRIRRMWNDTVRKQT
ESSFMAGDINSTPTLNRGTMGNHLLTNPVLQPRGGTSPYNTLIAESVGFNPSSPPVFNS
PGSYREPKHPLGGREACGMDTLPLNGNFNNSYSLRSGDFPPGDGGPEPPRGRNLADAAA
FEKMIISELVHNNLRGSSSAAKGPPPPEPPVPPVPGGGGEEEAGGPGGADRAEIELLYK
ALEEPLLLPRAQSVLYQSDLDESESCTAEDGATSRPLSSPPGRDSLYASGANLRDSPSY
PDSSPEGPSEALPPPPPAPPGPPEIYYTSRPPALVARNPLQGYYQVRRPSHEGYLAAPG
LEGPGPDGDGQMQLVTSL"
misc_feature 1516..1521
/label=SDS
misc_feature 1645..1650
/label=SDS
misc_feature 2750..2755
/label=SDS
misc_feature 3037..3042
/label=SDS
misc_feature 3184..3189
/label=SDS
misc_feature 3681..3686
/label=SDS
misc_feature 3733..3738
/label=SDS
CDS 3987..4013
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 4017..4043
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 4047..4073
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
terminator 4077..4508
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4221..4226
/label=SDS
repeat_region 4551..4691
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4551..4680
/label=AAV2 ITR
rep_origin 4766..5221
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5503..5607
/gene="bla"
/label=AmpR promoter
CDS 5608..6468
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6639..7227
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgcacacg gccaccatgc
1501 tcctcgacgt cctggaggag ggcgccttcc tgctggccga caatgtcagg gagcctgccc
1561 gcttcctggc tgccaaggag aacgtggtcc tggaggtcac agtcctgaac acagagggcc
1621 aggtgcagga gctggtgttc ccccaggagg agtacccgag aaagaactcc atccagctgt
1681 ctgccaaaac catcaagcag aacagccgca atggggtggt caaagttgtc ttcatcctct
1741 acaacaacct gggcctcttc ctgtccacgg agaatgccac agtgaagctg gccggcgaag
1801 caggcccggg tggccctggg ggcgcctctc tagtggtgaa ctcacaggtc atcgcagcat
1861 ccatcaacaa ggagtccagc cgcgtcttcc tcatggaccc tgtcatcttc accgtggccc
1921 acctggagga caagaaccac ttcaatgcta actgctcctt ctggaactac tcggagcgtt
1981 ccatgctggg ctactggtcg acccaaggct gccgcctggt ggagtccaac aagacccata
2041 ccacgtgtgc ctgcagccac ctcaccaact tcgctgtgct catggctcac cgtgagatct
2101 accagggccg catcaacgag ctgctgctgt cggtcatcac ctgggtgggc attgtgatct
2161 ccctggtctg cttggccatc tgcatctcca ccttctgctt cctgcggggg ctgcagaccg
2221 accgcaacac catccacaag aacctgtgca tcaacctctt cctggctgag ctgctcttcc
2281 tggtcgggat cgacaagact cagtatgaga ttgcctgccc catcttcgcc ggcctgctgc
2341 actatttctt cctggctgcc ttctcctggc tgtgcctgga gggcgtgcac ctctacctgc
2401 tactagtgga ggtgtttgag agcgagtatt cccgcaccaa gtactactac ctgggtggct
2461 actgcttccc ggccctggtg gtgggcatcg cggctgccat tgactaccgc agctacggca
2521 ccgagaaggc ctgctggctc cgagtggaca attacttcat ctggagtttc atcgggccag
2581 tctccttcgt tatcgtggtc aacctggtgt tcctcatggt gaccctgcac aagatgatcc
2641 gaagctcatc tgtgctcaag cccgactcca gccgcctgga caacattaaa tcctgggcgc
2701 tgggggccat cgcgctgctg ttcctgctgg gcctcacctg ggctttcggc ctcctcttca
2761 tcaacaagga gtcggtggtc atggcctatc tcttcaccac cttcaacgcc ttccaggggg
2821 tcttcatctt cgtctttcac tgcgccttac agaagaaggt gcacaaggag tacagcaagt
2881 gcctgcgtca ctcctactgc tgcatccgct ccccacccgg gggcactcac ggatccctca
2941 agacctcagc catgcgaagc aacacccgct actacacagg gacccagagc cgaattcgga
3001 ggatgtggaa tgacactgtg aggaaacaga cggagtcctc cttcatggcg ggtgacatca
3061 acagcacccc caccctgaac cgaggtacca tggggaacca cctgctgacc aaccccgtgc
3121 tgcagccccg tgggggcacc agtccctaca acaccctcat cgccgagtca gtgggcttca
3181 atccctcctc gccccctgtc ttcaactccc cagggagcta ccgggaaccc aagcacccct
3241 tgggaggccg ggaagcctgt ggcatggaca ccctgcccct gaacggcaac ttcaataaca
3301 gttactcctt gcgaagtggg gatttccctc ccggggatgg gggccctgag ccgccccgag
3361 gccggaacct agccgatgcg gcggcctttg agaagatgat catctcagag ctggtgcaca
3421 acaacctgcg ggggagcagc agcgcggcca agggccctcc accgcctgag ccccctgtgc
3481 cacctgtgcc agggggcggg ggcgaggaag aggcgggcgg gcccgggggt gctgaccggg
3541 ccgagattga acttctctat aaggccctgg aggagcctct gctgctgccc cgggcccagt
3601 cggtgctgta ccagagcgat ctggacgagt cggagagctg cacggccgag gacggcgcca
3661 ccagccggcc cctctcctcc cctcctggcc gggactccct ctatgccagc ggggccaacc
3721 tgcgggactc accctcctac ccggacagca gccctgaggg gcccagtgag gccctgcccc
3781 caccccctcc cgcacccccc ggcccccccg aaatctacta cacctcgcgc ccgccagccc
3841 tggtggcccg gaatcccctg cagggctact accaggtgcg gcgtcctagc cacgagggct
3901 acctggcagc cccaggcctt gaggggccag ggcccgatgg ggacgggcag atgcagctgg
3961 tcaccagtct ctgtacaggc ggaagctacc cgtatgatgt tccggattac gctggctatc
4021 cctacgacgt gcccgactat gccgggtacc cctatgacgt cccagactac gcataactcg
4081 agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
4141 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
4201 ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga
4261 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
4321 ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct
4381 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
4441 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
4501 ttttttaaag atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct
4561 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
4621 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
4681 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
4741 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
4801 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
4861 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
4921 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
4981 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
5041 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
5101 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
5161 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
5221 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
5281 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
5341 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
5401 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
5461 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
5521 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
5581 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
5641 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
5701 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
5761 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
5821 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
5881 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
5941 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
6001 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
6061 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
6121 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
6181 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
6241 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
6301 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
6361 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
6421 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
6481 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
6541 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
6601 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
6661 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
6721 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
6781 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
6841 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
6901 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
6961 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
7021 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
7081 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
7141 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
7201 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
7261 gccttttgct ggccttttgc tcacatgt
//
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