| 质粒名称: | pAAVLINK-EF1a-HA-5'-MSH6 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001641 |
| 产品用途: | 5' AAVLINK plasmid for MSH6 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
MSH6
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-MSH6
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-MSH6载体质粒基本信息
pAAVLINK-EF1a-HA-5'-MSH6质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-MSH6载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-MSH6质粒载体简介
pAAVLINK-EF1a-HA-5'-MSH6质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-MSH6 5466 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5466)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5466
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..2606
/codon_start=1
/label=MSH6
/translation="MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPG
GDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYP
WWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQKGG
HFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDKSEED
NEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSDEISSG
VGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAF
SAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVP
EDFLNSCTPGMRKWWQIKSQNFDLVICYKVGKFYELYHMDALIGVSELGLVFMKGNWAH
SGFPEIAFGRYSDSLVQKGYKVARVEQTETPEMMEARCRKMAHISKYDRVVRREICRII
TKGTQTYSVLEGDPSENYSKYLLSLKEKEEDSSGHTRAYGVCFVDTSLGKFFIGQFSDD
RHCSRFRTLVAHYPPVQVLFEKGNLSKETKTILKSSLSCSLQE"
misc_feature 1557..1562
/label=SDS
misc_feature 1869..1874
/label=SDS
misc_feature 1895..1900
/label=SDS
misc_feature 1898..1903
/label=SDS
misc_feature 2276..2281
/label=SDS
intron 2607..2647
/label=Splicing Donor
misc_feature 2651..2684
/label=lox2272-JT1571
repeat_region 2729..2869
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2729..2858
/label=AAV2 ITR
rep_origin 2944..3399
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3681..3785
/gene="bla"
/label=AmpR promoter
CDS 3786..4646
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4817..5405
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gtcgcgacag agcaccctgt acagcttctt
781 ccccaagtct ccggcgctga gtgatgccaa caaggcctcg gccagggcct cacgcgaagg
841 cggccgtgcc gccgctgccc ccggggcctc tccttcccca ggcggggatg cggcctggag
901 cgaggctggg cctgggccca ggcccttggc gcgctccgcg tcaccgccca aggcgaagaa
961 cctcaacgga gggctgcgga gatcggtagc gcctgctgcc cccaccagtt gtgacttctc
1021 accaggagat ttggtttggg ccaagatgga gggttacccc tggtggcctt gtctggttta
1081 caaccacccc tttgatggaa cattcatccg cgagaaaggg aaatcagtcc gtgttcatgt
1141 acagtttttt gatgacagcc caacaagggg ctgggttagc aaaaggcttt taaagccata
1201 tacaggttca aaatcaaagg aagcccagaa gggaggtcat ttttacagtg caaagcctga
1261 aatactgaga gcaatgcaac gtgcagatga agccttaaat aaagacaaga ttaagaggct
1321 tgaattggca gtttgtgatg agccctcaga gccagaagag gaagaagaga tggaggtagg
1381 cacaacttac gtaacagata agagtgaaga agataatgaa attgagagtg aagaggaagt
1441 acagcctaag acacaaggat ctaggcgaag tagccgccaa ataaaaaaac gaagggtcat
1501 atcagattct gagagtgaca ttggtggctc tgatgtggaa tttaagccag acactaagga
1561 ggaaggaagc agtgatgaaa taagcagtgg agtgggggat agtgagagtg aaggcctgaa
1621 cagccctgtc aaagttgctc gaaagcggaa gagaatggtg actggaaatg gctctcttaa
1681 aaggaaaagc tctaggaagg aaacgccctc agccaccaaa caagcaacta gcatttcatc
1741 agaaaccaag aatactttga gagctttctc tgcccctcaa aattctgaat cccaagccca
1801 cgttagtgga ggtggtgatg acagtagtcg ccctactgtt tggtatcatg aaactttaga
1861 atggcttaag gaggaaaaga gaagagatga gcacaggagg aggcctgatc accccgattt
1921 tgatgcatct acactctatg tgcctgagga tttcctcaat tcttgtactc ctgggatgag
1981 gaagtggtgg cagattaagt ctcagaactt tgatcttgtc atctgttaca aggtggggaa
2041 attttatgag ctgtaccaca tggatgctct tattggagtc agtgaactgg ggctggtatt
2101 catgaaaggc aactgggccc attctggctt tcctgaaatt gcatttggcc gttattcaga
2161 ttccctggtg cagaagggct ataaagtagc acgagtggaa cagactgaga ctccagaaat
2221 gatggaggca cgatgtagaa agatggcaca tatatccaag tatgatagag tggtgaggag
2281 ggagatctgt aggatcatta ccaagggtac acagacttac agtgtgctgg aaggtgatcc
2341 ctctgagaac tacagtaagt atcttcttag cctcaaagaa aaagaggaag attcttctgg
2401 ccatactcgt gcatatggtg tgtgctttgt tgatacttca ctgggaaagt ttttcatagg
2461 tcagttttca gatgatcgcc attgttcgag atttaggact ctagtggcac actatccccc
2521 agtacaagtt ttatttgaaa aaggaaatct ctcaaaggaa actaaaacaa ttctaaagag
2581 ttcattgtcc tgttctcttc aggaaggtaa gtatcaaggt tacaagacag gtttaaggag
2641 accaataagc tattattcgt ataggatact ttatacgaag ttattaagtt atggagaccg
2701 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
2761 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
2821 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
2881 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
2941 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
3001 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
3061 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
3121 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
3181 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
3241 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
3301 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
3361 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
3421 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
3481 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
3541 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
3601 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
3661 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
3721 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
3781 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
3841 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
3901 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
3961 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
4021 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
4081 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
4141 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
4201 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
4261 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
4321 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
4381 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
4441 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
4501 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
4561 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
4621 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
4681 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
4741 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
4801 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
4861 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
4921 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
4981 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
5041 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
5101 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
5161 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
5221 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
5281 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
5341 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
5401 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
5461 acatgt
//
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