AAV6-M2

LOCUS       AAV6-M2           7330 bp DNA     circular UNA 22-JUN-2026
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(740..760)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(740..758)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(779..795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(779..795)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(792..814)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    803..819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(827..857)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    872..893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1010..1027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1122..1838
                     /label=OriPromotor
     primer_bind     complement(1261..1280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1940..2800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2563..2582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2801..2905)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2931..3386)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3068..3089)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3280..3299
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3513..3535
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3527..3544
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3554..3573
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3554..3572
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3676..5535
                     /codon_start=1
                     /label=AAV-Rep2
                     /translation="PGFYEIVIKVPSDLDEHLPGISDSFVNWVAEKEWELPPDSDMDLN
                     LIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMVL
                     GRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQPE
                     LQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSAR
                     YMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAPD
                     YLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGKT
                     NIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKVR
                     VDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDFG
                     KVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPSTS
                     DAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSES
                     QPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"
     CDS             join(5555..7330,1..435)
                     /codon_start=1
                     /label=AAV-capM2
                     /translation="MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGL
                     VLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERL
                     QEDTSFGGNLGRAVFQAKKRVLEPFGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKTG
                     QQPAKKRLNFGQTGDSESVPDPQPLGEPPATPAAVGPTTMASGGGAPMADNNEGADGVG
                     NASGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISSASTGASNDNHYFGYSTPWG
                     YFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNDGVTTIANNLTST
                     VQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYF
                     PSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLNRTQNQSGSAQN
                     KDLLFSRGSPAGMSVQPKNWLPGPCYRQQRVSKTKTDNNNSNFTWTGASKYNLNGRESI
                     INPGTAMASHKDDKDKFFPMSGVMIFGKESAGASNTALDNVMITDEEEIKATNPVATER
                     FGTVATNHQSVNYEEETGDVHVMGALPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLM
                     GGFGLKHPPPQILIKNTPVPANPPAEFSATKFASFITQYSTGQVSVEIEWELQKENSKR
                     WNPEVQYTSNYAKSANVDFTVDNNGLYTEPRPIGTRYLTRPL"
ORIGIN
        1 accggagatg tgcatgttat gggagcctta cctggaatgg tgtggcaaga cagagacgta
       61 tacctgcagg gtcctatttg ggccaaaatt cctcacacgg atggacactt tcacccgtct
      121 cctctcatgg gcggctttgg acttaagcac ccgcctcctc agatcctcat caaaaacacg
      181 cctgttcctg cgaatcctcc ggcagagttt tcggctacaa agtttgcttc attcatcacc
      241 cagtattcca caggacaagt gagcgtggag attgaatggg agctgcagaa agaaaacagc
      301 aaacgctgga atcccgaagt gcagtataca tctaactatg caaaatctgc caacgttgat
      361 ttcactgtgg acaacaatgg actttatact gagcctcgcc ccattggcac ccgttacctc
      421 acccgtcccc tgtaattgct tgttaatcaa taaaccgttt aattcgtttc agttgaactt
      481 tggtctctgc gaagggcgaa ttcgtttaaa cctgcaggac tagaggtcct gtattagagg
      541 tcacgtgagt gttttgcgac attttgcgac accatgtggt cacgctgggt atttaagccc
      601 gagtgagcac gcagggtctc cattttgaag cgggaggttt gaacgcgcag ccgccaagcc
      661 gaattctgca gatatccatc acactggcgg ccgctcgact agagcggccg ccaccgcggt
      721 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
      781 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      841 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      901 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      961 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1021 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1621 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1741 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1861 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1921 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1981 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2041 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2101 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2161 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2221 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2281 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2341 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2401 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2461 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2521 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2581 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2641 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2701 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2761 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2821 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2881 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     2941 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3001 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3061 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3121 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3181 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3241 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3301 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3361 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     3421 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3481 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3541 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga
     3601 tcgaggtcga cggtatcggg ggagctcgca gggtctccat tttgaagcgg gaggtttgaa
     3661 cgcgcagccg ccatgccggg gttttacgag attgtgatta aggtccccag cgaccttgac
     3721 gagcatctgc ccggcatttc tgacagcttt gtgaactggg tggccgagaa ggaatgggag
     3781 ttgccgccag attctgacat ggatctgaat ctgattgagc aggcacccct gaccgtggcc
     3841 gagaagctgc agcgcgactt tctgacggaa tggcgccgtg tgagtaaggc cccggaggct
     3901 cttttctttg tgcaatttga gaagggagag agctacttcc acatgcacgt gctcgtggaa
     3961 accaccgggg tgaaatccat ggttttggga cgtttcctga gtcagattcg cgaaaaactg
     4021 attcagagaa tttaccgcgg gatcgagccg actttgccaa actggttcgc ggtcacaaag
     4081 accagaaatg gcgccggagg cgggaacaag gtggtggatg agtgctacat ccccaattac
     4141 ttgctcccca aaacccagcc tgagctccag tgggcgtgga ctaatatgga acagtattta
     4201 agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg cgcagcatct gacgcacgtg
     4261 tcgcagacgc aggagcagaa caaagagaat cagaatccca attctgatgc gccggtgatc
     4321 agatcaaaaa cttcagccag gtacatggag ctggtcgggt ggctcgtgga caaggggatt
     4381 acctcggaga agcagtggat ccaggaggac caggcctcat acatctcctt caatgcggcc
     4441 tccaactcgc ggtcccaaat caaggctgcc ttggacaatg cgggaaagat tatgagcctg
     4501 actaaaaccg cccccgacta cctggtgggc cagcagcccg tggaggacat ttccagcaat
     4561 cggatttata aaattttgga actaaacggg tacgatcccc aatatgcggc ttccgtcttt
     4621 ctgggatggg ccacgaaaaa gttcggcaag aggaacacca tctggctgtt tgggcctgca
     4681 actaccggga agaccaacat cgcggaggcc atagcccaca ctgtgccctt ctacgggtgc
     4741 gtaaactgga ccaatgagaa ctttcccttc aacgactgtg tcgacaagat ggtgatctgg
     4801 tgggaggagg ggaagatgac cgccaaggtc gtggagtcgg ccaaagccat tctcggagga
     4861 agcaaggtgc gcgtggacca gaaatgcaag tcctcggccc agatagaccc gactcccgtg
     4921 atcgtcacct ccaacaccaa catgtgcgcc gtgattgacg ggaactcaac gaccttcgaa
     4981 caccagcagc cgttgcaaga ccggatgttc aaatttgaac tcacccgccg tctggatcat
     5041 gactttggga aggtcaccaa gcaggaagtc aaagactttt tccggtgggc aaaggatcac
     5101 gtggttgagg tggagcatga attctacgtc aaaaagggtg gagccaagaa aagacccgcc
     5161 cccagtgacg cagatataag tgagcccaaa cgggtgcgcg agtcagttgc gcagccatcg
     5221 acgtcagacg cggaagcttc gatcaactac gcagacaggt accaaaacaa atgttctcgt
     5281 cacgtgggca tgaatctgat gctgtttccc tgcagacaat gcgagagaat gaatcagaat
     5341 tcaaatatct gcttcactca cggacagaaa gactgtttag agtgctttcc cgtgtcagaa
     5401 tctcaacccg tttctgtcgt caaaaaggcg tatcagaaac tgtgctacat tcatcatatc
     5461 atgggaaagg tgccagacgc ttgcactgcc tgcgatctgg tcaatgtgga tttggatgac
     5521 tgcatctttg aacaataaat gatttaaatc aggtatggct gccgatggtt atcttccaga
     5581 ttggctcgag gacaacctct ctgagggcat tcgcgagtgg tgggacttga aacctggagc
     5641 cccgaaaccc aaagccaacc agcaaaagca ggacgacggc cggggtctgg tgcttcctgg
     5701 ctacaagtac ctcggaccct tcaacggact cgacaagggg gagcccgtca acgcggcgga
     5761 tgcagcggcc ctcgagcacg acaaggccta cgaccagcag ctcaaagcgg gtgacaatcc
     5821 gtacctgcgg tataaccacg ccgacgccga gtttcaggag cgtctgcaag aagatacgtc
     5881 ttttgggggc aacctcgggc gagcagtctt ccaggccaag aagagggttc tcgaaccttt
     5941 tggtctggtt gaggaaggtg ctaagacggc tcctggaaag aaacgtccgg tagagcagtc
     6001 gccacaagag ccagactcct cctcgggcat tggcaagaca ggccagcagc ccgctaaaaa
     6061 gagactcaat tttggtcaga ctggcgactc agagtcagtc cccgacccac aacctctcgg
     6121 agaacctcca gcaacccccg ctgctgtggg acctactaca atggcttcag gcggtggcgc
     6181 accaatggca gacaataacg aaggcgccga cggagtgggt aatgcctcag gaaattggca
     6241 ttgcgattcc acatggctgg gcgacagagt catcaccacc agcacccgaa catgggcctt
     6301 gcccacctat aacaaccacc tctacaagca aatctccagt gcttcaacgg gggccagcaa
     6361 cgacaaccac tacttcggct acagcacccc ctgggggtat tttgatttca acagattcca
     6421 ctgccatttc tcaccacgtg actggcagcg actcatcaac aacaattggg gattccggcc
     6481 caagagactc aacttcaagc tcttcaacat ccaagtcaag gaggtcacga cgaatgatgg
     6541 cgtcacgacc atcgctaata accttaccag cacggttcaa gtcttctcgg actcggagta
     6601 ccagttgccg tacgtcctcg gctctgcgca ccagggctgc ctccctccgt tcccggcgga
     6661 cgtgttcatg attccgcagt acggctacct aacgctcaac aatggcagcc aggcagtggg
     6721 acggtcatcc ttttactgcc tggaatattt cccatcgcag atgctgagaa cgggcaataa
     6781 ctttaccttc agctacacct tcgaggacgt gcctttccac agcagctacg cgcacagcca
     6841 gagcctggac cggctgatga atcctctcat cgaccagtac ctgtattacc tgaacagaac
     6901 tcagaatcag tccggaagtg cccaaaacaa ggacttgctg tttagccggg ggtctccagc
     6961 tggcatgtct gttcagccca aaaactggct acctggaccc tgttaccggc agcagcgcgt
     7021 ttctaaaaca aaaacagaca acaacaacag caactttacc tggactggtg cttcaaaata
     7081 taaccttaat gggcgtgaat ctataatcaa ccctggcact gctatggcct cacacaaaga
     7141 cgacaaagac aagttctttc ccatgagcgg tgtcatgatt tttggaaagg agagcgccgg
     7201 agcttcaaac actgcattgg acaatgtcat gatcacagac gaagaggaaa tcaaagccac
     7261 taaccccgtg gccaccgaaa gatttgggac tgtggccaca aaccaccaga gtgttaatta
     7321 cgaagaagag
//