PCDH-CMV-mCherry-TRIM24

LOCUS       PCDH-CMV-mCherry-TRIM24             10302 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10302)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3220..3254
                     /label=TRIM24-F
     primer_bind     3220..3245
                     /label=TRIM19-F
     primer_bind     3220..3244
                     /label=TRIM18-F
     misc_feature    3240..6392
                     /label=TRIM24
     primer_bind     3892..3909
                     /label=24-seqF0
     primer_bind     4527..4543
                     /label=24-seqF1
     primer_bind     complement(4601..4619)
                     /label=24-seqR0
     primer_bind     5285..5303
                     /label=24-seqF3
     primer_bind     complement(6375..6412)
                     /label=TRIM24-R
     misc_feature    6399..6986
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6870..6881)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7059..7292
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7364..7485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7504..7639
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7677..7693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7701..7717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7725..7755)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7770..7791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8079..8667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8838..9698)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9699..9803)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10172..10185)
                     /label=Primer 5
     primer_bind     10277..10293
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggaggtggc ggtggagaag gcggtggcgg cggcggcagc ggcctcggct gcggcctccg
     3301 gggggccctc ggcggcgccg agcggggaga acgaggccga gagtcggcag ggcccggact
     3361 cggagcgcgg cggcgaggcg gcccggctca acctgttgga cacttgcgcc gtgtgccacc
     3421 agaacatcca gagccgggcg cccaagctgc tgccctgcct gcactctttc tgccagcgct
     3481 gcctgcccgc gccccagcgc tacctcatgc tgcccgcgcc catgctgggc tcggccgaga
     3541 ccccgccacc cgtccctgcc cccggctcgc cggtcagcgg ctcgtcgccg ttcgccaccc
     3601 aagttggagt cattcgttgc ccagtttgca gccaagaatg tgcagagaga cacatcatag
     3661 ataacttttt tgtgaaggac actactgagg ttcccagcag tacagtagaa aagtcaaatc
     3721 aggtatgtac aagctgtgag gacaacgcag aagccaatgg gttttgtgta gagtgtgttg
     3781 aatggctctg caagacgtgt atcagagctc atcagagggt aaagttcaca aaagaccaca
     3841 ctgtcagaca gaaagaggaa gtatctccag aggcagttgg tgtcaccagc cagcgaccag
     3901 tgttttgtcc ttttcataaa aaggagcagc tgaagctgta ctgtgagaca tgtgacaaac
     3961 tgacatgtcg agactgtcag ttgttagaac ataaagagca tagataccaa tttatagaag
     4021 aagcttttca gaatcagaaa gtgatcatag atacactaat caccaaactg atggaaaaaa
     4081 caaaatacat aaaattcaca ggaaatcaga tccaaaacag aattattgaa gtaaatcaaa
     4141 atcaaaagca ggtggaacag gatattaaag ttgctatatt tacactgatg gtagaaataa
     4201 ataaaaaagg aaaagctcta ctgcatcagt tagagagcct tgcaaaggac catcgcatga
     4261 aacttatgca acaacaacag gaagtggctg gactctctaa acaattggag catgtcatgc
     4321 atttttctaa atgggcagtt tccagtggca gcagtacagc attactttat agcaaacgac
     4381 tgattacata ccggttacgg cacctccttc gtgcaaggtg tgatgcatcc ccagtgacca
     4441 acaacaccat ccaatttcac tgtgatccta gtttctgggc tcaaaatatc atcaacttag
     4501 gttctttagt aatcgaggat aaagagagcc agccacaaat gcctaagcag aatcctgtcg
     4561 tggaacagaa ttcacagcca ccaagtggtt tatcatcaaa ccagttatcc aagttcccaa
     4621 cacagatcag cctagctcaa ttacggctcc agcatatgca gcaacaggta atggctcaga
     4681 ggcaacaggt gcaacggagg ccagcacctg tgggtttacc aaaccctaga atgcaggggc
     4741 ccatccagca accttccatc tctcatcagc aaccgcctcc acgtttgata aactttcaga
     4801 atcacagccc caaacccaat ggaccagttc ttcctcctca tcctcaacaa ctgagatatc
     4861 caccaaacca gaacatacca cgacaagcaa taaagccaaa ccccctacag atggctttct
     4921 tggctcaaca agccataaaa cagtggcaga tcagcagtgg acagggaacc ccatcaacta
     4981 ccaacagcac atcctctact ccttccagcc ccacgattac tagtgcagca ggatatgatg
     5041 gaaaggcttt tggttcacct atgatcgatt tgagctcacc agtgggaggg tcttataatc
     5101 ttccctctct tccggatatt gactgttcaa gtactattat gctggacaat attgtgagga
     5161 aagatactaa tatagatcat ggccagccaa gaccaccctc aaacagaacg gtccagtcac
     5221 caaattcatc agtgccatct ccaggccttg caggacctgt tactatgact agtgtacacc
     5281 ccccaatacg ttcacctagt gcctccagcg ttggaagccg aggaagctct ggctcttcca
     5341 gcaaaccagc aggagctgac tctacacaca aagtcccagt ggtcatgctg gagccaattc
     5401 gaataaaaca agaaaacagt ggaccaccgg aaaattatga tttccctgtt gttatagtga
     5461 agcaagaatc agatgaagaa tctaggcctc aaaatgccaa ttatccaaga agcatactca
     5521 cctccctgct cttaaatagc agtcagagct ctacttctga ggagactgtg ctaagatcag
     5581 atgcccctga tagtacagga gatcaacctg gacttcacca ggacaattcc tcaaatggaa
     5641 agtctgaatg gttggatcct tcccagaagt cacctcttca tgttggagag acaaggaaag
     5701 aggatgaccc caatgaggac tggtgtgcag tttgtcaaaa cggaggggaa ctcctctgct
     5761 gtgaaaagtg ccccaaagta ttccatcttt cttgtcatgt gcccacattg acaaattttc
     5821 caagtggaga gtggatttgc actttctgcc gagacttatc taaaccagaa gttgaatatg
     5881 attgtgatgc tcccagtcac aactcagaaa aaaagaaaac tgaaggcctt gttaagttaa
     5941 cacctataga taaaaggaag tgtgagcgcc tacttttatt tctttactgc catgaaatga
     6001 gcctggcttt tcaagaccct gttcctctaa ctgtgcctga ttattacaaa ataattaaaa
     6061 atccaatgga tttgtcaacc atcaagaaaa gactacaaga agattattcc atgtactcaa
     6121 aacctgaaga ttttgtagct gattttagat tgatctttca aaactgtgct gaattcaatg
     6181 agcctgattc agaagtagcc aatgctggta taaaacttga aaattatttt gaagaacttc
     6241 taaagaacct ctatccagaa aaaaggtttc ccaaaccaga attcaggaat gaatcagaag
     6301 ataataaatt tagtgatgat tcagatgatg actttgtaca gccccggaag aaacgcctca
     6361 aaagcattga agaacgccag ttgcttaaat aaggatccaa tcaacctctg gattacaaaa
     6421 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6481 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6541 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6601 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6661 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6721 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6781 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     6841 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6901 gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc
     6961 ggatctccct ttggccgcct ccccgccggt acctttaaga ccaatgactt acaaggcagc
     7021 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7081 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7141 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7201 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7261 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7321 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7381 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7441 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7501 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7561 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7621 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca
     7681 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7741 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7801 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7861 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7921 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7981 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8041 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8101 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8161 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8221 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8281 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8341 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8401 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8461 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8521 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8581 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8641 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8701 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8761 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8821 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8881 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8941 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9001 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9061 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9121 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9181 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9241 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9301 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9361 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9421 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9481 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9541 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9601 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9661 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9721 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9781 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     9841 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     9901 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     9961 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    10021 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    10081 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    10141 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    10201 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    10261 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//