| 质粒名称: | pCytERM-SNIFP-N1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000041 |
| 产品用途: | Expresses SNIFP in ER |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | p450, SNIFP |
| 物种来源: | H.sapiens, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
pCytERM-SNIFP-N1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCytERM-SNIFP-N1载体质粒基本信息
pCytERM-SNIFP-N1质粒图谱载体图谱和pCytERM-SNIFP-N1载体序列质粒序列多克隆位点信息
pCytERM-SNIFP-N1质粒载体简介
pCytERM-SNIFP-N1质粒序列载体序列
LOCUS pCytERM-SNIFP-N1 5049 bp DNA circular SYN 21-APR-2023
DEFINITION Vector for fusing EGFP to the C-terminus of a partner protein. For
other reading frames, use pEGFP-N2 or pEGFP-N3.
ACCESSION U55762
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5049)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Jun 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5049
/lab_host="Mammalian Cells"
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 615..701
/codon_start=1
/product="cytochrome P-450 IIC1, partial [Oryctolagus
cuniculus]
Sequence ID: AAA31436.1Length: 56Number of Matches: 1"
/label=cytochrome P-450
/translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
primer_bind 725..772
/label=0222-381-F
misc_feature 747..752
/label=Kozak
misc_feature 753..1712
/label=SNIFP
primer_bind complement(1690..1738)
/label=0222-381-R
polyA_signal 1837..1958
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1965..2420)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2447..2551
/gene="bla"
/label=AmpR promoter
promoter 2553..2910
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2761..2896
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2945..3739
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 3971..4018
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4347..4935
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctatggac cctgtggtgg tgctggggct ctgtctctcc tgtttgcttc
661 tcctttcact ctggaaacag agctatgggg gagggaagct tcgaattctg cagtcgacgg
721 taccgcgggc ccgggatcca ccggtcgcca ccatgtcccg tgaccctctc cccttcttcc
781 ctcccctgta cttgggtggc cccgagatta cgactgagaa ctgcgagagg gaacccatcc
841 acattccggg aagcatacag cctcatgggg cactcctgac tgccgatgga cactctggcg
901 aagtcctgca gatgagcctg aacgcagcta cctttcttgg ccaggagcca actgtgcttc
961 gagggcagac actggcggcc ctcctgcctg agcagtggcc tgccttgcaa gcggccctcc
1021 ctcccggatg tcccgatgcc ctccagtatc gggcgacact ggactggcca gccgcagggc
1081 atctcagtct gactgtccat cgggttggtg agctcttgat cctggagttt gagcccacgg
1141 aagcatggga ctctaccggt ccccatgccc ttagaaacgc catgtcagct ctcgagtctg
1201 caccaaacct gagagctctg gccgaggtcg caacccagac cgtgcgggag ctgacgggct
1261 tcgatcgtgt aatgctgtac aagttcgcac ccgatgctac aggcgaggtg atcgctgaag
1321 cgcgcagaga aggactgcac gcgtttctgg ggcataggtt tccggcttct ctgattcccg
1381 cacaagctag ggccctctat actcgccatc tgctgcggct taccgctgac acaagagccg
1441 cagctgtgcc tctggaccca gtcctgaatc cccagaccaa tgccccaacg cccctcggag
1501 gggctgtgct gcgcgccact agccccatgc acatgcagtt cctccggaat atgggcgtga
1561 ggtcctcact gagcgtaagt gtggtggtag gagggcaact gtggggcctg attgcctgtc
1621 accaccagac accttacgtg cttcctcctg atctgaggac aactctcgag tatctggggc
1681 gtgaactgtc cgaacaggtg caagtcaaag aataagcggc cgcgactcta gatcataatc
1741 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
1801 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
1861 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
1921 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
1981 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
2041 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
2101 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
2161 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
2221 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
2281 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
2341 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
2401 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
2461 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
2521 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
2581 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2641 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
2701 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
2761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
2821 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
2881 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
2941 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
3001 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
3061 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
3121 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
3181 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
3241 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
3301 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
3361 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
3421 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
3481 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
3541 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
3601 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
3661 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
3721 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
3781 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
3841 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
3901 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
3961 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
4021 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
4081 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
4141 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
4201 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
4261 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
4321 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
4381 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
4441 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
4501 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
4561 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
4621 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
4681 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
4741 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
4801 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
4861 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
4921 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
4981 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
5041 gccatgcat
//
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