| 质粒名称: | pCMV-TranC11a |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002684 |
| 产品用途: | Expresses TranC11a mammalian cells |
| 存储实验室: | Qi Biodesign（齐禾生科） |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | TranC11a |
| 物种来源: | Lawsonibacter sp. |
| 密码子优化: | 人源表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Directed evolution of a compact TranC11a system for efficient genome editing
BioRxiv, September 28, 2025
Zixu Zhu, Quan Gao, Qiang Gao, He Jia, Zhiwei Wang, Mingyang He, Lijuan Li, Lixiao Zhang, Shengnan Li, Shuai Jin, Caixia Gao and Kevin Tianmeng Zhao
|
pCMV-TranC11a
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCMV-TranC11a载体质粒基本信息
pCMV-TranC11a质粒图谱载体图谱和pCMV-TranC11a载体序列质粒序列多克隆位点信息
pCMV-TranC11a质粒载体简介
pCMV-TranC11a质粒序列载体序列
LOCUS pCMV-TranC11a 5244 bp DNA circular SYN 20-MAY-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS 2684-pCMV-TranC11a
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5244)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 7, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5244
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 445..465
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2230..2250
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2259..2276
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
polyA_signal 2305..2529
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(2600..2616)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2624..2640
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2648..2678)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2693..2714
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3002..3590)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3761..4621)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4622..4726)
/gene="bla"
/label=AmpR promoter
enhancer join(4996..5244,1..131)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcgt gtcttttagc
481 ctgaatgcca aaaagattcg gctggaaaac tacgccatga aaatgagact ctacccttct
541 cctacacagg ccgaacagat ggacaagatg ttcctggccc tgagactggc ctacaacatg
601 accttccacg aagtgtttca gcagaaccct gccgtgtgcg gcgaccccga cgaggacggc
661 aacgtgtggc ctagctacaa gaagatgacc aataagacct ggcggaaggc tctgatcgac
721 cagaatccag ccatcgccga ggctcccgca gccgccctga ccaccaacaa cggactgttc
781 ctgtcctgtg gccagcaggc ctggaagtac ggcatgcaca acctgccagc caacaaggct
841 gatagaaagg actttaggtt ttatagcctg cggaagccca gaagaagctt cgccgtgcag
901 atccgtccac gctgcatcat ccccagcgat accaaccaga aggtggccag aatcaagctg
961 cctaagatcg acggcgccat caaggccaga ggcttcaacc ggaagatctg gttcggcccc
1021 gacggaaaac atacatacga ggaagctctg gccgcccacg agctgtccaa caacctgacc
1081 gtgcgggtgt ccaaagatac atgcggcgat tacttcatct gcatcacctt ctcccagggc
1141 aaggtgaagg gcgacaagcc tacctgggag ttctaccagg aggtgagagt cagccctatc
1201 cccgagccca tcggcctgga cgtgggcatc aaggacatcg ccatcctgaa tacaggcaca
1261 aagtacgaga acaagcagtt caagcgggac agagccgcaa ccctgaaaaa gatgagccgg
1321 cagctgagca gaagatgggg ccctgccaac agcgctttcc gggactacaa caaaaacatc
1381 cgggccgaga acagagccct ggaacgggct caacaggacc ctggctcttc cggcgtggga
1441 cctgaggccc ctgtgctcaa gtctgtggcc caacctagca gaagatacct gaccatccag
1501 aaaaacagag ccaagctgga acgcaagatc gccagaagac gcgataccta ctaccaccag
1561 gtgacccgcg aggtggccgg aaagagcagc ctgctggccg tggaaaccct gcgggtcaag
1621 aacatgctgc agaaccacag actggccttc gcactgtctg atgctgctat gagcgacttc
1681 atcagcaagc tgaagtataa ggctcgaaga atccaggttc ctctggtggc catcggcacc
1741 tttcagccca gcagccagac atgtagcgtg tgcggctcca tcaaccccgc tgttaagaac
1801 ctgtctatca gagtgtggac ctgtcctaac tgtggaacaa gacacaatcg ggatatcaat
1861 gccgctaaga acatcctggc tatcgcacaa aacatgctgg aaaagaaggt gcccttcgcc
1921 gacgaggccc tgccagatgt gaagcctcct gccgctccag tgaagaaggc cgcccggaaa
1981 cctagggacg ccgtgttccc tgatcaccct gacctggtca tcagattcag caaggagctg
2041 acacagctca acgatcctag atacgtgatc gtgaacaagg ccaccaatca gattgtggac
2101 aacgcccaag gcgccggcta ccggagcgcc gccaaagcca agaactgcta caaagccaag
2161 ctggcctgga gcagcaagac aaacaagtct ggcggctcaa aaagaaccgc cgacggcagc
2221 gaattcgagc ccaagaagaa gaggaaagtc taaccggtca tcatcaccat caccattgag
2281 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
2341 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
2401 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
2461 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
2521 gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg acctctagct
2581 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
2641 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga
2701 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
2761 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
2821 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
2881 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
2941 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
3001 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
3061 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
3121 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
3181 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
3241 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
3301 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
3361 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
3421 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
3481 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
3541 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
3601 tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa gggattttgg
3661 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
3721 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
3781 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
3841 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
3901 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
3961 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
4021 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
4081 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
4141 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
4201 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
4261 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
4321 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
4381 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
4441 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
4501 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
4561 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
4621 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
4681 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
4741 aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc
4801 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
4861 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
4921 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
4981 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
5041 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
5101 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
5161 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
5221 acttggcagt acatcaagtg tatc
//
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