pGreenII-0800-LUC-bk1

LOCUS       pGreenII-0800-LUC-bk1            7171 bp DNA     circular SYN 23-MAR-2026
DEFINITION  pGREENII-0800.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        618..963
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    898..961
                     /label=mini35S
     misc_feature    1350..1372
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        1434..1779
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1798..2733
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    2788..2964
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    3108..3127
                     /label=pGreenII-LUC-F
     primer_bind     3128..3144
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3154..3172
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3220..3231)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3263..4915
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    3266..3283
                     /label=nLUCF
     misc_feature    complement(3329..3348)
                     /label=pGreenII-LUC-R
     misc_feature    4454..4471
                     /label=cLUCF
     misc_feature    4489..4510
                     /label=nLUCR
     polyA_signal    4977..5153
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5163..5187
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(5278..5866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6037..6852)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      join(7143..7171,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt atcgtacccc tactccaaaa atgtcaaaga tacagtctca
      601 gaagaccaaa gggctattga gacttttcaa caaagggtaa tttcgggaaa cctcctcgga
      661 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
      721 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatgcctc tgccgacagt
      781 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
      841 acgtcttcaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa
      901 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      961 acagcccaag ctctagccac catgacttcg aaagtttatg atccagaaca aaggaaacgg
     1021 atgataactg gtccgcagtg gtgggccaga tgttcgctaa tcaaacaggc ctaagctagt
     1081 acctttcgtc aaagttacag ctagtagttc aattgcatta gaatatatat atcaattatg
     1141 tcgcccttgg cttgtggaca atgcgctacg cgcaccggct ccgcccgtgg acaaccgcaa
     1201 gcggttgccc accgtcgagc gcctttgccc acaacccggc ggccggccgc aacagatcgt
     1261 tttataaatt tttttttttt gaaaaagaaa aagcccgaaa ggcggcaacc tctcgggctt
     1321 ctggatttcc gatccccgga attagatctt ggcaggatat attgtggtgt aacgttatcg
     1381 tacccctact ccaaaaatgt caaagataca gtctcagaag accaaagggc tattgagact
     1441 tttcaacaaa gggtaatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac
     1501 ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
     1561 ggaaaggcta tcattcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
     1621 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     1681 tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     1741 tcctctatat aaggaagttc atttcatttg gagaggacag cccaagctct agccaccatg
     1801 acttcgaaag tttatgatcc agaacaaagg aaacggatga taactggtcc gcagtggtgg
     1861 gccagatgta aacaaatgaa tgttcttgat tcatttatta attattatga ttcagaaaaa
     1921 catgcagaaa atgctgttat ttttttacat ggtaacgcgg cctcttctta tttatggcga
     1981 catgttgtgc cacatattga gccagtagcg cggtgtatta taccagacct tattggtatg
     2041 ggcaaatcag gcaaatctgg taatggttct tataggttac ttgatcatta caaatatctt
     2101 actgcatggt ttgaacttct taatttacca aagaagatca tttttgtcgg ccatgattgg
     2161 ggtgcttgtt tggcatttca ttatagctat gagcatcaag ataagatcaa agcaatagtt
     2221 cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg aatggcctga tattgaagaa
     2281 gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg ttttggagaa taacttcttc
     2341 gtggaaacca tgttgccatc aaaaatcatg agaaagttag aaccagaaga atttgcagca
     2401 tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt
     2461 gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac aaattgttag gaattataat
     2521 gcttatctac gtgcaagtga tgatttacca aaaatgttta ttgaatcgga cccaggattc
     2581 ttttccaatg ctattgttga aggtgccaag aagtttccta atactgaatt tgtcaaagta
     2641 aaaggtcttc atttttcgca agaagatgca cctgatgaaa tgggaaaata tatcaaatcg
     2701 ttcgttgagc gagttctcaa aaatgaacaa taattctagg gtacgctgaa atcaccagtc
     2761 tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag tagtttcccg
     2821 ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     2881 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     2941 aaatccagta ctaaaatcca gatcgataac attaacgctt acaatttcca ttcgccattc
     3001 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3061 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     3121 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     3181 gtcgactctc gaggatccgg tacccggtat cgatttaaaa ctagttctag agcggccgcc
     3241 accgcggtgg agatcgaatt ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc
     3301 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     3361 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     3421 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     3481 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     3541 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     3601 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     3661 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     3721 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     3781 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     3841 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     3901 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     3961 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     4021 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     4081 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     4141 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     4201 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     4261 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     4321 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     4381 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     4441 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     4501 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     4561 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     4621 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc
     4681 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     4741 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     4801 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     4861 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaattcta
     4921 gagaattcgg tacgctgaaa tcaccagtct ctctctacaa atctatctct ctctattttc
     4981 tccataaata atgtgtgagt agtttcccga taagggaaat tagggttctt atagggtttc
     5041 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     5101 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atccactagc
     5161 cttgacagga tatattggcg ggtaaactaa gtcgctgtat gtgtttgttt gagatctcat
     5221 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5281 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5341 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5401 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5461 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5521 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5581 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5641 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5701 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5761 cggaagaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5821 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5881 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5941 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6001 aatctaaagt atatatgtgt aacattggtc tagtgattag aaaaactcat cgagcatcaa
     6061 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     6121 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     6181 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     6241 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     6301 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     6361 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     6421 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     6481 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     6541 tttccctggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     6601 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     6661 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     6721 atacaatcgg tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     6781 atataaatca gcatccatgt tggaatttaa tcgcggcctt gagcaagacg tttcccgttg
     6841 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     6901 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct
     6961 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa
     7021 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa
     7081 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
     7141 ccccatccaa cagcccgccg tcgagcgggc t
//