pCambia1301-bk1

LOCUS       pCambia1301-bk1               11874 bp DNA     circular SYN 09-APR-2026
DEFINITION  1301.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(38..714)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    905..926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    909..934
                     /label=LacF
     promoter        941..971
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    979..995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1003..1019
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1028..1109
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             1059..1139
                     /codon_start=1
                     /label=Feature 24
                     /translation="GTRYRFKSRVDLQACKLGTGRRFTTS"
     primer_bind     complement(1113..1129)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1506..1851
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(1868..1882,2073..3890)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          1883..2072
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             3897..3914
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3949..4201
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4223..4247
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5548..6177
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6612..7679
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7745..7939
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8283..8423
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8609..9197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9284..10078)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10503..10527
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10605..10779
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10820..11845)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
ORIGIN
        1 tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa
       61 atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc
      121 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
      181 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
      241 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
      301 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
      361 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
      421 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttcacatca atccacttgc
      481 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
      541 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
      601 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
      661 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
      721 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
      781 accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga
      841 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      901 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      961 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
     1021 tgattacgaa ttcgagctcg gtcgactctc gaggatccgg tacccggtat cgatttaaat
     1081 ctagagtcga cctgcaggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac
     1141 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     1201 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     1261 ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttagc
     1321 ttcatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa
     1381 cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg
     1441 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg
     1501 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
     1561 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
     1621 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
     1681 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     1741 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     1801 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact
     1861 cttgaccatg gtagatctga gggtaaattt ctagtttttc tccttcattt tcttggttag
     1921 gacccttttc tctttttatt tttttgagct ttgatctttc tttaaactga tctatttttt
     1981 aattgattgg ttatggtgta aatattacat agctttaact gataatctga ttactttatt
     2041 tcgtgtgtct atgatgatga tgatagttac agaaccgacg actcgtccgt cctgtagaaa
     2101 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     2161 actgtggaat tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg
     2221 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg
     2281 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     2341 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     2401 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     2461 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     2521 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     2581 atgccggaat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     2641 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     2701 tggccaatgg tgatgtcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2761 gacaaggcac tagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2821 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2881 ttcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2941 cgttctactt tactggcttt ggtcgtcatg aagatgcgga cttgcgtggc aaaggattcg
     3001 ataacgtgct gatggtgcac gaccacgcat taatggactg gattggggcc aactcctacc
     3061 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     3121 tggtgattga tgaaactgct gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     3181 cgggcaacaa gccgaaagaa ctgtacagcg aagaggcagt caacggggaa actcagcaag
     3241 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     3301 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     3361 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     3421 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     3481 gttattacgg atggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     3541 aagaacttct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     3601 tggatacgtt agccgggctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     3661 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3721 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3781 agaaagggat cttcactcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac
     3841 gctggactgg catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa gctagccacc
     3901 accaccacca ccacgtgtga attacaggtg accagctcga atttccccga tcgttcaaac
     3961 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     4021 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     4081 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     4141 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     4201 cgggaattaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     4261 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     4321 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     4381 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     4441 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     4501 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     4561 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     4621 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     4681 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     4741 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     4801 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     4861 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     4921 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     4981 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     5041 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     5101 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     5161 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     5221 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     5281 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     5341 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     5401 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     5461 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     5521 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     5581 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     5641 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     5701 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     5761 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     5821 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     5881 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     5941 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     6001 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     6061 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     6121 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     6181 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     6241 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     6301 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     6361 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     6421 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     6481 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     6541 gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     6601 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     6661 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     6721 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     6781 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     6841 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     6901 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     6961 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     7021 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     7081 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     7141 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     7201 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     7261 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     7321 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     7381 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     7441 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     7501 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     7561 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     7621 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     7681 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     7741 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     7801 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     7861 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     7921 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     7981 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     8041 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     8101 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     8161 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     8221 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     8281 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     8341 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     8401 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8461 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8521 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8581 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8641 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8701 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8761 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8821 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8881 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8941 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9001 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9061 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9121 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9181 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9241 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     9301 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     9361 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     9421 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     9481 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     9541 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     9601 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     9661 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     9721 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     9781 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     9841 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     9901 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     9961 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
    10021 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
    10081 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    10141 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    10201 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    10261 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
    10321 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
    10381 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
    10441 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
    10501 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
    10561 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
    10621 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
    10681 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
    10741 cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgacag
    10801 atcccggtcg gcatctactc tatttctttg ccctcggacg agtgctgggg cgtcggtttc
    10861 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
    10921 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
    10981 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
    11041 tgcggagcat atacgcccgg agtcgtggcg atcctgcaag ctccggatgc ctccgctcga
    11101 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
    11161 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
    11221 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
    11281 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
    11341 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
    11401 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
    11461 ggctaagatc ggccgcagcg atcgcatcca tagcctccgc gaccggttgt agaacagcgg
    11521 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgaacggcgg gagatgcaat
    11581 aggtcaggct ctcgctaaac tccccaatgt caagcacttc cggaatcggg agcgcggccg
    11641 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
    11701 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
    11761 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
    11821 tcgcggtgag ttcaggcttt ttcatatctc attgcccccc ggatctgcga aagc
//