pCCLc-MND-A0201-SABR-Backbone

LOCUS       pCCLc-MND-A0201-SABR-Backbone               10927 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and 
            CD28-CD3z signaling domain. Contains a stuffer fragment flanked by 
            BsmBI sites at the epitope cloning site..
ACCESSION   .
VERSION     .
KEYWORDS    pCCLc-MND-A0201-SABR-Backbone
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Joglekar AV, Leonard MT, Jeppson JD, Swift M, Li G, Wong S, Peng S, 
  TITLE     T cell antigen discovery via signaling and antigen-presenting 
            bifunctional receptors
  JOURNAL   Nature Methods (2019) volume 16, pages 191–198.
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        37..67
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        255..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     789..809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             853..1033
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1080..1205
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1698..1931
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2116..2160
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2457..2574
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             7065..7130
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             7215..7244
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     LTR             7627..7860
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7932..8053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7969..7988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8023..8042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8093..8228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8155..8174
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8248..8267)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8249..8267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8277..8294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8277..8293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8286..8308)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8435..8890
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8522..8541)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8732..8753
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8916..9020
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9021..9881
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9239..9258)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10052..10640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10541..10560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10794..10811
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagcttg
      181 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
      241 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      841 tttagtgaac cggggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1021 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     1081 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1141 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1201 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1261 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1321 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1381 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1441 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1501 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1561 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1621 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1681 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1741 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1801 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1861 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1921 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1981 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     2041 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     2101 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     2161 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2221 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2281 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     2341 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     2401 aacggatctc gacggtatcg ataagctaat tcacaaatgg cagtattcat ccacaatttt
     2461 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2521 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     2581 tattacaggg acagcagaga tccagtttgg gaattagctt gatcgattag tccaatttgt
     2641 taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa
     2701 gcctatagag tacgagccat agatagaata aaagatttta tttagtctcc agaaaaaggg
     2761 gggaatgaaa gaccccacct gtaggtttgg caagctagga tcaaggttag gaacagagag
     2821 acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     2881 ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc
     2941 tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt
     3001 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     3061 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     3121 caataaaaga gcccacaacc cctcactcgg cgcgatctag atctcgaatc gaattcatgg
     3181 cgacgggttc aagaacttcc ctacttcttg catttggcct gctttgtttg ccgtggttac
     3241 aggagggctc ggcaggagac ggttgtaaat gagcacacaa aatacacatg ctaaaatatt
     3301 atattctatg acctttataa aatcaaccaa aatcttcttt ttaataactt tagtatcaat
     3361 aattagaatt tttatgttcc tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa
     3421 cgctagtttt agtaactcgc gttgttttct tcacctttaa taatagctac tccaccactt
     3481 gttcctaagc ggtcagctcc tgcttcaatc attttttgag catcttcaaa tgttctaact
     3541 ccaccagctg ctttaactaa agcattgtct ttaacaactg acttcattag tttaacatct
     3601 tcaaatgttg cacctgattt tgaaaatcct gttgatgttt taacaaattc taatccagct
     3661 tcaacagcta tttcacaagc tttcatgatt tcttcttttg ttaataaaca attttccata
     3721 atacatttaa caacatgtga tccagctgct ttttttacag ctttcatgtc ttctaaaact
     3781 aattcataat ttttgtcttt taatgcacca atatttaata ccatatcaat ttctgttgca
     3841 ccatctttaa ttgcttcaga aacttcgaat gcttttgtag ctgttgtgca tgcacctaga
     3901 ggaaaaccta caacatttgt tattcctaca tttgtgcctt ttaataattc tttacaatag
     3961 cttgttcaat atgaattaac acaaactgtt gcaaaatcaa attcaattgc ttcatcacat
     4021 aattgtttaa tttcagcttt cgtagcatct tgttttaata atgtgtgatc tatatatttg
     4081 tttagtttca ttttttctcc tatatattca tttttaattt taattcttta ataatttcgt
     4141 ctactttaac tttagcgttt tgaacagatt caccaacacc tataaaataa atttttagtt
     4201 taggttcagt tccacttggg cgaacagcaa atcatgactt atcttctaaa taaaatttta
     4261 gtaagtcttg tcctggcata ttatacattc catcgatgta gtcttcaaca ttaacaactt
     4321 taagtccagc aatttgagtt aagggtgttg ctctcaatga tttcattaat ggttcaattt
     4381 ttaatttctt ttcttctggt ttaaaattca agtttaaagt gaaagtgtaa tatgcaccca
     4441 tttctttaaa taaatcttct aaatagtcta ctaatgtttt attttgtttt ttataaaatc
     4501 aagcagcctc tgctattaat atagaagctt gtattccatc tttatctcta gctgagtcat
     4561 caattacata tccataactt tcttcataag caaaaacaaa atttaatccg ttatcttctt
     4621 ctttagcaat ttctctaccc attcatttaa atccagttaa agtttttaca atattaactc
     4681 catatttttc atgagcgatt ctatcaccca aatcacttgt tacaaaactt gaatatagag
     4741 ccggattttt tggaatgcta tttaagcgtt ttagatttga taattttcaa tcaattaaaa
     4801 ttggtcctgt ttgatttcca tctaatctta caaaatgacc atcatgtttt attgccattc
     4861 caaatctgtc agcatctggg tcattcataa taataatatc tgcatcatgt ttaataccat
     4921 attcaagcgg tatttttcat gcaggatcaa attctggatt tggatttaca acatttttaa
     4981 atgtttcatc ttcaaatgca tgctcttcaa cctcaataac gttatatcct gattcacgta
     5041 atatttttgg ggtaaattta gttcctgttc cattaactgc gctaaaaata atttttaaat
     5101 cttttttagc ttcttgctct tttttgtacg tctctggatg cggagggtcc ggcgggggag
     5161 gtagcggtgg tggaggaagc atccagcgta ctccaaagat tcaggtttac tcacgtcatc
     5221 cagcagagaa tggaaagtca aatttcctga attgctatgt gtctgggttt catccatccg
     5281 acattgaagt tgacttactg aagaatggag agagaattga aaaagtggag cattcagact
     5341 tgtctttcag caaggactgg tctttctatc tcttgtacta cactgaattc acccccactg
     5401 aaaaagatga gtatgcctgc cgtgtgaacc atgtgacttt gtcacagccc aagatagtta
     5461 agtgggatcg agacatggga ggtggtggca gcggtggagg gggctccggc ggtgggggat
     5521 ccggcggcgg cggaagcggc tctcactcca tgaggtattt cttcacatcc gtgtcccggc
     5581 ccggccgtgg ggagccccgc ttcatcgcag tgggctacgt ggacgacacg cagttcgtgc
     5641 ggttcgacag cgacgccgcg agccagagga tggagccgcg ggcgccgtgg atagagcagg
     5701 agggtccgga gtattgggac ggggagacac ggaaagtgaa ggcccactca cagactcacc
     5761 gagtggacct ggggaccctg cgcggctgct acaaccagag cgaggccggt tctcacaccg
     5821 tccagaggat gtatggctgc gacgtggggt cggactggcg cttcctccgc gggtaccacc
     5881 agtacgccta cgacggcaag gattacatcg ccctgaaaga ggacctgcgc tcttggaccg
     5941 cggcggacat ggcagctcag accaccaagc acaagtggga ggcggcccat gtggcggagc
     6001 agttgagagc ctacctggag ggcacgtgcg tggagtggct ccgcagatac ctggagaacg
     6061 ggaaagaaac actgcagcgc acggacgccc ccaaaacgca tatgactcac cacgctgtct
     6121 ctgaccatga agccaccctg aggtgctggg ccctgagctt ctaccctgcg gagatcacac
     6181 tgacctggca gcgggatggg gaggaccaga cccaggacac ggagctcgtg gagaccaggc
     6241 ctgcagggga tggaaccttc cagaagtggg cggctgtggt ggtgccttct ggacaggagc
     6301 agagatacac ctgccatgtg cagcatgagg gtttgcccaa gcccctcacc ctgagatggg
     6361 agccgtcttc ccagcccacc atccccatcg tgggcatcat tgctggcctg gttctctttg
     6421 gagctgtgat cactggagct gtggtcgctg ctgtgatgtg gaggaggaag agctcaggtg
     6481 gggaaggggt gaaggataga aaaggaggga gttacactca ggctgcaagc agtgacagtg
     6541 cccagggctc tgatgtgtct ctcacagctt gtaaagtgcg gagcaagagg tctcgcggtg
     6601 ggcattccga ctacatgaac atgaccccta gaaggcctgg cccaaccaga aagcactacc
     6661 agccatacgc ccctcccaga gatttcgccg cttatcgaag cgtgaagttc tcccgaagcg
     6721 cagatgcccc agcctatcag cagggacaga atcagctgta caacgagctg aacctgggaa
     6781 gacgggagga atacgatgtg ctggacaaaa ggcggggcag agatcctgag atgggcggca
     6841 aaccaagacg gaagaacccc caggaaggtc tgtataatga gctgcagaaa gacaagatgg
     6901 ctgaggccta ctcagaaatc gggatgaagg gcgaaagaag gagaggaaaa ggccacgacg
     6961 gactgtacca ggggctgagt acagcaacaa aagacaccta tgacgctctg cacatgcagg
     7021 ctctgccacc aagaagggca aaacgttcgg gttcgggtgc gccagtaaag cagacattaa
     7081 actttgattt gctgaaactt gcaggtgatg tagagtcaaa tccaggtcca ggggcaggtg
     7141 ccaccggccg cgccatggac gggccgcgcc tgctgctgtt gctgcttctg ggggtgtccc
     7201 ttggaggtgc caaggagcag aagctgatca gcgaggagga cctggagatc cctggccgtt
     7261 ggattacacg gtccacaccc ccagagggct cggacagcac agcccccagc acccaggagc
     7321 ctgaggcacc tccagaacaa gacctcatag ccagcacggt ggcaggtgtg gtgaccacag
     7381 tgatgggcag ctcccagccc gtggtgaccc gaggcaccac cgacaacctc atccctgtct
     7441 attgctccat cctggctgct gtggttgtgg gtcttgtggc ctacatagcc ttcaagaggt
     7501 gagaattcgt taacctcgag ggatcccccg gggtcgactg atcaaattcg agctcggtac
     7561 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7621 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     7681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7861 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7921 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7981 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8041 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     8101 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8161 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8221 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     8281 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8341 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8401 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     8461 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8521 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8581 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8641 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8701 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8761 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8821 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     8881 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8941 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9001 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9061 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9121 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9181 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     9241 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     9301 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9361 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9421 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9481 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9541 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9601 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9661 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9721 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9781 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9841 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9901 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9961 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10021 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10081 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10141 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10201 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10261 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    10321 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10381 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10441 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10501 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10561 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10621 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10681 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10741 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10801 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    10861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    10921 acgcaat
//